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가토에서 일측 요관 부분 및 완전폐색시 신장과 요관의 조직학적 변화
김상현,김용웅,육승모,한동석,노안식,임재성,김홍식,나용길,설종구 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2
This study was designed to clarify histologic changes in the experimental rabbit kidney and ureter after unilateral partial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, the partial obstruction followed by 4 weeks, the complete obstruction followed by 2 weeks, and the complete obstruction followed by 4 weeks. The normal control group was consisted of 5 adult white rabbits. The kidneys and ureters for light microscopy were fixed in 10% neutral formalin, embedded in paraffin and stained with hematoxylin and eosin. Abnormal histologic fingings showed in ipsilateral kidney and ureter of all subgroups(such as dilatation of uriniferous tubule, focal necrosis and desquamation of epithelium, interstitial fibrosis, glomerular congestion, widening of Bowman's space, dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis and interstitial inflammation). These findings were most severe in the group of the complete-obstructed ureter for 4 weeks. No significant changes showed in contralateral ureter of all subgroups. The degree of obstruction was severe and period of obstruction was long, the histological changes in ipsilateral side were severe. Despite of changes in ipsilateral side no abnormal findings showed in contralateral side. We suggest that long term experimental investigation of histologic changes in ipsilateral side and compensatory changes in contralateral side have to be studied.
Transgenic Efficiency of FoxN1-targeted Pig Parthenogenetic Embryos
Jae-Hoon Yeo,In-Sul Hwang,Jae Kyung Park,Dae-Jin Kwon,Seoki Im,Eung-Woo Park,Jeong-Woong Lee,Choon-Keun Park,Seongsoo Hwang 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.4
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. Therefore, we applied CRISPR/Cas9 system to generate FoxN1-targeted pig parthenogenetic embryos. Using single guided RNA targeted to pig FoxN1 genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. In results, regardless of the concentrations of vector, the cleavage rate were significantly (p<0.05) decreased (4 ng/μl, 51.24%; 8 ng/μl, 40.88%; and 16 ng/μl; 45.22%) compared to no injection group (70.44%). The blastocyst formation rates were also decreased in vector injected 3 groups (4 ng/μl, 7.96%; 8 ng/μl, 6.4%; and 16 ng/μl; 9.04%) compared to no injection group (29.07%). In addition, the blastocyst formation rates between sham injected group (13.51%) and no injection group (29.07%) also showed significant difference (p<0.05). The mutation rates were comparable between groups (4 ng/μl, 18.4%; 8 ng/μl, 12.5%; and 16 ng/μl; 20.0%). The sequencing analysis showed that blastocysts derived from each group were successfully mutated in FoxN1 loci regardless of the vector concentrations. However, the deletion patterns were higher than the patterns of point mutation and insertion regardless of the vector concentrations. In conclusion, we described that cytoplasmic microinjection of FoxN1-targeted CRISPR/Cas9 vector could efficiently generate transgenic pig parthenogenetic embryos in one-step.
Yang, Jae Wook,Ham, Dong Sik,Kim, Hyun Woong,Lee, Sul Gee,Park, Sae Kwang,Seo, Su Kil Springer-Verlag 2012 Graefe’s archive for clinical and experimental oph Vol.250 No.1
<P>Ocular immune privilege is a multifactorial phenomenon evolutionally selected to prevent immunogenic inflammation from disrupting the visual axis and causing blindness. Here, we investigated the role of signal transducers and activators of transcription (Stat3) and indoleamine 2,3-dioxygenase (IDO) in ocular immune privilege in corneal stromal cells.</P>
Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer
In-Sul Hwang,Dae-Jin Kwon,Keun Bong Oh,Sun-A Ock,Hak-Jae Chung,In-Cheol Cho,Jeong-Woong Lee,Gi-Sun Im,Seongsoo Hwang 한국발생생물학회 2015 발생과 생식 Vol.19 No.2
The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 μM roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were 98 ± 35.2 and 145 ± 11.2, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.
Transgenic Efficiency of FoxN1-targeted Pig Parthenogenetic Embryos
Yeo, Jae-Hoon,Hwang, In-Sul,Park, Jae Kyung,Kwon, Dae-Jin,Im, Seoki,Park, Eung-Woo,Lee, Jeong-Woong,Park, Choon-Keun,Hwang, Seongsoo The Korean Society of Embryo Transfer 2014 한국동물생명공학회지 Vol.29 No.4
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. Therefore, we applied CRISPR/Cas9 system to generate FoxN1-targeted pig parthenogenetic embryos. Using single guided RNA targeted to pig FoxN1 genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. In results, regardless of the concentrations of vector, the cleavage rate were significantly (p<0.05) decreased ($4ng/{\mu}l$, 51.24%; $8ng/{\mu}l$, 40.88%; and $16ng/{\mu}l$; 45.22%) compared to no injection group (70.44%). The blastocyst formation rates were also decreased in vector injected 3 groups ($4ng/{\mu}l$, 7.96%; $8ng/{\mu}l$, 6.4%; and $16ng/{\mu}l$; 9.04%) compared to no injection group (29.07%). In addition, the blastocyst formation rates between sham injected group (13.51%) and no injection group (29.07%) also showed significant difference (p<0.05). The mutation rates were comparable between groups ($4ng/{\mu}l$, 18.4%; $8ng/{\mu}l$, 12.5%; and $16ng/{\mu}l$; 20.0%). The sequencing analysis showed that blastocysts derived from each group were successfully mutated in FoxN1 loci regardless of the vector concentrations. However, the deletion patterns were higher than the patterns of point mutation and insertion regardless of the vector concentrations. In conclusion, we described that cytoplasmic microinjection of FoxN1-targeted CRISPR/Cas9 vector could efficiently generate transgenic pig parthenogenetic embryos in one-step.
Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer
Hwang, In-Sul,Kwon, Dae-Jin,Oh, Keun Bong,Ock, Sun-A,Chung, Hak-Jae,Cho, In-Cheol,Lee, Jeong-Woong,Im, Gi-Sun,Hwang, Seongsoo The Korean Society of Developmental Biology 2015 발생과 생식 Vol.19 No.2
The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 ${\mu}M$ roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were $98{\pm}35.2$ and $145{\pm}11.2$, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.
포노 사피엔스 시대 스마트시티의 다목적 공간 공유를 위한 서비스 디자인 연구
이웅희(Lee Woong Hee),김정우(Kim Jung Woo),설상훈(Sul Sang Hun),최재붕(Choi Jae Boong) 한국IT서비스학회 2019 한국IT서비스학회 학술대회 논문집 Vol.2019 No.2
새로운 인류인 포노 사피엔스는 스마트폰을 활용하여 언제 어디서나 소통하고 정보를 공유하면서 새로운 도시 개념을 확장시켰다. 이는 기술 혁신 도시인 스마트시티로써 도출 된 데이터를 활용하여 시민들의 삶의 질을 향상시키고 있다. 스마트시티 시민들은 물리공간에서 다목적 공간 공유를 위해 용도에 따라 서비스를 제공하지만 각 구역 간에 공간과 공간의 거리가 멀어지면서 이동시 어려움을 발생한다. 본 논문에서는 포노 사피엔스 시대스마트시티의 다목적 공간과 공간 사이의 거리를 단축시키는 서비스 디자인을 제안한다.