Metastasis associated genomic aberrations in stage II rectal cancer

Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.

A Coumarin-based Fluorescent Sensor for Selective Detection of Copper (II)

Jian-Hong Wang,Xin-Ling Guo,Xu-Feng Hou,Hui-Jun Zhao,Zhao-Yang Luo,Jin Zhao 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.8

Cu (II) detection is of great importance owing to its significant function in various biological processes. In this report, we developed a novel coumarin-based chemosensor bearing the salicylaldimine unit (2) for Cu2+ selective detection. The results from fluorescence spectra demonstrated that the sensor could selectively recognize Cu2+ over other metal cations and the detection limit is as low as 0.2 μM. Moreover, the confocal fluorescence imaging in HepG2 cells illustrated its potential for biological applications.

ON OPIAL INEQUALITIES INVOLVING HIGHER ORDER DERIVATIVES

Zhao, Chang-Jian,Cheung, Wing-Sum Korean Mathematical Society 2012 대한수학회보 Vol.49 No.6

In the present paper we establish some new Opial-type inequalities involving higher order partial derivatives. The results in special cases yield some of the recent results on Opial's inequality and provide new estimates on inequalities of this type.

Incidence and Risk Factors of the Caudal Screw Loosening after Pelvic Fixation for Adult Spinal Deformity: A Systematic Review and Meta-analysis

Zhao Jian,Nie Zheng,Zhou Jiangjun,Liao Dongfa,Liu Da 대한척추외과학회 2024 Asian Spine Journal Vol.18 No.1

The purpose of this study was to assess the factors affecting caudal screw loosening after spinopelvic fixation for adult patients with spinal deformity. This meta-analysis calculated the weighted mean difference (WMD) and odds ratio (OR) using Review Manager ver. 5.3 (RevMan; Cochrane, London, UK). The loosening group was older than the control group (WMD, 2.17; 95% confidence interval [CI], 0.48–3.87; <i>p</i>=0.01). The S2 alar-iliac (S2AI) could prevent the caudal screw from loosening (OR, 0.43; 95% CI, 0.20–0.94; <i>p</i>=0.03). However, gender distribution (<i>p</i>=0.36), the number of fusion segments (<i>p</i>=0.24), rod breakage (<i>p</i>=0.97), T-score (<i>p</i>=0.10), and proximal junctional kyphosis (<i>p</i>=0.75) demonstrated no difference. Preoperatively, only pelvic incidence (PI) in the loosening group was higher (WMD, 5.08; 95% CI, 2.71–7.45; <i>p</i><0.01), while thoracic kyphosis (<i>p</i>=0.09), lumbar lordosis (LL) (<i>p</i>=0.69), pelvic tilt (PT) (<i>p</i>=0.31), pelvic incidence minus lumbar lordosis (PI–LL) (<i>p</i>=0.35), sagittal vertical axis (SVA) (<i>p</i>=0.27), and T1 pelvic angle (TPA) demonstrated no difference (<i>p</i>=0.10). PI–LL (WMD, 6.05; 95% CI, 0.96–11.14; <i>p</i>=0.02), PT (WMD, 4.12; 95% CI, 0.99–7.26; <i>p</i>=0.01), TPA (WMD, 4.72; 95% CI, 2.35–7.09; <i>p</i><0.01), and SVA (WMD, 13.35; 95% CI, 2.83–3.87; <i>p</i>=0.001) were higher in the screw loosening group immediately postoperatively. However, TK (<i>p</i>=0.24) and LL (<i>p</i>=0.44) demonstrated no difference. TPA (WMD, 8.38; 95% CI, 3.30–13.47; <i>p</i><0.01), PT (WMD, 6.01; 95% CI, 1.47–10.55; <i>p</i>=0.01), and SVA (WMD, 23.13; 95% CI, 12.06–34.21; <i>p</i><0.01) were higher in the screw loosening group at the final follow-up. However, PI–LL (<i>p</i>=0.17) demonstrated no significant difference. Elderly individuals were more susceptible to the caudal screw loosening, and the S2AI screw might better reduce the caudal screw loosening rate than the iliac screws. The lumbar lordosis and sagittal alignment should be reconstructed properly to prevent the caudal screw from loosening. Measures to block sagittal alignment deterioration could also prevent the caudal screw from loosening.

Experimental and Simulation Study of PEMFC based on Ammonia Decomposition Gas as Fuel

Zhao, Jian Feng,Liang, Yi Fan,Liang, Qian Chaos,Li, Meng Jie,Hu, Jin Yi The Korean Electrochemical Society 2022 Journal of electrochemical science and technology Vol.13 No.1

Compared with hydrogen, ammonia has the advantages of high gravimetric hydrogen densities (17.8 wt.%), ease of storage and transportation as a chemical hydrogen storage medium, while its application in small-scale on-site hydrogen production scenarios is limited by the need for complex separation equipment during high purity hydrogen production. Therefore, the study of PEMFC, which can directly utilize ammonia decomposition gas, can greatly expand the application of fuel cells. In this paper, the output characteristics, fuel efficiency and the variation trend of hydrogen concentration and local current density in the anode channel of fuel cell with the output voltage of PEMFC fueled by ammonia decomposition gas were studied by experiment and simulation. The results indicate that the maximum output power of the hybrid fuel decreases by 9.6% compared with that of the pure hydrogen fuel at the same inlet hydrogen equivalent. When the molar concentration of hydrogen in the anode channel is less than 0.12, the output characteristics of PEMFC will be seriously affected. Employing ammonia decomposition gas as fuel, the efficiency corresponding to the maximum output power of PEMFC is approximately 47%, which is 10% lower than the maximum efficiency of pure hydrogen.

ROOM-TEMPERATURE FERROMAGNETISM IN SnO 2 NANOFIBERS AND NANOTUBES PREPARED BY ELECTROSPINNING

JIAN-GUO ZHAO,WEI-YING ZHANG,ZHAO-JUN LIU,ZHONG-LI LIU,YA-JUAN ZHANG,ER-QING XIE,XIU-YUN AN,YONG-FENG CHEN,CHANG-YOU ZHANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.2

SnO 2 nano¯bers and nanotubes were synthesized by electrospinning method. Magnetizationmeasurement indicates that the SnO 2 nano¯bers and nanotubes annealed in air at 500?C exhibitthe room-temperature ferromagnetism and the ferromagnetism of nanotubes is stronger than thenano¯bers. Selected area electron di®raction, X-ray di®raction and Raman measurements showthat all the samples possess a typical rutile structure and no other impurity phases are observed. The results of the Raman spectra also indicate that there are lots of defects existing in thefabricated samples. The observed room-temperature ferromagnetism in SnO 2 nano¯bers andnanotubes possibly originates from oxygen vacancies. The ¯eld cooled (FC) and zero-¯eld-cooled(ZFC) magnetization curves indicate that the Curie temperature T C is above 300 K.

The Expression Characterization of Chicken Uncoupling Protein Gene

Zhao, Jian-Guo,Li, Hui,Wang, Yu-Xiang,Meng, He Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11

The UCPs are members of the mitochondrial inner membrane transporter family, present in the mitochondrial inner membrane. Their main function is increasing the energy expenditure via diminishing the resulting production of ATP from mitochondrial oxidative phosphorylation instead of yielding dissipative heat. They are associated with the metabolism of fat and regulation of energy expenditure. The UCP gene can be viewed as the candidate gene for chicken fatness. In the present study, RT-PCR and Northern Blot methods were developed to investigate the expression of the UCP gene in ten tissues including heart, liver, spleen, lung, kidney, gizzard, intestine, brain, breast muscle and abdominal fat of chicken. The results of both RT-PCR and Northern Blot methods showed that the UCP gene expressed specific in breast muscle. The expression levels of UCP gene in breast muscles from egg-type and meat-type chickens of hatching, 2, 4, 6 and 8 wk of age were detected by RT-PCR assay and results showed that the expression levels of UCP gene were related to breeds. Expression level of UCP gene in layers was higher than that in broilers at various weeks of age except at 6 wk. The UCP gene's expression was higher at 6 wk and had no significant difference among other weeks of age in broilers; in layers the expression level of UCP gene had no significant difference among weeks of age. The experiment results also showed that insulin could increase the expression level of UCP gene by 40% compared with control group.

A Design Approach for the Interior Anchorage Zone of Post-tensioned Concrete Structure

Jian-Li Zhao,Shui-Long Shen,Lin-Bing Wang,Jun Chen 대한토목학회 2011 KSCE Journal of Civil Engineering Vol.15 No.3

For the interior anchorage zone in post-tensioned concrete structure, International Federation for Presterssing (FIP) proposed a design method based on the theory of Strut-and-Tie Model (STM) (called FIP-STM in the following context). However, the condition of FIP-STM is that the dimension ratio of anchor, b/h, is equal to 1/9. In this paper, the stress distribution in interior anchorage zone was analyzed via using Finite Element Method (FEM) at first. The relationship between ratio of internal force over post-tensioned load (F/P) and dimension ratio (b/h) (F/P-b/h curve) was obtained. FEM results show that in case of b/h<1/9, the interior anchorage zone can be simplified to the core region, in which b/h is equal to 1/9 and FIP-STM is still applicable. However, in case of b/h>1/9, the amount of non-prestressed rebar designed in interior anchorage zone by use of FIP-STM is more than that required, and in some cases it is difficult to allocate non-prestressed rebar in the crowded space. Based on the results of stress analysis, a modified approach for FIP-STM was presented, which can be applied for any dimension ratio. With the proposed modification on FIP-STM, the non-prestressed rebar can be reasonably allocated in the narrow space of interior anchorage zone via modifying the internal forces and geometry of FIP-STM. The analytical result shows that for interior anchorage zone about 30% of non-prestressed rebar can be reduced via using the proposed method compared with that using FIP-STM if b/h is equal to 3/10.

Preferential association of a functional variant in complement receptor 2 with antibodies to double-stranded DNA

Zhao, Jian,Giles, Brendan M,Taylor, Rhonda L,Yette, Gabriel A,Lough, Kara M,Ng, Han Leng,Abraham, Lawrence J,Wu, Hui,Kelly, Jennifer A,Glenn, Stuart B,Adler, Adam J,Williams, Adrienne H,Comeau, Mary E H. K. Lewis 2016 Annals of the rheumatic diseases Vol.75 No.1

<P><B>Objectives</B></P><P>Systemic lupus erythematosus (SLE; OMIM 152700) is characterised by the production of antibodies to nuclear antigens. We previously identified variants in complement receptor 2 (<I>CR2/CD21</I>) that were associated with decreased risk of SLE. This study aimed to identify the causal variant for this association.</P><P><B>Methods</B></P><P>Genotyped and imputed genetic variants spanning <I>CR2</I> were assessed for association with SLE in 15 750 case-control subjects from four ancestral groups. Allele-specific functional effects of associated variants were determined using quantitative real-time PCR, quantitative flow cytometry, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP)-PCR.</P><P><B>Results</B></P><P>The strongest association signal was detected at rs1876453 in intron 1 of <I>CR2</I> (p<SUB>meta</SUB>=4.2×10<SUP>−4</SUP>, OR 0.85), specifically when subjects were stratified based on the presence of dsDNA autoantibodies (case-control p<SUB>meta</SUB>=7.6×10<SUP>−7</SUP>, OR 0.71; case-only p<SUB>meta</SUB>=1.9×10<SUP>−4</SUP>, OR 0.75). Although allele-specific effects on B cell <I>CR2</I> mRNA or protein levels were not identified, levels of complement receptor 1 (<I>CR1/CD35)</I> mRNA and protein were significantly higher on B cells of subjects harbouring the minor allele (p=0.0248 and p=0.0006, respectively). The minor allele altered the formation of several DNA protein complexes by EMSA, including one containing CCCTC-binding factor (CTCF), an effect that was confirmed by ChIP-PCR.</P><P><B>Conclusions</B></P><P>These data suggest that rs1876453 in <I>CR2</I> has long-range effects on gene regulation that decrease susceptibility to lupus. Since the minor allele at rs1876453 is preferentially associated with reduced risk of the highly specific dsDNA autoantibodies that are present in preclinical, active and severe lupus, understanding its mechanisms will have important therapeutic implications.</P>

A SANDWICH-INJECTION METHOD FOR MICROCHIP ELECTROPHORESIS

JIAN-LONG ZHAO,GANG LI,GUI-SHENG ZHUANG,HONG-BO ZHOU,YUAN-SEN XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2007 NANO Vol.2 No.6

In microchip electrophoresis (μ-CE), sample injection is generally achieved through cross, double-T, or T-form injector structures. In these reported approaches, the separation efficiency and detection sensitivity of μ-CE is significantly influenced by the shape and size of the sample plug introduced into the separation channel or sample leakage in separation phase. Here, we present a sandwich-injection method for controlling discrete sample injection in μ-CE. This method involves four accessory arm channels in which symmetrical potentials are loaded to form a unique parallel electric field distribution at the intersection of sample and separation channels. The parallel electric field effectuate a virtual wall to confine the shape of a sample plug and depress the spreading of the sample plug at the junction of sample and separation channels, and also prevent sample leakage during separation step. The key features of this method are the ability to inject well-defined sample plugs at the original sample concentration and the ability to control the sample plug size. The virtues of the novel injection technique were demonstrated with numerical models and validated with fluorescence visualizations of electrophoretic experiments.