Anti-thrombotic activity of phenolic acids obtained from Salvia miltiorrhiza f. alba in TNF-a-stimulated endothelial cells via the NF-kB/JNK/p38 MAPK signaling pathway

Xianjing Zheng,Haimei Liu,Maoqiang Ma,Jianbo Ji,Faliang Zhu,Longru Sun 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4

Over the past 100 years, Salvia miltiorrhiza f. alba (Lamiaceae) (RSMA) roots have been used to curethromboangiitis obliterans (TAO) in local clinics. This studyaimed to confi rm the anti-thrombotic effi cacy of 12 phenolicacids obtained from RSMA and to clarify the possibleunderlying mechanisms. The results of quantitative real-timepolymerase chain reaction (qRT-PCR) and enzyme-linkedimmunosorbent assay (ELISA) experiments demonstratedthat most of the phenolic acids markedly inhibited PAI-1protein and mRNA levels but increased t-PA protein andmRNA levels in TNF-α-induced EA.hy926 cells ( P < 0.05or 0.001), with lithospermic acid displaying the strongesteff ect. In vitro anticoagulation and antiplatelet aggregationassays showed that lithospermic acid and salvianolic acidB signifi cantly prolonged prothrombin time (PT), activatedpartial thromboplastin time (APTT), decreased fi brinogen concentration (FIB), and inhibited platelet aggregationinduced by adenosine diphosphate (ADP) in rat blood. Bothlithospermic acid and salvianolic acid B markedly down-regulatedthe expression of factor Xa and factor IIa on the externalsurface of EA.hy926 cells and demonstrated signifi cantanti-factor IIa and anti-factor Xa activity using chromogenicsubstrates in vitro. Western blot results revealed that bothlithospermic acid and salvianolic acid B also signifi cantlyinhibited the expression of TF, p-p65, p-p38, and pJNK proteinsinduced by TNF-α. These results indicated that all ofthe phenolic acids appeared to have some anti-thromboticactivity, with salvianolic acid B and lithospermic acid markedlydecreasing the chance of thrombosis by regulating theNF-κB/JNK/p38 MAPK signaling pathway in response toTNF-α.

Synergistic enhancement of external electrical energy in PM filtration with Cu-HHTP@CuO nanowire arrays coated copper mesh

Haiyan Li,Haimei Liu,Cheng Qian,Tao Wang,Sheng Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

Developing multifunctional purification systems that combine air filtration and antibacterial functionalityis a challenging task. However, in designing air filtration materials, achieving a balance between filtrationefficiency and pressure drop remains a significant challenge. In this study, we developed anovel filtration material composed of hierarchically 3D Cu-HHTP nanowiskers coated CuO nanowires thatwere grown in-situ on a copper mesh. Benefitted from the intrinsically electrical conductivity of Cu-HHTP, we combined the composite material with external electrical energy for air filtration. By applyinglower electric potential directly (voltage of 5 V), the 3D conductive mesh filter exhibited excellent performancesin terms of the PM2.5 removal efficiency (99.7%), pressure drop (32 Pa at 2 cm s -1), andquality factor (0.187 at 2 cm s -1). Furthermore, after ten cycles of filtration and cleaning, the materialmaintained a high PM removal rate. In addition to its excellent filtration performance, the compositemetal mesh also exhibited outstanding antibacterial activity against both E. coli and S. aureus. Theelectro-mechanical stability and recyclability of the composite filter make it a cost-effective and environmentallyfriendly solution for air purification, with promising potential for improving air quality.

Polymorphism of Estrogen Receptor Genes and Its Interactions With Neurodevelopmental Genes in Attention Deficit Hyperactivity Disorder Among Chinese Han Descent

Yiwei Lin,Haimei Li,Jing Zhang,Ziqi Yang,Yi Zhou,Lu Liu,Qiujin Qian 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.8

Objective Attention deficit hyperactivity disorder (ADHD) is a polygenic neurodevelopmental disorder with significant gender differences. The sexual dimorphism of ADHD may be associated with estrogen acting through estrogen receptors (ESR). This study investigates the impact of <i>ESR</i> gene polymorphism and its interactions with neurodevelopmental genes on ADHD susceptibility.Methods The study compared genotyping data of single nucleotide polymorphisms in <i>ESR1</i> and <i>ESR2</i> in 1,035 ADHD cases and 962 controls. The gene-gene interactions between <i>ESR</i> genes and three neurodevelopmental genes (brain-derived neurotrophic factor [<i>BDNF</i>], synaptosomal-associated protein of 25 kDa gene [<i>SNAP25</i>], and cadherin-13 [<i>CDH13<i></i></i>]) in ADHD were investigated using generalized multifactor dimensionality reduction and verified by logistic regression analysis.Results The G allele of rs960070/<i>ESR2</i> (empirical p=0.0076) and the A allele of rs8017441/<i>ESR2</i> (empirical p=0.0426) were found significantly higher in ADHD cases than in the controls but not in male or female subgroups. Though no difference was found in all subjects or females, the A allele of rs9340817/<i>ESR1</i> (empirical p=0.0344) was found significantly higher in ADHD cases than controls in males. We also found genetic interaction models between <i>ESR2</i> gene, neurodevelopmental genes and ADHD susceptibility in males (<i>ESR2</i> rs960070/<i>BDNF</i> rs6265/<i>BDNF</i> rs2049046/<i>SNAP25</i> rs362987/<i>CDH13</i> rs6565113) and females (<i>ESR2<i></i></i> rs960070/<i>BDNF<i></i></i> rs6265/<i>BDNF</i> rs2049046) separately, though it was negative in overall subjects.Conclusion The <i>ESR</i> gene polymorphism associates with ADHD among Chinese Han children, with interactions between <i>ESR<i></i></i> genes and neurodevelopmental genes potentially influencing the susceptibility of ADHD.

The Clinical Manifestation, Executive Dysfunction, and Caregiver Strain in Subthreshold Attention-Deficit/Hyperactivity Disorder

Yajing Tang,Sunwei Qiu,Haimei Li,Feifei Si,Mengjie Zhao,Min Dong,Meirong Pan,Xinxin Yue,Lu Liu,Qiujin Qian,Yufeng Wang 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.9

Objective Subthreshold attention-deficit/hyperactivity disorder (ADHD) has been suggested to be a “morbid condition” which also needs medical attention.Methods The present study recruited 89 children with subthreshold ADHD (sADHD), 115 children with diagnosed ADHD (cADHD), and 79 healthy controls (HC) to explore the clinical manifestation, executive functions (EFs) of sADHD, and the caregiver strain. The clinical manifestation was evaluated through clinical interviews and parent-reports. Executive functions were assessed both experimentally and ecologically. Caregiver strain was measured by a parent-reported questionnaire.Results For the clinical manifestation, both sADHD and cADHD indicated impairments when compared with HC. The comorbidities and the scaled symptoms indicated that the externalizing behaviors were relatively less serious in sADHD than cADHD, whereas the internalizing behaviors between two groups were comparable. For ecological EFs, sADHD scored between cADHD and HC in inhibition and working memory. For experimental EFs, sADHD was comparable to cADHD in inhibition, shifting, and was worse than cADHD in verbal working memory. For the caregiver strain, all scores of sADHD were between that in cADHD and that in HC.Conclusion Our present findings supported the suggestion of subthreshold ADHD as “morbid condition,” which should be treated with caution in clinical practice, especially for the internalizing behaviors and some key components of EFs.

Effects of Dietary Flaxseed Lignan Extract on Symptoms of Benign Prostatic Hyperplasia

Wei Zhang,Xiaobing Wang,Yi Liu,Haimei Tian,Brent Flickinger,Mark W. Empie,Sam Z. Sun 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.2

A flaxseed lignan extract containing 33% secoisolariciresinol diglucoside (SDG) was evaluated for its abilityto alleviate lower urinary tract symptoms (LUTS) in 87 subjects with benign prostatic hyperplasia (BPH). A randomized, dou-ble-blind, placebo-controlled clinical trial with repeated measurements was conducted over a 4-month period using treatmentdosages of 0 (placebo), 300, or 600 mg/day SDG. After 4 months of treatment, 78 of the 87 subjects completed the study.For the 0, 300, and 600 mg/day SDG groups, respectively, the International Prostate Symptom Score (IPSS) decreased . 3.67.1.56, . 7.33. 1.18, and . 6.88. 1.43 (mean. SE, P. .100,. .001, and. .001 compared to baseline), the Quality of Lifescore (QOL score) improved by . 0.71. 0.23, . 1.48. 0.24, and . 1.75. 0.25 (mean. SE, P. .163 and .012 comparedto placebo and P. .103,. .001, and. .001 compared to baseline), and the number of subjects whose LUTS grade changedfrom “moderate/severe” to “mild” increased by three, six, and 10 (P. .188, .032, and .012 compared to baseline). Maximumurinary flows insignificantly increased 0.43. 1.57, 1.86. 1.08, and 2.7. 1.93 mL/second (mean. SE, no statistical sig-nificance reached), and postvoiding urine volume decreased insignificantly by . 29.4. 20.46, . 19.2. 16.91, and . 55.62.36.45 mL (mean. SE, no statistical significance reached). Plasma concentrations of secoisolariciresinol (SECO), enterodiol(ED), and enterolactone (EL) were significantly raised after the supplementation. The observed decreases in IPSS and QOLscore were correlated with the concentrations of plasma total lignans, SECO, ED, and EL. In conclusion, dietary flaxseed lig-nan extract appreciably improves LUTS in BPH subjects, and the therapeutic efficacy appeared comparable to that of com-monly used intervention agents of .1A-adrenoceptor blockers and 5.-reductase inhibitors.