Antitumor bioactivity of porphyran extracted from <i>Pyropia yezoensis</i> Chonsoo2 on human cancer cell lines

He, Dan,Wu, Siya,Yan, Liping,Zuo, Jihui,Cheng, Yang,Wang, Hanfei,Liu, Jian,Zhang, Xu,Wu, Mingjiang,Choi, Jong‐,il,Tong, Haibin John WileySons, Ltd 2019 Journal of the Science of Food and Agriculture Vol.99 No.15

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P><I>Pyropia yezoensis</I>, rich in porphyran, is a medicine‐edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high‐yield algal strain <I>Pyropia yezoensis</I> Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated.</P><P><B>RESULTS</B></P><P><I>Pyropia yezoensis</I> porphyran is a water‐soluble, triple‐helical sulfated hetero‐galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP‐20 and PYP‐50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP‐20 and PYP‐50. Three porphyrans had no toxicity in normal human liver cells (HL‐7702) and showed antitumor effects on Hep3B, HeLa and MDA‐MB‐231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5‐fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5‐fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin‐dependent kinase 1.</P><P><B>CONCLUSION</B></P><P><I>Pyropia yezoensis</I> porphyran, as applied to medicine and functional food, could potentially be used as a non‐toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry</P>

Advantages of Restoring miR-205-3p Expression for Better Prognosis of Gastric Cancer via Prevention of Epithelial-mesenchymal Transition

Zhang, Zhen,He, Xujun,Xu, Ji,Zhang, Genhua,Yang, Yue,Ma, Jie,Sun, Yuanshui,Ni, Haibin,Wang, Fengyong The Korean Gastric Cancer Association 2020 Journal of gastric cancer Vol.20 No.2

Purpose: miR-205 is a tumor suppressor and plays an important role in tumor invasiveness. However, the role of miR-205 in human gastric cancer (GC) epithelial-mesenchymal transition (EMT) remains unclear. The aim of this study was to investigate the molecular mechanism of miR-205 in the regulation of EMT in GC invasion. Materials and Methods: Quantitative polymerase chain reaction (qPCR) was used to detect the expression of miR-205 in GC. Further, the correlation between the pathological parameters and prognosis of GC was statistically analyzed. A transwell model was used to evaluate the effect of miR-205-3p on the invasion and migration of GC cells. qPCR, western blotting, and luciferase assay were performed to analyze the relationship and target effects between miR-205-3p and the expression of zinc finger electron box binding homologous box 1 (ZEB1) and 2 (ZEB2). Results: We found that the levels of miR-205-3p were significantly lower (P<0.05) in GC tissues than in matched normal tissues. Additionally, the expression of miR-205-3p was related to the tumor invasion depth, lymph node metastasis, lymph node invasion, and tumor, node, metastasis stage. Patients with lower miR-205-3p expression levels in the tumors had a poorer prognosis. The in vitro assays indicated that miR-205-3p could affect the invasion ability and EMT of GC cells by targeting the expression of both ZEB1 and ZEB2. Conclusions: miR-205-3p promotes GC progression and affects the prognosis of patients by targeting both ZEB1 and ZEB2 to directly influence EMT.

Viscoelastic analysis of a sleeve based on the BP neural network

Yubin Gao,Haibin Li,Guangmei Wei,Yun He 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.11

Viscoelastic materials are widely used in aviation, underground engineering, chemical engineering, light textile, construction, machineryand shock-absorbing. The properties of viscoelastic materials present strong non-linear characteristics in the time, temperature andfrequency domains. The objective is to explore the possibility of having a rational understanding of the complex response. The neuralnetwork technique is thus implemented for this purpose. This study presents the differential constitutive equations of viscoelastic materialsbased on the generalized Kelvin model. The viscoelastic response Laplace transform equations of the sleeve structure are derivedunder the condition of plane strain according to the boundary conditions of the viscoelastic sleeve. The orthogonal design test results ofviscoelastic numerical calculations are normalized and trained using a three-layer BP neural network. The trained network can easilyrealize the mapping of the viscoelastic material test set. Simulation results show that the neural network test and numerical solution resultsare close to each other, and the maximum error of all variables is less than 2.8 percent. The results obtained in this research revealthat the neural network technique has a suitable capability in establishing correlations among different loads and corresponding viscoelasticresponses. The developed neural network model can effectively solve the non-linear problems of viscoelastic structures.

Advantages of Restoring miR-205-3p Expression for Better Prognosis of Gastric Cancer via Prevention of Epithelial-mesenchymal Transition

Zhen Zhang,Xujun He,Ji Xu,Genhua Zhang,Yue Yang,Jie Ma,Yuanshui Sun,Haibin Ni,Fengyong Wang 대한위암학회 2020 Journal of gastric cancer Vol.20 No.2

Purpose: miR-205 is a tumor suppressor and plays an important role in tumor invasiveness. However, the role of miR-205 in human gastric cancer (GC) epithelial-mesenchymal transition (EMT) remains unclear. The aim of this study was to investigate the molecular mechanism of miR-205 in the regulation of EMT in GC invasion. Materials and Methods: Quantitative polymerase chain reaction (qPCR) was used to detect the expression of miR-205 in GC. Further, the correlation between the pathological parameters and prognosis of GC was statistically analyzed. A transwell model was used to evaluate the effect of miR-205-3p on the invasion and migration of GC cells. qPCR, western blotting, and luciferase assay were performed to analyze the relationship and target effects between miR-205-3p and the expression of zinc finger electron box binding homologous box 1 (ZEB1) and 2 (ZEB2). Results: We found that the levels of miR-205-3p were significantly lower (P<0.05) in GC tissues than in matched normal tissues. Additionally, the expression of miR-205-3p was related to the tumor invasion depth, lymph node metastasis, lymph node invasion, and tumor, node, metastasis stage. Patients with lower miR-205-3p expression levels in the tumors had a poorer prognosis. The in vitro assays indicated that miR-205-3p could affect the invasion ability and EMT of GC cells by targeting the expression of both ZEB1 and ZEB2. Conclusions: miR-205-3p promotes GC progression and affects the prognosis of patients by targeting both ZEB1 and ZEB2 to directly influence EMT.

Perovskite-type LaFe1− xMnxO3 (x=0, 0.3, 0.5, 0.7, 1.0) oxygen carriers for chemical-looping steam methane reforming: Oxidation activity and resistance to carbon formation

Kun Zhao,Fang He,Zhen Huang,Guoqiang Wei,Anqing Zheng,Haibin Li,Zengli Zhao 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.6

The effects of Mn substitution of LaMnxFe1−xO3 (x=0, 0.3, 0.5, 0.7, 1.0) on the oxidation activity and resistance to carbon formation for chemical-looping steam methane reforming (CL-SMR) were investigated. The desired crystalline perovskite phases were formed by transferring from the orthorhombic structure of LaFeO3 to rhombohedral lattice of LaMnO3 as the degree of Mn-doping increased. Manganese ions have a mixed state of Mn3+ and Mn4+ in the LaFe1−xMnxO3, meanwhile inducing the states of highly mixed character of Fe2+, Fe3+ and Fe4+ in iron ions. Substitution of Mn for Fe with proper value not only increases the lattice oxygen, which is conducive to the partial oxidation of CH4 to produce syngas, but also enhances the lattice oxygen mobility from the bulk to the surface of the oxygen carrier particles. Judging from the points of the redox reactivity, resistance to carbon formation and hydrogen generation capacity, the optimal range of the degree of Mn substitution is x=0.3-0.5.

1인승 태양광 자동차의 전기 장치 효율과 재원이 주행에 끼치는 영향에 관한 연구

정연수(Yeon-soo Jeong),하양(Yang He),유해빈(Haibin Liu),이승현(Seung-hyun Lee),김철호(Chul-Ho Kim) 한국자동차공학회 2014 한국자동차공학회 학술대회 및 전시회 Vol.2014 No.11

As the environmental pollution becomes global problem, the importance of eco-friendly cars is magnified. Especially, the solar car has high potential caused that many solar car races are being held around the world. In this paper, the various factors of the solar car components are analyzed by using power consumption model to infer that how factors have an effect on the driving in the Australian environment which is the place being held WSC. Based on the analysis, motor and solar cell efficiencies have a most impact on running performance.

OsCAS: a comprehensive web-based annotation platform for rice microarray data

Qingyun Shi,Yijun Meng,Dijun Chen,Fei He,Haibin Gu,Ping Wu,Ming Chen 한국바이오칩학회 2010 BioChip Journal Vol.4 No.1

OsCAS (Oryza sativa Chip Annotation System) is a comprehensive web-based tool that analyzes the results of rice (Oryza sativa) microarray experiments and the potential relationships among genes. This platform is designed to facilitate the indepth exploration of microarray data with relatively high analysis efficiency. With a user-friendly web interface, OsCAS allows chip probe IDs as inputs to retrieve relevant information according to user’s designation. Public databases, such as GenBank, UniGene, Swiss-Prot, TIGR, KOME, KEGG, Gene Ontology, and miRBase, were integrated into our platform to cover gene information, protein features, metabolic pathways and regulatory factors in rice. Besides, OsCAS includes the reprocessed information from several analytical tools such as CSRDB and miRU to obtain deep insights into the primary annotations. OsCAS is time-cost-effective in annotating large sets of chip probe IDs, and can provide some precious hints for the biological experiment design. OsCAS runs on a Linux server and is accessible at http://bis.zju.edu.cn/oscas/.

MiRNA320a Inhibitor-Loaded PLGA-PLL-PEG Nanoparticles Contribute to Bone Regeneration in Trauma-Induced Osteonecrosis Model of the Femoral Head

Zhang Ying,Li Chuan,Wei Qiushi,Yuan Qiang,He Wei,Zhang Ning,Dong Yiping,Jing Zhenhao,Zhang Leilei,Wang Haibin,Cao Xiangyang 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH. BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.

Stability of phenolic acids and the effect on weed control activity

Li, Jiayu,Zhang, Qi,Hu, Wenwen,Yang, Xiaoyan,He, Haibin 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

Phenolic acid is a very important class of allelochemicals with allelopathic weed control activity. In this study, three benzoic acid derivatives (syringic, 4-hydroxybenzoic, and vanillic acids), three cinnamic acid derivatives (cinnamic, 4-hydroxycinnamic, and ferulic acids) were tested, and high-performance liquid chromatography was used to conduct a dynamic analysis on the changes in the concentration of phenolic acids in a bioassay based on the initial concentration and test time. The results showed that the concentration of individual phenolic acids and a solution of mixed phenolic acids decreased to a certain extent irrespective of environment, i.e., bioassay (4-7 days) or a ricegrowing environment, and a significant decrease in concentration was measured after 48 h. Based on the above results, the laboratory bioassay was conducted using a fresh solution of phenolic acids every 48 h. The results showed that the instability of phenolic acid could affect its weed control activity, and this effect was more significant for high concentrations of phenolic acids. On the other hand, changing the solution did not have a significant impact on the weed control activity of phenolic acids in the natural environment (pH 6.50), in which allelopathic rice release phenolic acids. These results reveal the instability of phenolic acids could significantly reduce the inhibition rate on the growth index for receptor plants in an indoor bioassay.

Metabolic Regulation of Gene Expression by Histone Lysine β-Hydroxybutyrylation

Xie, Zhongyu,Zhang, Di,Chung, Dongjun,Tang, Zhanyun,Huang, He,Dai, Lunzhi,Qi, Shankang,Li, Jingya,Colak, Gozde,Chen, Yue,Xia, Chunmei,Peng, Chao,Ruan, Haibin,Kirkey, Matt,Wang, Danli,Jensen, Lindy M. Elsevier 2016 Molecular cell Vol.62 No.2

<P><B>Summary</B></P> <P>Here we report the identification and verification of a β-hydroxybutyrate-derived protein modification, lysine β-hydroxybutyrylation (Kbhb), as a new type of histone mark. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. In total, we identified 44 histone Kbhb sites, a figure comparable to the known number of histone acetylation sites. By ChIP-seq and RNA-seq analysis, we demonstrate that histone Kbhb is a mark enriched in active gene promoters and that the increased H3K9bhb levels that occur during starvation are associated with genes upregulated in starvation-responsive metabolic pathways. Histone β-hydroxybutyrylation thus represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lysine β-hydroxybutyrylation (Kbhb) is a new type of histone mark </LI> <LI> 44 non-redundant histone Kbhb sites are identified in human and mouse cells </LI> <LI> Histone Kbhb increases under starvation and STZ-induced ketoacidosis </LI> <LI> Starvation-induced H3K9bhb is associated with active gene expression </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>