손마사지가 허혈성 심질환 환자의 불안에 미치는 효과

현경선,이향련,공송심,윤경자,김현섭,김효남,최지원,김운정 성인간호학회 2001 성인간호학회지 Vol.13 No.2

The purpose of this study was to investigate the effect of a hand massage program on anxiety, vital sign and pain in clients with ischemic heart disease. The design utilized for this stuffy was quail-experimental with a nonequivalent control group non-synchronized design The subjects were fifty-four patients, twenty-eight for the experimental and twenty-six for the control group, who were admitted with ischemic heart diease at a cardiac intensive care unfit in K medical center of K university. This study was carried out from May, 1999 to March, 2000. The level of anxiety and pain measured by Visual Analogue Scale, systolic blood pressure, diastolic blood pressure and pulse rate were measured before and after hand massage, the state of Anxiety was measured by the Spielberger (1970) scale at admission and after hand massage for three days. The collected data were processed by using the SPSS PC program and analyzed using χ2-test and t-test. The result of this study are as follows : 1. The scores of VAS anxiety and State of anxiety of the experimental group were lower than those of the control group. 2. The degree of systolic blond pressure, diastolic blood pressure and pulse rate of the experimental group were lower than that of the control group. 3. The score of VAS pain of the experimental group was lower than that of the control group. The results suggested that hand massage can decrease VAS anxiety, State of anxiety, vital signs and VAS pain of patients who were admitted with ischemic heart disease at cardiac intensive care unit Therefore, It Is proposed that hand massage is an appropriate nursing intervention to relieve anxiety of the patients who were admitted with ischemic heart disease at a cardiac intensive care unit.

Alginate 배양내에서 Transglutaminase가 연골막세포에 미치는 영향

박정선,공차배,김충현,안현정,정미현,성상철,김희중,조현철,이상훈,이명철 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

제주도 토양에서 분리한 가시아메바의 riboprinting에 의한 동정

김현동,전자호,유학선,공현희 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.4

목적 : 가시아메바의 국내 분리주를 확보하고 국내 분포하는 가시아메바의 종류를 파악하며 그 분리주들의 병원성을 간접적으로 평가하기 위해 제주도의 토양에서 분리한 가시아메바 분리주를 riboprinting을 이용하여 종 동정을 하고자 한다. 재료 및 방법 : 제주도의 서로 다른 일곱 장소에서 무균 튜브에 흙을 담아와서 agar plate에서 배양한 후 서로 다른 형태적 특징을 보이는 포낭을 cloning하여 무균배양으로 키웠다. 각각의 핵 DNA를 분리하여 small subunit ribosomal RNA coding DNA 중 일부를 증폭하여 나온 PCR 산물을 8가지 제한효소로 소화시켜 그 RFLP양상을 16비교주와 비교한 후 Nei & Li의 공식에 대입한 후 UPGMA program로 유전적 거리를 구하였다. 결과 : 포낭의 형태는 KA/CS8주를 제외한 7주는 모두 Pussard 및 Pons의 분류에 따른 제 2군에 속하였고 KA/CS8주는 제 3군에 속하였다. 가시아메바 KA/CS1주와 KA/CS2주는 동일한 RELP양상을 나타내었으며 이들은 카스텔라니 가시아메바 Castellani주와 가장 유전적으로 가까왔고, KA/CS3주와 KA/CS6주는 A. triangularis SH621주와, KA/CS4주는 대식가시아메바 Jones주와, KA/CS5주는 대식가시아메바 P23주와. KA/CS7주는 카스텔라니 가시아메바 Neff주와 각각 유전적으로 가장 가까왔고, KA/CS8주는 비교주 중 어느 종과도 유전적으로 가깝지 않았다. 결론 : Acanthamoeba KA/CS1, KA/CS2, KA/CS3, KA/CS4, KA/CS6분리주들은 카스텔라니 가시아메바 complex에 속하였고, KA/CS5, KA/CS7분리주들은 대식가시아메바 complex에 속하였다. In order to collect and identify Acanthamoeba Korean strains. Acanthamoeba strains were isolated from soil of Cheju-do and were identified by comparison of the riboprints with the reference strains. Seven out of eight isolates from soil of Cheju-do belonged to the morphological group Ⅱ (Pussard and Pons, 1977) and the other one belonged to group Ⅲ by cyst morphology. The riboprints of seven isolates belonging to group Ⅱ were compared to those of Acanthamoeba reference strains (11 species 16 strains). KA/CS1, KA/CS2, KA/CS3, KA/CS4 and KA/CS6 strains belonged to A. castellanii complex. KA/CS1 and KA/CS2 strains showed identical riboprints to each other and were the most closely related to A. castellanii Castellani strain. KA/CS3 and KA/CS6 strains were the most closely related to A. triangularis SH621 strain, and KA/CS4 strain to A. polyphaga Jones strain. KA/CS5 and KA/CS7 strains belonged to A. polyphaga complex. KA/CS5 strain was the most closely related to A. polyphaga P23 strain and KA/CS7 strain to A. castellanii Neff strain. KA/CS8 strain belonging to group Ⅲ showed more than 20% estimated sequence divergence from the reference strains (5 species) and made a distinct division in a phenogram reconstructed based on the riboprints.

콘택트렌즈 보존 용기 유래 Acanthamoeba lugdunensis KA/L5주의 내공생세균

정동일,공현희,김태호,황미열,유학선,윤호철,설성용 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

콘택트렌즈 보존 용기 유래 가시아메바 KA/L5주의 세포질 내에 존재하는 bacterial endosymbiont(내공생세균)를 투과전자현미경으로 관찰하여 확인하였다. 숙주인 가시아메바 KA/L5주는 형태학적으로 제2군에 속하였고, rDNA PCR-RFLP 결과 A. lugdunensis로 동정되었다. 미토콘드리아 DNA RFLP와 동위효소 분석상 이 충주는 국내 콘택트렌즈 보존용기에서 가장흔히 분리되는 type인 KA/Ll주, 국내 임상 분리주 중 하나인 KA/E2주, 내공생세균을 가지는 것으로 보고된 병원 냉각수 유래 KA/W4주 및 L3a주와 동일하거나 매우 유사한 성적을 보였다. 내공생세균은 약 1.38× 0.507㎛의 크기였고, 아메바 세포질 내에 불규칙하게 분포하고 있었으며. 그 표면에 아메바의 ribosome이 부착되어 있었다. 내공생세균을 둘러싼 lacunae나 막과 같은 구조는 관찰되지 않았다. Legionella 특이 primer를 이용한 효소중합반응(PCR)에서 내공생세균의 염색체 DNA는 증폭되지 않았다. A. lugdunensis의 우리말 이름을 담수가시아메바로 제안한다. Transmission electron microscopy of an Acanthamieba isolate (KA/L5) from a contact lens case revealed bacterial endosymbionts within cytoplasm of the amoebae, The Acanthamoeba isolate belonged to the morphological group II. Based on the polymerase chain reaction (PCR) - restriction fragment length Polymorphism (RFLP) of 18S ribosomal RNA coding DNA (rDNA), the Isolate was identified as A. lugdunensis. Strain typing by isoenzyme analysis using isoelectric focusing (IEF) and mitochondrial (Mt) DNA RFLP revealed that the isolate was closely related with KA/Ll , the most predominant type of isolates from contact lens storage cases, KA/E2, a clinical isolate, KA/W4, preciously reported to host endosymbionts, and L3a strains of A. lugdunensis. The endosymbionts were similar to those of KA/W4 In aspects that they were randomly distributed in both trophozoites and cysts, and were rod-shaped bacteria measuring approximately 1.38x 0.50㎛. But the number of endosymbionts per amoeba was significantly lower than that of KA/W4. They were neither limited by phagosomal membranes nor included In lacunae- like structure.

감귤 및 오렌지의 소비행태와 선호분석

고성보,강경선,현공남 韓國農業政策學會 1999 농업경영정책연구 Vol.26 No.2

콘택트렌즈 소독제 및 PHMB의 3종 가시아메바 포낭에 대한 살충능력 평가

신종원,유학선,공현희,정동일 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 국내에 시판되고 있는 콘택트렌즈 보존 및 소독액, 그리고 아메바성 각막염의 치료제로서 인정받고 있는 polyhexamethylene biguanide (PHMB)의 가시아메바 포낭에 대한 살충효과를 평가하고자 한다. 대상 및 방법 : 카스텔라니가시아메바 Ma주, 대식가시아메바 Nagington주 및 담수가시아메바 KA/LI주에 대한 소독액의 살충효과의 평가는 한천 plate위에서 수행하였고, 최저살충농도(minimal cysticidal concentration : MCC)의 결정은 microplate를 이용하였다. 7일간 관찰 후, 탈낭하지 않는 가시아메바 포낭은 사멸한 것으로 판정하였다. Chlorhexidine 및 PHMB의 MCC를 결정하기 위해 8시간 및 48시간 처리 실험을 분리하여 시행하였다. 결과 : 시판 소독액 중 chlorhexidine과 thimerosal를 함유하는 용액 C만이 가시아메바 포낭에 대한 살충효과가 있었고, 나머지 3종의 소독액은 살충효과가 없었다. 0.02% PHMB로 4시간 이상 처리한 가시아메바 포낭은 충주에 관계없이 사멸되었다. Chlorhexidine 과 PHMB, 두 약제 모두 8시간 처리 MCC가 48시간 처리 MCC보다 다소 높았다. PHMB에 대한 감수성은 가시아메바 충주에 따라 차이가 격심하였다. 결론 : 3종 가시아메바 포낭에 대한 PHMB의 MCC (0.29∼9.38㎍/㎖)가 chlorhexidine의 그것(2.73∼18.75㎍/㎖)보다 낮아 PHMB는 아메바성 각막염의 치료제 및 콘택트렌즈 소독제로 사용 될 수 있을 것으로 사료되며 화학소독액에 PHMB를 10㎍/㎖ 첨가하는 것이 요망된다. In order to evaluate the cysticidal effects of contact lens disinfectants and polyhexamethylene biguanide(PHMB) against 3 clinical isolates of Acanthamoeba. in vitro cysticidal assay was performed on agar plates covered with heat-inactivated Escherichia coli. Three clinical isolates of Acanthamoeba were A. castellanii Ma strain. A. polyphaga Nagington strain, and A. lugdunensis KA/LI strain. After treatment of Acanthamoeba cysts with the disinfectants or PHMB, the cysts were cultivated for 7 or more days. Cysts which were not excysted in 7 days after treatment were recognized killed. Among commercial disinfectants, the solution C. which contains chlorhexidine and thimerosal. was the only one with cysticidal effects on Acanthamoeba cysts. PHMB of 0.02% concentration was also cysticidal. Sensitivity to PHMB varied profoundly from isolate to isolate. Minimal cysticidal concentration (MCC) of chorhexidine and PHMB were determined by cultivation of cysts treated for 8 or 48 hours with serially diluted chlorhexidine and PHMB in wells of a 96-well microplate containing axenic culture media for amoebae. MCC of 48-hour treatment with both chemicals was lower than that of 8-hour treatment. MCC of PHMB against amoebic cysts was lower than that of chlorhexidine. In the light of these results. PHMB could be a contact lens disinfectant as well as a chemotherapeutic agent of amoebic keratitis.

내시경부비동수술후 비음형대, 스펙트럼 및 비·부비동의 용적변화의 분석

김창수,공수근,이현순,조규섭,왕수건,노환중 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.11

Effect of Exogenous Anti-Mullerian Hormone Treatment on Cryopreserved and Transplanted Mouse Ovaries

Kong, Hyun Sun,Kim, Seul Ki,Lee, Jaewang,Youm, Hye Won,Lee, Jung Ryeol,Suh, Chang Suk,Kim, Seok Hyun SAGE Publications 2016 Reproductive sciences Vol.23 No.1

<P>Follicle loss occurs after ovary cryopreservation and transplantation. To preserve the follicle pool of cryopreserved or grafted ovaries, anti-Mullerian hormone (AMH), which inhibits ovarian follicle recruitment, was used in a mouse model. In experiment 1, ovaries were vitrified warmed with different doses of AMH (0, 5, 15, or 45 g/mL) supplementation. In experiment 2, AMH (0, 50, 250, and 1250 g/mL) was injected into mice before and/or after cryopreserved ovary autotransplantation, and the recipients remained for 7 or 28 days after grafting. Ovaries were evaluated by follicle morphology, density, and apoptosis ratio. Additionally, serum follicle-stimulating hormone was measured in experiment 2. Significantly decreased follicle apoptosis were detected in AMH-treated groups when compared to the control ovaries in experiment 1, meanwhile no positive effect of exogenous AMH was found in experiment 2. Thus, we suggest AMH supplementation during ovary vitrification warming has beneficial effect on reducing follicle apoptosis.</P>

Inhibition of synovial hyperplasia, rheumatoid T cell activation, and experimental arthritis in mice by sulforaphane, a naturally occurring isothiocyanate

Kong, Jin-Sun,Yoo, Seung-Ah,Kim, Hyun-Sook,Kim, Hyun Ah,Yea, Kyungmoo,Ryu, Sung-Ho,Chung, Yeun-Jun,Cho, Chul-Soo,Kim, Wan-Uk Wiley Subscription Services, Inc., A Wiley Company 2010 Vol.62 No.1

<B>Objective</B><P>To investigate whether sulforaphane (SFN), an isothiocyanate derived from cruciferous vegetables such as broccoli, regulates synoviocyte hyperplasia and T cell activation in rheumatoid arthritis (RA).</P><B>Methods</B><P>Synoviocyte survival was assessed by MTT assay. The levels of Bcl-2, Bax, p53, and pAkt were determined by Western blot analysis. Cytokine concentrations in culture supernatants from mononuclear cells were analyzed by enzyme-linked immunosorbent assay. The in vivo effects of SFN were examined in mice with experimentally induced arthritis.</P><B>Results</B><P>SFN induced synoviocyte apoptosis by modulating the expression of Bcl-2/Bax, p53, and pAkt. In addition, nonapoptotic doses of SFN inhibited T cell proliferation and the production of interleukin-17 (IL-17) and tumor necrosis factor α (TNFα) by RA CD4+ T cells stimulated with anti-CD3 antibody. Anti-CD3 antibody–induced increases in the expression of retinoic acid–related orphan receptor γt and T-bet were also repressed by SFN. Moreover, the intraperitoneal administration of SFN to mice suppressed the clinical severity of arthritis induced by injection of type II collagen (CII), the anti-CII antibody levels, and the T cell responses to CII. The production of IL-17, TNFα, IL-6, and interferon-γ by lymph node cells and spleen cells from these mice was markedly reduced by treatment with SFN. Anti-CII antibody–induced arthritis in mice was also alleviated by SFN injection.</P><B>Conclusion</B><P>SFN was found to inhibit synovial hyperplasia, activated T cell proliferation, and the production of IL-17 and TNFα by rheumatoid T cells in vitro. The antiarthritic and immune regulatory effects of SFN, which were confirmed in vivo, suggest that SFN may offer a possible treatment option for RA.</P>

Effects of anti-mullerian hormone supplementation on cryopreserved and transplanted ovarian tissues

Hyun Sun Kong,Jaewang Lee,Eun Jung Kim,Hye Won Youm,Jung Ryeol Lee,Byung Chul Jee,Chang Suk Suh,Seok Hyun Kim 한국발생생물학회 2014 한국발생생물학회 학술발표대회 Vol.2014 No.9

Ovarian tissue cryopreservation and transplantation causes follicle depletion. To overcome this problem, we investigate the effect of Anti-Müllerian hormone (AMH), a follicle recruitment control hormone, supplementation before and/or after mouse ovarian transplantation. A total of 120 5-week-aged BD F-1 female mice were used. The mice were randomly divided into four groups according to AMH doses (0, 5, 25, 125 μg/mL, respectively). AMH was injected intraperitoneally on every other day for a week before, after, or before and after transplantation of ovaries under kidney capsules was performed. One week after transplantation, follicular normality was evaluated by histological analysis and TUNEL assay. In Group A and C, morphologically intact follicle (G1) ratios of AMH treated groups showed no statistically significant difference. In Group B, G1 ratios of 25 and 125 μg/mL of AMH treated groups were higher than those of 5 μg/mL treated group, but there was no improvement in G1 ratio after AMH treatment. In every group, apoptotic follicle ratios did not show any trend according to AMH treatment. Proportions of primordial follicle were not significantly different according to AMH treatment in all groups. The result of the present study demonstrated that AMH treatment during on transplantation of cryopreserved ovaries has no significant effect on follicle survival and prevention of follicle depletion.