치수/치근단 질환에 이환된 영구치의 치수 조직 재생과 치근 형성

유연지,백승호,손호현 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.4

최근 치수 질환 또는 치근단 질환을 가진 미성숙 영구치에 대한 보존적 치료의 방법으로 여러가지 근관 내 소독 약제를 이용하에 증상 개선은 물론 치근의 성장 및 치수의 재생이 이루어진 증례들이 보고되고 있다. 그 기전에 대해서는 아직 명확하게 밝혀지지는 않았지만 여러가지 줄기 세포 또는 미분화 간엽 세포들이 관여하는 것으로 생각되며, 실제로 재생된 조직에서는 대부분백악질양또는골양물질의 침착이 관찰되고 있다. 이 새롭고보존적인 치료접근 방법은 다능성 줄기 세포와 다양한조직 공학 기술에 대한 연구와 더불어, 재생적 근관 치료에 더 밝은 비전을 제시하고 있다. Numerous cases about additional growth of roots or pulp tissue regeneration by using various intracanal medicaments in immature permanent teeth with periapical or pulpal disease have been reported. The underlying mechanism has not been clearly delineated. but it has been widely accepted that undifferentiated mesenchymal cells and stem cells are involved. Moreover, the growth and deposition of osteoid or cementoid tissues have been observed in regenerated pulp and roots. This new and non-invasive treatment has brightened the future of endodontics. and enlarged the vision of regenerative root canal treatment with multi-potent stem cells and various tissue engineering techniques.

Complete Mucleotide Sequence and Organization of the Mitogenome of the Red-spotted Apollo Butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) and Comparison with other Lepidopteran Insects

Man Il Kim,Jee Yeon Baek,Min Jee Kim,Heon Cheon Jeong,Ki Gyoung Kim,Yeon Soo Han,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

The 15,389-bp long complete mitogenome of the endangered red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, which have the gene order of tRNAMet, tRNAIle, and tRNAGln at the beginning. Due to the uncertainty the start codon for COI gene in insect has been discussed extensively. We propose the CGA sequence as the start codon for COI gene in lepidopteran insects, based on complete mitogenome sequences of lepidopteran insects including our P. bremerii and additional sequences of the COI start region from a diverse taxonomic range of lepidopteran species (a total of 51 species belonging to 15 families). As has been suggested in other sequenced lepidopteran insects the 18 bp-long poly-T stretch and the downstream conserved motif ATAGA that were previously suggested to serve as a structural signal for minor-strand mtDNA replication also was found at the 3’-end region of the P. bremerii A+T-rich region. In an extensive search to find out tRNA-like structure in the A+T-rich region, each one tRNATrp-like sequence and tRNALeu (UUR)-like sequence were found in the P. bremeri A+T-rich region, and most of other sequenced lepidopteran insects were shown to have tRNA-like structure within the A+T-rich region, thereby indicating that such feature is frequent in the lepidopteran A+T-rich region. Phylogenetic analysis using the concatenated 13 amino acid sequences and nucleotide sequences of PCGs of the four macrolepidopteran suferfamilies together with Tortricoidea and Pyraloidea well recovered a monophyly of Papilionoidea and a monophyly of Bombycoidea. However, Geometroidea and Noctuoidea were unexpectedly clustered as one group and placed this group to the sister group to Bombycoidea, instead of Papilionoidea in most analyses.

Sequence Analysis of the Start Region of Lepidopteran Mitochondrial COI Gene

Jee Yeon Baek,Man Il Kim,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Invertebrate mitochondrial genome contains 13 protein-coding genes and major start codons for them are ATA (Met) and ATG (Met). However, alternative start codons such as ATT (Ile), ATC (Ile), TTG (Leu), and GTG (Val) also have been suggested from a diverse organism. Approximately 120 complete mitochondrial genome reported showed that the start codon for COI gene evidences an array of diverse designation of COI start codon such as typical ATN, tetranucleotide TTAG and ATAA, newly proposed AAT and AAC and so on. In the case of Lepidoptera, many completely sequenced species showed no typical start codon at the start context of COI and even within the neighboring tRNATyr. In order to clarify, we newly sequenced the beginning context of COI gene, encompassing the neighboring tRNATyr and start region of COI gene from 39 species belonging to eight lepidopteran families. We found the newly sequenced 39 species and 14 available complete lepidopteran mitochondrial genomes all possessed CGA (arginine), which is the first non-overlapping in-frame codon in COI gene. Furthermore, this CGA is highly well aligned in terms of both nucleotide and amin o acid sequences with neighboring region. Thus, the CGA (arginine) may be synapomorphic character for Lepidoptera, functionally constrained. We, therefore, propose the CGA sequence as the start codon for COI gene in lepidopteran insects.

미술치료를 위한 섬유매체의 활용

백지민(Baek, Jee-Min), 박재연(Park, Jae-Yeon) 조선대학교 조형미술연구소 2012 조형미술논문집 Vol.12 No.2

현재 정신적인 분야가 많이 넓어지고 있는 시점에서 미술이라는 도구를 사용한 상담치료인 미술치료가 발전하고 있다. 한국은 미술치료가 시행된 지 얼마 되지 않아, 기반이 잘 다져지지 않았지만 다양한 형태로 발전하고 있는 과도기적인 시기를 겪고 있다. 이에 따라 미술치료에 사용되는 매체의 중요성을 짚어주고 치료에 사용되는 매체가 여러 모습으로 활발하게 발전되길 바라는 마음으로 매체중의 하나인 섬유매체를 통해 연구하고자 하였다. 섬유매체는 예부터 사람과 땔 수 없는 매체중의 하나로 치료에 활용되고는 있지만 어떻게 하면 내담자와의 소통에 더욱 활용될 수 있을까 하는 생각을 해 보았다. 목장갑, 휴대용주머니, 헌 옷, 부직포를 이용해 이미 사용된 섬유매체는 다른 방향으로 또 사용되지 않았던 섬유매체는 색다르게 프로그램의 하나로 연구해 보았다. 이를 통해 내담자가 미술이라는 매개체에 흥미를 잃지 않고 더욱 적극적으로 자신을 표현하기 바라며 앞으로 매체의 다양한 발전이 미술치료 분야에서 더욱 깊이 있게 발전하길 원하는 바이다. Keywords : 미술치료, 섬유매체 Art Therapy, Fiber Media

Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on <i>Candida albicans</i>–infected Root Dentin

Yoo, Yeon-Jee,Kwon, Ikyung,Oh, So-Ram,Perinpanayagam, Hiran,Lim, Sang-Min,Ahn, Ki-Bum,Lee, Yoon,Han, Seung-Hyun,Chang, Seok-Woo,Baek, Seung-Ho,Zhu, Qiang,Kum, Kee-Yeon Elsevier 2017 JOURNAL OF ENDODONTICS - Vol.43 No.11

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in <I>Candida albicans</I>–infected human root dentin.</P> <P><B>Methods</B></P> <P>Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with <I>C. albicans</I> for 3 weeks. They were randomly divided into 4 groups (<I>n</I> = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically.</P> <P><B>Results</B></P> <P>All medicaments showed significantly lower CFU than saline (<I>P</I> < .05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (<I>P</I> > .05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (<I>P</I> > .05), and both medicaments had significantly lower CFU than CH at both depths (<I>P</I> < .05).</P> <P><B>Conclusions</B></P> <P>In this <I>ex vivo</I> model of <I>C. albicans</I>–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Candida albicans</I> biofilm is closely related to initiation and persistence of refractory apical periodontitis. </LI> <LI> Antifungal efficacy of various intracanal medicaments were assessed in <I>C. albicans</I>-infected human root dentin. </LI> <LI> In <I>C. albicans</I>-infected human dentin blocks, root canals were covered by mature biofilms with tubular penetration of 400 μm. </LI> <LI> In this standardized <I>ex vivo</I> model, chlorhexidine and synthetic human β-defensin-3-C15 peptide were similar and highly effective at reducing <I>C. albicans</I> CFU, whereas calcium hydroxide had limited efficacy. </LI> </UL> </P>

Geographic Homogeneity and High Gene Flow of the Pear Psylla, Cacopsylla pyricola Foerster (Homoptera: Psyllidae), Detected by Mitochondrial COI Gene and Nuclear Ribosomal Internal Transcribed Sequence 2

Ah Rang Kang,Jee Yeon Baek,Sang Hyun Lee,Young Sik Cho,Wol Soo Kim,Yeon Soo Han,Iksoo Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

The pear psylla, Cacopsylla pyricola Foerster (Homoptera: Psyllidae), is a serious insect pest of commercial pear crops. The species, which resides on pear trees throughout its life cycle, is rapidly spreading in some regions of the world. Given the life cycle, it is unclear how such a rapid spread has been facilitated. Presently, the population genetic structure of the species including genetic diversity and gene flow was studied to understand the nature of dispersal and field ecology of the species. Pear psylla was collected from several pear orchards in Korea. The 658-bp region of mitochondrial COI gene and the 716-bp long complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA were sequenced. Unlikely other previously studied insect pests, the COI-based genetic diversity of the pear psylla was extremely low (maximum sequence divergence of 0.15%). This finding allowed us to conclude that the species may have been introduced in Korea relatively recently, possibly with the phenomenon of genetic bottlenecks. ITS2 sequence-based analyses of phylogeny, population differentiation, gene flow, and hierarchical population structure all concordantly suggested that the pear psylla populations in Korea are neither genetically isolated nor hampered for gene flow. These genetic data are concordant with the dispersal of an overwintering winterform morph outside the non-pear habitat in the fall and the possibility of subsequently longer distant dispersal.

How Well Do Current Climate Models Simulate Impacts of Arctic Warming to Extra Tropical Climate?

Yeon-Soo Jang,Jong-Seong Kug,Jee-Hoon Jeong,Baek-Min Kim 한국기상학회 2015 한국기상학회 학술대회 논문집 Vol.2015 No.10

골수소견상 전골수세포의 증가를 동반한 Idiopathic Hypereosinophilic Syndrome 1예

김연선,안지영,김휘준,김순길,신승호,백승호,김창진 大韓血液學會 1992 大韓血液學會誌 Vol.27 No.2

Uncertain Association Between Benzodiazepine Use and the Risk of Dementia: A Cohort Study

Baek, Yeon-Hee,Lee, Hyesung,Kim, Woo Jung,Chung, Jee-Eun,Pratt, Nicole,Kalisch Ellett, Lisa,Shin, Ju-Young Elsevier Science B. V., Amsterdam 2020 Journal of the American Medical Directors Associat Vol.21 No.2

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>To examine the association between benzodiazepine use and the risk of dementia.</P> <P><B>Design, setting, and participants</B></P> <P>We conducted a retrospective cohort study, using a nationwide healthcare database of South Korea (2002–2016). The participants included new users of benzodiazepines aged ≥50 years, with no prior prescription record of benzodiazepines or a history of dementia within the previous 5 years (2002–2006).</P> <P><B>Methods</B></P> <P>Outcome was defined as an incident dementia with specified algorithms using diagnosis and prescription records, with the application of a 5-year lag-time following the index date during which outcomes were censored. We used a multivariable Cox proportional hazard model to estimate hazard ratio (HR) and the 95% confidence interval (CI). Comorbidities and comedications were treated as time-varying covariates in 90-day windows, and an active comparator was used to reduce potential bias from confounding by indication. Active comparators were defined as new-users of antidepressants.</P> <P><B>Results</B></P> <P>Our final participants included 616,256 patients, after propensity score estimation and matching on a 1:1 ratio. We observed a 23% increase in the risk of dementia in benzodiazepine users, compared with that in nonusers, over a mean follow-up period of 5.5 years (HR 1.23, 95% CI 1.14–1.32). A consistent finding was observed when the lag-time duration was extended to 7 years, revealing a close to null association (HR 1.17, 95% CI 1.04–1.30). When new-users of antidepressants were used as the active comparator, no increase in the risk of dementia with benzodiazepines was observed over 7 years (HR 1.01, 95% CI 0.81–1.27).</P> <P><B>Conclusions and implications</B></P> <P>A significant association was observed between benzodiazepine use and the risk of dementia, compared with nonusers. However, a null or negative association was observed with the use of the active comparator, suggesting the absence of a causal association between dementia and benzodiazepine use.</P>

Impact of Arctic warming on extratropical climate variation in CMIP5

Yeon-Soo Jang,Jee-Hoon Jeong,Baek-Min Kim,Jong-Seong Kug 한국기상학회 2013 한국기상학회 학술대회 논문집 Vol.2013 No.10