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Jing Li,Huige Song,Nan Dong,Guohua Zhao 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1
Storage (4oC and 25oC, 28 days) and thermal(70oC-90oC, 6 h) stabilities of purple sweet potatoanthocyanins (PSPAs) with varying concentrations ofascorbic acid (AA) were investigated in a model soft drinkmedium. For storage stability, the model drink wassterilized at 85oC for 15 min prior to storage. Zero-orderkinetics and first-order kinetics were fitted for storagedegradation at 4oC and 25oC, respectively. However, alldata for thermal degradation fitted first-order kinetics. Thetemperature dependence on degradation was modeled afterthe Arrhenius equation. Storage degradation of PSPAs wasincreased by the presence of AA (40-360 mg/L). Retardedthermal degradation was be achieved by adding 120 mg/Lof AA, while accelerated thermal degradation resultedfrom 360 mg/L of AA. Heat treatment did not markedlychange the DPPH radical-scavenging activity of PSPAs.
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( Jing Liu ),( Zhen Li ),( Guohua Yu ),( Ting Wang ),( Guimei Qu ),( Yunhui Wang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.10
To clarify the role of long intergenic nonprotein-coding RNA 1232 (LINC01232) in the progression of gastric cancer and the potential mechanism, we analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool, and the LINC01232 level in gastric cancer cell lines was detected by quantitative real time-polymerase chain reaction (qRT-PCR) as well. Cell proliferation assay, colony formation assay, transwell assay and tumor formation experiment in nude mice were conducted to observe the biological behavior changes of gastric cancer cells through the influence of LINC01232 knockdown. LncATLAS database and subcellular isolation assay were used for subcellular distribution of LINC01232 in gastric cancer cells. The interaction among LINC01232, zeste homolog 2 (EZH2) and kruppel-like factor 2 (KLF2) was clarified by RNA-protein interaction prediction (RPISeq), RNA immunoprecipitation (RIP), qRT-PCR and chromatin immunoprecipitation (ChIP) assay. Rescue experiments were further conducted to elucidate the biological function of LINC01232/KLF2 axis in the progression of gastric cancer. LINC01232 was upregulated in stomach adenocarcinoma (STAD) tissues and gastric cancer lines. LINC01232 knockdown inhibited the proliferative capacities of gastric cancer cells in vitro, and impaired in vivo tumorigenicity. LINC01232 was mainly distributed in the cell nucleus where it epigenetically repressed KLF2 expression via binding to the enhancer of EZH2, which was capable of binding to promoter regions of KLF2 to induce histone H3 lysine 27 trimethylation (H3K27me3). LINC01232 exerts oncogenic activities in gastric cancer via inhibition of KLF2, and therefore, the knockdown of KLF2 could reverse the regulatory effect of LINC01232 in the proliferative ability of gastric cancer cells.
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Xiaofei Gao,Guohua Jiang,Liming Ruan,Yanfang Sun,Khaydar E. Yunusov,Yanting Jing,Uladzislau E. Aharodnikau,Sergey O. Solomevich 한국섬유공학회 2023 Fibers and polymers Vol.24 No.2
Electrospun nanofibers represent a novel class of scaffold materials that show great potential in wound healing owing torelatively large surface area, better mimicry of native extracellular matrix, adjustable waterproofness and breathability, andprogrammable drug delivery process. In this work, electrospun polycaprolactone (PCL) and porcine acellular dermal matrix(PADM) composite nanofibrous membranes have been developed by electrospinning technology and inflated into threedimensional(3D) structural scaffold (PCL-PADM) by gas foaming method. The obtained PCL-PADM has higher thermalstability, hydrophilicity and better cell adhesion compared with PCL. In addition, ε-polylysine (ε-PL) is further attachedonto the surface of PADM/PCL to offer its good antibacterial properties. Moreover, the PADM/PCL fibrous scaffolds showexcellent cytocompatibility for promoting cell proliferation. In vivo models showed that the resultant PADM/PCL fibrousscaffolds exhibit an accelerating wound healing effect through promoting expression of vascular factor (CD31) and decreasingthe expression of tumor necrosis factor-α (TNF-α). These results indicate that the 3D fibrous scaffolds may be a potentialwound dressing for wound closure.
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Tianming Lin,Zuoming Zhou,Yixuan Liu,Xiaoyan Wang,Guohua Jing 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
A two-stage bioreduction system containingmagnetic-microsphere-immobilized denitrifying bacteria andiron-reducing bacteria was developed for the regenerationof scrubbing solutions for NOx removal. In this process, ahigher bioreduction rate and a better tolerance of inhibitionof bacteria were achieved with immobilized bacteria thanwith free bacteria. This work focused on evaluation of theeffects of the main components in the scrubbing solutionon Fe(III)EDTA (EDTA: ethylenediaminetetraacetate) andFe(II)EDTA-NO reduction, with an emphasis on masstransfer and the kinetic model of Fe(III)EDTA andFe(II)EDTA-NO reduction by immobilized bacteria. It wasfound that Fe(II)EDTA-NO had a strong inhibiting effect,but Fe(II)EDTA had no effect, on Fe(III)EDTA reduction. Fe(II)EDTA accelerated Fe(II)EDTA-NO reduction, whereasFe(III)EDTA had no effect. This showed that the use of thetwo stages of regeneration was necessary. Moreover, theeffect of internal diffusion on Fe(III)EDTA and Fe(II)EDTANOreduction could be neglected, and the rate-limiting stepwas the bioreduction process. The reduction of Fe(III)EDTAand Fe(II)EDTA-NO using immobilized bacteria wasdescribed by a first-order kinetic model. Bioreduction cantherefore be enhanced by increasing the cell density in themagnetic chitosan microspheres.
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Jingcui Yu,Songbin Fu,Peng Liu,Xiaobo Cui,Yu Sui,Guohua Ji,Rongwei Guan,Donglin Sun,Wei Ji,Fangli Liu,An Liu,Yuzhen Zhao,Yang Yu,Yan Jin,Jing Bai,Jingshu Geng,Yingwei Xue,Jiping Qi,Ki-Young Lee 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.1
Previously, we identified 3 overlapping regions showing loss of heterozygosity (LOH, R_1-R_3 from 11 to 30 cM) on chromosome 17 in 45 primary gastric cancers (GCs). The data indicated the presence of tumor suppressor genes (TSGs) on chromosome 17 involved in GC. Among the putative TSGs in these regions, HIC1 (in SR_1) and TOB1 (in SR_3) remain to be examined in GC. By immunohistochemistry (IHC), methylation-specific PCR (MSP) and western blot, we evaluated the expression and regulation status for HIC1 and TOB1 protein in GC. We narrowed down the deletion intervals on chromosome 17 and defined five smaller LOH subregions, SR_1-SR_5 (0.54 to 3.42 cM), in GC. We found that HIC1 had downregulated expression in 86% (91/106) and was methylated in 87% (26/30) of primary GCs. Of the primary GCs showing downregulation of HIC1 protein, 75% (18/24) had methylated HIC1 gene. TOB1 was either absent or expressed at reduced levels in 75% (73/97) of the GC samples. In addition, a general reduction was found in total and the ratio of unphosphorylated to phosphorylated TOB1 protein levels in the differentiated GC cell lines. Further analysis revealed significant simultaneous downregulation of both HIC1 and TOB1 protein in GC tissue microarray samples (67%, 52/78) and in primary GCs (65%, 11/17). These results indicate that silencing of HIC1 and TOB1 expression is a common occurrence in GC and may contribute to the development and progression of the disease.
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