The extract of natural plant promotes the proliferation of murine spermatogonial stem cells

Hye-Ryeon Kang,Yong-Hee Kim,Dong-Gu Lee,Ki-Jung Kim,Polash Chandra Karmakar,Hyun-Gu Kang,Sang-Eun Jung,Myeong-Geun Oh,Yeon-Jim Cho,Yu-Ri Choi,Chan Kyu Han,Sanghyung Lee,Buom-Yong Ryu 한국발생생물학회 2015 한국발생생물학회 학술발표대회 Vol.2015 No.9

The aim of this study was to enhance the proliferation efficiency of spermatogonial stem cells (SSCs). In order to improve the proliferation efficiency, we investigated new factors that promote the proliferation of SSCs using in vitro culture method with natural plant extracts. Germ cell populations containing SSCs were collected 6- to 8-days-old from C57BL/6-TG-EGFP (C57GFP) mice and SSCs were isolated from the collected cells via magnetic-activated cell sorting (MACS). Since then, SSCs were cultured for a week with culture medium containing natural plant extracts at concentration of 0.1, 1, and 10 μg/mL. After a week of culture, we looked for an increase, especially a dose-dependent increase, in the number of cells compared to that of the control group. A dose-dependent increase, in the number of cells was observed in the Petasides japonicus-treated groups. Furthermore, we carried out repeated experiment that is process consisting of selection and additional segmentation to explore new factors for activating SSCs at the molecular level. As a results, Petasides japonicus butanol fraction significantly increased the proliferation rate of SSCs in a dose-dependent manner among Petasides japonicus fraction samples. We identified normal expression level of PLZF in SSCs cultured with plant extracts using immunocytochemistry method. Furthermore, we also carried out qRT-PCR and identified normal expression level of Lhx1 and GFRα1. The finding of this study could contribute to improvement of proliferation and activation for SSCs, using culture method with natural plant extracts.

Korean Medical Therapy for Knee Pain after Anterior Cruciate Ligament Reconstruction

Kim, Hye Ryeon,Choi, Yu Na,Kim, Seon Hye,Kang, Ha Ra,Lee, Yoon Joo,Jung, Chan Yung,Cho, Hyun Seok,Kim, Kyung Ho,Kim, Kap Sung,Kim, Eun Jung Korean AcupunctureMoxibustion Medicine Society 2017 대한침구의학회지 Vol.29 No.3

Objectives : The aim of this study was to report the effect of Korean medical therapy on pain and dysfunction after anterior cruciate ligament (ACL) reconstruction. Methods : A 25-year-old man experienced severe pain after right ACL reconstruction surgery. He received Korean medical treatments such as acupuncture, herbal medicine, and physiotherapy from July 10, 2014 to August 2, 2014. Results : After the treatments, his visual analogue scale scores generally decreased and the range of motion of the right knee improved from $0^{\circ}$ to $90^{\circ}$. Furthermore, the Knee Infury and Osteoarthritis Outcome Score increased from 99 to 142. Conclusion : The findings suggest that Korean medical treatments might be effectively used to treat pain and dysfunction after soft-tissue surgeries such as ACL reconstruction. Nevertheless, further research is warranted because of the limited sample size of this study.

북한무용의 변천과 특성에 관한 연구

강혜련(Hye-Ryeon Kang) 한국엔터테인먼트산업학회 2014 한국엔터테인먼트산업학회논문지 Vol.8 No.2

본 논문은 문헌분석접근법에 의해 김일성 및 김정일 시대의 구분을 통한 문화예술정책 기조의 관점에서 북한무용의 변천과정과 특성의 고찰을 연구목적으로 설정하였다. 북한무용에 관련한 문화예술정책과 북한무용 변천에 관한 고찰을 통한 특성을 요약하면 다음과 같다. 첫째, 북한무용은 예술적 향유의 기회 제공이 아닌 정치적 목적성이 부각되는 수단적 가치로 작용하고 있다. 둘째, 북한무용은 혁명성과 투쟁성을 우선하는 주체무용의 원칙 아래 사상성 주입의 도구화로 활용되고 있다. 셋째, 북한무용은 주체무용의 완성이라는 측면에서 혁명적 사상의 계승과 선동선전의 정치성을 수반한 형식적 및 내용적 의미를 갖는다. 넷째, 북한무용은 사회주의 민족문화 건설을 위한 통치체제 유지, 당 정책노선 선전, 그리고 경제건설 선동, 대중의 사상 개조에 중핵적 수단으로 활용되고 있다. 다섯째, 북한무용은 유일지도체제의 정당성과 지도자의 위상 및 우상화를 위한 선전에 중요한 매개체로 작용한다. 여섯째, 북한 무용은 60년대 이후 김일성 주체사상에 따른 주체무용을 확립함을 목적으로 민족적 형식, 사회주의 혁명적 내용, 그리고 인민생활상 반영의 요소가 부각되었던 시기이다. 일곱째, 북한무용은 김정일 시대들어 우리식사회주의 국가통치이념화에 의한 민족성 고취, 생활무용 강조, 무용의 소품화, 당 정책의 선전선동 도구화의 경향성이 나타났다. 여덟 번째, 1960년대 북한무용은 김일성 항일혁명전통의 계승발전과 김일성 유일체계 확립의 수단적 가치를 형상화하는 시기이다. 아홉 번째, 1970년대는 김정일에 의해 북한무용에 소품화, 군무화, 종합화 및 대형화의 경향성이 부각되는 시기이다. 열 번째, 1980년대 북한무용은 발전의 토대가 정비되는 시기와 아울러 대형화가 더욱 부각되는 시기이다. 열한 번째, 1990년대와 2000년대를 지나면서 북한은 정치사회 전반에 중요한 격변기를 통해 북한무용은 당성 강조와 체제선전을 지향하며 새로운 대집단예술체조 형식의 대형화가 가속화 되는 시기로 이해 가능할 것이다. The purpose of this paper is to a study on the A study on the Change and characteristics of the North Korean Dance. In order to achieve the research objectives, including various materials and literature through the study and analysis of how research through literature review was performed. A study on the Change and characteristics of the North Korean Dance through the results are as follows; First, North Korea dance is a strong political purposes. Second, North Korea dance is a Autonomous Idea propaganda tool. Third, North Korea dance form has the political and Content significance. Fourth, North Korea dance is a means of maintaining the system and propaganda policy, economic construction. Fifth, the North Korean leader"s cult dance is a means of propaganda. Sixth, since the 1960s the North Korean dance when the subject is established Autonomous dance. Seventh, North Korea dance is ethnicity, life, policies tend to be propaganda. Eighth, in the 1960s, Kim Il Sung, North Korea"s only dance when the system is established. Ninth, in the 1970s the North Korean military culture, dance, jonghaphwa, enlargement of the time. Tenth, in the 1980s the North Korean dance is a time of heightened further enlargement. The eleventh, a recent North Korean propaganda for the country dance dance performances appear larger.

농특산품 전시판매장 디자인 현황 분석 및 유형별 분석 -농촌관광마을 현장조사 및 농업인 공급자 의식조사를 중심으로-

진혜련 ( Hye Ryeon Jin ),채혜성 ( Hye Sung Chae ),강가혜 ( Ga Hye Kang ),조록환 ( Lok Hwan Jo ) 한국농촌계획학회 2013 농촌계획 Vol.19 No.3

With the increase of visitors to rural tourism villages, the direct selling at sites is getting vitalized. Accordingly, their display stores is getting more important. Therefore, this study has selected 30 domestic rural tourism villages as study objects for the attitude survey of 200 farming suppliers and the site examination for the designs of those display stores in order to analyze their status and classify the types of necessity. Such operation status as sale item, sale method, method of supply and demand, major customer, sales scale, manager, opening hour, and operation cost were examined, to identity and for design factors the pattern, material quality and color were investigated. For the attitude of farming suppliers, the tactics of sales, the reason for being positive or negative, the functionality and the features of display stores were examined through brainstorming. IBM SPSS Statistics 20 Program was employed for Frequency, which indicated that village chiefs and store managers with the sales scale of 1 to 20 million won are dealing with female customers in their 40`s and 50`s and that those stores are open at the time of experience or year round without any operation expense. Permanent type and Fixed type were found to be the design factors of the display-case type with the material and the color of wood and orange respectively. The result of investigation analysis of farming suppliers` attitude showed the followings: the need of display stores is quite high, structure type and permanent type have high fitness and from the viewpoint of display-on-table type as a standard moving type was very convenient. The analysis of significant items at the characteristics of those display stores revealed that their locations, quality conservation, sanitation, users` convenience, designs and promotion are very important. The result of status analysis revealed that though there is a correlation among the types of display stores depending on the visiting season of tourists their installing is not desirable. Three types have been analyzed: Type 1 is a structure type only in the villages with continuous visitors, Type 2 a moving-table type only in the villages with temporary visitors and Type 3 is a fixed display-case type.

류마티스 관절염 환자에서 HLA-DRB1의 유전적 변이와 Etanercept 치료 반응과의 연관성: 예비 연구

윤혜련 ( Hye Ryeon Yun ),강창수 ( Chang Soo Kang ),이경화 ( Kyung Wha Lee ),이혜순 ( Hye Soon Lee ),김태환 ( Tae Hwan Kim ),배상철 ( Sang Cheol Bae ) 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.1

Objective: To investigate the roles of genetic variation in the HLA-DRB1 as predictors of response to etanercept treatment in rheumatoid arthritis (RA) patients. Methods: Clinical responses of 66 patients treated with etanercept were determined according to the ACR criteria (ACR20 and 70). HLA-DRB1 typing and further subtyping of all alleles were performed by polymerase chain reaction, sequence-specific oligonucleotide probe hybridization, and direct DNA sequencing analysis. We tested whether genetic variation in the HLA-DRB1 influenced on the responses to 12 weeks of etanercept therapy. Univariate and multivariate analyses were performed to compare allele and genotype distribution between responders and nonresponders. Results: When allelic association with etanercept response was analyzed with ACR20 and ACR 70 criteria for shared epitope alleles (HLA-DRB1 *0101, *0401, *0404, *0405, *0410, *1001, and *1406 alleles) and protective alleles (HLA-DRB1*0701, *0802, *1301, *1302, *1403, and *1405 alleles), there was no association with etanercept efficacy. When ACR20 nonresponders were compared with ACR70 responders, there was no significant association. Next, we tested genotypic association for shared epitope carriage status. The presence of HLA-DRB1 alleles encoding the shared epitope (1 and 2 copies) was marginally associated with nonresponse effect for ACR 70 response (OR=0.27, 95% CI=0.08∼0.93, P=0.045). Conclusion: There was no influence of genetic variation in the HLA-DRB1 on the response to treatment of RA with etanercept.

진도 씻김굿의 공연학적 재해석

강혜련(Hye-Ryeon Kang),정지현(Ji-Hyun Jung) 한국엔터테인먼트산업학회 2012 한국엔터테인먼트산업학회논문지 Vol.6 No.3

본고는 공연 특성에 있어 상대적으로 소홀히 연구되어 온 진도 씻김굿의 공연학적 특성을 논의하였다. 씻김굿의 진행과 흐름을 공연학적 관점에서 첫 번째는 제의 공간과 제상 및 무구(巫具), 두 번째는 당골의 성무(成巫)과정, 세 번째는 존재(Being)와 의식(Consciousness)의 변환과정에서 당골과 악사의 일시적 변환, 망자(亡者)의 영속적 변환, 상주(喪主)의 일시적?영속적 변환을 굿의 진행과정에 따라 나타나는 공연학적 특성에 관한 논의를 펼치고, 굿의 마지막 순서인 종천과 밤달애를 통해 공연의 관점으로 분석해 보았다. 진도 씻김굿은 공연의 궁극적인 목적인 사회통합, 재충전과 같은 맥락에 있음을 확인하게 되었다. 또한 총체적 예술로서 씻김굿은 공연예술과 구분되어지지 않고, 공연적 속성이 중요시되어지고 있으며, 이런 면에서 공연학적 측면의 의미는 깊다고 할 수 있다. 제의(祭儀)이면서도 총체예술로서 자리매김한 진도 씻김굿은 공연으로서 관객의 요구를 충족시키고 있으며, 죽음을 통해 삶을 반추해 볼 수 있는 계기를 마련해 주는 삶의 끝자락에서 펼쳐지는 축제인 것이다. This study aims to reveal the performance characteristics of J indo Ssitgim Gut (soul-cleansing shamanism originated in Jindo, Korea) as they have relatively been less examined. From the perspective of performance science, this shamanistic ritual was analyzed in four aspects: 1) the ritual space, the sacrificial table, and shaman tools; 2) shaman"s succession; 3) changing processes of beings and consciousness; 4) seeing souls off and night-soothing play. J indo Ssitgim Gut was found to eventually serve for social integrity and spiritual recharge. As a synthetic art, it is not far from a performance art. Rather, the traits of performance science were emphasized a lot. This total art of a sacrificial rite, therefore, naturally meets the desire of the audience. In other words, JSG is a festival giving us an occasion to reflect on life through death.

Long-term outcomes of abdominal paraganglioma

Hye Ryeon Choi,Zeng Yap,Soon Min Choi,Sun Hyung Choi,Jin Kyong Kim,Cho Rok Lee,Jandee Lee,Jong Ju Jeong,Kee-Hyun Nam,Woong Youn Chung,Sang-Wook Kang 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.6

Purpose: Paragangliomas (PGL) are rare neuroendocrine tumors derived from chromaffin cells of the autonomic nervous system. We aim to describe our experience and the long-term outcome of abdominal PGL over the last decade. Methods: A retrospective review of patients diagnosed with PGL in our hospital between November 2005 and June 2017 was conducted. All nonabdominal PGL were excluded and the clinicopathological features and long-term outcomes of the patients were analyzed. Results: A total of 46 patients were diagnosed with abdominal PGL. The average age of diagnosis was 55.4 years and there was no sex predilection. The average tumor size was 5.85 cm and they were predominantly located in the infrarenal position (50%). The mean follow-up period was 42 months (range, 1.8–252 months). All patients with metastases had Pheochromocytoma of the Adrenal Gland Scaled Score (PASS) of ≥4. One patient presented with synchronous metastases while 2 developed local recurrence and distant metastases. One presented with only local recurrence. One patient died 5 years after diagnosis. Conclusion: Abdominal PGL is a rare tumor with excellent long-term prognosis. Recurrence although uncommon, can occur decades after initial diagnosis. Long-term follow-up is therefore recommended for all patients with PGL, especially in patients with PASS of ≥4

The Efficacy of SDH Immunohistochemical(IHC) Stain for the Screening of Hereditary Pheochromocytoma-Paraganglioma(PPGL)

Hye Ryeon Choi,Sang-Wook Kang,Sun Hyung Choi,Soon Min Choi,Jin Kyong Kim,Cho Rok Lee,Jandee Lee,Jong Ju Jeong,Kee-Hyun Nam,Woong Youn Chung,Ja Seung Koo 대한외과학회 2020 대한외과학회 학술대회 초록집 Vol.2020 No.11

Phase II trial of rituximab plus CVP combination chemotherapy for advanced stage marginal zone lymphoma as a first-line therapy: Consortium for Improving Survival of Lymphoma (CISL) study

Kang, Hye Jin,Kim, Won Seog,Kim, Seok Jin,Lee, Je-Jung,Yang, Deok-Hwan,Kim, Jin Seok,Lee, Se-Ryeon,Lee, Gyeong-Won,Kim, Hyo Jung,Kim, Ho Young,Oh, Sung Yong,Kim, Hugh Chul,Eom, Hyeon-Seok,Chung, Joose Springer-Verlag 2012 Annals of hematology Vol.91 No.4

Evaluation of antioxidant activity and total polyphenolic compounds of Polygonum multiflorum Thunb

Hye Ryeon Yang,Seong kyeong Bae,Yunwi Heo,Du Hyeon Hwang,Jae Kuk Byun,Hyeong Jun Yim,Eui kyung Kim,Chang keun Kang 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-