Association of CAST Gene Polymorphisms with Carcass and Meat Quality Traits in Chinese Commercial Cattle Herds

Li, Jiao,Zhang, Lu-Pei,Gan, Qian-Fu,Li, Jun-Ya,Gao, Hui-Jiang,Yuan, Zheng-Rong,Gao, Xue,Chen, Jin-Bao,Xu, Shang-Zhong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.11

Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.

Study on the Crystalline Structure and the Thermal Stability of Silver-oxide Films Deposited by Using Direct-current Reactive Magnetron Sputtering Methods

Gao Xiao-Yong,Feng Hong-Liang,Zhang Zeng-Yuan,Ma Jiao-Min 한국물리학회 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.56 No.4

Silver-oxide (AgxO) films were deposited on glass substrates by direct-current reactive magnetron sputtering at different oxygen flux ratios (OFR = [O2]/[Ar]) and substrate temperatures (Ts). An X-ray diffraction analysis indicates that the AgxO films are biphased (Ag + Ag2O) when deposited at low OFR values and that Ag2O-dominated AgxO film can only be synthesized at higher OFR values, as con¯rmed by X-ray photoelectron spectroscopy. This result may be due to the Ag2O phase being preferably produced at high OFR value. The AgO phase is thermodynamically unsta-ble compared with the Ag2O phase. In order to further offer deep insight into the ¯lm's thermal stability, Ag2O-dominated AgxO ¯lms were thermally treated by using a rapid thermal processing technique at different annealing temperatures for different annealing times. The Ag2O phase is thermodynamically stable at temperatures below the threshold of the thermal decomposition tem-perature which approaches 175 ±C. The domination of the Ag2O phase in the AgxO film may be attributed to the chain reaction AgO → Ag2O ↔ Ag + O, AgO + Ag → Ag2O.

Physicochemical properties, multi-elemental composition, and antioxidant activity of five unifloral honeys from Apis cerana cerana

Jiao Wu,Shan Zhao,Xin Chen,Yuanda Jiu,Junfeng Liu,Jinglin Gao,Shijie Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.13

Honey quality is in relation to botanical origin, and physicochemical properties, elemental composition, and antioxidant activity have been used for assessment and identification of honeys. The goal of this study is to contribute to the general analysis of five unifloral honeys from Cocos nucifera L., Dalbergia benthami Prain, Bombax ceiba L., Castanea mollissima Bl., and mangrove in Hainan province, China. Our results revealed that B. ceiba honey had the highest pH (4.27), color (139.33 mm Pfund), ash content (1.03 g/100 g), and electrical conductivity (1312.00 μS/cm) in five unifloral honeys. Furthermore, B. ceiba honey also contained the highest levels of total phenolic content (75.54 mg GAE/100 g) and total flavonoid content (29.22 mg RE/100 g), as well as the strongest antioxidant activity (DPPH IC50 value, 3.97 mg/mL; FRAP value, 6527.43 µmol TE/kg). Moreover, we revealed a considerable variation in element contents in honeys using ICP-MS, with potassium being the most predominant element. B. ceiba honey had the highest contents of K, Ca, Mg, and P, whereas the highest amount of Na was found in mangrove honey. Overall, our data indicated that B. ceiba honey deserves further research as a potential antioxidant agent.

Surface third and fifth harmonic generation at crystalline Si for non-invasive inspection of Si wafer’s inter-layer defects

Gao, Yi,Lee, Hyub,Jiao, Jiannan,Chun, Byung Jae,Kim, Seungchul,Kim, Dong-Hwan,Kim, Young-Jin The Optical Society 2018 Optics express Vol.26 No.25

CuO-doped Ce for catalytic wet peroxide oxidation degradation of quinoline wastewater under wide pH conditions

Zhaojie Jiao,Xianming Zhang,Haifeng Gong,Donglin He,Hong Yin,Yunqi Liu,Xu Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

This study aimed to treat toxic and refractory organic wastewater, and to address the limitations ofFenton method in treating organic wastewater, that the active components are easy to lose and usuallyoperate at the pH value of 2 3. In the present study, CuCe oxide catalyst was produced through citricacid-assisted complexation. Besides, the structural stability and catalytic wet peroxide oxidation (CWPO)performance of the catalyst after 5 times of application were delved into by characterization. The effectsof catalyst dosage and pH on quinoline removal were ascertained, while the reusability of the catalystwas explored. Moreover, the variations of pH, hydroxyl radical concentration and UV–Vis spectra inthe reaction system were analyzed. The possible degradation pathway and the catalytic mechanism werealso discussed. As suggested from the results, the catalyst exhibited high catalytic activity, structural stabilityand pH adaptability; it also had high CWPO performance for quinoline at the pH from 3.8 to 10.5,and the pH value need not be regulated. Under optimal conditions, 98.1% of quinoline and 86.1% of totalorganic carbon (TOC) were removed. In the CWPO process of quinoline, the OH attacked the nitrogen ringand the benzene ring in sequence.

Prenylated indole diketopiperazine alkaloids from a mangrove rhizosphere soil derived fungus Aspergillus effuses H1-1

Huquan Gao,Tian-Jiao Zhu,De-Hai Li,Qian Qun Gu,Weizhong Liu 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.8

One new prenylated indole diketopiperazinealkaloid, named dihydroneochinulin B (1), one known spiropolyketide-diketopiperazine hybrid cryptoechinuline D (2)and three related known metabolites didehydroechinulin B(3), neoechinulin B (4) and auroglaucin (5) were isolatedfrom the mangrove rhizosphere soil derived fungus, Aspergilluseffuses H1-1. The structures were assigned by detailedspectroscopic analysis. The enantiomers of cryptoechinulineD (2) were separated to be (?)-cryptoechinuline D (2a) and(-)-cryptoechinuline D (2b) by chiral HPLC, and theirabsolute configurations were determined by ECD analysis. The cytotoxic effects of the compounds were preliminarilyevaluated on P388, HL-60, BEL-7402 and A-549 cell linesby SRB or MTT methods, and compounds 2, 2a and 3showed significant activities.

Improved performance of polyamide nanofiltration membranes by incorporating reduced glutathione during interfacial polymerization

Zhiwei Jiao,Linjie Zhou,Mengyuan Wu,Kang Gao,Yanlei Su,Zhongyi Jiang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.12

Inspired by the specific amino acid sequence Asn-Pro-Ala (NPA) of water channel aquaporins (AQPs), we fabricated polyamide (PA) nanofiltration (NF) membranes by introducing reduced glutathione (GSH) in interfacial polymerization (IP) method. Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectrometry (XPS), scanning electron microscope (SEM), atomic force microscopy (AFM), zeta potential and static water contact angle measurement were employed to characterize the chemical composition, morphology, electronegativity and hydrophilicity of the NF membranes. The water flux of GSH/PIP-TMC NF membrane reached 32.00 L m2 h1 at 0.2MPa, which was approximately twice than that of pristine PIP-TMC NF membrane when the ratio of GHS to piperazidine (PIP) was 40% during IP process. More water channels were built as GSH was embedded into PA layer. The fabricated NF membranes also took on potent rejection for dyes and Na2SO4. This study presents a simple and facile method to simulate water channels-based biological materials which may find potential application in water treatment.

Research on feature enhancement method of weak fault signal of rotating machinery based on adaptive stochastic resonance

Kangping Gao,Xinxin Xu,Jiabo Li,Shengjie Jiao,Ning Shi 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.2

Aiming at the problem that the traditional filtering method will filter out some useful signals when extracting weak fault features of rotating machinery, resulting in the loss of characteristic signals, a method for extracting weak fault features based on sin-cosine algorithm (SCA) is proposed. Combining the sensitivity of kurtosis to impact signals and correlation coefficients to interference noise, the paper proposes a new stochastic resonance (SR) performance evaluation index-weighted power spectrum kurtosis (WPSK), which solves the shortcoming of the traditional evaluation index that the fault frequency needs to be known in advance. The structural parameters of the SR are optimized by the SCA to improve the “resonance” effect. The SCA-based SR method is applied to the weak feature extraction of faulty bearings and compared with the SR model of particle swarm optimization, the results show that when the bearing inner-race fails, the value of WPSK increases by 33.5 %, and when the outerrace fails, the value of WPSK increases by 44.1 %.

Highly Dispersible Cellulose Nanofibrils Produced via Mechanical Pretreatment and TEMPO-mediated Oxidation

Liang Jiao,Huiyang Bian,Ying Gao,Xuliang Lin,Wenyuan Zhu,Hongqi Dai 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11

Cellulose nanofibrils (CNFs) can prepare flexible nanomaterials with its large aspect ratio. Due to the supramolecular, cellulose fibers are difficultly dissociated to CNFs. In order to destroy this supramolecular structure and prepare high dispersible CNFs, the mechanical and chemical treatments are required. This study examined the effects of mechanical pretreatment and TEMPO-oxidation on the properties of CNFs preparing from bleached softwood kraft pulp. The total yield of TEMPO-oxidized cellulose nanofibrils (TOCNFs) reached 85 %. The morphology, carboxyl group content, crystallinity, surface charge, self-assembling properties, and optical transmittance of the corresponding TOCNFs suspension were investigated. The transmittance of TOCNFs suspension was 95.1 % at 600 nm wavelength and its corresponding ζ- potential was -61.3 mV, indicated that the strong electrostatic repulsion between TOCNFs was the most significant factor on the highly transparent TOCNFs suspension in water. Furthermore, the mostly negative charged TOCNFs obtained by freeze drying assembled into the finest and most uniform networked structure. Such highly transparent, functionalized, selfassembled cellulose nanofibrils are favorable in transparent films, flexible displays and electrochemistry materials.

Vibration analysis of aero parallel-pipeline systems based on a novel reduced order modeling method

Peixin Gao,Yuanlin Zhang,Xuefeng Liu,Tao Yu,Jiao Wang 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.8

The modeling of parallel-pipeline is a difficult work in engineer field duo to long distance complex pipelines. Vibration analysis of pipeline can become prohibitive due to high computational time associated with large number of degrees of freedom. Thus, the efficient model reduction technique to analyze the vibration characteristics of pipeline system is challenging for designers. The objective of this paper is to propose a reduced order modeling method of parallel-pipeline with multi-supports. Considering the constraint interface stiffness at the elastic support and the coupled fluid-structure pipeline system, a novel reduced order model is obtained by assembling the reduced substructure element. Modal analysis is performed for parallel-pipeline system, and the calculation results for reduced order model are compared with the full finite element model. The obtained results indicate that the natural frequencies and mode shapes of the reduced order model are consistent with full finite element model. Moreover, an interesting phenomenon is obtained that the fluid velocity in one of parallel-pipeline can decrease the natural frequencies of another pipeline. The proposed model can obtain sufficient calculation accuracy and higher calculation efficiency, which can provide insight and guidance for engineers in aircraft.