http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
정혜광 ( Hye Gwang Jeong ) 전남대학교 약품개발연구소 2016 약품개발연구지 Vol.25 No.-
The expression of Sirt1, Nrf2 and AhR relates to tumorigenesis, chemoresistance and carcinogenesis. Accumulating evidence suggests that metformin has antitumor activity. This study investigated effects of metformin on expression of Sirt1, Nrf2, AhR and its downstream target genes in cancer cells. Results indicated that metformin down-regulated the constitutive and inducible expression of CYP1A1 and CYP1B1 in MCF-7 and MDA-MB-231 breast cancer cells. Down-regulation of AhR expression was required for metformin-mediated decreases in CYP1A1 and CYP1B1 expression. Metformin inhibited endogenous AhR ligand-induced CYP1A1 and CYP1B1 expression by suppressing TDO expression. Moreover, metformin strongly suppresses HO-1 expression in A549 cancer cells. Metformin also markedly reduced Nrf2 activation and expression. The regulation of Nrf2 expression by metformin is mediated through a Keap1-independent mechanism and that the reduction. of Nrf2 expression is mediated by an attenuation of Raf-ERK signaling by metformin. Additionally, metformin induced p53 protein levels in wild-type p53 cancer cells resulted in up-regulation of miR-34a and down-regulation of Sirt1. Genetic tools demonstrated that the reduction of Sirt1 and Pgc-1α by metformin caused Nrf2 down-regulation via suppression of PPARγ transcriptional activity. The Sirt1 reduction by metformin increased DR5 expression. Metformin pretreatment enhanced the susceptibility of cancer cells to oxidative stress and TRAIL-induced apoptosis. These results demonstrated that metformin down-regulates Sirt1, Nrf2 and AhR expression in cancer cells following with correlative pharmacological activities. Metformin is commonly used for type 2 diabetes. Therefore, metformin may be an effective therapy for the cancer prevention and treatment.
가미청폐화담탕이 천식 유발 병태 모델에서 천식 관련 활성 면역세포에 미치는 영향
임동주,정혜광,이용구,김동희,Lim, Dong-Ju,Jeong, Hye-Gwang,Lee, Yong-Gu,Kim, Dong-Hee 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6
These studies were investigated the effects of Gamicheungpyehwadam-tang (CPHDT) on immune-cell regulation in association with bronchial asthma in OVA-induced mouse model. The administration of 400 mg/kg CPHDT significantly reduced the number of total cells in lung, peripheral lymph node and spleen in OVA-induced bronchial asthma mouse model. The administration of 400 mg/kg CPHDT significantly reduced $CD3^+,{\;}CD19^+$and $CD3^+,{\;}CD69^+$ cell numbers separated from lung, peripheral lymph node and spleen in OVA-induced bronchial asthma mouse model. CPHDT significantly reduced $CD3^+/CCR3^+,{\;}CD4^+,{\;}B220^+/IgE^+$, and $CD3^+/DX5^+$ cell numbers separated from lung, peripheral lymph node and spleen in OVA-induced bronchial asthma mouse model in a dose dependent manner, However, CPHDT significantly reduced $CD8^+$ cell numbers from only lung and spleen. The administration of CPHDT significantly reduced $NK^+$ cell numbers separated from lung of OVA-induced bronchial asthma mouse model in all concentrations, but 200 mg/kg CPHDT reduced $NK^+$ cell numbers separated from peripheral lymph node. These results suggest that CPHDT has anti-asthma and anti-allergy effects. In addition to, CPHDT may be useful treatment of asthma based on the further studies about the individual efficacy search of the components of CPHDT and the adding of variety drugs to CPHDT.