http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Fang Jianming,Huang Jianxin,Zhang Xiaodong 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.4
Fibronectin 1-derived circular RNA hsa_circ_0058092 is a novel potential oncogene in gastric cancer (GC). Yet, previous studies have not determined the role of hsa_circ_0058092 GC progression and the underlying mechanism. Herein, we investigated its role and competing endogenous RNA (ceRNA) pathway in the development of GC. The results showed that hsa_circ_0058092 was substantially upregulated in GC patients’ tissues and cells, allied with upregulated SIX1 and downregulated miR-1294 compared with normal gastric tissues and cells. There were linear correlations among hsa_circ_0058092, miR-1294 and SIX1 levels in GC tumors. Moreover, hsa_circ_0058092 acted as a miR-1294 sponge, and miR-1294 targeted SIX1. Functionally, colony formation, EdU positive rate, tumor growth of GC cells, as well as ki-67 expression in xenograft tumors was greatly suppressed by depressing hsa_circ_0058092. Besides, hsa_circ_0058092 knockdown repressed GC cell migration and invasion, accompanied with increased E-cadherin expression and descended N-cadherin expression. Moreover, inhibiting miR-1294 expression could counteract hsa_circ_0058092 knockdown-mediated effects in GC cells. The inhibitory effects of miR-1294 mimics on GC cell malignancy were relieved by increasing SIX1 expression. Further, hsa_circ_0058092 depletion repressed SIX1 protein expression by interacting with miR-1294. Hsa_circ_0058092 was oncogenic in GC cell proliferation and motility via ceRNA pathway of hsa_circ_0058092/miR-1294/SIX1.
( Suxia Bao ),( Jianming Zheng ),( Ning Li ),( Chong Huang ),( Mingquan Chen ),( Qi Cheng ),( Kangkang Yu ),( Shengshen Chen ),( Mengqi Zhu ),( Guangfeng Shi ) 대한간학회 2017 Gut and Liver Vol.11 No.6
Background/Aims: To investigate the role of selected serum microRNA (miRNA) levels as potential noninvasive biomarkers for differentiating S0-S2 (early fibrosis) from S3-S4 (late fibrosis) in patients with a chronic hepatitis B virus (HBV) infection. Methods: One hundred twenty-three treatment-naive patients with a chronic HBV infection who underwent a liver biopsy were enrolled in this study. The levels of selected miRNAs were measured using a real-time quantitative polymerase chain reaction assay. A logistic regression analysis was performed to assess factors associated with fibrosis progression. Receiver operating characteristic (ROC) curve and discriminant analyses validated these the ability of these predicted variables to discriminate S0-S2 from S3-S4. Results: Serum miR-29, miR-143, miR-223, miR-21, and miR-374 levels were significantly downregulated as fibrosis progressed from S0-S2 to S3-S4 (p<0.05), but not miR-16. The multivariate logistic regression analysis identified a panel of three miRNAs and platelets that were associated with a high diagnostic accuracy in discriminating S0-S2 from S3-S4, with an area under the curve of 0.936. Conclusions: The levels of the studied miRNAs, with the exception of miR-16, varied with fibrosis progression. A panel was identified that was capable of discriminating S0-S2 from S3-S4, indicating that serum miRNA levels could serve as a potential noninvasive biomarker of fibrosis progression. (Gut Liver 2017;11:860-869)
Tian, Fengyuan,Huang, Shuo,Xu, Wangda,Chen, Lan,Su, Jianming,Ni, Haixiang,Feng, Xiaohong,Chen, Jie,Wang, Xi,Huang, Qi The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6
Background: Incretin impairment, characterized by insufficient secretion of L-cell-derived glucagon-like peptide-1 (GLP-1), is a defining step of type 2 diabetes mellitus (T2DM). Ginsenoside compound K (CK) can stimulate GLP-1 secretion; however, the potential mechanism underlying this effect has not been established. Methods: CK (40 mg/kg) was administered orally to male db/db mice for 4 weeks. The body weight, oral glucose tolerance, GLP-1 secretion, gut microbiota sequencing, bile acid (BA) profiles, and BA synthesis markers of each subject were then analyzed. Moreover, TGR5 expression was evaluated by immunoblotting and immunofluorescence, and L-cell lineage markers involved in L-cell abundance were analyzed. Results: CK ameliorated obesity and impaired glucose tolerance in db/db mice by altering the gut microbiota, especially Ruminococcaceae family, and this changed microbe was positively correlated with secondary BA synthesis. Additionally, CK treatment resulted in the up-regulation of CYP7B1 and CYP27A1 and the down-regulation of CYP8B1, thereby shifting BA biosynthesis from the classical pathway to the alternative pathway. CK altered the BA pool by mainly increasing LCA and DCA. Furthermore, CK induced L-cell number expansion leading to enhanced GLP-1 release through TGR5 activation. These increases were supported by the upregulation of genes governing GLP-1 secretion and L-cell differentiation. Conclusions: The results indicate that CK improves glucose homeostasis by increasing L-cell numbers, which enhances GLP-1 release through a mechanism partially mediated by the gut microbiota-BA-TGR5 pathway. Therefore, that therapeutic attempts with CK might be useful for patients with T2DM.
Modeling of Piezoelectric Langevin Transducers by Using Mixed Transfer Matrix Methods
Bo Fu,Chao Li,Jianming Zhang,Zhenwei Huang,Tobias Hemsel 한국물리학회 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.57 No.41
In the modeling of piezoelectric Langevin transducers using the usual transfer matrix methods, some simplifications have been adopted. This leads to a reduction in the model quality. A mixed transfer matrix method is employed in the modeling of Langevin transducers, where the pre-stressed bolt is modeled as a separate four-pole element connected to other elements in parallel. Based on the mixed transfer matrix method, the four (six)-pole element description of the piezoelectric Langevin transducer is built up, and the total transfer matrix relation is derived. The resonance frequencies of the transducer are calculated and then measured using an impedance analyzer (HP4192). Experimental results show that the mixed transfer matrix method has better modeling quality than the usual transfer matrix method for the vibration analysis of piezoelectric Langevin transducers.
Shulin Cai,Shuaishuai Hu,Jianming Wu,An Huang,Lihong Geng,Xiangfang Peng 한국섬유공학회 2022 Fibers and polymers Vol.23 No.5
Cellulose-based fluorescent fibers were successfully fabricated by interfacial polyelectrolyte complexation (IPC)spinning, in which thioglycolic acid-modified CdTe quantum dots (QDs) were incorporated. The fibers exhibited differentfluorescent colors depending on different sizes of CdTe QDs. The IPC spinning process based on the progressive selfassemblyof negatively charged cellulose nanofibers (CNFs) and positively charged chitosan endowed the fibers highorientation degree. Together with the superior mechanical property of CNFs and the strong interfacial ionic bond andhydrogen bond interaction between CNFs and chitosan, the fluorescent fibers showed a high tensile strength above 689 MPaand toughness above 25 MJ/m3, which provided a guarantee for weaving the fluorescent fibers into textiles. With excellentoptical and mechanical performance, the resulting fluorescent fibers showed a promising potential as flexible wearable anticounterfeitingdevices, which the hard-to-see tag woven using the fluorescent fibers appeared clearly under 365 nmultraviolet light illumination. This paper would provide a new avenue for the preparation of high-performance environmentfriendlyfluorescent anti-counterfeiting textiles.