Autotaxin stimulates urokinase-type plasminogen activator expression through phosphoinositide 3-kinase–Akt–necrosis factor kappa B signaling cascade in human melanoma cells

Lee, Jangsoon,Duk Jung, In,Gyo Park, Chang,Han, Jeung-Whan,Young Lee, Hoi Lippincott Williams Wilkins, Inc. 2006 Melanoma research Vol.16 No.5

Autotaxin, a lysophospholipase D producing lysophosphatidic acid, augments invasive and metastatic potential of tumor cells. Current investigations have focused on understanding the molecular mechanisms by which autotaxin regulates the expression of a major mediator of tumor invasion and metastasis, urokinase-type plasminogen activator (uPA) in human A2058 melanoma cells. Autotaxin induced uPA expression in a dose-dependent manner that was inhibited by pharmacological inhibitors for Gi (pertussis toxin), phosphoinositide 3-kinase (PI3K, LY294002), Akt inhibitor (AktI), proteosome activity and I&kgr;B phosphorylation (pyrrolidine dithiocarbamate), and by a dominant negative mutant (DN) of Akt. Autotaxin phosphorylated Akt and induced the translocation of necrosis factor-&kgr;B (NF-&kgr;B) to the nucleus that were inhibited by AktI or by overexpressing DN-Akt. Consistently, green fluorescence protein-tagged p65 of NF-&kgr;B accumulated in the nucleus by autotaxin that was abrogated when the cells were transfected with DN-Akt. Moreover, autotaxin increased the DNA binding ability of NF-&kgr;B and promoter activity of uPA. Collectively, these data strongly suggest autotaxin induces uPA expression via the Gi–PI3K–Akt–NF-&kgr;B signaling pathway that might be critical for autotaxin-induced tumor cell invasion and metastasis.

An Empirical Study on the Effect of a High Performance Work System on the Human Resource Performance of Accounting Experts in Korean Companies: Focusing on the Mediated Effect of Informal Learning

Yongbo Shim(Yongbo Shim),Taehyoung Mun(Taehyoung Mun),Jangsoon Lee(Jangsoon Lee) The International Academy of Global Business and T 2023 The International Academy of Global Business and T Vol.19 No.4

Purpose – The purpose of this study is to verify the effect of a high-performance work system on human resource performance and the mediating effect of informal learning in this causal relationship. Design/Methodology/Approach – The research method of this study is a quantitative approach, and the data was aggregated to the 1st Human Capital Corporate Panel Survey II (HCCPS) by the Korea Research Institute for Vocational Education & Training (KRIVET) due to its focus on the experience of accounting experts in Korean companies. A total of 1,024 participants were included in this study. Findings – The study found that high-involvement work organization and high-commitment HRM had a positive effect on organizational commitment and job competence in Korean companies, and that informal learning had a partial mediating effect in the causal relationship between a high-involvement work organization and organizational commitment and job competence. Research Implications – From a theoretical perspective, this research highlights the importance of informal learning in linking high-performance work system with human resource performance. In addition, this study also demonstrates the relational impact of high-commitment HRM and high-involvement work organization in Korean companies, further expanding the literature on workplace performance. From a practical perspective, this study suggests that companies need to incorporate effective high performance work systems, and build an environment to enable active informal learning opportunities. In addition, institutions should promote learning initiatives to maximize human resource effectiveness, ultimately improving organizational performance.

Electrical performance of MFCs in lake sediment

Jangsoon LIM,Seongmin LEE,Kyuwon SEO,Jungsu PARK,Heehoon JUNG,Jongho NOH,Hyung Joo KIM 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.4

유기성 바인더 촉매를 활용한 슬러지 고형화 및 에너지원으로의 재활용

이종선(Chongsun Lee),김조영(Joyoung Kim),정요셉(Joseph Jeong),박장순(Jangsoon Park) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11

회계전문인력의 일터학습 실태와 참여촉진요인에 대한 실증연구

심용보(Yongbo Shim),이장순(Jangsoon Lee) 한국경영교육학회 2018 한국경영교육학회 학술발표대회논문집 Vol.2018 No.11

[연구목적] 본 연구는 회계전문인력이 일터학습 프로그램에 적극적으로 참여하게 되는 영향요인들을 탐색하여 이 요인들과 일터학습의 활성화간의 인과관계를 분석하고자 하였다. [연구방법] 본 연구에서는 위의 목적을 달성하기 위해 2015년 한국직업능력개발원의 6차 인적자본기업패널조사의 통계자료에서 656명 회계전문인력의 응답치를 선택하여 통계적 검증을 하였다. 가설검증을 위한 통계방법으로는 학습의 자기결정성, 과업 다양성, 경영진의 학습지향 리더십이 형식학습과 무형식학습의 활성화에 미치는 영향을 다중회귀분석을 통해 분석하였다. [연구결과] 분석결과. 회계전문인력들은 형식학습에서는 집체식 사내교육에, 무형식학습에서는 OJT에 가장 많은 참여율을 보였다. 반면 회계전문인력들은 형식학습 총 5개 중에서 평균 1.84개의 프로그램에 참여했고 무형식학습 총 5개 중에서는 평균 0.66개의 프로그램에 참여하였다. 가설검증결과를 보면 독립변수인 학습의 자기결정성, 과업 다양성, 경영진의 학습지향 리더십 모두는 형식학습과 무형식학습에 정(+)적 영향을 미치는 것으로 나타났다. 또한 일터학습에서 고학습 집단이 학습성과변수인 직무능력, 조직몰입, 직무만족, 전반적인 신뢰정도, 인적자원역량 경쟁력에 있어 가장 높은 점수를 보였고 저학습집단은 가장 낮은 점수를 보였다. [연구의 시사점] 본 연구는 한국기업의 회계전문인력을 대상으로 형식학습뿐만 아니라 무형식학습의 실태를 조사했고 나아가 일터학습의 참여촉진요인과 일터학습의 활성화간의 인과관계를 실증했다는 점에서 이론적 의의가 있다고 하겠다. [Purpose] The purpose of this study is to research the impact of characteristics on accounting expert’s participation of workplace learning in Korean companies. [Methodology] The data in this study were collected from 656 accounting experts in 10,069 samples workers which were extracted from Korean companies. [Findings] The results of empirical analyses indicated that the accounting experts participated in an average of 1.84 programs out of 5 types of formal learning, and participated in an average of 0.66 programs out of the total 5 informal learning programs and the promoting characteristics(the manager’s learing support, task variety, self-determination) had the positive effects on the activation of workplace learning. [Implications] The high-learning groups had have a higher score on learning performances(Job capability, Organizational commitment, Job satisfaction, Trust , Human resource competence than the low-learning groups.

Quantitative analysis of glucose from hazardous plants in Korea for industrial feedstock

Jungsu PARK,Yongkeun CHOI,Seongmin LEE,Hyunjin JEON,Jangsoon LIM,Young-Hoo KIM,Heehun JUNG,Sang Hyun LEE,Yung-Hun YANG,Hyung Seop KIM,Hyung Joo KIM 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

수압파쇄법을 이용한 ○○ 원유비축시설 내 수벽 터널에서의 초기응력 측정

배성호(Seongho Bae),김재민(Jaemin Kim),김장순(Jangsoon Kim),이영호(Youngho Lee) 한국암반공학회 2008 터널과지하공간 Vol.18 No.1

암반 내 작용하는 초기응력이 지하 암반 구조물의 안정성에 미치는 영향은 시공 심도가 깊어지고 단면규모가 커질수록 증가하게 된다. 일반적으로 초기응력 측정은 암반 구조물의 설계단계에서 시공 예상지역 내지표 수직공을 이용하여 수압파쇄법에 의해 이루어져 왔다. 그러나 조사단계에서 초기응력 측정이 이루어지지 않았거나 지형, 지질 특성상 과지압의 가능성이 있거나 또는 조사 과정에서 높은 수준의 초기응력장 분포가 확인된 지역에서는 시공의 안정성과 기존 설계의 적합성을 확인하기 위해서는 시공 현장의 지하 공간에서 상세초기응력 측정을 수행하는 것이 바람직하다. 본 논문의 연구지역은 전라남도 OO시 남동부에 위치한 추가 석유 비축기지 건설현장으로 설계 전 상세 지반조사를 통해 측압계수 3.0 이상의 값을 가지는 과잉 수평응력장이 관찰된 지역이다. 본 연구에서는 비축기지 지하 건설 현장 내 굴착된 수벽터널용 공동에서 시추된 2개의 시험공에서 수압파쇄법에 의한 초기응력 측정을 시행하였다. 조사 범위는 지표로부터 약 180 m ~ 300 m 심도구간이며 균열 조사에는 초음파 주사검층법을 적용하였다. 지표로부터 심도 200 m 이상인 일부 조사 구간에서 측압계수 2.50 이상의 값을 가지는 큰 수평응력 성분들이 관찰되었고 전반적인 시험 결과는 설계 전 단계에서 수행된 조사 결과와 매우 유사한 양상을 나타내었다. The influence of in-situ rock stress on the stability of an underground rock structure increases as the construction depth become deeper and the scale of a rock structure become larger. In general, hydraulic fracturing stress measurement has been performed in the surface boreholes of the target area at the design stage of an underground structure. However, for some areas where the high horizontal stresses were observed or where the overstressed conditions caused by topographical and geological factors are expected, it is desirable to conduct additional in-situ stress measurement in the underground construction site to obtain more detailed stress information for ensuring the stability of a rock structure and the propriety of current design. The study area was a construction site for the additional underground oil storage facility located in the south-east part of OO city, Jeollanam-do. Previous detailed site investigation prior to the design of underground structures revealed that the excessive horizontal stress field with the horizontal stress ratio(K) greater than 3.0 was observed in the construction area. In this study, a total of 13 hydraulic fracturing stress measurements was conducted in two boreholes drilled from the two water tunnel sites in the study area. The investigation zone was from 180 m to 300 m in depth from the surface and all of the fracture tracing works were carried out by acoustic televiewer scanning. For some testing intervals at more than 200 m in depth from surface, the high horizontal stress components with the horizontal stress ratio(K) greater than 2.50 were observed. And the overall investigation results showed a good agreement with the previously performed test.

Ethanolic extract of Melia azedarach L. induces melanogenesis through the cAMP-PKA-CREB signaling pathway

김미옥,박세정,See-Hyoung Park,Sae Woong Oh,Seung Eun Lee,Ju Ah Yoo,Kitae Kwon,Jangsoon Kim,Min Hee Kim,Jae Youl Cho,Jongsung Lee 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.1

Backgrounds: Since the cause of hypopigmentary skin disorders (hair graying and vitiligo) is typically unknown, there is no known cure for these disorders. Melia azedarach L. is used in Southeast Asia across China and Japan as a traditional medicine, and it has been reported to have various pharmacological properties. However, there have been no reports to demonstrate the involvement of M. azedarach L. in pigmentation. This study was conducted to investigate the effect of ethanolic extract of M. azedarach L. (MAE) on melanogenesis and to elucidate its mechanism of action in B16F10 mouse melanoma cells and human epidermal melanocytes. Methods: Effects of MAE on melanogenesis and its mechanism of action were investigated using several assays, including melanin content, cellular tyrosinase activity, real-time PCR analysis, Western blot analysis, and ELISAs for cyclic AMP (cAMP), protein kinase A (PKA), cAMP response element binding (CREB) protein, and mitogen-activated protein kinases (MAPKs). Results: MAE increased the melanin content levels and cellular tyrosinase activity in B16F10 mouse melanoma cells and human epidermal melanocytes. In addition, the action mechanism of MAE-induced melanogenesis was examined in human epidermal melanocytes. It also upregulated the expressions of microphthalmia-associated transcription factor (MITF) gene and its downstream target genes, tyrosinase and tyrosinase-related protein (TRP) 1, but not TRP 2. MAE treatment increased the cAMP levels, PKA activity, and phosphorylation of CREB protein, its downstream signaling protein. However, MAE showed no effects on MAPKs (p42/ 44 MAPK, p38 MAPK, and c-Jun-N-terminal kinase (JNK)). Conclusion: These findings indicate that MAE induces melanogenesis by upregulating the MITF gene through the cAMP-PKA-CREB signaling pathway, and they suggest its potential in the treatment of hypopigmentary skin diseases.

Norepinephrine induces VEGF expression and angiogenesis by a hypoxia‐inducible factor‐1α protein‐dependent mechanism

Park, Soon Young,Kang, Joo Hee,Jeong, Kang Jin,Lee, Jangsoon,Han, Jeong Whan,Choi, Wahn Soo,Kim, Yong Kee,Kang, Jaeku,Park, Chang Gyo,Lee, Hoi Young Wiley Subscription Services, Inc., A Wiley Company 2011 International journal of cancer: Journal internati Vol.128 No.10

<P><B>Abstract</B></P><P>A growing number of studies have demonstrated that physiological factors can influence the progression of several cancers <I>via</I> cellular immune function, angiogenesis and metastasis. Recently, stress‐induced catecholamines have been shown to increase the expression of various cancer progressive factors, including vascular endothelial growth factor (VEGF), matrix metalloproteinases and interleukins. However, a detailed mechanism remains to be identified. In this study, we investigated the role of adrenergic receptors and hypoxia‐inducible factor (HIF)‐1α protein in catecholamine‐induced VEGF expression and angiogenesis. Treatment of the cells with norepinephrine (NE) or isoproterenol induced VEGF expression and HIF‐1α protein amount in a dose‐dependent manner. Induction of VEGF expression by NE was abrogated when the cells were transfected with HIF‐1α–specific siRNA. Similarly, adenylate cyclase activator forskolin and cyclic AMP‐dependent protein kinase A inhibitor H‐89 enhanced and decreased HIF‐1α protein amount, respectively. More importantly, conditioned medium of NE‐stimulated cancer cells induced angiogenesis in a HIF‐1α protein–dependent manner. In addition, pretreatment of cells with propranolol, a β‐adrenergic receptor (AR) blocker, completely abolished induction of VEGF expression and HIF‐1α protein amount by NE in all of the tested cancer cells. However, treatment with the α1‐AR blocker prazosin inhibited NE‐induced HIF‐1α protein amount and angiogenesis in SK‐Hep1 and PC‐3 but not MDA‐MB‐231 cells. Collectively, our results suggest that ARs and HIF‐1α protein have critical roles in NE‐induced VEGF expression in cancer cells, leading to stimulation of angiogenesis. These findings will help to understand the mechanism of cancer progression by stress‐induced catecholamines and design therapeutic strategies for cancer angiogenesis.</P>

Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1

Kwon, Kitae,Park, See-Hyoung,Han, Byung Seok,Oh, Sae Woong,Lee, Seung Eun,Yoo, Ju Ah,Park, Se Jung,Kim, Jangsoon,Kim, Ji Woong,Cho, Jae Youl,Lee, Jongsung MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.9

<P>Urban particulate matter (UPM) exerts negative effects on various human organs. Transient receptor potential vanilloid 1 (TRPV1) is a polymodal sensory transducer that can be activated by multiple noxious stimuli. This study aimed to explore the effects of the UPM 1648a on the expression of TRPV1, and its regulatory mechanisms in HaCaT cells. UPM enhanced TRPV 1 promoter-luciferase reporter activity. UPM also increased expression of the TRPV 1 gene as evidenced by increased mRNA and protein levels of TRPV 1. In addition, elucidation of the underlying mechanism behind the UPM-mediated effects on TRPV 1 expression revealed that UPM can upregulate expression of the TRPV1 gene by activating activator protein-1 (AP-1) and nuclear factor kappa B (NF-κB). The UPM treatment also altered Ca<SUP>2+</SUP> influx and cell proliferation, as well as production of interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β). In addition, these UPM-induced effects were attenuated by SB203580 and ammonium pyrrolidinedithiocarbamate (PDTC). However, SP600125 and PD98059 did not alter the UPM-induced effects. Taken together, these findings indicate that UPM upregulates expression of the TRPV 1 gene, which is mediated by the p38 mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways and suggest that UPM is a potential irritant that can induce skin processes such as aging and inflammatory responses.</P>