The effects of antibiotics on the reproductive physiology targeting ovaries in the Asian tiger mosquito, Aedes albopictus

Ha Young‐Ran,Jeong Se‐Jin,Jang Chang‐Won,Chang Kyu‐Sik,Kim Hyun‐Woo,Cho Shin‐Hyoung,Lee Hee‐Il 한국곤충학회 2021 Entomological Research Vol.51 No.2

Mosquitoes have adapted to various environmental conditions. Symbionts with mosquitoes impact this adaptation in different environments. In the field, mosquitoes could get exposed to antibiotics during their developmental period, which could reduce or eliminate their symbiotic microbes. However, the side effects of the antibiotics on the ovary and reproductive physiology of the Asian tiger mosquito, Aedes albopictus remains unknown. In this study, we investigated the effects of tetracycline and combinations of rifampicin and tetracycline at environmentally acceptable levels on the reproductive physiology of ovaries in Ae. albopictus. Rifampicin and tetracycline in combination reduced the hatching rate and fertility of Ae. albopictus compared to the untreated control group. These antibiotics induced histopathological damage and reactive oxygen species production in the ovaries. The combination of antibiotics decreased the expression of surface protein of Wolbachia (WSP) in Ae. albopictus. Additionally, the expression of Toll like receptor 2 (TLR2) and Myd88 were triggered by the combinations. The findings demonstrate the detrimental effects of antibiotics, particularly combinations of rifampicin and tetracycline, on the reproductive capacity of Ae. albopictus females.

Soil CO₂ efflux in a warm-temperature and sub-alpine forest in Jeju, South Korea

Jeong, Heon-Mo,Jang, Rae-Ha,Kim, Hae-Ran,You, Young-Han The Ecological Society of Korea 2017 Journal of Ecology and Environment Vol.41 No.6

Background: This study investigated the temporal variation in soil $CO_2$ efflux and its relationship with soil temperature and precipitation in the Quercus glauca and Abies koreana forests in Jeju Island, South Korea, from August 2010 to December 2012. Q. glauca and A. koreana forests are typical vegetation of warm-temperate evergreen forest zone and sub-alpine coniferous forest zone, respectively, in Jeju island. Results: The mean soil $CO_2$ efflux of Q. glauca forest was $0.7g\;CO_2\;m^{-2}\;h^{-1}$ at $14.3^{\circ}C$ and that of A. koreana forest was $0.4g\;CO_2\;m^{-2}\;h^{-1}$ at $6.8^{\circ}C$. The cumulative annual soil $CO_2$ efflux of Q. glauca and A. koreana forests was 54.2 and $34.2t\;CO_2\;ha^{-1}$, respectively. Total accumulated soil carbon efflux in Q. glauca and A. koreana forests was 29.5 and $18.7t\;C\;ha^{-1}$ for 2 years, respectively. The relationship between soil $CO_2$ efflux and soil temperate at 10 cm depth was highly significant in the Q. glauca ($r^2=0.853$) and A. koreana forests ($r^2=0.842$). Soil temperature was the main controlling factor over $CO_2$ efflux during most of the study period. Also, precipitation may affect soil $CO_2$ efflux that appeared to be an important factor controlling the efflux rate. Conclusions: Soil $CO_2$ efflux was affected by soil temperature as the dominant control and moisture as the limiting factor. The difference of soil $CO_2$ efflux between of Q. glauca and A. koreana forests was induced by soil temperature to altitude and regional precipitation.

남산의 소나무림, 신갈나무림, 아까시나무림의 낙엽생산과 토양유기탄소 동태

정헌모 ( Heon Mo Jeong ),김해란 ( Hae Ran Kim ),신동훈 ( Dong Hoon Shin ),이경미 ( Kyoung Mi Lee ),이승혁 ( Seung Hyuk Lee ),한영섭 ( Young Sub Han ),장래하 ( Rae Ha Jang ),이상경 ( Sang Kyeong Lee ),김태규 ( Tae Kyu Kim ),유영한 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.2

남산의 소나무림, 신갈나무림 그리고 아까시나무림에서 2008년 1월부터 2009년 12월까지의 월별 낙엽생산량과 토양의 유기탄소량을 조사하였다. 소나무림은 2008년과 2009년 모두 10월에 낙엽생산량이 가장 많았으며, 2008년 1월과 2009년 12월에 낙엽생산량이 가장 적었다. 신갈나무림은 2008년과 2009년 모두 11월에 낙엽생산량이 가장 많았으며, 2월에 낙엽생산량이 가장 적었다. 아까시나무림은 2008년 11월과 2009년 10월에 낙엽생산량이 가장 많았으며, 2008년 1월과 2009년 12월에 낙엽생산량이 가장 적었다. 이것은 한반도 중부에서 상록성의 소나무림이 낙엽성의 참나무숲 보다 더 일찍 낙엽이 진다는 것을 의미한다. 소나무림과 신갈나무림, 아까시나무림의 2년 평균의 낙엽생산량은 7.07, 6.36, 5.02 ton ha-1로 소나무가 가장 많았다. 소나무림과 신갈나무림, 아까시나무림의 2년 평균의 토양유기탄소량은 76.2, 68.6, 72.5 ton C ha-1로 소나무림에서 가장 많았다. 이처럼 소나무숲에서 높게 나타난 것은 남산에서 신갈나무나 아까시나무를 벌목하여 임목밀도가 줄었기 때문이다. The objective of this study was to estimate dynamics of litter production and soil organic carbon of Pinus densiflora forest, Quercus mongolica forest, and Rhobina pseudo-acasia forest at Mt. Nam as a part of Korea National Long-Term Ecological Research (KNLTER) from 2008 to 2009. Litter production of P. densiflora forest was the highest in October 2008, 2009 and the lowest in January 2008 and December 2009. Litter production of Q. mongolica forest was the highest in November and the lowest in February in 2008 and 2009. Litter production of R. pseudo-acacia forest was the highest in November in 2008 and October in 2009 and the lowest in January in 2008 and December in 2009. It means that leaves of P. densiflora forest shed earlier than deciduous oak forests in Korean central region. An average of litter production for 2 years was 7.07, 6.36, 4.66 ton ha-1 in P. densiflora forest, Q. mongolica forest, R. psuedo-acacia forest, respectively. An average of soil organic carbon matter for 2 years was 88.3, 76.5, 84.2 ton ha-1 in P. densiflora forest, Q. mongolica forest, R. psuedo-acacia forest, respectively.

Case Report : A Case of Microscopic Polyangiitis: Glomerulonephritis, Neuropsychiatric Abnormalities, and Urticarial Vasculitis

( Ran Hui Cha ),( Ha Jeong Lee ),( Soo Hee Kim ),( Eun Jung Jung ),( Kyung Chul Moon ),( Yon Su Kim ) 대한신장학회 2010 Kidney Research and Clinical Practice Vol.29 No.1

We report a case of microscopic polyangiitis, presenting with rapidly progressive glomerulonephritis, neuropsychiatric abnormalities, and urticarial vasculitis. A 65-year-old woman reported loss of appetite, significant weight loss, and a transient history of veering tendency. She was presented with a mild fever, cough, and sputum. Routine laboratory test revealed anemia, leukocytosis with a left shift, azotemia, and elevated highly sensitive C-reactive protein. The bilateral kidneys were observed to be enlarged (right kidney 16.3 cm, left kidney 18.2 cm) on an abdominal computed tomography. The perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA) titer was >1:640 and MPO-ANCA was positive. Her chief complaints consisted of character change, visual illusion, and hearing loss. A skin rash with a bullous change resembling urticaria was further developed. Kidney biopsy demonstrated pauci-immune diffuse crescentic glomerulonephritis. Skin biopsy was compatible with urticarial vasculitis. Having received intravenous cyclophosphamide and oral prednisolone, she showed sustained improvement in renal function, as well as her neuropsychiatric symptoms and skin rash.

An analysis of cognitive demands of mathematical tasks in Korea and Japan elementary school textbooks in proportional reasoning

Ha-Na Park,Jeong-Yee Han,Hae-Rin Yoon,Soo-Yeon Jeong,Doo-Ran Kim,Kwang-Ho Lee 한국교원대학교 뇌기반교육연구소 2017 Brain, Digital, & Learning Vol.7 No.1

The purpose of this study was to examine and analyze the cognitive demand of the mathematical tasks suggested in the elementary school textbooks in Korea and Japan. In particular, it aimed to reveal the overall picture of the level of cognitive demand of the mathematical tasks in the strand of proportional reasoning in the textbooks. We adopted the framework for mathematical task analysis suggested by Stein & Smith(1998) and analyzed the mathematical tasks accordingly. The findings from the analysis showed that 38.9 percent of the mathematical tasks were at high level and the rest at low level in terms of cognitive demand in Korea. In Japan 18.7 percent of the mathematical tasks were at high level and the rest at low level in terms of cognitive demand. Most of the mathematical tasks in the textbooks were algorithmic and focused on producing correct answers by using procedures in both countries.

Determination of Optimal Growing Degree Days and Cultivars of Kimchi Cabbage for Growth and Yield during Spring Cultivation under Shading Conditions

Ha Seon Sim,Won Jun Jo,Hye Jin Lee,Yu Hyun Moon,Ui Jeong Woo,Soo Bin Jung,Su Ran Ahn,Sung Kyeom Kim 한국원예학회 2021 원예과학기술지 Vol.39 No.6

This study determined the optimal growing degree days (GDD) and cultivars of Kimchi cabbage (Brassica rapa L. ssp. pekinensis) for growth and yield during spring cultivation under shading condition. There were nine treatments, and the experiments were conducted with three GDD levels (899, 924, and 961) and three cultivars (‘Chungwang’, ‘Cheongna’, and ‘Cheongok’) under shading conditions. The process-based model of Kimchi cabbage was applied for growth and yield prediction using meteorological data regarding light, temperature, wind, precipitation, and humidity in several regions. The number of leaves of ‘Chungwang’ Kimchi cabbage was greater than that of ‘Cheongna’ and ‘Choengok’ at all planting times. Most of the growth parameters were highest in the delayed transplanting treatment than in the other transplanting times. ‘Chungwang’ Kimchi cabbage was less sensitive to high temperature during spring cultivation than the other cultivars. GDD 924 was the most optimal time to harvest under the shading condition. GDD 750-870 is considered an appropriate harvest time in the primary production regions during the spring cultivation season. If shading is performed in these regions, constant production may be possible, thus, alleviating damage from high temperature.

Effects of Mulerian Inhibiting Substance in human endometrial stromal cells

( Jeong Namkung ),( Youn Jee Chung ),( Jae Eun Ha ),( Hyun Hee Jo ),( Mee Ran Kim ),( Young Ok Lew ),( Jang Heub Kim ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

Clinical implications of healthcare-associated infection in patients with community-onset acute pyelonephritis

Ha, Young Eun,Kang, Cheol-In,Joo, Eun-Jeong,Park, So Yeon,Kang, Seung Ji,Wi, Yu Mi,Chung, Doo Ryeon,Peck, Kyong Ran,Lee, Nam Yong,Song, Jae-Hoon Informa Healthcare 2011 Scandinavian journal of infectious diseases Vol.43 No.8

<P><I>Background</I>: Clinical and microbiological characteristics of healthcare-associated acute pyelonephritis (HCA-APN) have not been described in detail yet. We sought to delineate the differences between community-associated (CA)- and HCA-APN with specific interest in antibiotic resistance of causative microorganisms. <I>Methods</I>: We conducted a retrospective cohort study during a 1-y period at a large referral center. Patients who visited the emergency department with symptoms and signs of APN were included in the study population. <I>Results</I>: Among 319 cases with community-onset APN, 201 cases (63%) were classified as HCA-APN. Patients with HCA-APN had higher SOFA (sequential organ failure assessment) scores, longer length of hospital stay and a lower rate of complete response to antimicrobial therapy. Patients with complicated APN also had characteristics similar to those seen in HCA-APN. However, 14-day mortality rates were not different between CA-APN vs HCA-APN and between uncomplicated APN vs complicated APN. With regard to microbiological characteristics, Escherichia coli were less common in HCA-APN than in CA-APN (62.7% vs 93.2%, <I>p</I> < 0.001). Among E. coli isolates, quinolone resistance and extended-spectrum beta-lactamase (ESBL) production were more common in HCA-APN than in CA-APN (38.9% vs 12.7%, <I>p</I> < 0.001; 15.9% vs 0.8%, <I>p</I> < 0.001, respectively). <I>Conclusions</I>: HCA-APN, and complicated APN, represents a distinct subset of urinary tract infections with more antibiotic-resistant pathogens and worse outcomes, which physicians should consider to provide optimal treatment.</P>

Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric detection

Jeong, Ji-Seon,Kwon, Ha-Jeong,Lee, Yong-Moon,Yoon, Hye-Ran,Hong, Seon-Pyo Elsevier 2007 Journal of Chromatography A Vol.1164 No.1-2

<P><B>Abstract</B></P><P>We have developed an improved analytical method for the determination of sugar phosphates using sodium carbonate (Na<SUB>2</SUB>CO<SUB>3</SUB>) for high-performance anion-exchange chromatography–pulsed amperometric detection. The target analytes were separated completely within 10min using eluent containing 20mM NaOH and 35mM Na<SUB>2</SUB>CO<SUB>3</SUB>. The limit of detection (S/N=3) and quantitation (S/N=10) for analytes were 10–30ng/mL and 35–100ng/mL, respectively. Linear dynamic range was 1–30μg/mL (<I>r</I><SUP>2</SUP>≥0.9998). The RSDs for intra- and inter-day assays were found to be of satisfactory results (0.23–3.09%), and the recoveries from blood spots were 97.62–99.69%.</P>

Effect of Chitosan Foliar Application on Soluble Sugars, Glucosinolates, and ABA catabolites Contents in ‘Chungwang’ Kimchi cabbage under Extreme and Moderate High Temperature

Ha Seon Sim,Ui Jeong Woo,Won Jun Jo,Hye Jin Lee,Yu Hyun Moon,Soo Bin Jung,Su Ran Ahn,Jung Su Jo,Sung Kyeom Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

Physiological disorder (calcium deficiency) of Kimchi cabbage occurred by high temperature and frequent precipitation, which leads to unexpected price fluctuations of commodities. This purpose of this study was to profile on soluble sugars, glucosinolates, and ABA catabolites contents of Kimchi cabbage leaves under extreme and moderate high temperature treatments and chitosan foliar application. At April 6, 2020, the ‘Chungwang’ Kimchi cabbage were transplanted into 625L plastic boxes filled with commercial and general soil at open-field in Wanju. Experiments were combined with three day/night temperature levels, 36/32°C (E; extreme high), 28/24°C (M; moderate high), and 20/16°C (C; control), and two chitosan foliar application (200g/L; and non chitosan foliar application). Temperature and chitosan foliar application were performed at 42 days after transplanting, and temperature treatments were maintained during 8 days in extreme weather growth simulators. The samples were collected in 4 and 8 days after commencing high temperature treatments. After 4 days of chitosan foliar application, the glucose and fructose contents of E treatment were 67.5 and 59.4 μg·g<SUP>-1</SUP>, respectively, which were higher than non application. In the chitosan foliar application treatment, the aliphatic (gluconapin and glucobrassicanapin) and indole (glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) glucosinolate contents of E treatment were 151.1, 342.2, 533.1, 1627, and 1807 μg·g<SUP>-1</SUP>, respectively, which were greater than non application. The ABA, DPA, and PA contents of E treatment under chitosan foliar application were 0.0114, 1.7, and 1.0 μg·g<SUP>-1</SUP>, respectively, higher than non application. After 8 days of chitosan folir application, the gluconapin, glucobrassicin, 4-methoxyglucobrassicin, and DPA contents of E treatment were 332.0, 445.8, 2037, 2.4 μg·g<SUP>-1</SUP>, respectively, which were greater than non application. There were no effects of chitosan foliar application in M and C treatments. Results indicated that the effect of chitosan foliar application is estimated to be approximately 4 days after commencing high tempearture treatment. The application of chitosan foliar increased ABA catabolites that relieve abiotic stress, it is considered to be effective in alleviating the high temperature damage of Kimchi cabbage.