Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

Formaldehyde 처리 귀리의 젖소 소화관내 영양소 분해율 측정

이상철(S . C . Lee),김병기(B . K . Kim),문여황(Y . H . Moon),문점동(C . D . Moon),안병홍(B . H . Ahn) 한국영양사료학회 1996 韓國營養飼料學會誌 Vol.20 No.1

난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

H₂ pressure swing adsorption for high pressure syngas from an integrated gasification combined cycle with a carbon capture process

Moon, D.K.,Lee, D.G.,Lee, C.H. Applied Science Publishers 2016 APPLIED ENERGY Vol.183 No.-

<P>The integrated gasification combined cycle (IGCC) process, possessing high efficiency and environmental advantages, produces H-2-rich syngas at high pressures (30-35 bar) after capturing CO2. Since the syngas pressure is very high for conventional PSA processes, development of an efficient PSA process at the pressure conditions is required for H-2 production. In this study, the H-2 PSA process for IGCC syngas was developed experimentally and theoretically. Breakthrough and PSA experiments using activated carbon or activated carbon/zeolite LiX were performed at 25-35 bar by using a five-component hydrogen mixture (H-2:CO:N-2:CO2:Ar = 88:3:6:2:1 mol%) as a simulated syngas. The overall PSA performance was evaluated in terms of the purity, recovery and productivity of H-2 product. According to the results from using single or layered beds, the two-bed PSA process produced 99.77-99.95% H-2 with 73.30-77.64% recovery experimentally. A four-layered bed PSA at 35 bar was able to produce 99.97%-purity H-2 with 79% recovery, and it contained Ar and N-2 impurities. The quality of tail gas from the PSA process could be used for the gas turbine without losing H-2 and CO. A rigorous mathematical model that included mass, energy, and momentum balances was employed to elucidate the dynamic behaviors and separation performance of the adsorption bed and PSA process. (C) 2016 Elsevier Ltd. All rights reserved.</P>

HWE(Hot Wall Epitaxy)에 의한 ZnIn_2S_4 박막 성장과 광전도 특성

홍광준,이관교,정준우,정경아,방진주,장현규,문종대,김혜숙 조선대학교 기초과학연구소 1999 自然科學硏究 Vol.22 No.1

HWE 방법에 의해 ZnIn_2S_4 박막을 Si(00) 기판 위에 성장시켰다. 증발원과 기판의 온도를 각각 610℃, 450℃로 하여 성장시킨 ZnIn_2S_4 박막의 이중 결정 X-선 요동곡선(DCRC)의 반폭치(FWHM)값이 245 arcsec로 가장 작았다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자농도의 1n n 대 1/T에서 구한 활성화에너지는 0.17eV로 측정되었다. Hall 이동도의 온도 의존성은 30K에서 100K까지는 불순물산란에 기인하고, 100K에서 293K까지는 격자산란에 기인한것으로 고찰되었다. 광전도셀의 특성으로 spectral response, 최대 허용소비전력(MAPD), 광전류와 암전류(pc/dc)의 비 및 응답시간을 측정하였다. S 증기분위기에서 열처리한 광전도 셀의 경우, 감도(??)는 0.99, pc/dc은 1.37x10^7, 그리고 최대 허용소비전력(MAPD)은 336mW, 오름시간(rise time)은 9ms, 내림시간(decay time)은 9.8ms로 가장 좋은 광전도 특성을 얻었다. The ZnIn_2S_4 thin films were grown on the Si(100) wafers by a hot wall epitaxy method(HWE). The source and substrate temperature are 610℃ and 450℃ respectively. The crystalline structure of epilayers was investigated by double crystal X-ray diffraction(DCXD). Hall effect on the sample was measured by the van der Pauw method and studied on the carrier density and mobility dependence on temperature. From Hall data, the mobility was increased in the temperature range 30K to 100K by impurity scattering and decreased in the temperature range 100K to 293K by the lattice scattering. In order to explore the applicability as a photoconductive cell, we measured the sensitivity(??), the ratio of photocurrent to darkcurrent(pc/dc), maximum allowable power dissipation(MAPD), spectral response and response time. The results indicated that the photoconductive characteristic were the best for the samples annealed in S vapor compare with in Zn, In, air and vacuum vapour. Then we obtained the sensitivity of 0.99, the value of pc/dc of 1.37x10^7, the MAPD of 336mW, and the rise and decay time of 9ms and 9.8ms, respectively.

Cordyceptin induces apoptosis through repressing hTERT expression and inducing extranuclear export of hTERT

Jang, K.J.,Kwon, G.S.,Jeong, J.W.,Kim, C.H.,Yoon, H.M.,Kim, G.Y.,Shim, J.H.,Moon, S.K.,Kim, W.J.,Choi, Y.H. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.119 No.3

Cordycepin is an adenosine analog originally extracted from Cordyceps militaris that possesses many pharmacological effects including immune activation and antioxidant and antitumor effects. However, the underlying relationship between apoptosis and telomerase activity in response to cordycepin exposure has not been investigated. In this study, we found that cordycepin-induced apoptosis of human leukemia cells (H937 and THP-1 cells) was associated with inactivation of telomerase and downregulation of human telomerase reverse transcriptase (hTERT) as well as the transcription factors c-Myc and Sp1, which are required for basal transcription from the hTERT gene promoter. Cordycepin also attenuated the activation of phosphoinositide-3-kinase (PI3K)/Akt signaling, thereby reducing phosphorylation and nuclear translocation of hTERT. We further showed that the PI3K inhibitor LY29004 significantly decreased telomerase activity in cordycepin-treated cells and increased cordycepin-induced cell death. These findings demonstrate that cordycepin is cytotoxic to human leukemia cells and suppresses telomerase activity through transcriptional and post-translational suppression of hTERT by inactivating the PI3K/Akt signaling pathway.

Conversion of organic solid waste to hydrogen and methane by two-stage fermentation system with reuse of methane fermenter effluent as diluting water in hydrogen fermentation

Jung, K.W.,Moon, C.,Cho, S.K.,Kim, S.H.,Shin, H.S.,Kim, D.H. Elsevier Applied Science 2013 Bioresource technology Vol.139 No.-

In this study, a two-stage system converting organic solid waste (food waste+sewage sludge) to H<SUB>2</SUB> and CH<SUB>4</SUB> was operated. In the first stage of dark fermentative hydrogen production (DFHP), a recently proposed method that does not require external inoculum, was applied. In the second stage, anaerobic sequencing batch reactor (ASBR) and an up-flow anaerobic sludge blanket reactor (UASBr) were followed to treat H<SUB>2</SUB> fermenter effluent. (H<SUB>2</SUB>+CH<SUB>4</SUB>-ASBR) system showed better performance in terms of total biogas conversion (78.6%), while higher biogas production rate (2.03L H<SUB>2</SUB>/L<SUB>system</SUB>/d, 1.96L CH<SUB>4</SUB>/L<SUB>system</SUB>/d) was achieved in (H<SUB>2</SUB>+CH<SUB>4</SUB>-UASBr) system. To reduce the alkali addition requirement in DFHP process, CH<SUB>4</SUB> fermenter effluent was tested as a diluting water. Both the ASBR and UASBr effluent was effective to keep the pH above 6 without CH<SUB>4</SUB> production. In case of using ASBR effluent, H<SUB>2</SUB> production dropped by 15%, but alkali addition requirement was reduced by 50%.

CBD 방법에 의한 CdS 박막의 성장과 광전도 특성

황광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdS 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 550℃로 열처리한 시료의 경우 X-선 회절무늬로 부터 외삽법에 의해 a_。와 c_。는 각각 4.1364 Å과 6.7129 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.35㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 150 K 까지는 압전산란에 의하여, 150 K 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdS thin films were grown on creamic substrate using a chemical bath deposition method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdS polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdS samples annealed in N_2 gas at 550℃ it was found hexagonal structure whose lattice constants a_o and c_o were 4.1364 Å and 6.7120Å, respectively. Its grain size was about 0.35 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33K and 150K and by polar optical scattering at temperature range of 150K and 293K. We measured also spectral response, sensitivity (γ), maximum allowable power dissipation and response time on these samples.

Synthesis and characterization of ampelopsin glucosides using dextransucrase from Leuconostoc mesenteroides B-1299CB4: Glucosylation enhancing physicochemical properties

Woo, H.J.,Kang, H.K.,Nguyen, T.T.H.,Kim, G.E.,Kim, Y.M.,Park, J.S.,Kim, D.,Cha, J.,Moon, Y.H.,Nam, S.H.,Xia, Y.m.,Kimura, A.,Kim, D. IPC Science and Technology Press ; Elsevier Scienc 2012 Enzyme and microbial technology Vol.51 No.6

Novel ampelopsin glucosides (AMPLS-Gs) were enzymatically synthesized and purified using a Sephadex LH-20 column. Each structure of the purified AMPLS-Gs was determined by nuclear magnetic resonance, and the ionic product of AMPLS-G1 was observed at m/z 505 (C<SUB>21</SUB>H<SUB>22</SUB>O<SUB>13</SUB>.Na)<SUP>+</SUP> using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. AMPLS-G1 was identified as ampelopsin-4'-O-α-d-glucopyranoside. The optimum condition for AMPLS-G1, determined using response surface methodology, was 70mM ampelopsin, 150mM sucrose, and 1U/mL dextransucrase, which resulted in an AMPLS-G1 yield of 34g/L. The purified AMPLS-G1 displayed 89-fold increased water solubility and 14.5-fold browning resistance compared to those of AMPLS and competitive inhibition against tyrosinase with a K<SUB>i</SUB> value of 40.16μM. This value was smaller than that of AMPLS (K<SUB>i</SUB>=62.56μM) and much smaller than that of β-arbutin (K<SUB>i</SUB>=514.84μM), a commercial active ingredient of whitening cosmetics. These results indicate the potential of AMPLS and AMPLS-G1 as superior ingredients for functional cosmetics.

L-ascorbic acid와 α-tocophero1이 돼지정액 성상과 체외수정란의 배발달율에 미치는 영향

박희진,김은국,위갑인,강만종,김광현,김재홍,문승주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

The objective of this studys were to investigate the effect of α-tocopherol and L-ascorbic acid supplement on the boar semen characteristics and in vitro development rate of porcine oocytes. Sperm viability rate was significantly higher in α-tocopherol supplement than in control(66.6%) after 72hr of storage(P<0.05). Sperm motility was increased in treatment(80%, 93.3%, 85%) than in control(73.3%) at 12h of storage(P<0.05). Following insemination with 60h of store, morular and blastocyst on in vitro development resulted in significantly higher than control(P<0.05). After 72h of semen storage L-ascorbic addition also increased the sperm viability rate(P<0.05). Treatment as sperm motility was significantly higher than control in 72h of store(60% vs 66.6%, p<0.05). Following insemination with 60h of store treated porcine oocytes on in vitro development was increased more than control(56.8%), 59.9%, 62.8% vs 45.1%, P<0.05). Morular and blastocyst on in vitro development resulted in significantly higher than control(27.1%, 30.0%, 33.1% vs 20.0%, P<0.05). The results from this experiment indicated that α-tocopherol and L-ascorbic acid supplementation, for viability and motility in porcine semen, increased delay storing time of semen and in vitro development rate following insemination.