[PG-0007] Identification and Determination of Phenolic compounds in Rapeseed (Brassica napus L.) Mutant Lines Derived from Gamma-ray

Jaihyunk Ryu(Jaihyunk Ryu ),Baul Yang(Baul Yang ),Ye-Jin Lee(Ye-Jin Lee ),Dong-Gun Kim(Dong-Gun Kim ),Sang Hoon Kim(Sang Hoon Kim ),Sang-Il Han(Sang-Il Han ),Bo-Keun Ha(Bo-Keun Ha ),Si-Yong Kang(Si-Yo 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Assessment of Growth and Seed Oil Composition of Kenaf (Hibiscus cannabinus L.) Germplasm

Jaihyunk Ryu,강시용,하보근,김동섭,김진백,Sang Hoon Kim 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4

This study was conducted to evaluate the growth characteristics and fatty acid composition among 15 kenaf mutants derived fromthe kenaf germplasm C14 and 15 kenaf accessions originating from Russia, India, China, Iran, and Italy. The overall growth performance(plant height, stem diameter, flowering date, leaf, and flower size) of the stem color mutant lines derived from C14 are similarto those of the original variety. However, the flower color mutant lines derived from C14 showed flowering to occur 10 days laterwhen compared with the original variety and showed smaller leaf sizes than the original variety. Late-ripened kenaf accessions(Jinju, Auxu, and Jnagdae) can yield more bio-mass compared with early or medium-maturing germplasm. The late maturity kenaf(Auxu, Jinju, and Jangdae) has a higher oil percentage than the early maturity germplasm. Linoleic, oleic, and palmitic acids were thepredominant fatty acids in all kenaf seeds. The stem color mutant lines significantly surpassed the parental means of all saturatedfatty acids. In addition, the flower color mutant lines showed broad ranges of variation in oleic acid. The 15 accessions showed awide range of fatty acid compositions, spanning from 29.75 to 38.30% saturated fatty acids and 61.70 to 70.24% total unsaturatedfatty acids, and the late maturity kenaf has a higher linoleic acid percentage than the early maturity germplasm. The flowering periodwas highly positively (P ² 0.01) correlated with the plant height, stem diameter, oil percent, and linolenic acid (C18:3), and it was significantlynegatively (P ² 0.01) correlated with stearic acid (C18:0). These results will provide valuable information to assist theparental selection of kenaf breeding.

Chemical Components in the Leaves of Selected Mutant Cultivars of kenaf (Hibiscus cannabinus L.)

Jaihyunk Ryu,Sang-Wook Jeong,Seung Bin Im,Joon-Woo Ahn,Soon-Jae Kwon,Dong Sub Kim,Jin-Baek Kim,Sang Hoon Kim,Si-Yong Kang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Kenaf (Hibiscus cannabinus L.) native to Africa can be used as fiber, food, feedstock and bio plastic. This study was carried out to evaluate the mineral, amino acid and vitamin contents of six selected kenaf cultivars which are enable to produce seed under Korean circumstance. The leaves of three mutant cultivars (Jangdae, Jeokbong and Baekma), two original cultivars (Jinju, C14) and one Chinese cultivar (Auxu) were harvested at flowering time. Mineral components of kenaf leaves, such as calcium, potassium, and mineral, did not showed significant differences among the cultivars. As major amino acids including proline and phenylalanine, significant differences were found in these kenaf cultivars. The Auxu cultivar contained the highest amount of essential amino acid (Phenylalanine, Leucine, Isoleucine, Valine, Methionine and Lysine). The amount of vitamin displayed significant differences such as vitamin E and vitamin K among these cultivars. Especially, Jangdae cultivar contained the highest amount of vitamin E and vitamin K. Thus, these data suggested that Jangdae and Auxu is the most desirable cultivar containing high amount of vitamin and amino acid.

Effect of Electron Beam on in vitro Cultured Orchid (Cymbidium) Organs

Jaihyunk Ryu,Hyun-Su So,Hyun Suk Kang,Byung Cheol Lee,Chang-Hyu Bae 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Ionizing radiations have been effective mutagen sources to overcome the limitation of the useful genetic resources in natural environment. The study was conducted to investigate an effect of electron beam on organogenesis, growth patterns and genetic variation in the irradiated orchid organs (rhizome and protocorm). The in vitro cultured rhizomes of orchids (Cymbidium goeringii, C. kanran) were irradiated with the electron beam in the dose range of 15Gy to 2240Gy under the condition of various beam energy and beam current (10MeV/n and 0.5mA, 10MeV/n and 0.05mA, 2MeV/n and 0.5mA). Significant decreases in growth and organogenesis were observed by increase of intensity of electron beam irradiation. The irradiation intensity of lethal dose 50 (LD50) of the in vitro cultured orchid was estimated as approximately 500Gy to 1000Gy under 10MeV/n, and 1000Gy to 2000Gy under 2MeV/n. While, the optimal irradiation dose was approximately 100Gy for growth and organogenesis of the cultures under 10MeV/n with 0.05mA treatment, and 300Gy under 2MeV/n and 0.5mA electron beam condition. RAPD and ISSR analyses for the electron beam irradiated organs were performed to analyze genetic variation under the electron beam condition (2MeV/n and 0.5mA).

Variation Analysis of in vitro Cultured Plant Rhizomes Based on RAPD

Jaihyunk Ryu,Chang-Hyu Bae 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

RAPD (random amplified polymorphic DNA) analysis was examined to detect variation of in vitro cultured 30 rhizomes of Cymbidium goeringii and Cymbidium kanran, with long-term subculture, respectively. Out of 105 DNA bands detected, the 38 were polymorphic with a polymorphic rate 36.1% in the C. goeringii. Out of 126 DNA bands detected, the 58 were polymorphic with a polymorphic rate 46.0% in the C. kanran. The size of the amplified fragments of the two species ranged from about 200bp to 3,000bp. Genetic similarity matrix (GSM) shows from 0.810 to 1.00 with an average of 0.929 in the rhizomes of C. goeringii and 0.786 to 0.984 with an average of 0.915 in the C. kanran. The result indicate that the long-term in vitro cultured C. goeringii and C. kanran supplemented with growth regulators might be promoted in higher polymorphism and increased GSM. Thus, the higher polymorphism rate of in vitro cultured rhizomes might be resulted in long-term subculture and the plant growth regulators supplemented with the culture medium. The results provide as fundamental data to develop a new materials for plant breeding and resources plant.

Effect of Gamiojeoksan Remnants used as Fertilizer on Growth and Yield of Korean mint

Jaihyunk Ryu,Seongkyu Choi,Kyeong Won Yun,Youngnam Seo,Kyoungsun Seo,Hyunjin Kim 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.3

In these experiments Gamiojeoksan remnant components were analyzed to recycle this medicinal herb remnant fertilizers. The basic growth of Korean mint by application of Gamiojeoksan remnants were higher than control. As the amount of fertilizers were increased, plant height, stem diameter, number of leaves and number of branches were increased. The growth and yield were the highest in the treatment of 30 g/pot. Weight of whole plant of Korean mint has a tendency to be heavy in application of herbs remnant than that of control.

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (<i>Rubus fruticosus</i>)

Ryu, Jaihyunk,Ha, Bo-Keun,Kim, Dong Sub,Kim, Jin-Baek,Kim, Sang Hoon,Ahn, Joon-Woo,Jeong, Il Yun,Jo, Han-Jik,Kim, Ee-Yup,Kang, Si-Yong Cambridge University Press 2014 Plant genetic resources Vol.12 No.1

<P>Blackberry is a fruiting berry species with very high nutrient contents. With the recent increasing consumer demand for blackberries, new sources of germplasm and breeding techniques are required to improve blackberry production. This study was carried out to evaluate the genetic diversity (GD) and relationship among 55 blackberry (<I>Rubus fruticosus</I>) mutants derived from γ-ray treatment (52 lines) and <I>N</I>-methyl-<I>N</I>′-nitrosourea (MNU) treatment (three lines) using an inter-simple sequence repeat marker. A total of 18 bands were amplified with an average of 3.6 bands per primer. Among them, eight bands were identified to be polymorphic with a rate of 44.4%. In addition, the GD information content values were highest in the 60 Gy treatment population and the GD values were higher in the γ-ray treatment populations than in the MNU treatment population. According to a cluster analysis, all the mutant lines can be classified into five categories, and the genetic distance was greatest between the 80 Gy-irradiated population and other populations. These results indicate that mutant lines have high GD and can be effectively utilized for improving blackberry breeding.</P>

Morphological characteristics, chemical and genetic diversity of kenaf (Hibiscus cannabinus L.) genotypes

Ryu, Jaihyunk,Kwon, Soon-Jae,Kim, Dong-Gun,Lee, Min-Kyu,Kim, Jung Min,Jo, Yeong Deuk,Kim, Sang Hoon,Jeong, Sang Wook,Kang, Kyung-Yun,Kim, Se Won,Kim, Jin-Baek,Kang, Si-Yong The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.44 No.4

The kenaf plant is used widely as food and in traditional folk medicine. This study evaluated the morphological characteristics, functional compounds, and genetic diversity of 32 kenaf cultivars from a worldwide collection. We found significant differences in the functional compounds of leaves from all cultivars, including differences in levels of chlorogenic acid isomer (CAI), chlorogenic acid (CA), kaempferol glucosyl rhamnoside isomer (KGRI), kaempferol rhamnosyl xyloside (KRX), kaemperitrin (KAPT) and total phenols (TPC). The highest TPC, KAPT, CA, and KRX contents were observed in the C22 cultivars. A significant correlation was observed between flowering time and DM yield, seed yield, and four phenolic compounds (KGRI, KRX, CAI, and TPC) (P < 0.01). To assess genetic diversity, we used 80 simple sequence repeats (SSR) primer sets and identified 225 polymorphic loci in the kenaf cultivars. The polymorphism information content and genetic diversity values ranged from 0.11 to 0.79 and 12 to 0.83, with average values of 0.39 and 0.43, respectively. The cluster analysis of the SSR markers showed that the kenaf genotypes could be clearly divided into three clusters based on flowering time. Correlations analysis was conducted for the 80 SSR markers; morphological, chemical and growth traits were found for 15 marker traits (corolla, vein, petal, leaf, stem color, leaf shape, and KGRI content) with significant marker-trait correlations. These results could be used for the selection of kenaf cultivars with improved yield and functional compounds.

Assessment of Growth and Seed Oil Composition of Kenaf (Hibiscus cannabinus L.) Germplasm

Ryu, Jaihyunk,Ha, Bo-Keun,Kim, Dong Sub,Kim, Jin-Beak,Kim, Sang Hoon,Kang, Si-Yong 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4

This study was conducted to evaluate the growth characteristics and fatty acid composition among 15 kenaf mutants derived from the kenaf germplasm C14 and 15 kenaf accessions originating from Russia, India, China, Iran, and Italy. The overall growth performance (plant height, stem diameter, flowering date, leaf, and flower size) of the stem color mutant lines derived from C14 are similar to those of the original variety. However, the flower color mutant lines derived from C14 showed flowering to occur 10 days later when compared with the original variety and showed smaller leaf sizes than the original variety. Late-ripened kenaf accessions (Jinju, Auxu, and Jnagdae) can yield more bio-mass compared with early or medium-maturing germplasm. The late maturity kenaf (Auxu, Jinju, and Jangdae) has a higher oil percentage than the early maturity germplasm. Linoleic, oleic, and palmitic acids were the predominant fatty acids in all kenaf seeds. The stem color mutant lines significantly surpassed the parental means of all saturated fatty acids. In addition, the flower color mutant lines showed broad ranges of variation in oleic acid. The 15 accessions showed a wide range of fatty acid compositions, spanning from 29.75 to 38.30% saturated fatty acids and 61.70 to 70.24% total unsaturated fatty acids, and the late maturity kenaf has a higher linoleic acid percentage than the early maturity germplasm. The flowering period was highly positively ($P{\leq}0.01$) correlated with the plant height, stem diameter, oil percent, and linolenic acid ($C_{18:3}$), and it was significantly negatively ($P{\leq}0.01$) correlated with stearic acid ($C_{18:0}$). These results will provide valuable information to assist the parental selection of kenaf breeding.

Anthocyanin Contents Enhancement with Gamma Irradiated Mutagenesis in Blackberry (Rubus fructicosus)

Ryu, Hyung Won,Cho, Byoung Ok,Ryu, Jaihyunk,Jin, Chang Hyun,Kim, Jin-Baek,Kang, Si Yong,Han, Ah-Reum NATURAL PRODUCT COMMUNICATIONS 2017 Natural product communications Vol.12 No.9

<P>Effects of radiation processing on the endogenous phytochemical production in blackberry (Rubus fructicosus L.) were investigated through a metabolites analysis, using high-performance liquid chromatography with a diode array detector and a triple quadrupole mass spectrometer (HPLC-DAD-ESI-MS/MS). Four mutant cultivar lines (B, C, D, and E) were developed through the treatment of various doses of gamma irradiation (80, 60, 40, and 20 Gy) on the control blackberry cultivar (V3). These mutant cultivar lines were selected according to their different morphological variations in leaf shape and branch color. In the present study, we performed quantitative analyses of the four standard compounds in blackberry mutant cultivars: cyanidin-3-O-glucoside, cyanidin-3-Oxyloside, cyanidin-3-O-malonylglucoside, and cyanidin-3-O-dioxalylglucoside. The HPLC fingerprints displayed different profiles for six blackberry cultivar lines, demonstrating that the accumulation of total anthocyanins was affected by the gamma irradiation. The contents of cyanidin-3-O-glucoside and cyanidin-3-O-xyloside were high in the gamma-irradiated mutant cultivars of blackberries, compared to those of the control cultivar (V3) and the cross breeding cultivar (R. fructicosus x R. parvifolius; CB). Cyanidin-3-O-malonylglucoside and cyanidin-3-O-dioxalylglucoside were also analyzed for their quantification in six cultivar lines, as metabolites contributing to the discrimination of six cultivar lines. Among gamma irradiated mutant blackberry cultivars, the highest dose level of gamma irradiation (80 Gy) resulted in the highest cyanidin-3-O-glucoside content enhancement. Therefore, these results can be useful to determine the optimized gamma irradiated value at which anthocyanins reaches an abundant level in Rubus species.</P>