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RISS 인기검색어
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- 저자 : Huining Zhang (검색결과 6 건)
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Antibacterial metabolites from the Actinomycete Streptomyces sp. P294
Huining Su,Hongwei Shao,Keqin Zhang,Guohong Li 한국미생물학회 2016 The journal of microbiology Vol.54 No.2
The Actinomycete strain P294 was isolated from soil and identified as Streptomyces sp. based upon the results of 16S rRNA sequence analysis. Three compounds obtained from the solid fermentation products of this strain have been determined by 1D, 2D NMR and HRMS experiments. These compounds include two new compounds angumycinones C (1) and D (2), and the known compound X-14881 E (3). All compounds were assayed for antibacterial and nematicidal activity. The results showed the three compounds had different degrees of inhibitory activity against several target bacteria but no significant toxicity against the nematode Caenorhabditis elegans.
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Huining Wang,Zixin Zhang,QuanSen Yang,Fangfang Wang,Kang Yan 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.2
Ionic doping is an important approach to modification of crystal structure and physical properties of the piezoelectric ceramics. In this work, the lead-free piezoelectric ceramic (1- x )K 0.18 Na 0.74 Li 0.08 NbO 3 - x AgNbO 3 (abbreviated as KNLN- x AN, 0 ≤ x ≤ 0.8) was prepared by conventional solid-state reaction method. The effect of the Ag doping on the phase structure, dielectric and ferroelectric properties of the KNLN-based ceramics was investigated by the XRD, DSC, dielectric and ferroelectric characterizations. It is found that all KNLN- x AN ceramics show single phase in perovskite structure because the ionic radii of dopant Ag close to the average ionic size at the A-site in KNLN. The KNLN- x AN exhibits the rhombohedral to orthorhombic phase transition at room temperature and the Curie temperature ( T C ) decreases to low temperature with increasing x value. A narrow tetragonal phase region and a wide orthorhombic phase region form for the compositions with x ≥ 0.4, indicating that the Ag doping stabilizes orthorhombic phase in wide temperature for the KNLN. A ferroelectric anomalies due to high electrical field induced phase transition is found in the sample with x = 0.1. When x = 0.4, high Curie temperature ( T C = 380 °C), large remnant polarization ( P r = 24.4 μC/cm 2 ) and high dielectric constant ( ε r = 10,000 at 1 kHz) at T C are achieved.
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Qiangxing Zhang,Jianfeng Liu,Huining Xu,Yin Zeng,Chunping Wang,Lu Wang 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.12
To investigate the gas sealing capacity of limestone as cap rock for gas reservoir at different burial depths, triaxial compression coupled with permeability tests of limestone subjected to different confining pressures and temperatures were carried out using the improved MTS 815 test machine. During the tests, acoustic emission (AE) monitoring system was also employed to estimate the rock damage. Test results demonstrated a degradation of triaxial compression strength with the increase in temperature and decrease of confining pressure. An empirical equation for the peak stress was established considering confining pressure and temperature. Three different permeability evolution patterns of limestone samples can be proposed based on the experimental results under different coupled thermo-hydro-mechanical (THM) conditions. According to the spatial-temporal characteristics of AE signals, permeability evolution was highly related to the fracture growth and connection in limestone. Ultimately, the experimental results revealed that the permeability of limestone gradually decreased with the increasing confining pressure and temperature. In addition, an exponential relationship between initial permeability and confining pressure was established.
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MMP-2-responsive fluorescent nanoprobes for enhanced selectivity of tumor cell uptake and imaging
Sun, Lu,Xie, Shuping,Ji, Xiuru,Zhang, Jingming,Wang, Dongmei,Lee, Seung Jin,Lee, Hyukjin,He, Huining,Yang, Victor C. The Royal Society of Chemistry 2018 Biomaterials Science Vol.6 No.10
<P>It is difficult to develop highly selective substrate-based fluorescent nanoprobes for specific matrix metalloproteinases (MMPs) due to overlapping substrate specificities among the family of MMP enzymes. To resolve this issue, we have developed novel fluorescent nanoprobes that are highly selective for soluble MMP-2. Herein, MMP-2-responsive nanoprobes were prepared by immobilizing fluorescent fusion proteins on nickel ferrite nanoparticles <I>via</I> the His-tag nickel chelation mechanism. The fusion protein consisted of a fluorescent mCherry protein with a cell penetrating peptide (CPP) moiety. An MMP-2 cleavage site was also introduced within the fusion protein, which was directly linked to the nickel ferrite nanoparticles. The selectivity of nanoprobes was modulated by hiding the cleavage site of MMP-2 substrates deeply inside the system, which could result in strong steric hindrance between the nanoprobes and MMPs, especially for membrane-tethered MMPs such as MMP-14. A cell-based assay demonstrated that the nanoprobes could only be activated by tumor cells secreting soluble MMP-2, but not membrane-tethered MMP-14. To further evaluate the contribution of the steric hindrance effect on the nanoprobes, a truncated recombinant MMP-14 was employed to confer their cleavage activity as compared to native membrane-tethered MMP-14. Furthermore, a designed probe with a diminished steric hindrance effect was proved to be activated by membrane-tethered type MMP-14. The results indicated that the design of fluorescent nanoprobes employing the steric hindrance effect can greatly enhance the selectivity of MMP-responsive nanoprobes realizing the specific detection of soluble MMP-2 in a tumor microenvironment. We believe that highly selective MMP-2-responsive fluorescent nanoprobes have broad impacts on biomedical applications including molecular imaging and labeling for tumor detection.</P>
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Aerobic Denitrification: A Review of Important Advances of the Last 30 Years
Bin Ji,Kai Yang,Lei Zhu,Yu Jiang,Hongyu Wang,Jun Zhou,Huining Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Understanding aerobic denitrification has become an important focus of environmental microbiology. Aerobic denitrification can be performed by various genera of microorganisms and describes the use of nitrate (NO3 −) as oxidizing agents under an aerobic atmosphere. Isolation of aerobic denitrifiers, enzymes involved in aerobic denitrifiers, phylogenetic distribution of aerobic denitrifiers, factors affecting the performance of aerobic denitrifiers, attempts of applications and possible future trends are depicted. The periplasmic nitrate reductase is vital for aerobic denitrifiers and NapA gene may be the proof of aerobic denitrification. Phylogenetic analysis revealed that aerobic denitrifiers mainly belong to α-, β- and γ-Proteobacteria. Aerobic denitrifiers tend to work efficiently at 25 ~ 37°C and pH 7 ~ 8, when dissolved oxygen concentration is 3 ~ 5 mg/L and C/N load ratio is 5 ~ 10. In addition, recent progresses and applications on aerobic denitrifiers are described, including single aerobic reactors, sequencing batch reactor and biofilm reactors. The review attempts to shed light on the fundamental understanding in aerobic denitrification.
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