Subsidence estimation of breakwater built on loosely deposited sandy seabed foundation: Elastic model or elasto-plastic model

Jianhua Shen,Huaicheng Wu,Yuting Zhang 대한조선학회 2017 International Journal of Naval Architecture and Oc Vol.9 No.4

In offshore area, newly deposited Quaternary loose seabed soils are widely distributed. There are a great number of offshore structures has been built on them in the past, or will be built on them in the future due to the fact that there would be no very dense seabed soil foundation could be chosen at planed sites sometimes. However, loosely deposited seabed foundation would bring great risk to the service ability of offshore structures after construction. Currently, the understanding on wave-induced liquefaction mechanism in loose seabed foundation has been greatly improved; however, the recognition on the consolidation characteristics and settlement estimation of loose seabed foundation under offshore structures is still limited. In this study, taking a semi-coupled numerical model FSSI-CAS 2D as the tool, the consolidation and settlement of loosely deposited sandy seabed foundation under an offshore breakwater is investigated. The advanced soil constitutive model Pastor- Zienkiewics Mark III (PZIII) is used to describe the quasi-static behavior of loose sandy seabed soil. The computational results show that PZIII model is capable of being used for settlement estimation problem of loosely deposited sandy seabed foundation. For loose sandy seabed foundation, elastic deformation is the dominant component in consolidation process. It is suggested that general elastic model is acceptable for subsidence estimation of offshore structures on loose seabed foundation; however, Young's modulus E must be dependent on the confining effective stress, rather than a constant in computation.

Subsidence estimation of breakwater built on loosely deposited sandy seabed foundation: Elastic model or elasto-plastic model

Shen, Jianhua,Wu, Huaicheng,Zhang, Yuting The Society of Naval Architects of Korea 2017 International Journal of Naval Architecture and Oc Vol.9 No.4

In offshore area, newly deposited Quaternary loose seabed soils are widely distributed. There are a great number of offshore structures has been built on them in the past, or will be built on them in the future due to the fact that there would be no very dense seabed soil foundation could be chosen at planed sites sometimes. However, loosely deposited seabed foundation would bring great risk to the service ability of offshore structures after construction. Currently, the understanding on wave-induced liquefaction mechanism in loose seabed foundation has been greatly improved; however, the recognition on the consolidation characteristics and settlement estimation of loose seabed foundation under offshore structures is still limited. In this study, taking a semi-coupled numerical model FSSI-CAS 2D as the tool, the consolidation and settlement of loosely deposited sandy seabed foundation under an offshore breakwater is investigated. The advanced soil constitutive model Pastor-Zienkiewics Mark III (PZIII) is used to describe the quasi-static behavior of loose sandy seabed soil. The computational results show that PZIII model is capable of being used for settlement estimation problem of loosely deposited sandy seabed foundation. For loose sandy seabed foundation, elastic deformation is the dominant component in consolidation process. It is suggested that general elastic model is acceptable for subsidence estimation of offshore structures on loose seabed foundation; however, Young's modulus E must be dependent on the confining effective stress, rather than a constant in computation.

Targeting RNA-splicing for SMA treatment

Zhou, Jianhua,Zheng, Xuexiu,Shen, Haihong Springer-Verlag 2012 Molecules and cells Vol.33 No.3

Predominant expression of exon 7 skipped SMN mRNAs in lung based on analysis of transcriptome sequencing datasets

Yang, Xiaoming,Shen, Haihong,Gao, Xue,Zheng, Xuexiu,Qin, Ryan,Zhou, Jianhua Springer-Verlag 2014 NEUROLOGICAL SCIENCES Vol.35 No.3

Chemical constituents from Viburnum fordiae Hance and their anti-inflammatory and antioxidant activities

Jia Chen,Jianhua Shao,Chunchao Zhao,Jie Shen,Ziling Dong,Wenyan Liu,Min Zhao,Judi Fan 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.6

Three new neolignans, fordianoles A-C (1–3),characterized as (7S,8R)-4-hydroxy-3,30,50-trimethoxy-80,90-dinor-8,40-oxyneolignan-7,70,9-triol, (7R,8R)-4-hydroxy-3,30,50-trimethoxy-80,90-dinor-8,40-oxyneolignan-7,70,9-triol, and (7R,8R)-4-hydroxy-3,30,50-trimethoxy-8,40-oxyneolignan-7,9,90-triol-70-one, together with an unusual c-lactone,3-(3,4-dihydroxyphenyl)-4-pentanolide (4), andtwenty-five known compounds (5–29) were isolated fromthe aerial parts of Viburnum fordiae Hance. Their structuresincluding absolute configurations were determined byspectroscopic and chemical methods. Among them, compounds6, 7, 11–15, 17–28 were isolated from the Viburnumgenus for the first time. The anti-inflammatory and antioxidantactivities of all compounds were evaluated in vitro. Compounds 15, 19, 20 and 29 showed significant inhibitoryactivity on NO production in RAW264.7 cells stimulated byLPS with IC50 values ranging from 8.60 to 13.92 lM. Meanwhile, compounds 1–4, 15, 19, 20, 22, 23, 25, 26 and29 exhibited varying antioxidant activities through DPPH,ABTS free radical scavenging and FRAP assays.

An Improved Anonymous Remote user Authentication Scheme with Key Agreement based on Dynamic Identity

Yajuan Shi,Han Shen,Yuanyuan Zhang,Jianhua Chen 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.5

To keep the pace with the development of internet technology, remote user authentication techniques become more and more important to protect user’s privacy. Recently, Kumari, et al., presented an improved remote user authentication scheme with key agreement based on dynamic-identity using smart card. This scheme allows legal users to change the password at his will without the need to connect the server. They claimed that their scheme could resist smart card stolen or loss attack, user impersonation and server masquerading attack, and provide user anonymity and untraceability and so on. However, our research indicates that their scheme is completely unsafe. Furthermore, the scheme can’t provide the proper mutual authentication. In this manuscript, we will propose a new scheme, which can withstand those attacks mentioned above and provide the perfect user anonymity and forward secrecy. Security analysis makes it clear that the improved scheme apparently is more secure and practical.

Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-${\kappa}B$ in Acute Transection Spinal Cord Injury in Rats

Shrestha, Rajiv Prasad,Qiao, Jian Min,Shen, Fu Guo,Bista, Krishna Bahadur,Zhao, Zhong Nan,Yang, Jianhua The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.56 No.5

Objective : To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) in spinal cord injury (SCI) in rats. Methods : BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-${\kappa}B$ in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Results : At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-${\kappa}B$ positive cells and level of NF-${\kappa}B$ messenger RNA in spinal cord at various time points between the groups. Activated NF-${\kappa}B$ immunoreactivity and level of NF-${\kappa}B$ mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05). Conclusion : BMMNCs transplantation attenuates the expression of NF-${\kappa}B$ in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.

Identification of novel splicing variants from RON proto-oncogene pre-mRNA.

Moon, Heegyum,Cho, Sunghee,Yang, Xiaoming,Zhou, Jianhua,Loh, Tiing Jen,Zheng, Xuexiu,Shen, Haihong National Hellenic Research Foundation 2012 ONCOLOGY REPORTS Vol.28 No.6

<P>RON is a proto-oncogene that induces cell dissociation, migration and matrix invasion. RON?160, a splicing variant of RON, is a natural splicing product in colon cancers that is produced through skipping of exons 5 and 6 in alternative splicing process. RON?160 promotes cellular transformation in?vitro and tumor formation in vivo. We present, here, two novel splicing variants of RON in the partial splicing events that involve exons 5 and 6. The common facts of these two novel splicing variants are that exons 4-7 are included. In addition, intron 4 is spliced whereas intron 5 is included in both variants. The difference of these two isoforms is the inclusion or skipping of intron 6. In one variant intron 6 is included, but intron 6 is skipped in another variant. These two variants should be truncated but these proteins have not yet been detected.</P>

Splicing inhibition of U2AF⁶⁵ leads to alternative exon skipping

Cho, Sunghee,Moon, Heegyum,Loh, Tiing Jen,Jang, Ha Na,Liu, Yongchao,Zhou, Jianhua,Ohn, Takbum,Zheng, Xuexiu,Shen, Haihong National Academy of Sciences 2015 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.112 No.32

<P><B>Significance</B></P><P>Transcription is a biological procedure in which DNA is transcribed to an RNA molecule. However, only fragments of this RNA are needed for protein synthesis. These fragments are exons that are interrupted by introns. Introns are removed by so-called RNA splicing process. Some exons could be alternatively included or excluded from the final RNA molecule. In this study, we have found that U2 snRNP auxiliary factor 65 kDa (U2AF<SUP>65</SUP>), a general splicing regulator, can significantly promote the exclusion of alternative exons. Strikingly, U2AF<SUP>65</SUP> suppresses flanking intron splicing of alternative exons, and even constitutive intron splicing. We deduce that the stimulatory effects of U2AF<SUP>65</SUP> on alternative exon exclusion are induced by the splicing inhibitory effects of U2AF<SUP>65</SUP>.</P><P>U2 snRNP auxiliary factor 65 kDa (U2AF<SUP>65</SUP>) is a general splicing factor that contacts polypyrimidine (Py) tract and promotes prespliceosome assembly. In this report, we show that U2AF<SUP>65</SUP> stimulates alternative exon skipping in spinal muscular atrophy (SMA)-related survival motor neuron (<I>SMN</I>) pre-mRNA. A stronger 5′ splice-site mutation of alternative exon abolishes the stimulatory effects of U2AF<SUP>65</SUP>. U2AF<SUP>65</SUP> overexpression promotes its own binding only on the weaker, not the stronger, Py tract. We further demonstrate that U2AF<SUP>65</SUP> inhibits splicing of flanking introns of alternative exon in both three-exon and two-exon contexts. Similar U2AF<SUP>65</SUP> effects were observed in Fas (Apo-1/CD95) pre-mRNA. Strikingly, we demonstrate that U2AF<SUP>65</SUP> even inhibits general splicing of adenovirus major late (Ad ML) or β-globin pre-mRNA. Thus, we conclude that U2AF<SUP>65</SUP> possesses a splicing Inhibitory function that leads to alternative exon skipping.</P>

Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer

Zhao Qiuyan,Zhu Zhonglin,Xiao Wenqin,Zong Guanzhao,Wang Chuanyang,Jiang Weiliang,Li Kai,Shen Jie,Guo Xingya,Cui Jianhua,Guo Lihong,Wan Rong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Pancreatic cancer (PC) is one of the most malignant tumors. Rapid progression and distant metastasis are the main causes of patient death. Hypoxia is a hallmark of multiple cancers and is involved in tumor biology. However, little is known about the roles of circRNAs in glycolysis and hypoxia-mediated progression of PC. Here, the expression pattern of hypoxia-related circRNAs was analyzed using RNA sequencing. A unique circRNA termed circRNF13 was found to be upregulated in PC tissues and may be a potential prognostic indicator. HIF-1α and EIF4A3 are involved in regulating the biogenesis of circRNF13. Furthermore, circRNF13 was validated to exert a stimulative effect on cell proliferation, angiogenesis, invasion and glycolysis. Importantly, we found that circRNF13 promoted PDK3 levels by acting as a miR-654-3p sponge, thus promoting the PC malignant process. Collectively, our results reveal that hypoxia-induced circRNF13 mediated by HIF-1α and EIF4A3 promotes tumor progression and glycolysis in PC, indicating the potential of circRNF13 as a prognostic biomarker and therapeutic target for PC.