Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis

Guohong Li,Yupeng Yin,Jiong Chen,Yanle Fan,Juhong Ma,Yingxue Huang,Chen Chen,Pengxiu Dai,Shulin Chen,Shanting Zhao 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1

Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migration of both early- and late-born neurons during mouse corticogenesis. Moreover, Cotl1 overexpression delayed, rather than blocked, neuronal migration in late-born neurons. Cotl1 expression disturbed the morphology of migrating neurons, lengthening the leading processes. This study is the first to investigate the function of Cotl1, and the results indicate that Cotl1 is involved in the regulation of neuronal migration and morphogenesis.

Composite Materials : SOLIDIFICATION PROCESSLNG OF METAL MATRIX COMPOSITES AND ITS EFFECTS ON COMPOSITES MECHANICAL PROPERTIES

Guohong Xie Songchun Li,Wuxian Wang,Renjie Wu 대한금속재료학회 ( 구 대한금속학회 ) 1995 Advanced Material and Processing Vol.3 No.-

Association of Perceived Stress With Depression Among Vaccinated Healthcare Workers During the SARS-CoV-2 Variant Outbreak: The Mediating Role of Compassion Fatigue

Cai Xue,Li Guohong,Feng Haixia,Wang Xiaoyan,He Lijun,Luo Dan,Xu Cuirong,Huang Yingzi,Qiu Shanhu 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.4

Objective Depression was common during coronavirus disease-2019 (COVID-19) pandemic, while the association of perceived stress with depression among vaccinated healthcare workers has not been investigated. This study aimed to address this issue.Methods We included a total of 898 fully vaccinated healthcare workers during the outbreak of severe acute respiratory syndrome coronavirus 2 Delta variant in Nanjing, 2021. Depression was ascertained by Patient Health Questionnaire-9, with a cut-off score of ≥5 indicative of mild-to-severe depression. Perceived stress, resilience, and compassion fatigue were assessed by Perceived Stress Scale-10, Resilience Scale-25, and Professional Quality of Life Scale version-5, respectively. Logistic regression analyses were used to estimate the odds ratio (OR) and 95% confidence interval (CI), along with subgroup and mediation analyses.Results The prevalence of mild-to-severe depression was 41.1% in vaccinated healthcare workers. The odd of mild-to-severe depression was increased with higher perceived stress. Compared with vaccinated healthcare workers with the lowest tertile of perceived stress, those with the highest tertile had increased odds of mild-to-severe depression by 120% (OR 2.20, 95% CI 1.46 to 3.31) after multivariable-adjustment. However, perceived stress was not associated with mild-to-severe depression in vaccinated healthcare workers with strong resilience, but was in those with weak resilience (p_interaction=0.004). Further analysis showed that compassion fatigue mediated the relationship between perceived stress and mild-to-severe depression, with a mediating effect of 49.7%.Conclusion Perceived stress was related to an increased odd of mild-to-severe depression in vaccinated healthcare workers during COVID-19 pandemic, and this relationship might be explained by compassion fatigue.

Mitogen-activated protein kinase 3 and 6 regulate <i>Botrytis cinerea</i>-induced ethylene production in Arabidopsis

Han, Ling,Li, Guo-Jing,Yang, Kwang-Yeol,Mao, Guohong,Wang, Ruigang,Liu, Yidong,Zhang, Shuqun Blackwell Publishing Ltd 2010 The Plant journal Vol.64 No.1

<P>Summary</P><P>Plants challenged by pathogens, especially necrotrophic fungi such as <I>Botrytis cinerea</I>, produce high levels of ethylene. At present, the signaling pathways underlying the induction of ethylene after pathogen infection are largely unknown. MPK6, an Arabidopsis stress-responsive mitogen-activated protein kinase (MAPK) was previously shown to regulate the stability of ACS2 and ACS6, two type I ACS isozymes (1-amino-cyclopropane-1-carboxylic acid synthase). Phosphorylation of ACS2 and ACS6 by MPK6 prevents rapid degradation of ACS2/ACS6 by the 26S proteasome pathway, resulting in an increase in cellular ACS activity and ethylene biosynthesis. Here, we show that MPK3, which shares high homology and common upstream MAPK kinases with MPK6, is also capable of phosphorylating ACS2 and ACS6. In the <I>mpk3</I> mutant background, ethylene production in gain-of-function <I>GVG-NtMEK2</I><SUP><I>DD</I></SUP> transgenic plants was compromised, suggesting that MPK6 and MPK3 function together to stabilize ACS2 and ACS6. Using a liquid-cultured seedling system, we found that <I>B. cinerea</I>-induced ethylene biosynthesis was greatly compromised in <I>mpk3/mpk6</I> double mutant seedlings. In contrast, ethylene production decreased only slightly in the <I>mpk6</I> single mutant and not at all in the <I>mpk3</I> single mutant, demonstrating overlapping roles for these two highly homologous MAPKs in pathogen-induced ethylene induction. Consistent with the role of MPK3/MPK6 in the process, mutation of <I>ACS2</I> and <I>ACS6</I>, two genes encoding downstream substrates of MPK3/MPK6, also reduced <I>B. cinerea</I>-induced ethylene production. The residual levels of ethylene induction in the <I>acs2/acs6</I> double mutant suggest the involvement of additional ACS isoforms, possibly regulated by MAPK-independent pathway(s).</P>

Endophytic Fungus Trichothecium roseum LZ93 Antagonizing Pathogenic Fungi In Vitro and Its Secondary Metabolites

XiaoMei Zhang,GuoHong Li,Juan Ma,Ying Zeng,PeiJi Zhao,WeiGuang Ma 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

The endophytic fungus Trichothecium roseum LZ93 from Maytenus hookeri was found to antagonize other pathogenic fungi in vitro. To identify which compound contributed substantially to the antagonism, we fermented the strain and purified its fermentation products. Eleven compounds were obtained, including two trichothecenes, five rosenonolactones, two cardiotonic cyclodepsipeptides, and two sterols. Compound 11β-hydroxyrosenonolactone (1) was assigned according to 1D and 2D-NMR data for the first time. At the same time, the 1H and 13C-NMR assignments for 6β-hydroxyrosenonolactone (2) were revised. Of all of them, only trichothecin (6) showed strong antifungal activity. Based on our observations of the antagonistic activity and the other experimental results, we suggest that the antifungal compound trichothecin was the main contributor to the antagonistic action of T. roseum LZ93.

EEE-GSM : End-to-End Encryption Scheme over GSM System

Mohammed Ramadan,Guohong Du,Fagen Li,Chun Xiang Xu 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.6

GSM system is widely used by hundreds of millions of people. In fact there is no encryption scheme provides the reasonable security level (user-to-user encryption), it’s just provide the Air-interface encryption i.e. between the mobile station and the base station. Furthermore, there are other wireless links are vulnerable to attacks. It is therefore of great importance to provide reasonable security techniques to ensure the privacy of the mobile users especially circuit switching-based services, as well as prevent unauthorized use of the service. In this paper, a new approach is proposed to provide end-to-end encryption for the GSM system (EEE-GSM). This is achieved by using CL-PKC with some modifications and follow some assumptions in GSM system architecture in order to make the scheme compliant to the GSM cellular system. However, the proposed scheme not only efficient due to end-to-end security, but can also provide a secure system against IMSI catcher, man-in-the-middle, and replay attacks.

The fecal microbiota composition of boar Duroc, Yorkshire, Landrace and Hampshire pigs

Xiao, Yingping,Li, Kaifeng,Xiang, Yun,Zhou, Weidong,Gui, Guohong,Yang, Hua Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10

Objective: To investigate the effect of host genetics on gut microbial diversity, we performed a structural survey of the fecal microbiota of four purebred boar pig lines: Duroc, Landrace, Hampshire, and Yorkshire. Methods: The V3-V4 regions of the 16S rRNA genes were amplified and sequenced. Results: A total of 783 operational taxonomic units were shared by all breeds, whereas others were breed-specific. Firmicutes and Bacteroidetes dominated the majority of the fecal microbiota; Clostridia, Bacilli, and Bacteroidia were the major classes. Nine predominant genera were observed in all breeds and eight of them can produce short-chain fatty acids. Some bacteria can secrete cellulase to aid fiber digestion by the host. Butyric, isobutyric, valeric, and isovaleric acid levels were highest in Landrace pigs, whereas acetic and propionic acid were highest in the Hampshire breed. Heatmap was used to revealed breed-specific bacteria. Principal coordinate analysis of fecal bacteria revealed that the Landrace and Yorkshire breeds had high similarity and were clearly separated from the Duroc and Hampshire breeds. Conclusion: Overall, this study is the first time to compare the fecal microbiomes of four breeds of boar pig by high-throughput sequencing and to use Spearman's rank correlation to analyze competition and cooperation among the core bacteria.

Trastuzumab Biosimilar (HLX02), Pertuzumab Plus Chemotherapy in Patients with HER2-Positive Metastatic Breast Cancer after Progression of Trastuzumab: A Prospective, Phase II Study

Ruyan Zhang,Xiaoran Liu,Guohong Song,Yan Zhang,Huiping Li 대한암학회 2024 Cancer Research and Treatment Vol.56 No.3

Purpose This study aims to evaluate the efficacy and safety of trastuzumab biosimilar (HLX02) in combination with pertuzumab and chemotherapy in patients with human epidermal growth factor receptor 2 (HER2)–positive metastatic breast cancer (MBC) after progression of trastuzumab. Materials and Methods In this prospective, single-arm, phase II study, patients with HER2-positive MBC after progression of trastuzumab received pertuzuamb, HLX02, and chemotherapy in Beijing Cancer Hospital from March 2020 to December 2022. The primary endpoint was progression-free survival (PFS), and secondary endpoints included objective response rate (ORR), disease control rate (DCR), overall survival (OS), and safety. The study was registered with ClinicalTrials.gov (NCT05188495). Results A total of 45 patients were included in this study. Twelve patients (26.7%) were treated in second-line and 33 patients (73.3%) were in third-line and later setting. Eighty percent and 15.5% patients had previously received pyrotinib/lapatinib and T-DM1, respectively. With a median follow-up of 24.4 months (range, 1.2 to 43.9 months), the median PFS was 7.6 months (95% confidence interval, 4.3 to 10.9), OS was not reached, the ORR was 31.1%, and DCR was 91.1%. The treatment was well tolerated. Conclusion The combination of trastuzumab biosimilar HLX02, pertuzumab, and chemotherapy exhibited promising efficacy and a favorable safety profile as second- and beyond-line treatment in HER2-positive MBC.

Effects of Sulfur Sources on the Microstructure and Photocatalytic Activity of ZnIn2S4 Microspheres

Shunsheng Chen,Shaozhen Li,Liangbin Xiong,Guohong Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.7

Two kinds of hexagonal ZnIn2S4 samples have been synthesized by applying different sulfur sources of cysteine and thioacetamide (TAA) in a hydrothermal process. The two samples have the same crystal structure but different visible-light absorption and photodegradation activity of methyl orange. The results of Brunauer–Emmett–Teller (BET) and scanning electron microscopy (SEM) revealed that, compared with ZnIn2S4 prepared using cysteine as sulfur source, ZnIn2S4 samples prepared using TAA possess much higher specific surface areas, smaller particle size and rather thinner nanosheets, which not only can enhance the adsorption of methyl orange on the surface of catalysts but also improve the transport efficiency of photogenerated charge carries.

Terbium Tetra-Sulfosalicylate Complex as Quantitative Luminescent Sensing of Chromate Ions with High Selectivity and Sensitivity

Wei Yang,Jinfeng Xia,Guohong Zhou,Tianxi Hu,Danwang Ye,Danyu Jiang,Qiang Li 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.5

In this study, a terbium(?) complex, Tb(SSA)4 (SSA?=?sulfosalicylate), was synthesized and employed to detect toxic chromate anion (CrO42?) as a visually identifiable probe. Luminescence investigations confirmed that the Tb complex is an excellent probe with high selectivity, high sensitivity (I0/I100 ?M = 9.5), and a low detection limit (2.8???10?8 M), targeting CrO42? in aqueous solution. The simple fluorescence-based probe can be quickly and easily prepared, and enables the reliable, visual detection of CrO42? ion in daily applications.