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Jingsong He,Ni Xie,Jianbo Yang,Hong Guan,Weicai Chen,Huisheng Wu,Zishan Yuan,Kun Wang,Guojin Li,Jie Sun,Limin Yu 한국유방암학회 2014 Journal of breast cancer Vol.17 No.3
Purpose: Synuclein-γ (SNCG), which was initially identified asbreast cancer specific gene 1, is highly expressed in advancedbreast cancers, but not in normal or benign breast tissue. Thisstudy aimed to evaluate the effects of SNCG siRNA-treatmenton breast cancer cells and elucidate the associated mechanisms. Methods: Vectors containing SNCG and negative control(NC) siRNAs were transfected into MDA-MB-231 cells; mRNAlevels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migrationwas assessed by the Transwell assay, apoptosis and cellcycle analyses were conducted with the flow cytometer, andWestern blot analysis was performed to determine the relativelevels of AKT, ERK, p-AKT, and p-ERK expression. Results:SNCG mRNA levels were significantly reduced in MDA-MB-231cells transfected with SNCG siRNA. Our results indicate that inSNCG siRNA-treated cells, cell migration and proliferation decreasedsignificantly, apoptosis was induced, and the cell cyclewas arrested. Western blot analysis indicated that the proteinlevels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups. Conclusion:SNCG siRNA could decrease the migration and proliferationof breast cancer cells by downregulating the phosphorylationof AKT and ERK.
Jinsong Yang,Jie Liu,Jingsong Xie 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.85 No.1
Impact event is the key factor influencing the operational state of the mechanical equipment. Additionally, nonlinear factors existing in the complex mechanical equipment which are currently attracting more and more attention. Therefore, this paper proposes a novel hybrid-separate identification strategy to solve the force identification problem of the nonlinear structure under impact excitation. The ‘hybrid’ means that the identification strategy contains both l1-norm (sparse) and l2-norm regularization methods. The ‘separate’ means that the nonlinear response part only generated by nonlinear force needs to be separated from measured response. First, the state-of-the-art two-step iterative shrinkage/thresholding (TwIST) algorithm and sparse representation with the cubic B-spline function are developed to solve established normalized sparse regularization model to identify the accurate impact force and accurate peak value of the nonlinear force. Then, the identified impact force is substituted into the nonlinear response separation equation to obtain the nonlinear response part. Finally, a reduced transfer equation is established and solved by the classical Tikhonove regularization method to obtain the wave profile (variation trend) of the nonlinear force. Numerical and experimental identification results demonstrate that the novel hybrid-separate strategy can accurately and efficiently obtain the nonlinear force and impact force for the nonlinear structure.
Jinsong Yang,Jianbin Cao,Jingsong Xie,Haixiao Zhao 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.80 No.1
A general approach is presented for the free vibration analysis of circular cylindrical shell resting on elastic foundation and subjected to classical boundary conditions of any type. The Winkler/Pasternak model is utilized to simulate the elastic foundation imposed on the cylindrical shell, and then it is easily to derive the potential energy of the elastic foundation. Based on the Flügge shell theory, explicit expressions for the mass and stiffness matrices are obtained. By taking the characteristic beam modal functions as the admissible functions, the Rayleigh-Ritz method is employed to derive the frequency equations of circular cylindrical shell with all the classical boundary conditions and resting on elastic foundation. Once the frequency equation has been determined, the frequencies can be calculated numerically. The excellent accuracy and validity of the present approach are demonstrated by numerical examples and comparisons with the results available in the literature. Finally, some further numerical results are given to illustrate the comprehensive effect of geometric properties and foundation coefficients on the frequencies of circular cylindrical shell in contact with elastic foundation.
Residual Mechanical Properties of Q235FR Steel Exposed to High-temperatures
Suhang Yang,Jingsong Sun,Zhifeng Xu 한국강구조학회 2024 International Journal of Steel Structures Vol.24 No.2
Steel structures are commonly used in engineering projects and infrastructure. Steel structures should have high bearing capacity and be able to resist fi re, earthquake, and corrosion. A new type of Q235 refractory steel has recently been developed in China, and the residual mechanical properties of steel structures are key indicators for estimating structural damage and reusability. Therefore, in-depth research is urgently needed. The yield stress, residual elastic modulus, ultimate tensile strength, and ultimate strain at room temperature to 900 °C and under air and water cooling conditions were detected. The residual mechanical properties of Q235FR steel were compared with other structural steels, and it was confi rmed through experiments that they are closely related to temperature and cooling methods. In the case of the above cooling methods, the loss of mechanical properties can be negligible when exposed to temperatures up to 600 °C. When the temperature exceeds 600 °C, the high temperature and cooling method signifi cantly impact the residual mechanical properties of Q235FR steel. The advantages of Q235FR steel are high strength, good ductility, strong corrosion resistance, and fi re resistance. The suggested predictive equations could be used to accurately evaluate the residual mechanical properties of Q235FR steel at high temperatures.
Bioactive polysaccharides isolated from the fruiting bodies of Phellinus sp.
Yan Yang,Jingsong Zhang,Yanfang Liu,Qingbo Wang,Qingjiu Tang,Shuai Zhou 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Phellinus sp. are assigned to the Basidiomycotina, Hymenomycetes, Aphyllophorales and Hymenochaetaceae, and have been shown containing various bioactive substances including triterpenoids, polysaccharides and flavones[1]. Traditional Chinese herbalists believe that Phellinus species are effective in treating many gynecopathic ailments[2] and are also reported to exhibit other pharmacological functions including tumor cell inhibition, antioxidant activity and anti-hepatic fibrosis effects[3]. Polysaccharides of Phellinus sp. has been reported to possess antitumor activities and inhibit tumor recrudescence and metastasis. There are little studies comparing the chemical composition and biological activities difference among polysaccharides from different Phellinus sp and little report about the pure polysaccharide structure analysis. In this study, eight kinds of crude polysaccharides were extracted from Phellinus fruit bodies and their chemical composition and bioactivities were researched. The polysaccharide and protein contents of eight crude polysaccharides had a certain extent differences. Monosaccharide composition and content of amino acids also existed some differences in eight crude polysaccharides. Eight different polysaccharides all showed enhancing splenocyte proliferation effect in vitro. PB-10P and JSHP had high cell proliferation rates with 50㎍/ml concentrations. The results indicated in some extent the immune activity of crude polysaccharides were correlation with the polysaccharide and protein content and composition of each sample. The crude polysaccharides of P. igniarius were further isolated and purified using DEAE Sepharose F. F. and gel-filtration chromatography (Sephacryl S-100-500 )repeatedly. Five water-soluble homogeneous polysaccharides (P60w1、P60s1、P1SP1、P10SP1and P100SP1) were obtained. Lack of absorption at 280 nm and 260 nm by UV scanning indicated that contained no protein and nucleic acid. HPLC produced a single symmetrical peak, indicated homogeneity and their molecular weigh were 1.71×104 Da、2.07×104 Da、1.48×104 Da、2.20×104 Da and 2.56×104 Da respectively. Structural of P60w-1 were determined using sugar and methylation analysis combined with 1H and 13C NMR spectroscopy, including COSY, TOCSY, NOESY, HSQC and HMBC experiments. The effect of P60w-1 on tumor growth was examined using subcutaneously transplanted H22 and Lewis Lung Carcinoma (LLC) tumor mouse models. Cyclophosphamide or Coriolus versicolor polysaccharopeptide served as positive controls in evaluating tumor response. Results showed that P60w-1 at the most effective dose of 100 mg/kg inhibited the growth of H22 and LLC by 48% and 37%, respectively.