Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

조기이유자돈 사료내 볶은 대두박의 수준이 자돈의 성장에 미치는 영향

문홍길,김지훈,조원탁,신인수,한인규 ( H . K . Moon,J . H . Kim,W . T . Cho,I . S . Shin,In K . Han ) 한국영양사료학회 1998 韓國營養飼料學會誌 Vol.22 No.3

Proposed mechanism in the change of cellular composition in the outer medullary collecting duct during potassium homeostasis.

Park, E-Y,Kim, W-Y,Kim, Y-M,Lee, J-H,Han, K-H,Weiner, I D,Kim, J Gutenberg 2012 HISTOLOGY AND HISTOPATHOLOGY Vol.27 No.12

<P>Potassium depletion (K?-D) induces hypertrophy and hyperplasia of collecting duct cells, and potassium repletion (K?-R) induces regression of these changes. The purpose of this study was to examine the time courses of the changes in cellular composition, the origin of intercalated cells (ICs) and the mechanism responsible for these changes. SD rats received K?-depleted diets for 1, 7, or 14 days. After K?-D for 14 days some of the rats received normal diets for 1, 3, 5, or 7 days. In the inner stripe of the outer medulla, K?-D increased significantly the number and proportion of H?-ATPase-positive ICs, but decreased the proportion of H?-ATPase-negative principal cells (PCs). However, proliferation was limited to H?-ATPase-negative PCs. During K?-R, the cellular composition was recovered to control level. Apoptosis increased during K?-R and exclusively limited in H?-ATPase-negative PCs. Double immunolabeling with antibodies to PC and IC markers identified both cells negative or positive for all markers during both K?-D and K?-R. Electron microscopic observation showed that ultrastructure of AE1-positive some cells were similar to AE1-negative some cells during K?-R. LC3 protein expression increased significantly and autophagic vacuoles appeared particularly in PCs on days 14 of K?-D and in ICs on days 3 of K?-R. These results suggest that PCs and ICs may interconvert in response to changes in dietary K+ availability and that autophagic pathways may be involved in the interconversion.</P>

사료내 전해질 공급제의 종류가 이유자돈의 성장 , 뇨성상 및 영양소 이용율에 미치는 영향

박재환(J . H . Park),한인규(I . K . Han) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.3

KMT-2016-BLG-0212: First KMTNet-Only Discovery of a Substellar Companion

K.-H. Hwang,H.-W. Kim,D.-J. Kim,A. Gould,M. D. Albrow,S.-J. Chung,C. Han,Y. K. Jung,Y.-H. Ryu,I.-G. Shin,Y. Shvartzvald,J. C. Yee,W. Zang,W. Zhu,S.-M. Cha,S.-L. Kim,C.-U. Lee,D.-J. Lee,Y. Lee,B.-G. Pa 한국천문학회 2018 Journal of The Korean Astronomical Society Vol.51 No.6

We present the analysis of KMT-2016-BLG-0212, a low flux-variation $(I_{\rm flux-var}\sim 20$ mag) microlensing event, which is in a high-cadence ($\Gamma = 4 {\rm hr}^{-1}$) field of the three-telescope Korea Microlensing Telescope Network (KMTNet) survey. The event shows a short anomaly that is incompletely covered due to the brief visibility intervals that characterize the early microlensing season when the anomaly occurred. We show that the data are consistent with two classes of solutions, characterized respectively by low-mass brown-dwarf $(q=0.037)$ and sub-Neptune $(q<10^{-4})$ companions. Future high-resolution imaging should easily distinguish between these solutions.

Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer

Han, S-W,Oh, D-Y,Im, S-A,Park, S R,Lee, K-W,Song, H S,Lee, N-S,Lee, K H,Choi, I S,Lee, M H,Kim, M A,Kim, W H,Bang, Y-J,Kim, T-Y Nature Publishing Group 2009 The British journal of cancer Vol.100 No.2

<P>This prospective study was conducted with the Korean Cancer Study Group to evaluate the efficacy and safety of cetuximab combined with modified FOLFOX6 (mFOLFOX6) as first-line treatment in recurrent or metastatic gastric cancer and to identify potential predictive biomarkers. Patients received cetuximab 400 mg m<SUP>−2</SUP> at week 1 and 250 mg m<SUP>−2</SUP> weekly thereafter until disease progression. Oxaliplatin (100 mg m<SUP>−2</SUP>) and leucovorin (100 mg m<SUP>−2</SUP>) were administered as a 2-h infusion followed by a 46-h continuous infusion of 5-fluorouracil (2400 mg m<SUP>−2</SUP>) every 2 weeks for a maximum of 12 cycles. Biomarkers potentially associated with efficacy were analysed. Among 38 evaluable patients, confirmed response rate (RR) was 50.0% (95% CI 34.1–65.9). Median time-to-progression (TTP) was 5.5 months (95% CI 4.5–6.5) and overall survival (OS) 9.9 months. Eleven patients having tumour EGFR expression by immunohistochemistry with low serum EGF and TGF-<I>α</I> levels showed a 100% RR compared to 37.0% in the remaining 27 patients (<I>P</I><0.001). Moreover, ligand level increased when disease progressed in seven out of eight patients with EGFR expression and low baseline ligand level. No patient exhibited EGFR amplification or K-ras mutations. Gastric cancer patients with EGFR expression and low ligand levels had better outcomes with cetuximab/mFOLFOX6 treatment.</P>

USING ORBITAL EFFECTS TO BREAK THE CLOSE/WIDE DEGENERACY IN BINARY-LENS MICROLENSING EVENTS

Shin, I.-G.,Sumi, T.,Udalski, A.,Choi, J. Y.,Han, C.,Gould, A.,Abe, F.,Bennett, D. P.,Bond, I. A.,Botzler, C. S.,Chote, P.,Freeman, M.,Fukui, A.,Furusawa, K.,Harris, P.,Itow, Y.,Ling, C. H.,Masuda, K. IOP Publishing 2013 The Astrophysical journal Vol.764 No.1

<P>Microlensing can provide an important tool to study binaries, especially those composed of faint or dark objects. However, accurate analysis of binary-lens light curves is often hampered by the well-known degeneracy between close (s < 1) and wide (s > 1) binaries, which can be very severe due to an intrinsic symmetry in the lens equation. Here, s is the normalized projected binary separation. In this paper, we propose a method that can resolve the close/wide degeneracy using the effect of a lens orbital motion on lensing light curves. The method is based on the fact that the orbital effect tends to be important for close binaries while it is negligible for wide binaries. We demonstrate the usefulness of the method by applying it to an actually observed binary-lens event MOA-2011-BLG-040/OGLE-2011-BLG-0001, which suffers from severe close/wide degeneracy. From this, we are able to uniquely specify that the lens is composed of K- and M-type dwarfs located similar to 3.5 kpc from the Earth.</P>

Protective effect of EC-18, a synthetic monoacetyldiglyceride on lung inflammation in a murine model induced by cigarette smoke and lipopolysaccharide

Shin, I.S.,Ahn, K.S.,Shin, N.R.,Lee, H.J.,Ryu, H.W.,Kim, J.W.,Sohn, K.Y.,Kim, H.J.,Han, Y.H.,Oh, S.R. Elsevier Science 2016 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.30 No.-

The antler of Sika deer (Cervus nippon Temminck) has been used a natural medicine in Korea, China and Japan, and a monoacetyldiaglyceride (1-palmitoyl-2-linoleoyl-3-acetylglycerol, PLAG) was found in the antler of Sika deer as a constituent for immunomodulation. In this study, we investigated protective effects of EC-18 (a synthetic copy of PLAG) on inflammatory responses using a cigarette smoke with lipopolysaccharide (LPS)-induced airway inflammation model. Mice were exposed to cigarette smoke for 1h per day for 3days. Ten micrograms of LPS dissolved in 50μL of PBS was administered intra nasally 1h after the final cigarette smoke exposure. EC-18 was administered by oral gavage at doses of 30 and 60mg/kg for 3days. EC-18 significantly reduced the number of neutrophils, reactive oxygen species production, cytokines and elastase activity in bronchoalveolar lavage fluid (BALF) compared with the cigarette smoke and LPS induced mice. Histologically, EC-18 attenuated airway inflammation with a reduction in myeloperoxidase expression in lung tissue. Additionally, EC-18 inhibited the phosphorylation of NF-κB and IκB induced by cigarette smoke and LPS exposure. Our results show that EC-18 effectively suppresses neutrophilic inflammation induced by cigarette smoke and LPS exposure. In conclusion, this study suggests that EC-18 has therapeutic potential for the treatment of chronic obstructive pulmonary disease.

환원유를 혼입한 생유의 검출방법에 관한 연구

한인규 ( I K Han ),김현욱 ( H U Kim ),육종융 ( C Y Yuk ),허환녕 ( H Y Huh ) 한국축산학회 1968 한국축산학회지 Vol.10 No.1

This experiment was conducted to develope a method of detection for adulteration of raw milk with spray-dried milk powder. Five ㎖. of milk to be tested was treated with 4 ㎖. of 15% ammonium moybdate solution (w/v). After being shaken, the tube was heated at 77.50℃ for 90 minutes. In order to estimate the intensity of color, the methods involved diluting the 3 ㎖. of the sample treated above to 15 ㎖. with distilled H₂O, precipitating with 10% TCA solution and filtering. The degree of blue color developed upon heating was measured by the coleman spectrophotometer at 490 mμ and hemoglobinometer. An attempt was made to explain the mechanism for which the blue color was developed. The results obtained are summarized as follows; I. Raw milk resulted a light color, whereas the milk which has been reconstituted with spray-dried milk powder gave a deep blue color. There was a highly positive correlation (r=0.94) between the amount of spray-dried milk added in raw milk and intensity of blue color developed. Results revealed that this method could be used to detect 4% of addition of reconstituted milk with spray-dried milk powder. 3. It was also found that there was a positive correlation (r=0.97) between adulteration and Hb-meter reading. Eight percentage or more of adding reconstituted milk could bed erected by Hb-meter reading. 3. It was also found a straight linear relationship (r=0.95) between intensity of blue color and reducing capacity of milk. The reducing capacity of spray-dried milk was much higher than that of raw milk. 4. The cause of developing blue color described in this method could be resulted from the occurrence of inorganic phosphate and reducing capacity of the milk and the difference in blue color was due to the difference in reducing capacity between raw and reconstituted milk.

Therapeutic effect of Mahaenggamseok-tang on neutrophilic lung inflammation is associated with NF-κB suppression and Nrf2 activation

Kim, K.H.,Lee, J.Y.,Kwun, M.J.,Choi, J.Y.,Han, C.W.,Ha, K.T.,Jeong, S.I.,Jeong, H.S.,Joo, M. Elsevier Sequoia 2016 Journal of Ethnopharmacology Vol.192 No.-

Ethnopharmacological relevance: Mahaenggamseok-tang (MHGST), an herbal formula in traditional Asian medicine, has been used to treat patients with various pulmonary diseases including common cold and influenza. However, the potential therapeutic effect of MHGST on acute lung injury (ALI), a leading cause of death worldwide, and the anti-inflammatory mechanisms of MHGST remained less understood. Materials and methods: The methanol extract of MHGST was prepared and fingerprinted by HPLC. For the induction of ALI, C57BL/6 mice (n=5/group) received a single intraperitoneal (i.p.) injection of LPS. Referring to the dose for patients, two different amounts of MHGST were delivered in an aerosol to mouse lungs via trachea 2h after the i.p. LPS administration. Lung histology, bronchoalveolar lavage fluid, myeloperoxidase (MPO) activity, and the expression of inflammatory and Nrf2-dependent genes were analyzed to determine the effect of MHGST on lung inflammation. For mechanistic studies, western blotting and semi-quantitative RT-PCR were conducted using RAW 264.7 cells. Results: When administered 2h after the onset of ALI, MHGST relieved lung pathology characteristic to ALI, with decreases of neutrophil infiltration and MPO activity. While suppressing the expression of inflammatory genes, MHGST increased the expression of Nrf2-dependent genes in ALI mouse lungs. Concordantly, MHGST activated Nrf2 activity while suppressing NF-κB in RAW 264.7 cells. Conclusion: MHGST suppressed neutrophilic lung inflammation, a hallmark of ALI, which was associated with the activation of anti-inflammatory Nrf2 and the suppression of pro-inflammatory NF-κB. Our results suggest that MHGST has a therapeutic potential against ALI.