Guhong injection protects against focal cerebral ischemia– reperfusion injury via anti-inflammatory effects in rats

Jinchao Ai,Haitong Wan,Mingchun Shu,Huifen Zhou,Tao Zhao,Wei Fu,Yu He 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.5

Guhong injection (GHI), composed of aceglutamideand safflower aqueous extract, has been used clinicallyfor the treatment of cerebrovascular diseases such ascerebral embolism, hemorrhage and mental deterioration. In this paper, we reported the results of the first study onthe anti-inflammatory effects of GHI in murine focalcerebral ischemia–reperfusion (I/R) injury. Adult male SDrats were randomly divided into six groups: Sham group,I/R group, GHI-L group (2.5 mL/kg), GHI-M group(5 mL/kg), GHI-H group (10 mL/kg) and Nimodipinegroup. I/R injury was induced by middle cerebral arteryocclusion (MCAO) for 1.5 h followed by reperfusion for24 h. Compared with I/R group, rats treated with GHIshowed dose dependent reductions in neurological defectscores and cerebral infarct volume. GHI obviously downregulatednitric oxide (NO), inducible NO synthase(iNOS), myeloperoxidase (MPO), interleukin-1b (IL-1b),TNF-a (tumor necrosis factor-a) and C reactive protein(CRP) levels in serum. Moreover, histological examinationby H&E staining showed that clear cell outline, less vacuolatedspaces and largely surviving neurons wereobserved in GHI-treated rats. The immunohistochemicalstaining revealed that GHI administration significantlydiminished the positive expressions of intercellular celladhesion molecule-1 (ICAM-1) and nuclear factor-jB p65(NF-jB p65) in brain tissues. Western blot analysis forICAM, NF-jB p65 and iNOS further solidified the abovefindings. All these results demonstrate that GHI exerts astrong and ameliorative effect on cerebral I/R injury in ratspossibly through the inhibition of inflammation.

Effect of Tb Doping on the Microstructure and Magnetic Properties of Quenched Fe73Ga27-xAlx Alloys

Jinchao Du,Peiying Jiao,Zhiguang Zhang,Pei Gong 한국자기학회 2024 Journal of Magnetics Vol.29 No.2

In this experiment, quenched Fe73Ga27-xAlx and (Fe73Ga27-xAlx)99.8Tb0.2 alloys (x=0, 1 , 2, 3, 4, 5) were respectively prepared, and the effects of 0.2 at% Tb doping on the microstructure and magnetic properties of Fe73Ga27-xAlx alloys were studied. The results show that the phase structure of Fe73Ga27-xAlx and (Fe73Ga27-xAlx)99.8Tb0.2 alloys are mainly composed of A2 phase, but (Fe73Ga27-xAlx)99.8Tb0.2 alloys also contain a small quantity of Tb2Fe17 phase. After Tb doping, the lattice constant of alloys is descended, altering the microstructure of alloys, and the orientation of (Fe73Ga27)99.8Tb0.2 and (Fe73Ga25Al2)99.8Tb0.2 a lloys a long the crystal direction [100] is increased saliently. The Al element in (Fe73Ga27-xAlx)99.8Tb0.2 alloy exists in the form of deposited phase, and Tb2Fe17 phase is formed at grain boundaries after Tb doping, thus improving the magnetostrictive property of alloys. The λ∥ and Vickers hardness of Fe73Ga27-xAlx alloys are in the range of 52-64×10-6 and 316.13-328.68 HV, separately. After Tb element doping, the λ∥ and Vickers hardness of the alloy reach the maximum 88×10-6 and 408.49 HV at x=2 at%, which is individually increased by 69.23 % and 29.21 % compared with Fe73Ga25Al2 alloy. And its Hc keeps low, which can reduce magnetization powers and losses. Therefore, (Fe73Ga27-xAlx)99.8Tb0.2 alloy gives consideration to the excellent magnetic properties and high Vickers hardness, having the most practical production value in the two series of alloys.

Learning Curve and Complications of Unilateral Biportal Endoscopy: Cumulative Sum and Risk-Adjusted Cumulative Sum Analysis

Jinchao Xu,Dong Wang,Jidan Liu,Chengyue Zhu,Jianhang Bao,Wenshuo Gao,Wei Zhang,Hao Pan 대한척추신경외과학회 2022 Neurospine Vol.19 No.3

Objective: The purpose of this study was to investigate the learning curve and complications of unilateral biportal endoscopy (UBE) in the treatment of lumbar disc herniation (LDH) and lumbar spinal stenosis (LSS). Methods: This was a retrospective cohort analysis of 197 consecutive patients who received UBE unilateral laminotomy bilateral decompression (UBE-ULBD) or lumbar discectomy (UBE-LD) surgery, including 107 males and 90 females with an average age of 64.83 ± 14.29 years. Cumulative sum (CUSUM) and risk-adjusted cumulative sum analysis (RA-CUSUM) were used to evaluate the learning curve, with the occurrence of complications defined as surgical failure, and variables of different phase of the learning curve were compared. Results: The cutoff point of learning curve of UBE surgery was 54 cases according to CUSUM analysis. The learning curve of UBE-ULBD and UBE-LD were divided into 3 phases. The first cutoff points were 31 and 12 cases, and the second cutoff point were 67 and 32 cases respectively. With the progress of the learning curve, the operation time and postoperative hospital stays decreased. The visual analogue scale and Oswestry Disability Index at the last follow-up were significantly lower than that before surgery. The incidence of surgical failure was 6.11% and began to decrease after the 89th case based on RA-CUSUM analysis. The surgical failure rate decreased from 10.11% to 2.78 after the 89th case with significant different. Conclusion: UBE surgery is effective in the treatment of LDH and LSS with low incidence of complications. But a learning curve of at least 54 cases still required for mastering UBE surgery.

Removal of NO from flue gas by wet scrubbing with NaClO2/(NH2)2CO solutions

Jinchao Wei,Ping Yu,Bo Cai,Houzhang Tan,Yunbai Luo 한국공업화학회 2009 Journal of Industrial and Engineering Chemistry Vol.15 No.1

The experiments were performed in a countercurrent packed column in a continuousmode to study the absorption of nitric oxide in sodium chlorite/urea solutions. Sodium chlorite mainly works as an agent to oxidize NO to NO2. A combined SO2/NO removal system was also tested. On the basis of high SO2 removal efficiency, the NO removal efficiencies under various experimental conditions were emphatically measured. Among the operating variables such as initial NaClO2 concentration, urea concentration, temperature and initial pH value, the pH value of the absorbing liquid was found to have a great impact on both NO removal efficiency and NO2 concentration. NO removal efficiency was increased with increasing NaClO2 concentration and temperature. Urea almost has no negative effect on NO removal efficiency, however it aids the abatement of NO2 greatly. The anions in the spent scrubbing liquor were analyzed by ion chromatography. 2009 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

Decision-making for Connected and Automated Vehicles in Chanllenging Traffic Conditions Using Imitation and Deep Reinforcement Learning

Hu Jinchao,Li Xu,Hu Weiming,Xu Qimin,Huyue Sun 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.6

Decision-making is the “brain” of connected and automated vehicles (CAVs) and is vitally critical to the safety of CAVs. The most of driving data used to train the decision-making algorithms is collected in general traffic conditions. Existing decision-making methods are difficult to guarantee safety in challenging traffic conditions, namely severe congestion and accident ahead. In this context, a semi-supervised decision-making algorithm is proposed to improve the safety of CAVs in challenging traffic conditions. To be specific, we proposed the expert-generative adversarial imitation learning (E-GAIL) that integrates imitation learning and deep reinforcement learning. The proposed E-GAIL is deployed in roadside unit (RSU). In the first stage, the decision-making knowledge of the expert is imitated using the real-world data collected in general traffic conditions. In the second stage, the generator of E-GAIL is further reinforced and achieves self-learn decision-making in the simulator with challenging traffic conditions. The E-GAIL is tested in general and challenging traffic conditions. By comparing the evaluation metrics of time to collision (TTC), deceleration to avoid a crash (DRAC), space gap (SGAP) and time gap (TGAP), the E-GAIL greatly outperforms the state-of-the-art decision-making algorithms. Experimental results show that the E-GAIL not only make-decision for CAVs in general traffic conditions but also successfully enhances the safety of CAVs in challenging traffic conditions.

Performance characteristics of NO removal by cobalt diethylenetriamine solution

Houzhang Tan,Jinchao Wei,Yunbai Luo,Ping Yu 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.3

The cobalt(II) diethylenetriamine ([Co(dien)2]2+) complex is a newly developed metal thiochelate for the removal of NO from flue gas. The performance characteristics of NO absorption into [Co(dien)2]2+ solution were studied in a stirred reactor. The experimental results showed that this absorption could be regarded as fast pseudo-mth-order reaction and the reaction rate could be expressed as second-order with respect to NO concentration and first-order with respect to [Co(dien)2]2+ concentration. The enhancement factor was 1609.1 at the [Co(dien)2]2+ concentration of 0.01mol/L. Its optimal absorption conditions were temperature 50 oC, NO concentration 540 ppm, [Co(dien)2]2+ concentration 0.02 mol/L and O2 concentration 6%, which were determined by orthogonal experiment.

LncRNA PART1 Attenuates Myocardial Ischemia-Reperfusion Injury by Regulating TFAP2C/DUSP5 Axis via miR-302a-3p

Min Zeng,Xin Wei,Jinchao Zhou,Siqi Luo The Korean Society of Cardiology 2024 Korean Circulation Journal Vol.54 No.2

Background and Objectives: Myocardial ischemia-reperfusion injury (MIRI) refers to the damage of cardiac function caused by restoration of blood flow perfusion in ischemic myocardium. However, long non-coding RNA prostate androgen regulated transcript 1 (PART1)'s role in MIRI remain unclear. Methods: Immunofluorescence detected LC3 expression. Intermolecular relationships were verified by dual luciferase reporter assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry and transferase-mediated dUTP nick-end labeling (TUNEL) assays analyzed cell viability and apoptosis. The release of lactate dehydrogenase was tested via enzyme-linked immunosorbent assay (ELISA). Left anterior descending coronary artery surgery induced a MIRI mouse model. Infarct area was detected by 2,3,5-triphenyltetrazolium chloride staining. Hematoxylin and eosin staining examined myocardial injury. ELISA evaluated myocardial marker (creatine kinase MB) level. Results: PART1 was decreased in hypoxia/reoxygenation (H/R) induced AC16 cells and MIRI mice. PART1 upregulation attenuated the increased levels of Bax, beclin-1 and the ratio of LC3II/I, and enhanced the decrease of Bcl-2 and p62 expression in H/R-treated cells. PART1 upregulation alleviated H/R-triggered autophagy and apoptosis via miR-302a-3p. Mechanically, PART1 targeted miR-302a-3p to upregulate transcription factor activating enhancer-binding protein 2C (TFAP2C). TFAP2C silencing reversed the protected effects of miR-302a-3p inhibitor on H/R treated AC16 cells. We further established TFAP2C combined to dual-specificity phosphatase 5 (DUSP5) promoter and activated DUSP5. TFAP2C upregulation suppressed H/R-stimulated autophagy and apoptosis through upregulating DUSP5. Overexpressed PART1 reduced myocardial infarction area and attenuated MIRI in mice. Conclusion: PART1 improved the autophagy and apoptosis in H/R-exposed AC16 cells through miR-302a-3p/TFAP2C/DUSP5 axis, which might provide novel targets for MIRI treatment.

The Silurian−Devonian granitoids in the East Kunlun orogenic belt, northern Qinghai-Tibetan plateau, China: origin and tectonic implications

Namkha Norbu,Yuegao Liu,Jinchao Li,Qunzi Jia,Wei Wang,Xiaobing Song,Yazhi Li,Huilei Kong,Yijie Gao,Xianzheng Guo,Xuegang Yao 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.6

The East Kunlun orogenic belt (EKOB) is located in the northeastern part of the Qinghai-Tibetan Plateau, China. Voluminous Silurian–Devonian granitoids were formed during the Proto-Tethys Ocean subduction and collision process. In this study, zircon U-Pb age dating, in-situ Hf isotope analyses, and whole rock major and trace element analyses have been carried out on granitoids from the central to eastern EKOB. The formation ages of granodiorite in the Bairiqili area, the granodiorite in the Wulonggou area are 410 ± 2 Ma and 396 ± 1 Ma, respectively, and the granodiorites show I-type features. The Wulonggou syenogranite with a formation age of 398 ± 2 Ma has a high differentiation index (D.I. = 89.59–92.43), and may belong to the high fractionated I-type granite. The Lalangmai tonalite with a formation age of 423 ± 2 Ma has high Sr (339–590 μg/g), εHf(t) (1.5–7.3), and low Y (3.45–6.33 μg/g), which are similar to modern adakites. The Lalangmai tonalite is the latest adakite record during the Proto-Tethys evolution in EKOB. Combined with previous studies, it is concluded that the granitoids from Cambrian to Devonian divided into four episodes. The first episode (515–436 Ma) mainly consists of I-type island arc granites and adakite, which are formed in a subduction environment. The second episode (436–427 Ma) is characterized by the S-type granites related to the collision. The third episode (427–420 Ma) is the transition period between the collision and the post-collision environment. In the fourth episodes (420–385 Ma), granitoids were formed in post-collision extensional environment, which is characterized by I-type and A-type granites. The upwelling of asthenosphere material is likely caused by the collapse of the orogenic belt. After 420 Ma, I-type and A-type granites formed at different depths but in the same environment.

A Study on the Effect of Professional Identity on Core Literacy of Chinese Normal University Students

진막비(MuFei Chen),임진초(JinChao Lin),우련의(LianYi Yu),고흥(Heung Kou) 한국정치사회연구소 2024 한국과 국제사회 Vol.8 No.4

Deep electrical structure and dynamic mechanism of the Yinchuan Graben on the western margin of the Ordos Block

Gang Min,Bingxi Yin,Jinchao Chen,Yong Yang,Huiling Yuan,Yan Wang 한국지질과학협의회 2022 Geosciences Journal Vol.26 No.1

The Yinchuan Graben (YG) is situated in the transitional area between the Tibetan Plateau, Ordos Block (OB) and Alxa Block (AB). The dynamic mechanism of the YG is controversial because of its unique tectonic environment. This study used a broadband magnetotelluric profile constrained by high-density point spacing to reliably obtain the deep electrical structure across the Helanshan Orogenic Belt and YG and to discuss the dynamic mechanism of the YG. The main research results are as follows. 1) Our profile and previous seismic profiles show a low-resistivity and low-velocity body in the deep YG, which extends upward and cuts through the bottom boundary of the upper crust, and all results indicate that this body may be the result of partial melting due to upwelling mantle material. 2) Our profile also shows a high-resistivity anomaly in the middle–lower crust of the Helanshan orogenic belt, which can be interpreted as intrusive rocks formed by the emplacement of partially melted materials. 3) The Helanshan east piedmont fault dips eastward and intersects with the Yellow River fault at the depth of 20 km, and the typical structure of the intersection may be the seismogenic structure of the Pingluo Ms8.0 earthquake. 4) The evolution of the YG may have been controlled by various dynamic mechanisms, and the initial formation of the YG may have resulted from the upwelling mantle material beneath the northern OB; the evolution of the YG since 10 Ma may also have been affected by lateral mantle flow from the Tibetan Plateau to the gap between the OB and AB.