Mitochondrial citrate accumulation drives alveolar epithelial cell necroptosis in lipopolysaccharide-induced acute lung injury

Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.

Damping enhancement of the inerter on the viscous damper in mitigating cable vibrations

Hui Gao,Hao Wang,Jian Li,Zhi Hao Wang,Youhao Ni,Ruijun Liang 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.1

This paper systematically investigates the effect of the inerter on the damping enhancement of a cable with a viscous damper (VD) installed close to the cable end. Three cases are considered, including the inerter installed parallel with the VD (PVID), the inerter placed in series with the VD (SVID), and the inerter installed at a higher location of the VD (HVID). The asymptotic solutions of the three cases are derived, which can predict the cable modal damping ratio when the inerter and the VD cause minimal perturbation in the undamped frequency of the cable. The effect of the inerter on the modal behavior of the cable with the VD is investigated. Based on the constrained static output LQR method, the effects of the inerter on the damping enhancement of the VD in mitigating cable multi-mode vibrations are further evaluated. The results show that the inerter can improve the control performance of the VD when the inertance is less than the optimum value. Further increasing the inertance beyond the optimum value, the optimum modal damping ratio of the cable decreases, and mode crossover is observed for the cable with PVID and HVID. Compared with the case where the VD and the inerter are located at the same location, the case of the HVID is more effective in mitigating cable multi-mode vibrations.

Legal Framework of Compensation for Vessel-Source Oil Pollution Damage in China

Hao, Hui-Juan,Chung, Dae 한중법학회 2017 中國法硏究 Vol.30 No.-

중국은 세계 2위의 원유 수입국이라고 할 수 있다. 원유에 대한 수요가 증가함에 따라서 선박으로 인한 유류오염의 리스크가 증가하고 있는 실정이라고 할 수 있다. 선박으로 인한 유류오염손해에 대한 배상제도는 희생자와 해양환경을 보호하는 중요한 수단이다. 중국은 1992년 유류오염손해에 대한 민사책임에 관한 국제협약(CLC of 1992)에 가입하였고, 2009년에는 선박연료유협약(Bunker Convention)이 발효되었다. 중국은 선박기인 유류오염사건에 대처하기 위하여 배상제도에 대한 국내 입법을 지속적으로 추진해 오고 있다. 예를 들면, 2010년 선박기인 해양오염의 예방 및 통제에 관한 규정을 개정하였고, 이와 함께 선박기인 유류오염의 민사책임에 관한 보험가입 규정이 마련되었다. 그런데 중국의 선박기인 유류오염손해에 대한 배상책임제도는 다음과 같은 몇 가지 문제점이 있다. 첫째, 민사적 구제수단보다 행정적 구제수단이 우월하다는 점이다. 둘째, 유류오염손해배상제도의 내용이 추상적이라는 점이다. 셋째, 유류오염손해배상제도의 기본적 내용의 일부가 부재하다는 점이다. 넷째, 유류오염손해배상제도의 관련 기준이 합리적이지 않다는 점이다. 본 논문의 결론으로서 첫째, 유류오염손해배상의 책임의 주체를 구체적으로 규정할 필요가 있다고 본다. 둘째, 유류오염손해배상의 범위를 보다 구체적으로 규정할 필요가 있다고 생각한다. 셋째, 1969년 유류오염손해에 대한 민사책임에 관한 국제협약(CLC of 1969)을 책임제한과 관련하여 국내 유류오염사건에 적용하는 것은 배제하여야 한다고 본다. As the world’s second largest importer of crude oil, China imported more than 335 million tons of crude oil in 2015. However, along with the growing need for crude oil, the risk of oil pollution from ships has also been increasing. Compensation for vessel-source oil pollution is important for protecting the interests of victims as well as the marine environment. China has acceded to the 1992 CLC and the Bunkers Convention. China has not yet formed a complete legal system of civil compensation for oil pollution. The contents of the relevant parties are mainly stipulated in the principles and procedures. It is difficult to coordinate many problems. The construction of laws and regulations lacks rigorous system support. China clearly needs to establish the system of oil pollution damage compensation. This system should include such aspects as follows. First, the subject of liability in the legal relationship of the oil pollution damage compensation should be defined specifically. Second, in respect of scope of compensation, it is necessary to include the costs of cleaning up and the loss and damage caused by pollution because China's current laws and regulations do not make provisions for the scope of compensation for oil pollution damage caused by ships. Third, in respect of limitation of liability, China should be make a new standard to deal with non-foreign oil pollution cases because the 1969 CLC provisions of limitation of liability is too high.

Impact of cable sag on the efficiency of an inertial mass damper in controlling stay cable vibrations

Zhi-hao Wang,Hui Gao,Yan-wei Xu,Zheng-qing Chen,Hao Wang 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.24 No.1

Passive negative stiffness dampers (NSDs) that possess superior energy dissipation abilities, have been proved to be more efficient than commonly adopted passive viscous dampers in controlling stay cable vibrations. Recently, inertial mass dampers (IMDs) have attracted extensive attentions since their properties are similar to NSDs. It has been theoretically predicted that superior supplemental damping can be generated for a taut cable with an IMD. This paper aims to theoretically investigate the impact of the cable sag on the efficiency of an IMD in controlling stay cable vibrations, and experimentally validate superior vibration mitigation performance of the IMD. Both the numerical and asymptotic solutions were obtained for an inclined sag cable with an IMD installed close to the cable end. Based on the asymptotic solution, the cable attainable maximum modal damping ratio and the corresponding optimal damping coefficient of the IMD were derived for a given inertial mass. An electromagnetic IMD (EIMD) with adjustable inertial mass was developed to investigate the effects of inertial mass and cable sag on the vibration mitigation performance of two model cables with different sags through series of first modal free vibration tests. The results show that the sag generally reduces the attainable first modal damping ratio of the cable with a passive viscous damper, while tends to increase the cable maximum attainable modal damping ratio provided by the IMD. The cable sag also decreases the optimum damping coefficient of the IMD when the inertial mass is less than its optimal value. The theoretically predicted first modal damping ratio of the cable with an IMD, taking into account the sag generally, agrees well with that identified from experimental results, while it will be significantly overestimated with a taut-cable model, especially for the cable with large sag.

Isolation, Identification, and Performance Studies of a Novel Paraffin-degrading Bacterium of Gordonia amicalis LH3

Dong-Hui Hao,Xin Song,Jian-Qiang Lin,Yu-Jie Su,Yin-Bo Qu,Jian-Qun Lin 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.1

In this study, we describe the isolation and identification of a novel long-chain n-alkane degrading strain, Gordonia amicalis LH3. Under aerobic conditions, it utilized approximately 18.0% of paraffin (2% w/v) after 10 day of incubation, and the paraffin compositions of C18C24 alkalines were utilized preferentially. Under anaerobic conditions, paraffin utilization was approximately 1/8 that seen under aerobic conditions, and the compositions of C34 and C36 alkalines were utilized preferentially. The effects of salinity, temperature, and biosurfactants on paraffin degradation were also evaluated. The strain was also demonstrated to grow on oil, and decreased oil viscosity by 44.7% and degraded oil by 10.4% under aerobic conditions. Our results indicated that G. amicalis LH3 has potential applications in paraffin control, microbial enhanced oil recovery (MEOR), and the bioremediation of hydrocarbon-polluted environments.

Study on the Wind-induced Interference Effect of a Large-span Roof Structure Building Group

Yong Hao,Tian-jiao Han,Hang-fei Wu,Hua-hai Dong,Yin Zhou,Chun-hui Du,Yun-hui Han 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.4

The wind-induced interference effects existing during the construction of a building group were studied in the context of the Zhangjiakou Olympic Sports Center project. Based on CFD numerical simulations, 120 and 96 different working conditions during construction of a swimming pool and speed skating hall were simulated and analyzed respectively, and the numerical simulation results were found to be in high agreement with the wind tunnel test results, which verified the accuracy and feasibility of the numerical simulation. By comparing the wind-induced interference effects of the surrounding buildings on the swimming pool and the speed skating hall during different construction periods, it was found that the swimming pool was more affected by the interference of the surrounding buildings than the speed skating hall, and the wall area was more affected by the interference than the roof area. The wind-induced response of the swimming pool structure during different construction periods was analyzed, and it was found that the interference effect under some working conditions had a greater impact on the structural stress, and it was suggested that the wind interference effect brought about by the construction of the building should not be ignored in such projects.

Control of a Magnetic Levitation System via Feedback Error Learning

Shuang-Hui Hao,Zi-Jiang Yang,Teruo Tsuji 제어로봇시스템학회 1993 제어로봇시스템학회 국내학술대회 논문집 Vol.1 No.2

The miR-98-3p/JAG1/Notch1 axis mediates the multigenerational inheritance of osteopenia caused by maternal dexamethasone exposure in female rat offspring

Han Hui,Xiao Hao,Wu Zhixin,Liu Liang,Chen Ming,Gu Hanwen,Wang Hui,Chen Liaobin 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

As a synthetic glucocorticoid, dexamethasone is widely used to treat potential premature delivery and related diseases. Our previous studies have shown that prenatal dexamethasone exposure (PDE) can cause bone dysplasia and susceptibility to osteoporosis in female rat offspring. However, whether the effect of PDE on bone development can be extended to the third generation (F3 generation) and its multigenerational mechanism of inheritance have not been reported. In this study, we found that PDE delayed fetal bone development and reduced adult bone mass in female rat offspring of the F1 generation, and this effect of low bone mass caused by PDE even continued to the F2 and F3 generations. Furthermore, we found that PDE increases the expression of miR-98-3p but decreases JAG1/Notch1 signaling in the bone tissue of female fetal rats. Moreover, the expression changes of miR-98-3p/JAG1/Notch1 caused by PDE continued from the F1 to F3 adult offspring. Furthermore, the expression levels of miR-98-3p in oocytes of the F1 and F2 generations were increased. We also confirmed that dexamethasone upregulates the expression of miR-98-3p in vitro and shows targeted inhibition of JAG1/Notch1 signaling, leading to poor osteogenic differentiation of bone marrow mesenchymal stem cells. In conclusion, maternal dexamethasone exposure caused low bone mass in female rat offspring with a multigenerational inheritance effect, the mechanism of which is related to the inhibition of JAG1/Notch1 signaling caused by the continuous upregulation of miR-98-3p expression in bone tissues transmitted by F2 and F3 oocytes.

Study on the Seismic Optimization Scheme of Steel Bundled-Tube Structure with Vertical Setback

Yong Hao,Feng Chen,Yun-hui Han,Xue-qian Hao,Chun-hui Du,Qiu-yu Ding 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

In order to improve the seismic performance of vertical setback steel bundled-tube structure and meet the dynamic multi-objective requirements of seismic performance-based design, the optimization effects under rare earthquakes on the zone of fortification intensity 7 and 8 in China are researched by improving members stiffness and adding dampers in the weak parts of structure, based on dynamic elastic-plastic analysis. The results show that: 1) Under rare earthquakes on the zone of fortification intensity 7, increasing the stiffness of weak skirt beam can improve the seismic performance of spiral and symmetrical setback structure; 2) For the spiral setback structure, under rare earthquakes on the zone of fortification intensity 7, the damper can only be arranged at the junction of the first layer of the setback frame unit and the frame unit without setback. However, under rare earthquakes on the zone of fortification intensity 8, it is not advisable to install dampers at the weak areas; 3) After incorporating dampers at the weak areas of symmetrical setback structure, the optimized members experienced a reduction in stress levels, significantly decreased the inter-layer displacement angles with a maximum reduction of 43%, and the optimization effect is significant.

Regulatory Role of SFN Gene in Hepatocellular Carcinoma and Its Mechanism

Ying Hui,Hao Zeng,Yi Feng,Wenzhou Qin,Peisheng Chen,Lifang Huang,Wenfu Zhong,Liwen Lin,Hui Lv,Xue Qin 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.3

Purpose: This study aims to explore the differential expression of SFN gene and its regulatory role in different hepatocarcinoma cells, and the impact on hepatocarcinoma. Materials and Methods: High and low SFN expression cells were screened by qRT-PCR and western blotting methods. SFN over expression and interference vectors were constructed. Cell viability was detected by CCK8 kit, cell cycle and apoptosis were detected by flow cytometry. Cell invasion and migration were detected. CCNB1 and CDK1 expression levels were detected by qRT-PCR and Western blotting methods. Results: The high SFN expression BEL7402 cells and the low SFN expression Hep3B cells were screened from Hep3B, HepG2, and BEL7402 cells. The activity of Hep3B cells overexpression vector SFNpcDNA3.1(+) decreased and apoptosis increased, the ratio of G0/G1 decreased and the ratio of S phase increased. The activity of BEL7402 cells transfected with SFN siRNA decreased and apoptosis increased, the ratio of G0/G1 decreased and the ratio of G2/M increased. Interference and overexpression vectors have little effect on the invasion and migration of the two cells. The expression of CDK1 in Hep3B cells decreased significantly, the expression of CDK1 and CCNB1 in BEL7402 cells increased significantly. Conclusions: The differentially expressed SFN gene can regulate the growth of the two hepatocarcinoma cells, high expression of SFN gene can inhibit their growth. The mechanism may be achieved by regulating CCNB1 and CDK1 expression.