직장 카르시노이드 종양에서 Ki-67 발현의 예후적 중요성

홍수민 ( Su Min Hong ),김유선 ( You Sun Kim ),문정섭 ( Jung Seop Moon ),김진남 ( Jin Nam Kim ),오명기 ( Myoung Ki Oh ),권선옥 ( Sun Ok Kwon ),정성연 ( Seong Yeon Jeong ),홍성우 ( Seong Woo Hong ),강윤경 ( Yun Kyung Kang ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Background/Aims: Rectal carcinoid tumors can be resected with endoscopy, and it is important to assess their prognostic factors. We evaluated the potential of Ki-67 expression as a prognostic factor in rectal carcinoid tumors. Methods: We retrospectively reviewed the medical records of 37 patients with rectal carcinoid tumors who got endoscopic resection from January 2001 to January 2011 at Inje University Seoul Paik Hospital. We analyzed their endoscopic and histologic findings, Ki-67 expression, clinical outcome, and prognosis. Results: The mean age (±SD) of the patients was 56.3±10.7 years, and the male : female ratio was 3.6:1. The mean tumor size was 0.5±0.4 cm, 33 patients showed grade 1 tumors (89.2%) and the average Ki-67 expression was 0.7±1.2%. Thirty five patients underwent endoscopic mucosal resection, and two required endoscopic submucosal dissection. Eight patients had positive margins after resection, but no cases of lymphovascular invasion were identified. The median follow-up duration was 21.4±25.4 months, and no recurrences were observed. Conclusions: In low grade rectal carcinoid tumors which are lack of central depression on colonoscopy, the expression of a molecular marker of malignant potential, Ki-67, was low. Therefore, endoscopic resection seemed to be a safe and effective treatment for these tumors.

Potent neutralization of vacuolating cytotoxin (VacA) of Helicobacter pylori by immunoglobulins against the soluble recombinant VacA.

Ki, Mi-Ran,Hong, Il-Hwa,Park, Jin-Kyu,Hong, Kyung-Sook,Hwang, Ok-Kyung,Han, Jung-Yuan,Ji, Ae-Ri,Park, Se-Il,Lee, Seung-Keun,Yoo, Sung-Eun,Jeong, Kyu-Shik Potamitis Press 2009 Anticancer research Vol.29 No.6

<P>BACKGROUND: The recombinant vacuolating cytotoxin (rVacA) of Helicobacter pylori that retains native conformational epitopes was evaluated as a vaccine antigen for anti-H. pylori treatment. METHODS: s1m1 vacA gene fraction encoding the mature VacA protein was expressed as a soluble protein in E. coli at low temperature. The efficacy of anti-rVacA antibody against VacA or H. pylori was assessed in vitro using AGS cells and in vivo using a murine model. RESULTS: The rabbit antisera against rVacA completely neutralized the vacuolating activity and partially inhibited the cell death induced by VacA in AGS cells. Oral immunization of C57BL/6 mice with rVacA plus CpG-oligodeoxynucleotide (ODN) as an ajuvant stimulated specific anti-VacA antibody and mucosal immune responses which correlated with decreased systemic immune responses and gastric urease activities (p>0.05). CONCLUSION: The rVacA antigen possessing conformational epitopes may have potential as a vaccine component and may be useful in serological and histopathological analysis.</P>

Effect of Propranolol on Portal Pressure and Systemic Hemodynamics in Patients with Liver Cirrhosis and Portal Hypertension: A Prospective Study

( Ki Tae Suk ),( Moon Young Kim ),( Dong Hun Park ),( Kyu Hong Kim ),( Ki Won Jo ),( Jin Hon Hong ),( Jae Woo Kim ),( Hyun Soo Kim ),( Sang Ok Kwon ),( Soon Koo Baik ) 대한소화기기능성질환·운동학회 2007 Gut and Liver Vol.1 No.2

Background/Aims: Propranolol can prevent variceal bleeding by ameliorating portal hypertension. We conducted this study to determine the effect of propranolol on portal hypertension and the optimal required dose in Korean cirrhotic patients. Methods: This study prospectively evaluated 50 patients with cirrhosis who exhibited variceal bleeding. The hepatic venous pressure gradient (HVPG), portal venous flow, heart rate (HR), and blood pressure were assessed both at baseline and at 3 months after the treatment. The initial dose of propranolol (20 mg) was subsequently adjusted until the target HR was reached. Patients in whom HVPG reduced by >20% or to less than 12 mmHg were defined as responders. Results: Propranolol significantly (p<0.01) reduced the HVPG (-21±26%, mean±standard deviation), portal venous flow (-25±21%), HR (-20±13%), and blood pressure (-3±13%). Twenty-nine patients were responders, and the optimal required dose was 154.4 mg. The main complication was dizziness (24%), but this was not serious enough to require medication withdrawal. Conclusions: Propranolol is safe and effective at reducing portal pressure in Korean cirrhotic patients. An effective improvement in portal hypertension requires the dose to be increased until the target HR is reached. (Gut and Liver 2007;1:159-164)

A family with axonal sensorimotor polyneuropathy with TUBB3 mutation

HONG, YOUNG BIN,LEE, JA HYUN,PARK, HYUNG JUN,CHOI, YU-RI,HYUN, YOUNG SE,PARK, JI HOON,KOO, HEASOO,CHUNG, KI WHA,CHOI, BYUNG-OK SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.11 No.4

<P>Mutations in the 관???tubulin isotype III (TUBB3) gene result in TUBB3 syndrome that includes congenital fibrosis of the extraocular muscle type 3 (CFEOM3), intellectual impairments and/or an axonal sensorimotor neuropathy. In the present study, a TUBB3 D417N mutation was identified in a family with axonal sensorimotor polyneuropathy by whole exome sequencing. The proband exhibited gait disturbance at the age of 12 years and was wheelchair bound at 40 years. However, the proband's cousin exhibited gait disabilities at 45 years of age and was still able to walk when he was 60 years old. Ophthalmoplegia and intellectual impairment were not observed in either patient. A sural nerve biopsy identified an absence of large myelinated fibers without demyelinating degeneration. Based on these clinical features, the two patients exhibited an axonal peripheral neuropathy without CFEOM3. These results therefore suggested that certain TUBB3 mutations may predominantly be associated with axonal peripheral neuropathy. Furthermore, the results also suggested that TUBB3 mutations may be implicated in modulating the inter??? and intra???familial heterogeneity of clinical phenotypes.</P>

Identification of causative mutations in patients with Leigh syndrome and MERRF by mitochondrial DNA-targeted next-generation sequencing

Hong, Hyun Dae,Kim, Eunja,Nam, Soo Hyun,Yoo, Da Hye,Suh, Bum Chun,Choi, Byung-Ok,Chung, Ki Wha Korean Society of Medical Genetics and Genomics 2015 대한의학유전학회지 Vol.12 No.2

Purpose: Mitochondrial diseases are clinically and genetically heterogeneous disorders, which make their exact diagnosis and classification difficult. The purpose of this study was to identify pathogenic mitochondrial DNA (mtDNA) mutations in 2 Korean families with myoclonic epilepsy with ragged-red fibers (MERRF) and Leigh syndrome, respectively. Materials and Methods: Whole mtDNAs were sequenced by the method of mtDNA-targeted next-generation sequencing (NGS). Results: Two causative mtDNA mutations were identified from the NGS data. An m.8344A>G mutation in the tRNA-Lys gene (MT-TK) was detected in a MERRF patient (family ID: MT132), and an m.9176T>C (p.Leu217Pro) mutation in the mitochondrial ATP6 gene (MT-ATP6) was detected in a Leigh syndrome patient (family ID: MT130). Both mutations, which have been reported several times before in affected individuals, were not found in the control samples. Conclusion: This study suggests that mtDNA-targeted NGS will be helpful for the molecular diagnosis of genetically heterogeneous mitochondrial diseases with complex phenotypes.

A Study on Symbolism of Graffiti

Hong, Ki-hyeon,Lee, Choon-hee,Shin, Sang-ok The Costume Culture Association Department of Clot 1998 Fashion, industry and education Vol.1 No.1

In the postmodern society, graffiti style involves various symbolism according to interpreters because of different ways of decoding signs. This study analyzes the graffiti style as symbolism of nonlinguistic communication media and examines variety, phenomenon and symbolisms of graffiti on their casual attire from the point of semiological view and refers to many graffiti works in each country. As a result. German and korean graffiti-figured costumes have common signifiance as follows. First, they induce the application of DIY technique, expand awareness of participation, and lead tired-of mass-production consumers to the role of positive sign-producer. Second, they notify participation in environmental protection. Third, they stress a leisure activity with the pleasure of making something. Fourth, they premise that even the same text can produce various signifie according to context, and be interpreted and communicated by receivers. Fifth, unlike previous graffiti which had protestation as humor and wit shown in figures and colors, it tells that the sign is changed according to time and space.

Frequency and impacts of chronic cough or sputum in COPD

( Ki Hyun Kim ),( Tae Hee Kim ),( Jin Sun Jang ),( Yu Il Kim ),( Hyung Joo Oh ),( Ha Young Park ),( Tae Ok Kim ),( Chul Kyu Park ),( Jung Hwan Lim ),( Hong Jun Shin ),( Hee Jung Ban ),( In Jae Oh ),( 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-

BACKGROUND: Chronic cough or sputum is a detrimental symptom that is associated with disease progression, i.e. FEV1 decline in COPD. However, effective treatments for cough or sputum have not yet been identified and there are very little data on their frequency and impacts on quality of life in COPD. Methods: A cross-sectional survey was planned in the general population of Korea. This survey collected information on the Korea National Health and Nutrition Examination Survey V, from 2010 to 2012. Chronic cough or sputum was defined a history of persistent cough or sputum more than 3months per year. Results: A total of 1,265 COPD subjects aged 40 years or more were enrolled in this study. The prevalence of chronic cough or sputum in COPD was 14.4 % (n=183/1,264). The COPD patients with cough or sputum had lower FEV1%pred than the patients without symptoms (FEV1%pred= 74.2% vs 79.3%, p<0.001). . Also, a poorer quality of life was observed in the patients with chronic cough or sputum (EQ-5D index = 0.89 vs 0.92, p=0.024). Conclusions: Chronic cough or sputum occurs infrequently in adults with stable COPD. However, this should be considered as an important symptom that can be associated with lung function and quality of life in COPD. Those are needed to be a therapeutic target in COPD.

Determination of Acaricides in Honey by Solid-Phase Extraction and Gas Chromatography / Mass Spectrometry

Hong, Joo-Yeon,Jung, Ok-Sang,Ryoo, Jae-Jeong,Hong, Jong-Ki Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.1

An analytical method based on solid-phase extraction and gas chromatography / mass spectrometry has been developed for measurement of acaricides (amitraz, bromopropylate, coumaphos, cymiazole, and tetradifon) in honey sample. In the stability test of acaricides in honey, amitraz underwent a rapid degradation into 2,4-dimethylaniline (DMA), 2,4-dimethylphenylformamide (DMPF), and N-(2,4-dimethylphenyl)-N'-methylformamidine (DMPMF), whileas other acaricides were found to be stable even for over three months. Extraction of five acaricides from 5g of honey sample was carried out by liquid-liquid extraction using 20mL of ethylacetate. For purification, Florisil-SPE cartridge with elution of 5mL of n-hexane/ acetone (55:45, v/v) was found to remove interferences effectively. Quantification was performed using gas chromatography / mass spectrometry in the selected ion monitoring mode. Spiking experiments were carried out to determine the recovery, precision, and limits of detection (LODs) of the method. The overall recovery values from honey spiked at 0.02 and 0.20 ${\mu}g/g$ levels, respectively, were found to be greater than 75% for all acaricides. The method detection limits for acaricides were ranged from 0.1 to 3 ppb. The developed method in this study was applied for the monitoring of acaricides in honey products collected from urban markets in Korea.

Development of a Land Surface Temperature-Retrieval Algorithm from MTSAT-1R Data

Ki-Ok Hong,Myoung-Seok Suh,Jeon-Ho Kang 한국기상학회 2009 Asia-Pacific Journal of Atmospheric Sciences Vol.45 No.4

Land surface temperature (LST) is a useful surface parameter for a wide range of applications, such as agriculture and the numerical and climate-modeling community. As a result, operational observations of LST on various scales are gaining increased scientific interest. In this study, we developed a split window-type LST retrieval algorithm to estimate the LST from MTSAT-1R data. The coefficients of the split-window algorithm were obtained by means of a statistical regression analysis using a synthetic match-up database. The database was made through radiative transfer simulations using MODTRAN 4 for wide range of atmospheric profiles, spectral emissivity, satellite zenith angle (SZA), and various lapse-rate conditions, including surface inversions. The sensitivity analysis showed that the LST algorithm reproduces LST with a reasonable quality. However, the algorithm has a tendency to overestimate and underestimate for surface inversion and superadiabatic conditions, respectively, especially for warm temperatures, and its performance is superior when the SZA is small and the lapse rate is neutral. Validation results using Moderate-Resolution Imaging Spectroradiometer (MODIS) LST data showed that the correlation coefficients and RMSE are about 0.83~0.99 and 1.09~4.06 K, respectively. The quality of retrieved LST is significantly better at nighttime than at the daytime and in vegetated areas rather than barren areas. The validation results showed that the LST retrieval algorithm developed in this study could be used for operational retrieval of LST from data compiled by MTSAT-1R and Korea’s Communication, Ocean and Meteorological Satellite (COMS), with some modifications.

동성결혼에 따른 현대결혼제도의 위기에 관한 검토

Ki Ok Hong 위기관리 이론과 실천 2014 Crisisonomy Vol.10 No.11

전통적으로 가부장적 대가족제도를 유지하여 왔던 우리나라가 현대이후 대가족해체, 핵가족화, 이혼율 증가, 한 부모 내지 조손가족 증가, OECD국가 중 최저 출산율 등으로 가족제도의 위기를 맞고 있다. 이대로 가면 대한 민국은 2030년 이후 인구가 2천만으로 감소하고 2050년 이후 한민족이 멸망할 것이라는 극단적 전망까지 나오 고 있다. 이에 더불어 양성간의 결혼이 아닌 동성간 결혼문제가 새로운 사회적 현상으로 등장하고, 이에 대한 법 적 허용을 둘러싸고 사회적 여론이 비등하고 있다. 현재 네덜란드, 캐나다, 덴마크 등 20개국 가까운 서구제국에 서 동성간의 혼인을 허용하고 있다. 김조광수, 김승환 커플이 2013년 9월 7일 공개 결혼식을 하고 12월 10일 서 대문구청에 혼인신고서를 제출했으나 13일에 “민법상 당사자간의 혼인의 합의가 없다”라는 이유 등으로 불수리 처분을 받았다. 그래서 금년 5월 21일에 우리나라 최초로 동성혼 소송을 제기하였다. 우리나라 헌법은 제36조 제1항에서 “혼인과 가족생활은 개인의 존엄과 양성의 평등을 기초로 성립되고 유지되어야 하며, 국가는 이를 보 장한다”고 규정하고 있는데, 여기서 “양성의 평등”을 반드시 이성간의 결합만을 의미하는 것은 아니다. 혼인의 개념에 동성간의 결합이 포함되는지 여부에 관한 논란은 결국 헌법에 명시적 규정을 두거나, 혼인대체제도로 개 별법이 도입되기 전까지는 동성간의 혼인이 허용될 수 있는지 여부는 헌법해석의 문제로 남게 된다. 이 논문에서 는 현대 가족제도 및 결혼제도의 위기현상을 진단하고, 동성간의 결혼문제를 법적으로 어떻게 대응하고 해결해야 하는가를 고민하면서 다양한 견해와 해결책을 모색하고자 한다. Our country, one that sustained a patriarchal extended family system throughout history, has come face-to-face with a threat to its family system stemming from the disintegration of extended families, nuclearization of families, increasing divorce dates, growing number of single parent or grandparent-headed households, and the lowest birth rate among OECD nations in the modern era. If current trends hold, the Korean population is expected to dwindle down to 20 million by 2030. Some radical studies go as far as predicting that Koreans will go extinct by 2050. In addition, homosexual marriage, not heterosexual marriage, has newly emerged as a social phenomenon, and the media is boiling over with the issue of legalizing it. The homosexual marriage is permitted in almost twenty countries such as the Netherlands, Canada, Denmark and so on. The couples of Kim Jo Gwang-su and Kim Seung-hwan got married on September 7, 2013 and submitted the marriage declaration. However, it was not accepted due to the reason that there is no civil marriage agreement between the parties. Hence, they filed for the homosexual marriage for the first time in the nation on May 21 this year. It is implied that the equality of gender in the marriage concept of Article 36 Provision 1 of our constitution isn t necessarily the marriage between a man and a woman. With regard to the dispute on whether the homosexual marriage is included in the marriage concept, the permission on the homosexual marriage is left as an issue of the constitutional interpretation until a specific regulation is included in the Constitution of the Republic of Korea or a separate law is introduced as the marriage replacement system.