DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Life history responses of the cabbage beetle Colaphellus bowringi to temperature change

Jian‐Jun Tang,Hai-Min HE,Ting GENG,Shu FU,Fang-Sen XUE 한국곤충학회 2016 Entomological Research Vol.46 No.6

Temperature is considered one of the most important mediators of phenotypic plasticity in ectotherms. Here, we investigated life history traits of the cabbage beetle, Colaphellus bowringi Baly (Coleoptera: Chrysomelidae), at a wide range of temperatures (16, 19, 22, 24, 26 and 28°C). The larval and pupal times were significantly decreased with increasing rearing temperature and growth rate was positively correlated with temperature. However, the relationship between body size and rearing temperature in C. bowringi did not follow the temperature–size rule; both males and females reached the highest body weight at 19°C. Females were significantly larger than males at all temperatures. Male pupae lost significantly more weight at metamorphosis compared to females. However, diapausing males gained significantly higher weight after feeding compared to diapausing females at higher temperatures of 22, 24, 26 and 28°C. Body weight tended to decrease with increasing rearing temperature, whereas sexual size dimorphism (SSD) tended to increase with increasing rearing temperature; thus, Rensch's rule is upheld. The degree to which SSD changed with temperature varied with different development stages. SSD was lowest in pupae, highest in newly emerged adults and intermediate in diapausing adults.

Department of Bioengineering

Jian?He Xu 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

Roles of Immunohistochemical Staining in Diagnosing Pulmonary Squamous Cell Carcinoma

Yan, Yue,Zhang, Ya-Xiong,Fang, Wen-Feng,Kang, Shi-Yang,Zhan, Jian-Hua,Chen, Nan,Hong, Shao-Dong,Liang, Wen-Hua,Tang, Yan-Na,He, Da-Cheng,Wu, Xuan,Zhang, Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.

A Continuous Abnormal Speech Detection Method Based on Time Domain features Weighted

He Jun,Ji-chen Yang,Qing-hua Zhang,Guo-xi Sun,Jian-bing Xiong 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12

In this brief, a novel pathological continuous speech detection method based on time domain features weighted. First, different optimal threshold for time domain features, including zero crossing ratio, short-time energy and autocorrelation, are obtained from training speech data. Second, a difference evaluation technique is proposed, and with it, the difference of the same time domain feature selected from testing speech data and training speech data were obtained. Finally, to distinguish a given speech well, a novel weighting method based on difference evaluation for each kinds of time domain is employed, respectively. Experiments were conducted on the pathological speech database to prove the power and effectiveness of the proposed method. Results obtained shown that this method outperforms other early proposed time domain feature method, creating a more reliable technique for pathological continuous speech detection.

Effects of the Maceration Enzymes on Evolution of Pyranoanthocyanins and Cinnamic Acids During the Cabernet Gernischet (Vitis vinifera L. cv.) Red Wine Making

Jian-Jun He,Fu-Liang Han,Qing-Quan Yu,Qiu-Hong Pan,Chang-Qing Duan,Guo-Li Cheng 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3

The effects of the maceration enzymes on the evolution of 4-vinylphenols pyranoanthocyanins and cinnamic acids were investigated during the Cabernet Gernischet red wine making. The results showed that the contents of p-coumaric acid and caffeic acid in the maceration enzyme-treated wine (the treated wine) were higher than those in the maceration enzyme-untreated wine (the control wine) at the alcoholic fermentation (AF),maceration (M), and malolactic fermention (MLF) stages. Malvidin-3-O-glucoside-4-vinylphenol (Mv-glu-vp) and malvidin-3-O-(6-O-acetyl)-glucoside-4-vinylphenol (Mvacet-glu-vp) were formed at the AF and M stages in the treated wine, while they were formed at the end of MLF stage in the control wine. However, the contents of these 2kinds of 4-vinylphenol pyranoanthocyanins in the control wine were higher than those in the treated wine during aging. These results showed that the use of maceration enzymes in winemaking can promote p-coumaric acid and p-caffeic acid transformed to vinylphenols and accelerate the formation of pyranoanthocyanins.

Research on the Positioning of Work Machines in Greenhouse Using Bluetooth Low Energy Indoor Detection Technology

( Jian-xin He ),( Cang-qi Li ),( Ming-yen Lin ),( Chyung Ay ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Outdoor is using a mobile device combined with global positioning system (GPS) for positioning, the most popular application today; and indoor is based on the signal strength of Bluetooth, Wi-Fi, ZigBee and other wireless communication technology as a positioning basis. This research uses a fruit harvester for positioning in a greenhouse. However, the positioning accuracy of GPS in a greenhouse is easily affected and unable to be precise due to obstacles, communication channels, and multiple paths. To solve this problem, this study used iBeacon Bluetooth 4.0 module as a communication device for indoor positioning. In the experiment, ten iBeacons were used as the sending end, placed at various points in the room to serve as a positioning basis, and then installed an iBeacon combined with the Arduino on a fruit harvester as the receiving end. Then use the intensity of the sending and receiving signal as the basis, with the least square method, to calculate the corresponding distance between the signal receiving point and the sending point, and finally estimate the position of the fruit harvester by triangulation. At present, the preliminary experiment results show that, with the classroom as the test space, the indoor positioning success rate can reach 77% within 3 meters of the error value. The same method will be applied to find out the best positioning method for the harvester in the greenhouse.

Calculation of Kappa-Averaged Collision Strengths of Silicon VIII Ion for a Non-Maxwellian Distribution

Jian He,Qingguo Zhang 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.9

Non-Maxwellian distributions are found in the laboratory and space plasmas. For an accurate study of plasmas, the Kappa-averaged collision strengths of silicon VIII ion for 40S3=2-20D3=2, 40S3=2-20D5=2 and 20D3=2-20D5=2 transitions are calculated for Kappa distributions with = 2, 3 and 5 and for temperatures from 104:5 K to 106:5 K. Results indicate that significant differences occur between the averaged collision strengths for the Maxwellian and the Kappa distributions. Fuythermore, and for each value, the Kappa-averaged collision strengths vary in a complicated way with temperature for the 40S3=2-20D3=2 and 40S3=2-20D5=2 transitions while they decrease with increasing temperature for the 20D3=2-20D5=2 transition. The calculation is significant if plasmas are to be studied for a non-Maxwellian distribution.

Complement Receptor 1 Expression in Peripheral Blood Mononuclear Cells and the Association with Clinicopathological Features And Prognosis of Nasopharyngeal Carcinoma

He, Jian-Rong,Xi, Jing,Ren, Ze-Fang,Qin, Han,Zhang, Ying,Zeng, Yi-Xin,Mo, Hao-Yuan,Jia, Wei-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Purpose: Complement receptor 1 (CR1) is induced by Epstein-Barr virus (EBV) and may be a potential biomarker of nasopharyngeal carcinoma (NPC). We conducted the present study to evaluate the association of CR1 expression with clinicopathological features and prognosis of NPC. Methods: We enrolled 145 NPC patients and 110 controls. Expression levels of CR1 in peripheral blood mononuclear cells (PBMCs) were detected using quantitative real-time PCR and associations with clinicopathological features and prognosis were examined. Results: CR1 levels in the NPC group [3.54 (3.34, 3.79)] were slightly higher than those in the controls [3.33 (3.20, 3.47)] (P<0.001). Increased CR1 expression was associated with histology classification (type III vs. type II, P=0.002), advanced clinical stage (P=0.003), high T stage (P=0.017), and poor overall survival (HR, 4.89; 95% CI, 1.23-19.42; P=0.024). However, there were no statistically significant differences in CR1 expression among N or M stages. Conclusion: These findings indicate that CR1 expression in PBMCs may be a new biomarker for prognosis of NPC and a potential therapeutic target.

PROXIMAL POINTS METHODS FOR GENERALIZED IMPLICIT VARIATIONAL-LIKE INCLUSIONS IN BANACH SPACES

He, Xin-Feng,Lou, Jian,He, Zhen The Youngnam Mathematical Society 2012 East Asian mathematical journal Vol.28 No.1

In this paper, we study generalized implicit variational-like inclusions and $J^{\eta}$-proximal operator equations in Banach spaces. It is established that generalized implicit variational-like inclusions in real Banach spaces are equivalent to fixed point problems. We also establish relationship between generalized implicit variational-like inclusions and $J^{\eta}$-proximal operator equations. This equivalence is used to suggest a iterative algorithm for solving $J^{\eta}$-proximal operator equations.