대식세포 NAD glycohydrolase에 관한 연구

이지영,안년형 圓光大學校 1995 論文集 Vol.30 No.2

NAD glycohydrolase (NADase:EC 3.2.2.5)는 포유류에서 형질막에 glycosylphosphatidylinositol (GPI) 결합으로 연결되어 있어 박테리아의 phosphatidylinositol-특이성 phospholipase C (PI-PLC)로 가용화 된다. 본 연구에서는 결핵 감염된 마우스로 부터 대식세포를 얻어 LPS처리후 막외 NADase가 유리되는 것을 관찰하였다. 이 활성화된 대식세포에서 NADase가 유리되기에 앞서서 대식세포에 내재하는 GPI-PLC 활성이 증가하는 사실을 관찰하였다. 이는 대식세포의 활성화에 의해 대식세포에 내재하는 GPI-PLC활성이 증가하므로써 GPI결합으로 붙어 있는 NADase가 유리됨을 의미한다. 활성화 대식세포에서 증가된 GPI-PLC 활성 분획을 DEAE column 크로마토그라피를 시행하여 단일 peak의 용출액을 얻은 후 이분획에서 활성을 확인한 결과 GPI-PLC 활성은 없음을 알았다. NAD glycohydrolase (NADase) is located on the surface of the cell via glycosylphosphatidylinositol (GPI)-linkage, which can be cleaved by bacterial PI-specific phospholipase C (PI-PLC). When macrophages primed with live or dead Mycobacterium tuberculosis were treated with liposaccharide, ecto-NADase was released from activated macrophages. The result that the release of NADase followed the increase of GPI-PLC activity in macrophages suggests that endogenous GPI-PLC can cleave the PI-linkage of NADase on the macrophages. The GPI-cleavae activity was shown as a single peak on DEAE chromatography and the activity was not inhibited by phenanthroline, a selective inhibitor of GPI-phospholipase D (GPI-PLD). There data indicate that the release of NADase from activated macrophages may be due to the activity of GPI-PLC, but not GPI-PLD.

모노드라마의 빛과 그림자 : 솔로구프와 예브레이노프의 극장 The Theatres of Fedor Sologub and Nikolai Evreinov театр Федора Сологуба и театр НИkолая Евреинова

차지원 서울대학교 러시아연구소 2006 러시아연구 Vol.16 No.1

Потребность 'общения' между сценическим представлением и зрителем предопределяет исходную эстетическую ориентацию модернистского театра в начале ХХ века в России. Влекуемаяся в проблеме соотношения сцены и зрительного зала идея 'общения' в театре, выражаясь в разных сценических опытах модернизма, поднимается как общепринятая эстетическая задача модернистского театра. Первой попыткой решения 'общения' в театре является театр 'мистерии' и 'соборный театр', понятие которых развилось у Вячеслава Иванова. При этом парадоксально что, стремление соборного театра совершить 'общение' междусценой и зрителем в театре до своего предела, т.е. его стремление достигнуть абсолютного уровня возможности 'общения' театрального сообщения обрекает 'соборный театр' на эстетический гибель. В 'соборном театре' 'абсолютное' 'общение' между сценой и зрителем падает в свою очередь в 'нулевое'. Эстетически крайную концепцию 'абсолютного' 'общения' и монологизма наследует театр монодрамы в модернистском театре, в том числе сугубо субъективный театр Федора Сологуба и экспериментальный театр Николая Евреинова. В театре Ф. Сологуба, идея монодрамы, соприкасаясь с концепцией абсолютной субъективности творческой личности, обосновывает пессимическое эстетическое мировозрение автора о творчестве как 'высокое безумие', эстетическое 'бегство' от грубой действительности. Над 'абсолютным' 'общением' в театреСологуба висят тени небытия. В связи с этим театр Н. Евреинова кажется как бы освещенным ярким лучом жизни. Монодрама Евреинова, достигнутая абсолютным общением между сценойи зрителей, пользуется как стратегия 'театрализации' жизни, которая является эстетическим лозунгом театр Евреинова. Оба театра, кажущиеся тем, что противоположно развивают идею монодрамы в краях пессимизма и оптимизма, на самом деле интергрируется к одному и тому же эстетическому тупику 'монологизма'. Оба они приводятся к 'мимикрии', в которой призыв к 'общению' поворачивается его отрицанием. Театр Сологба, в котором прячется идея эстетического бегства от действительности, который отрицает всякую возможность бытия в реальном мире, и театральная игра как 'simulation', ложное сознание иллюзии, которое смещает жизненную реальность в театре Н. Евреинова, все же рождают 'пустые' знаки, 'sigпifiапt'ы пустотыи небытия. Возможность 'общения' в театре существует ни в концепции 'абсолютного'совершенного 'общения' между сценой и зрителем, ни в монодраме, а именно в диалогизме и множественности контекстов, в полифонизме и многоголосьи, которые монологизм напрасно стремлится снимать со сцены.

이지오더 신사복에 관한 소비자 선호도 및 구매의사 조사

천종숙,허지혜,송지영 복식문화학회 2001 服飾文化硏究 Vol.9 No.3

The purpose of this study is to examine the growth potentiality of the Easy-Order System in the domestic male business suit market. This study is based on a questionnaire survey of males, who usually wear a business suit in the work place, and females whose husband wear a business suit. The pertinently answered 108 questionnaires (males: 72 and females: 36) were analyzed with SPSS 8.0. The results of this survey were as follows; 1.Gender, occupation, and height of consumers had a considerable effect on their intention of purchasing a business suit through the Easy-Order System. Average monthly earnings of consumers affected their preference of this system. 2.Subjects, who were dissatisfied with the limited option of purchasing a business suit in retail shops, had a significant intention of purchasing a business suit through the Easy-Order System. 3.It had no effect on consumers' preference and purchasing intention of the Easy-Order System whether or not they recognize this system. And the Easy-Order System is not estimated to have major repercussions over consumers by now. Key words: Easy-Order(이지오더), business suit(신사복), consumer's preference(소비자 선호도), purchasing intention(구매의사).

A Stereochemical Aspect of Pyridoxal 5'-Phosphate Dependent Enzyme Reactions and Molecular Evolution

JHEE, KWANG-HWAN,YOSHIMURA, TOHRU,KUROKAWA, YOICHI,ESAKI, NOBUYOSHI,SODA, KENJI 한국미생물 · 생명공학회 1999 Journal of microbiology and biotechnology Vol.9 No.6

We have studied the stereospecificities of various pyridoxal 5'-phosphate (PLP) dependent enzymes for the hydrogen transfer between the C-4' of a bound coenzyme and the C-2 of a substrate in the transamination catalyzed by the enzymes. Stereospecificities reflect the structures of enzyme active-sites, in particular the geometrical relationship between the coenzyme-substrate Schiff base and the active site base participating in an α-hydrogen abstraction. The PLP enzymes studied so far catalyze only a si-face specific (pro-S) hydrogen transfer. This stereochemical finding suggests that the PLP enzymes have the same topological active-site structures, and that the PLP enzymes have evolved divergently from a common ancestral protein. However, we found that D-amino acid aminotransferase, branched chain L.-amino acid aminotransferase, and 4-amino-4-deoxychorismate lyase, which have significant sequence homology with one another, catalyze a re-face specific (pro-R) hydrogen transfer. We also showed that PLP-dependent amino acid racemases, which have no sequence homology with any aminotransferases, catalyze a non-stereospecific hydrogen transfer: the hydrogen transfer occurs on both faces of the planar intermediate. Crystallographical studies have shown that the catalytic base is situated on the re-face of the C-4' of the bound coenzyme in D-amino acid aminotransferase and branched chain L-amino acid aminotransferase, whereas the catalytic base is situated on the si-face in other aminotransferases (such as L-aspartate aminotransferase) catalyzing the si-face hydrogen transfer. Thus, we have clarified the stereospecificities of PLP enzymes in relation with the primary structures and three-dimensional structures of the enzymes. The characteristic stereospecificities of these enzymes for the hydrogen transfer suggest the convergent evolution of PLP enzymes.

Biotechnology of Aflatoxins

Jhee, Eun-Chung INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1989 YONSEI REPORTS ON TROPICAL MEDICINE Vol.20 No.1

Aflatoxins which is produced by the fungus, asperfillus flavus was widely distributed in the human food supply like cones, peanuts and grains. It has been wellknown to be a potent toxin causing acute aflatoxicosis liver cancer. As informations on aflatoxin problems accumulated, it has become apparent that the risk of exposure to aflatoxins is much less in technologically developed countries than in developing ones. The role of the hepattitis B virus and aflatoxin B₁in the developmetn of primary hepatocellular carcinoma (PHC) was studied in southeast Asia and Africa. Using aflatoxin B₁binding to DAN as an indicator of hepatocarcinogenic potency, the effect of antioxidant was examined to see how this agent modulate the hepatocarcino-genesis of aflatoxins in rats. We also find the mechanism of this inhibition by antioxidant is through the modulation of cytosolic detoxifying enzyme, glutathione s-transferases.

Effect of CA and Si on the Microstructure and Aging Characteristics of AZ91 Alloy

Jhee, Tae-Gu Research Institute for Science & Technology, Hong- 2000 Hongik Journal of Science and Technology Vol.4 No.-

The effects of Ca and Si on the microstructures and aging characteristics were investigated in the T5 treatment of AZ91 magnesium alloys rapidly solidified in vacuum inside a stainless steel pipe. The addition of Ca(0.88%), Si(0.25%) to the AZ91 alloy reduced the dendrite cell size with the effect of Si being more effective. Si in AZ91 alloy formed Mg₂Si compound during the T5 treatment at dendrite cell boundaries as well as in the matrix and this compound decreased the precipitation rate. Ca in AZ91 alloy did not form any compound with other alloying elements and was segregated mainly around the secondary phase such as Mg_(12)Al_(7), and some of Ca formed a ternary eutectic (Mg-Al-Ca) structure. This alloy had a good precipitation property and high temperature strength. In the Ca+Si added AZ91 alloys, the element Ca promoted the homogeneous distribution of fine Mg₂Si compound. The AZ91 alloys containing Ca+Si , therefore, have much finer microstructures than those containing either Ca or Si. So AZ91 alloys containing Ca+Si were more effective at decreasing the precipitation rate and had a superior high temperature tensile strength to AZ91, AZ91+Ca and AZ91+Si alloys.

A flatoxin B_1 carcinogenesis and its prevention by Butylated hydroxyanisole

Jhee, Eun-Chung 全北大學校 齒醫學硏究所 1985 전북치대논문집 Vol.3 No.1

흰쥐에 butylated hydroxyanisole (BHA)를 전처치 한후 강력한 간암 유발제인 aflatoxin과 흰쥐간의 DNA와의 결합능의 정도를 생체내 실험은 물론 시험관내 실험에서도 비교관찰하였다. 대조군과 BHA로 전처치한 흰주의 간을 대상으로 aflatoxin-B_1 DNA 결합능을 비교해보면 cytochrome P450 함량이나 microsome에 연관된 aflatoxin B_1-DNA양에는 별차이가 없었다. cytosol의 저해효과는 대조군의 2배에 비하여 BHA전처치때는 5배나 저해 시키므로서 현저하게 저해 작용이 증진 됨을 알수 있었다. 이와같은 aflatoxin B_1-DNA결합능에 미치는 cytosol의 저해작용은 송아지 흉선의 exogenous DNA와 신선한 흰쥐의 간세포에서 직접 분리한 endogenous DNA에서 모두 비슷한 양상을 나타냈다. 1mM 농도의 trichloropropene oxide나 styrene oxide는 aflatoxin B_1-DNA결합능에 대한 cytosol의 저해 작용을 부분적으로 다시 환원 시킬뿐아니라 흰쥐를 희생시키기 2시간 전에 aflatoxin B_1을 복강내로 주사 한후 DNA와의 결합능을 관찰해 보면 대조군에서 보다 BHA로 전처치한 실험군에서 50%나 AF B_1-DNA 결합능이 감소되는 것을 볼 수 있었다. 이상의 결과로 미루어 볼 때 BHA로 전처치한 흰쥐에서는 GSHS-transferase가 유되되어 간의 Aflatoxin B_1-DNA 결합능을 조절하는 것이 시사되었다.

DSS Architectures to Support Data Mining Activities for Supply Chain Management

Jhee, Won-Chul,Lee, Hyun-Chan,Suh, Min-Soo Research Institute for Science & Technology, Hong- 2000 Hongik Journal of Science and Technology Vol.4 No.-

Application potentials of data mining are evaluated in the various areas of supply chain management (SCM), and architectures of decision support systems (DSS) that support the data mining activities are suggested. We first briefly introduce data mining and review the recent literatures on SCM and then evaluate data mining applications to SCM in three aspects: marketing, operations management and information systems. By analyzing the cases about pricing models in distribution channels, demand forecasting and quality control, it is shown that artificial intelligence techniques such as artificial neural networks, case-based reasoning and expert systems, combined with traditional analysis models, effectively mine the useful knowledge from the large volume of SCM data. Agent-based information system is addressed as an important architecture that enables us to pursue the global optimization of SCM through communication and information sharing among supply chain constituents without loss of their characteristics and independence. We expect that the suggested architectures of intelligent DSS provide us with the basis in developing information systems for SCM to improve the quality of organizational decisions.

Inhibition of Microsome-mediated Binding of Aflatoxin B1 to DNA by Glutathione S-transferase

Jhee, Eun Chung,Prabhaker D. Lotlikar 전북대학교 의과학연구소 1981 全北醫大論文集 Vol.5 No.1

Time Series Modeling Using Backpropagation Network: ARMA Model Identification

Jhee,Won-Chul 弘益大學校 1990 弘大論叢 Vol.22 No.2

경영예측을 위한 시계열 모형들 중에서 Autoregressive Moving Average(ARMA) 모형은 이론적 우수성가 예측의 정확성에도 불구하고, 적절한 ARMA모형의 선정이 어려워 실제로 많이 이용되지 못하고 있다. 본 연구에서는 적은 시간 내에 많은 종류의 시계열로부터 ARMA모형을 이용하여 자동적으로 예측치를 얻기 위해, 통계적 방법론과 신경망(Artificial Neural Network, ANN)의 효과적인 결합을 모색한다. ARMA 모형선정의 어려움을 해결하기 위해, 이를 패턴분류문제로 생각하여, 패턴분류기로써 신경망을 사용하고, 특성추출기로써 확장된 표본자기상관함수(Extended Sampl Autocorr elation Function, ESACF)를 사용하였다. 사용된 신경망 모형은 Backpropagation 네트웍이며, ESACF에 대한 전처리와 네트웍의 분리 등을 고려한 세 가지 신경망 설계 대안이 검토되었다. 실험결과는 신경망이 ARMA 모형의 선정에 효과적으로 사용될 수 있음을 보여준다.