<i>In vitro</i> and <i>in vivo</i> evaluation of a novel polymer-free everolimus-eluting stent by nitrogen-doped titanium dioxide film deposition

Park, Dae Sung,Bae, In-Ho,Jeong, Myung Ho,Lim, Kyung Seob,Sim, Doo Sun,Hong, Young Joon,Lee, So-Youn,Jang, Eun Jae,Shim, Jae-Won,Park, Jun-Kyu,Lim, Han Chul,Kim, Han Byul Elsevier 2018 Materials Science and Engineering C Vol.91 No.-

<P><B>Abstract</B></P> <P>Inflammation and thrombosis are linked to the use of polymer-based drug-eluting stents (DES). The aim of this study was to develop a polymer-free everolimus (EVL)-eluting stent using nitrogen-doped titanium dioxide (N-TiO<SUB>2</SUB>) and verify its efficacy by <I>in vitro</I> and <I>in vivo</I> assessment in a porcine coronary model. Various analytical approaches such as scanning electron microscopy and atomic force microscopy, electron spectroscopy, Fourier transform infrared spectrometry and contact angle measurement were employed for the characterization. As a part of biocompatibility assessment, platelet adhesion and smooth muscle cell (SMC) proliferation were examined. Bare metal stent (BMS), N-TiO<SUB>2</SUB> stent, everolimus-eluting N-TiO<SUB>2</SUB> (N-TiO<SUB>2</SUB>-EVL) stent, and commercialized EVL-eluting stent (EES) were randomly placed in forty coronary arteries in twenty pigs. After four weeks of implantation, the stents were subjected to histological and quantitative analysis. The N-TiO<SUB>2</SUB> film used in this study was well coated without any cracks or peeling. Surface hydrophilicity (88.8% of angle decrement) could be associated with the decrease in surface roughness post N-TiO<SUB>2</SUB> deposition (37.0%). The platelet adhesion on the N-TiO<SUB>2</SUB> surfaces was less than that on the BMS surface. The proliferation of SMC was suppressed in the N-TiO<SUB>2</SUB>-EVL group (30.2%) but not in the BMS group. In the animal study, the percent area restenosis was significantly decreased in the N-TiO<SUB>2</SUB>-EVL group compared to that in the BMS group. The results (BMS; 47.0 ± 11.00%, N-TiO<SUB>2</SUB>-EVL; 31.7 ± 10.50%, and EES; 29.1 ± 11.21%, <I>n</I> = 10, <I>p</I> < 0.05) were almost at par with those of the commercialized EVL-eluting stent. The introduction of N-TiO<SUB>2</SUB> deposition during fabrication of polymer-free DES may be an efficient accessorial process for preventing in-stent restenosis and thrombosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> N-TiO<SUB>2</SUB> surfaces can help to reduce the platelet adhesion. </LI> <LI> In porcine model, N-TiO<SUB>2</SUB> everolimus decreased in-stent restenosis and fibrin deposition. </LI> </UL> </P>

토끼 赤血球 및 血漿 Cholinesterase 活性에 미치는 影響

金漢燮,金相仁 中央醫學社 1967 中央醫學 Vol.12 No.1

The in vivo effects of anticholinesterases in general vary not only on species, sex, means of administration, vehicle with which an anticholinesterase is administered, etc., but among others on the quality e. g. stability and purity of the preparations. There has been no report, experimental or clinical, concerning the influence of those anticholinesterase pesticides, sold and used in Korea, on the blood cholinesterase in mammals. The erythrocyte and plasma cholinesterase activities were traced in 20 grouped rabbits 6,24 and 72 hours after a single oral administration of 3 organophosphate anticholinesterases-Parathion, EPN and Malathion, and one carbamate anticholinesteraseDithane, -all korean products-in doses of one-third of estimated LD50's for rabbits. 1) Following doses of these compounds an inhibition of cholinesterase was noted in both erythrocyte and plasma, especially at 6 hours. 2) Plasma cholinesterase could be more depressed than erythrocyte enzyme (in cases of Parathion and Dithane groups) when the enzyme depression was marked, i.e. at 6 hours post-administration. 3) At 24 hours there was a tendency that the cholinesterase activities in erythrocyte and plasma dissociate from each other with the plasma enzyme mostly recovered, which the erythrocyte enzyme did not. 4) By 72 hours the erythrocyte cholinesterase was belatedly but mostly recovered. 5) Marked individual variation in cholinesterase activity was noted in each expert mental group. 6) Measurements of cholinesterase activities in 12 normal adult Korean males and 12 females were tried using Michel’s method, with the results (expressed as ?pH/hr): males: erythrocyte, 0.765± 0.121 (S. D. =Standard Deviation), plasma, 0.685 ±0.142 (S.D.); females: erythrocyte, 0.732 ±0.059 (S.D.), plasma, 0.639±0.257 (S.D.).

가막만에 출현하는 동물플랑크톤의 종조성과 계절별 출현 양상

서호영,이인태,윤양호,최상덕,이삼노,한명일,김병섭,강윤호,이우범 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.2

가막만에 출현하는 동물플랑크톤을 계절별(2001년 4월, 7월, 9월, 12월)로 Norpac네트를 이용하여 수직 채집하였다. 우점적으로 출현한 분류군은 4월과 12월에 요각류, 7월에 요각류와 지각류,9월에 야광충이었다. 출현 개체수는 22~17,197indiv. m^-3으로 시·공간적으로 변동폭이 매우 컸다. 요각류의 우점종은 4월에 Euryte-mora pacifica, Acartio omorii, Cenfropages abdominalis, Colanus sinicus, 7월에 Lobidocera rofunda와 A. erythraea, 9월에 A. eryfhraea와 Paracalanus parvus s. l., 12월에 A. omorii와 E. pacifca로 주로 내만종으로 변하였다. 그러나, 9월에 외양종인 Eucalanus sp.와 Neo-calnus sp. 또한 다수 출현하였다. 이와 같은 출현 경향은 가막만이 폐쇄성 내만임에도 불구하고 동물플랑크톤의 분포 특성은 내만수 이외에 계절적으로 외만수의 영향을 강하게 받고 있음을 암시하고 있다. Zooplankton was sampled vertically with a Norpac net from Gamag Bay in April, July, September and December, 2001. Copepods were predominant in April and December, and cladocerans in July and Noctiluca scintillans in September, respectively. There are high spatio-temporal fluctuations in the abundance of zooplankton with a range of 22∼17,197 indiv./㎥. In the copepod community, neritic species, Eurytemora pacifica, Acartia omorii, Centropages abdominalis and Calanus sinicus were predominant in April; Labidocera rotunda and A. erythraea in July; A. erythraea and Paracalanus parvus s. l. in September; A. omorii and E. pacifica in December. However, oceanic species Eucalanus sp. and Neocalanus sp. were abundant in September. It indicates that although Gamag Bay is semi-closed, the distribution pattern of zooplankton is seasonally strongly affected by oceanic waters in addition to neritic ones.

Evaluation of ion implantation for anti-thrombogenic coronary stent in vitro and in vivo

Shim, Jae-Won,Bae, In-Ho,Park, Dae Sung,Lim, Kyung-Seob,Lee, So-Youn,Jang, Eun-Jae,Park, Jun-Kyu,Kim, Ju Han,Jeong, Myung Ho Elsevier 2017 Journal of industrial and engineering chemistry Vol.54 No.-

<P><B>Abstract</B></P> <P>The aim of this study was to evaluate the effect of ion implantation on the surface of anti-thrombogenic coronary stent. Nitrogen (N) was implanted into the cobalt–chromium surface by using an ion source under a plasma environment to enhance its hardness and surface modification. The N ion dosage was 1×10<SUP>15</SUP> ions/cm<SUP>2</SUP>. All analysis results of the ion implanted stent were compared with those of bare metal stent. The N component and distribution were confirmed by auger electron spectroscopy. Microhardness was significantly increased after 40min of implantation (415.3±12.38 HV, 18.9±2.62%). The surface was altered to hydrophobic status through ion implantation (30.6±1.12% in contact angle increment). Platelet adhesion, and smooth muscle cell migration and proliferation were prevented in the ion implanted group (43.9%, 11.2%, and 45.1%, respectively). To verify the in vitro result, stents were implanted to rabbit iliac artery and isolated at 4 weeks post implantation. Then, the stents were subjected to histological analysis. No significant differences in injury score, internal elastic lamina, lumen area, and restenosis rate were found. However, the fibrin score was more significantly decreased in the ion beam-implanted group (2.5±0.15) than in the bare metal stent group (1.8±0.31, <I>n</I> =10, <I>p</I> <I><</I> 0.05). Taken together, ion beam implantation may be an efficient accessorial tool for preventing in-stent restenosis and in-stent thrombosis.</P>

Multicenter Prospective Study of Laparoscopic Nissen Fundoplication for Gastroesophageal Reflux Disease in Korea

Sungsoo Park,Joong-Min Park,Jin-Jo Kim,In-Seob Lee,Sang-Uk Han,Kyung Won Seo,Jin Won Kwon 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.3

Backgrounds/AimsThis multicenter study aims to evaluate the effect and feasibility of anti-reflux surgery compared with medical treatment forgastroesophageal reflux disease (GERD). MethodsPatients with GERD who were undergoing medical treatment with proton pump inhibitors for more than 8 weeks and those whowere scheduled to undergo anti-reflux surgery were enrolled. Efficacy of pre-operative medical treatment was evaluated retrospectivelyand effect of anti-reflux surgery was prospectively evaluated at 1 week and 3 months after surgery. Quality of life (QOL) was alsoinvestigated before and after surgery. ResultsBetween February and October 2018, 51 patients underwent laparoscopic Nissen fundoplication for treating GERD at 5 hospitalsin Korea. Thirty-four patients (66.7%) showed poor proton pump inhibitor response. At 3 months after surgery, heartburn wascompletely resolved in 87.9% patients and partially improved in 9.1%. Acid regurgitation was completely resolved in 82.9% andpartially improved in 11.4%. Atypical extraesophageal symptoms were completely controlled in 45.5% and partially controlled in36.4%. GERD-related QOL scores at 1 week after surgery significantly improved compared with pre-operative scores. There was nodifference in GERD-related QOL scores between 1 week and 3 months after surgery. General QOL measured with European QOL-5dimensions and health-related QOL instrument with 8 items significantly improved after anti-reflux surgery. Satisfaction with treatmentwas significantly higher after surgery than before surgery (72.5% vs 11.8%, P < 0.001). ConclusionAnti-reflux surgery improved GERD symptoms and QOL in patients. Anti-reflux surgery is an effective treatment option compared withmedical treatment for GERD patients selected for surgical treatment.

여성 요실금 환자의 불안과 우울 : 체외자기장신경치료 후의 호전 Comparison of before and after Treatment of Extracoporeal Magnetic Innervation:ExMI

오강섭,왕인경,한종설 한국정신신체의학회 2003 정신신체의학 Vol.11 No.1

Identification of genetic polymorphisms in Korean rice accessions by Whole-genome sequencing

In-Seon Jeong,Ung-Han Yoon,Gang-Seob Lee,Hyeon-So Ji,Hyun-Ju Lee,Tae-Ho Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Advances in genome sequencing technologies have aided discovery of millions of genome-wide DNA polymorphisms, single nucleotide polymorphisms (SNPs) and insertion-deletion (InDels), which are an invaluable resource to analyze genetic diversity in a population. We performed whole-genome resequencing of ten Korean rice accessions including six cultivars and four mutant lines. A total of 2,447 million raw reads were generated with over 58x coverage and detected 3,240,025 DNA polymorphisms between the Korean rice accessions and Nipponbare as reference genome. We observed that in ten Korean rice accessions, the frequency of potential SNPs was estimated to be one per 2.1kb on Nipponbare (382Mb). Potential SNPs were classified into two types, homozygous SNP and heterozygous SNP, which approximately 87% of the total was homozygous SNPs from ten accessions and heterozygous SNPs accounted for 13%. According to annotation of DNA polymorphisms, 634,620 SNPs were found in gene region, and only 169,738 SNPs were occurred in coding region. Altogether, 86,251 non-synonymous SNPs were located on 76,891 genes. We also examined genes which had at least one SNP in all ten accessions. It was estimated that the total of 290 genes had one or more non-synonymous SNPs and 25 genes had only synonymous SNPs. These genes were functionally classified based on gene ontology (GO). These DNA polymorphisms obtained from our result will provide an invaluable resource to identify molecular markers and genes associated with diverse traits of agronomical importance.

Identification of genetic diversity and DNA polymorphisms in Korean rice accessions through resequencing

In-Seon Jeong,Ung-Han Yoon,Gang-Seob Lee,Hyeon-So Ji,Hyun-Ju Lee,Tae-Ho Kim 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

With the rapid development of sequencing technologies, next-generation sequencing is widely utilized for molecular breeding in several crops including rice. We performed whole genome resequencing of ten Korean rice accessions including six cultivars and four mutant lines. In total, 2,448 million raw reads were generated with over 58x coverage of Nipponbare genome. We mapped the reads from each of the ten accessions onto genomic sequence of japonica rice cultivar, Nipponbare. We detected 3,144,016 SNPs, which estimated to be one per 2.2kb on average. We found SNPs in genes that have been reported to be involved in rice flowering time regulation and bacterial blight resistance among ten rice accessions. Unmapped region against Nipponbare genome occupied about 1 ~ 2% in each accession. Over 50% of the unmapped region were found in the repeat region. The minimum length of gap in all accessions were 1bp and the maximum length of gap was 45,967bp in Ilpum. We also identified 3,497 possible gene loss events within these unmapped regions. The frequency of gene loss in each chromosome ranged from 33 on chromosome 5 to 913 on chromosome 11. The genetic variations we detected among ten rice accessions will provide invaluable resources for identification of genes associated with diverse traits of agronomical importance for molecular breeding.

Identification of candidate genomic region associated with seed longevity by re-sequencing in rice

In-Seon Jeong,Jun-Hyeon Cho,Chang-Deok Han,Hyun-Ju Lee,Hyeon-So Ji,Gang-Seob Lee,Ung-Han Yoon,Jang-Ho Hahn,Tae-Ho Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

It is well known that Dharial (Bangladesh origin and weedy rice line) has longer seed longevity than indica and japonica rice varieties. To study the genetic basis of seed longevity of Dharial, we developed 240 BC3F7 backcross recombinant inbred lines derived from the crosses between Dharial (a donor parent) and two korea rice accessions (recurrent parents) including Ilmi and Gopum, respectively. Among these lines, we selected two introgression lines with longer seed longevity and named them Ilmi-NIL and Gopum-NIL. Also, we developed an EMS-induced mutant line from Dharial which has shortened seed longevity, and named it Dharial-EMS. We performed re-sequencing of four rice accessions that are Dharial, Dharial-EMS, Ilmi-NIL, and Gopum-NIL. A total of 706×106 raw reads were generated which provided sequence data over 46x rice genome coverage per each accession. We did genome-wide variation analysis comparing produced re-sequencing data and the re-sequencing data of Ilmi from NABIC database with the Nipponbare reference sequence. By graphical analysis of SNP distribution in rice genome of the five accessions, we could select candidate chromosomal segments introgressed from Dharial in Ilmi-NIL and Gopum-NIL. The introgressed chromosomal segments were in seven regions in Ilmi-NIL and eight regions in Gopum-NIL, and four common introgressed regions between Ilmi-NIL and Gopum-NIL were identified. 2,758 SNPs between Dharial and Dharial-EMS were found in the introgressed regions. Also, we detected 450 genes including at least one SNP among these SNPs. This result will facilitate identification of genes and development of molecular markers for improvement of seed longevity.

Variations in the number of CCL3L1 gene copies and Kawasaki disease in Korean children.

Kim, Hye-Eun,Kim, Jae-Jung,Han, Myung Ki,Lee, Kyung-Yil,Song, Min Seob,Lee, Hyoung-Doo,Kim, Dong Soo,Yu, Jeong Jin,Park, In-Sook,Yun, Sin Weon,Hong, Young Mi,Jang, Gi Young,Lee, Jong-Keuk Springer International] 2012 Pediatric cardiology Vol.33 No.8

<P>High-dose intravenous immunoglobulin (IVIG) therapy is the highly effective and standard treatment for Kawasaki disease (KD). However, similar to 20 % of KD patients have persistent fever or recurrence of fever after the initial IVIG treatment, which increases the risk for coronary artery lesions (CALs). Furthermore, the mechanism of IVIG resistance in KD patients still is unknown. The number of CC chemokine ligand 3-like 1 (CCL3L1) gene copies is reported to be associated with KD and IVIG resistance in Japanese patients. In addition, the authors observed significant upregulation of the CCL3L1 gene expression after in vitro immunoglobulin treatment in B cell lines derived from KD patients. Therefore, this study of 459 KD patients and 496 healthy control subjects tested whether the number of CCL3L1 gene copies is associated with a risk of KD, CALs, and/or IVIG resistance in Korean KD patients. However, the number of CCL3L1 gene copies was not associated with KD (P = 0.18), CAL formation (P = 0.062), or the IVIG resistance (P = 0.90). Therefore, the results indicate that the number of CCL3L1 gene copies does not have a role in susceptibility to KD or CALs nor with IVIG resistance in Korean KD patients.</P>