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정민용,임형택,백현동 경남대학교 환경문제연구소 2000 환경연구 Vol.23 No.-
In order to select probiotics with a high survival rate in gut and the growth inhibition of virulent pathogens to human beings or animals, we have examined a variety of microorganisms to assess the artificial gastric acid and bile acid. In addition, microbiological identification, antimicrobial activity, enzyme activity, and antibiotic resistance were performed. The selected strains survived for 24 h in artificial bile after incubation for 2 h in artificial gastric juice. Especially, MY4 strain showed higher survival for 2 h incubation in artificial gastric juice. As a result of API CHL 50 kit test, strains MY4, PT77, and PS-490 were tentatively identified as Lactobacillus plantarum, while strain PS653 was tentatively identified as Lactococcus lactis. All lactic acid bacteria were shown to inhibit the growth of Listeria monocytogenes ATCC 19111 dramatically within 24 h of incubation. In test of API ZYM kit, β-glucuronidase was not produced by any kind of lactic acid bacteria. All strains were resistant to antibiotics such as nisin, tetracycline, streptomycin, and rifamycin.
Hyung Seok Yu,Na Kyoung Lee,Hye Lin Jeon,Su Jin Eom,Mi Young Yoo,Sang Dong Lim,Hyun Dong Paik 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.3
Benzoic acid is occasionally used as a raw material supplement in food products and is sometimes generated during the fermentation process. In this study, the production of naturally occurring yogurt preservatives was investigated for various starter cultures and incubation temperatures, and considered food regulations. Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus plantarum, Bifidobacterium longum, Bifidobacterium lactis, Bifidobacterium bifidum, Bifidobacterium infantis, and Bifidobacterium breve were used as yogurt starter cultures in commercial starters. Among these strains, L. rhamnosus and L. paracasei showed the highest production of benzoic acid. Therefore, the use of L. rhamnosus, L. paracasei, S. thermophilus, and different incubation temperatures were examined to optimize benzoic acid production. Response surface methodology (RSM) based on a central composite design was performed for various incubation temperatures (35-44°C) and starter culture inoculum ratios (0-0.04%) in a commercial range of dairy fermentation processes. The optimum conditions were 0.04% L. rhamnosus, 0.01% L. paracasei, 0.02% S. thermophilus, and 38.12°C, and the predicted and estimated concentrations of benzoic acid were 13.31 and 13.94 mg/kg, respectively. These conditions maximized naturally occurring benzoic acid production during the yogurt fermentation process, and the observed production levels satisfied regulatory guidelines for benzoic acid in dairy products.
( Hyung-seok Yu ),( Na-kyoung Lee ),( Won-ju Kim ),( Do-un Lee ),( Jong-ha Kim ),( Hyun-dong Paik ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.5
The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach. Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K<sub>2</sub>HPO<sub>4</sub>, potassium citrate, Lcysteine phosphate, MgSO<sub>4</sub>, and MnSO<sub>4</sub>) were screened by using the Plackett-Burman design. Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM). The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K<sub>2</sub>HPO<sub>4</sub>, 5.0 g/l sodium acetate, 0.1 g/l MgSO<sub>4</sub>·7H<sub>2</sub>O, 0.05 g/l MnSO<sub>4</sub>·H<sub>2</sub>O, and 1.0 g/l Tween 80. The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS). After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l). Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM. To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37℃ with or without a pH control at 6.0 ± 0.1. The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium. In conclusion, OM may be utilized for mass producing JW15 for industrial use.
Nonalcoholic Fatty Liver Disease and Progression of Coronary Artery Calcification: A Cohort Study
( Dong Hyun Sinn ),( Danbee Kang ),( Yoosoo Chang ),( Seungho Ryu ),( Seonhye Gu ),( Hyunkyoung Kim ),( Donghyeong Seong ),( Soo Jin Cho ),( Byoung-kee Yi ),( Hyung-doo Park ),( Seung Woon Paik ),( Yo 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Nonalcoholic fatty liver disease (NAFLD), a hepatic manifestationof the metabolic syndrome, was associated with subclinical atherosclerosis atherosclerosisin many crosssectional studies, but the prospective associationbetween NAFLD and the progression of atherosclerosis has not beenevaluated. This study was conducted to evaluate the association betweenNAFLD and the progression of coronary atherosclerosis.Methods: This cohort study included 4,731 adult men and womenwith no history of CVD, liver disease or cancer at baseline who participatedin a repeated regular health screening exam between 2004and 2013. Fatty liver was diagnosed by ultrasound based on standardcriteria, including parenchymal brightness, liver-to-kidney contrast,deep beam attenuation and bright vessel walls. Progression of coronaryartery calcium (CAC) scores was measured using multidetectorCT scanners.Results: The annual rate of CAC progression in participants with andwithout NAFLD were 22% (95% confidence interval 20 - 23%) and17% (16 - 18%), respectively (p<0.001). The multivariable ratio ofprogression rates comparing participants with NAFLD to those withoutNAFLD was 1.04 (1.02 - 1.05; p<0.001). The association betweenNAFLD and CAC progression was similar in most subgroups analyzed,including in participants with CAC 0 and in those with CAC > 0at baseline.Conclusions: In this large cohort study of adult men and womenwith no history of CVD, NAFLD was significantly associated with thedevelopment of CAC independently of cardiovascular and metabolicrisk factors. NAFLD may play a pathophysiologic role in atherosclerosisdevelopment and may be useful to identify subjects with a higherrisk of subclinical disease progression.
Current trends in the management of pancreatic cystic neoplasms in Korea: a national survey
( Hyung Ku Chon ),( Sung Hoon Moon ),( Sang Wook Park ),( Woo Hyun Paik ),( Chang Nyol Paik ),( Byoung Kwan Son ),( Tae Jun Song ),( Dong Won Ahn ),( Eaum Seok Lee ),( Yun Nah Lee ),( Yoon Suk Lee ),( 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1
Background/Aims: The study aimed to investigate the current practice patterns in the management of pancreatic cystic neoplasms in Korea. Methods: An electronic survey was systematically distributed by email to members of the Korean Pancreatobiliary Association from December 2019 to February 2020. Results: In total, 115 (110 gastroenterologists, five surgeons) completed the survey, 72.2% of whom worked in a tertiary/ academic medical center. Most (65.2%) followed the 2012/2017 International Association of Pancreatology guidelines for the management of pancreatic cystic neoplasms. A gadolinium-enhanced magnetic resonance imaging/magnetic resonance cholangiopancreatography was the most common first-line diagnostic modality (42.1%), but a contrast-enhanced computed tomography scan was preferred as a subsequent surveillance tool (58.3%). Seventy-four percent of respondents routinely performed endoscopic ultrasound-guided fine needle aspiration for pancreatic cystic neoplasms with suspicious mural nodules. Endoscopic ultrasound-guided fine needle aspiration cytology (94.8%) and cystic fluid carcinoembryonic antigen (95.7%) were used for cystic fluid analysis. Most (94%) typically recommended surgery in patients with high-risk stigmata, but 18.3% also considered proceeding with surgery in patients with worrisome features. Most (96.5%) would continue surveillance of pancreatic cystic neoplasms for more than 5 years. Conclusions: According to this survey, there was variability in the management of pancreatic cystic neoplasms among the respondents. These results suggest that the development of evidence-based guidelines for pancreatic cystic neoplasms that fit the Korean practice is needed to create an optimal approach to the management of pancreatic cystic neoplasms.
Shelf Life Extension of Korean Fresh Pasta by Modified Atmosphere Packaging
Dong Sun Lee,Hyun-Dong Paik,Geun-Hyung Im,Ik-Hyun Yeo 한국식품영양과학회 2001 Preventive Nutrition and Food Science Vol.6 No.4
Fresh pasta was packaged in a modified atmosphere of 22% CO₂ /78% N₂, compared with a control air package for its quality changes during storage at 8℃. The modified atmosphere packaging suppressed the microbial growth of total aerobic bacteria, yeasts/molds with a concomitant reduction in the rates of physical, chemical quality changes,, thus successfully extended the shelf life of fresh pasta from 20 days of air packs to 40 days based on microbial criterion of 10^6 cfu/g. The shelf life extension was greater when the initial microbial quality of the product was better
( Hyung-seok Yu ),( Na-kyoung Lee ),( Ae-jin Choi ),( Jeong-sook Choe ),( Chun Ho Bae ),( Hyun-dong Paik ) 한국미생물·생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.7
Probiotics are known to provide the host with immune-modulatory effects and are therefore of remarkable interest for therapeutic and prophylactic applications against various disorders, including inflammatory diseases. Weissella cibaria JW15 (JW15) has been reported to possess probiotic and antioxidant properties. However, the effect of JW15 on inflammatory responses has not yet been reported. Therefore, the objective of the current study was to evaluate the anti-inflammatory potential of JW15 against lipopolysaccharide (LPS) stimulation. The production of pro-inflammatory factors and the cellular signaling pathways following treatment with heat-killed JW15 was examined in LPS-induced RAW 264.7 cells. Treatment with heat-killed JW15 decreased nitric oxide and prostaglandin E2 production via downregulation of the inducible nitric oxide synthase and cyclooxygenase-2. In addition, treatment with heat-killed JW15 suppressed the expression of pro-inflammatory cytokines, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. The anti-inflammatory properties of treating with heat-killed JW15 were associated with mitogen-activated protein kinase signaling pathwaymediated suppression of nuclear factor-κB. These results indicated that JW15 possesses antiinflammatory potential and provide a molecular basis regarding the development of functional probiotic products.
Shelf Life Extension of Korean Fresh Pasta by Modified Atmosphere Packaging
Lee, Dong-Sun,Paik, Hyun-Dong,Im, Geun-Hyung,Yeo, Ik-Hyun The Korean Society of Food Science and Nutrition 2001 Preventive Nutrition and Food Science Vol.6 No.4
Fresh pasta was packaged in a modified atmosphere of 22% $CO_2$/78% $N_2$ and compared with a control air package for its quality changes during storage at 8$^{\circ}C$. The modified atmosphere packaging suppressed the microbial growth of total aerobic bacteria and yeasts/molds with a concomitant reduction in the rates of physical and chemical quality changes, and thus successfully extended the shelf life of fresh packs from 20 days of air packs to 40 days based on microbial criterion of 10$^{6}$ cfu/g. The shelf life extension was greater when the initial microbial quality of the product was better.