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- 저자 : Guo An Bi (검색결과 35 건)
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Zu-Guo Zhao,Yun Mei Yu,Bi Yu Xu,Shuang-Shuang Yan,Jun-Fa Xu,Fang Liu,Guo-Ming Li,Yuan Lin Ding,Shu Qing Wu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2
In Pseudomonas aeruginosa, a quorum sensing (QS) system regulates the expression of many virulence factors. N-acyl homoserine lactone (HSL) is the signal molecule of QS system. In order to find a novel HSL binder to interfere with QS signaling and to attenuate P. aeruginosa virulence, an amino lactam surrogate (ALS) of HSL was used as a target to screen HSL aptamers with the technique of systematic evolution of ligands by exponential enrichment (SELEX). Eight HSL aptamers with high affinities for 3O-C12-HSL (20 nM ≤ Kd < 35 nM) or C4-HSL (25 nM < Kd < 50 nM) were finally obtained. In vitro QS-inhibiting study of P. aeruginosa showed that HSL aptamers could inhibit virulence in a dose-dependent manner. ALSap-8 which bound C4-HSL primarily acted on the rhl system and inhibited the secretion of pyocyanin. ALSap-5 which bound 3O-C12-HSL not only showed strong inhibitory activity on biofilm formation as well as secretions of LasA protease and LasB elastase, but also reduced pyocyanin secretion. Since the las system is capable of activating the rhl system mildly, we speculated that ALSap-5 can simultaneously interfere with the las and rhl systems. High-affinity aptamers against HSL in this study are novel QS and virulence-inhibitors, and may have potential as drug candidates for the treatment of P. aeruginosa infection.
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Guo-Liang Wang,Shaohong Qu,Zhengjie Yuan,Yu Zhang,Guojuan Xu,Dongling Bi,Haiyan Qu,Xiaowei Zou,Xiaoqing Gao,Haihe Yang,Haiyan He,Xuli Wang,Jiandong Bao,Shimin Zuo,Xuebiao Pan,Bo Zhou 한국식물학회 2018 Journal of Plant Biology Vol.61 No.3
Rice sheath blight (SB), caused by Rhizoctoniasolani (R. solani), is a major threat to rice productionworldwide. The molecular mechanisms of the SB resistancein rice are poorly understood. The transcriptomes of the SBresistantrice cultivar YSBR1 and the susceptible cultivarLemont were analyzed after R. solani infection. A total of7624 differentially expressed genes (DEGs) were identifiedat one or more timepoints in a cultivar. 5526 and 5618 DEGswere differentially expressed in Lemont and YSBR1,respectively. YSBR1 exhibited stronger and earlier transcriptionalresponse to R. solani than Lemont. Gene ontology enrichmentanalysis revealed that genes that encode cell wall-modifyingand glycosyl-degrading enzymes or anti-microbial proteinswere specifically induced in YSBR1 at 6 hpi. MapMananalysis revealed that more DEGs related with cell wall, β-glucanses, respiratory burst, phenylpropanoids and ligninwere highly induced by R. solani in YSBR1 than in Lemont. The results also showed that receptor-like kinases and jasmonicacid signaling may play important roles in host resistance toR. solani. This study highlights potential candidate genes andsignaling pathways involved in rice sheath resistance and canhelp to further clarify the mechanistic events underlyingresistance and susceptibility to R. solani.
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Guo, Cun-Li,Yang, Xiu-Hua,Cheng, Wen,Xu, Yi,Li, Jie-Bing,Sun, Yi-Xin,Bi, Yu-Mei,Zhang, Lei,Wang, Qiu-Cheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6
Aims: Dysfunction of the host immune system in cancer patients can be due to a number of factors, including lymphocyte apoptosis. Several studies showed that $Foxp3^+T$ cells take part in inducing this process by expressing FasL in tumor patients. However, the relationship between apoptosis, $CD8^+T$ cells and $Foxp3^+T$ cells in HCC patients is still unclear. The present study was designed to investigate the correlation between apoptosis levels and Fas/FasL expression in $CD8^+T$ lymphocytes and $Foxp3^+T$ cells in patients with HCC. Methods: $CD8^+T$ cells and $CD3^+Foxp3^+T$ cells were tested from peripheral blood of HCC patients and normal controls and subjected to multicolor flow cytometry. The expression of an apoptosis marker (annexin V) and the death receptor Fas in $CD8^+T$ cells and FasL in $CD3^+Foxp3^+T$ cells were evaluated. Serum TGF-${\beta}1$ levels in patients with HCC were measured by enzyme-linked immunosorbent assay. The relationship between apoptosis and Fas expression, as well as FasL expression in $CD3^+Foxp3^+T$ cells was then evaluated. Results: The frequency of $CD8^+T$ cells binding annexin V and Fas expression in $CD8^+T$ cells, were all higher in HCC patients than normal controls and the proportion of apoptotic $CD8^+T$ cells correlated with their Fas expression. Serum TGF-${\beta}1$ levels correlated inversely with $CD3^+Foxp3^+T$ cells. Conclusions: Fas/FasL interactions might lead to excessive turnover of $CD8^+T$ cells and reduce anti-tumor immune responses in patients with HCC. Further investigations of apoptosis induction in $Fas^+CD8^+T$ cells in vitro are required.
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Wei, Guo,Nie, Ming-Ming,Shen, Xiao-Jun,Xue, Xu-Chao,Ma, Li-Ye,Du, Cheng-Hui,Wang, Shi-Liang,Bi, Jian-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
Objective: To observe local and systemic toxicity after sustained-release 5-fluorouracil (5-Fu) implantation in canine peritoneum and para-aortic abdominalis and the changes of drug concentration in the local implanted tissue with time. Methods: 300 mg sustained-release 5-Fu was implanted into canine peritoneum and para-aorta abdominalis. Samples were taken 3, 5, 7 and 10 days after implantation for assessment of changes and systemic reactions. High performance liquid chromatography was applied to detect the drug concentrations of peritoneal tissue at different distances from the implanted site, lymphatic tissue of para-aortic abdominalis, peripheral blood and portal venous blood. Results: 10 days after implantation, the drug concentrations in the peritoneum, lymphatic tissue and portal vein remained relatively high within 5 cm of the implanted site. There appeared inflammatory reaction in the local implanted tissue, but no visible pathological changes such as cell degeneration and necrosis, and systemic reaction like anorexia, nausea, vomiting and fever. Conclusions: Sustained-release 5-Fu implantation in canine peritoneum and para-aortic abdominalis can maintain a relatively high tumour-inhibiting concentration for a longer time in the local implanted area and portal vein, and has mild local and systemic reactions. Besides, it is safe and effective to prevent or treat recurrence of gastrointestinal tumours and liver metastasis.
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Design and properties analysis of total internal reflection gratings for pulse compressor at 1053 nm
Qunyu Bi,Jiangjun Zheng,Ailin Guo,Meizhi Sun,Jianpeng Wang,Fuling Zhang,Qingwei Yang,Xinglong Xie,Zunqi Lin 한국물리학회 2011 Current Applied Physics Vol.11 No.1
High-efficiency compression gratings based on total internal reflection (TIR) are promising alternatives of compressor gratings because of their high diffraction efficiency, potential high damage resistant ability,and compact structure. Dependence of the 1 order diffraction efficiencies on grating parameters is analyzed for TE- and TM-polarized incident light of 1053 nm at Littrow angle, which is calculated by using the rigorous coupled-wave analysis. A more intuitional view on the relation is offered through three-dimensional slicing figures instead of two-dimensional ones. The performances of high-efficiency gratings are compared and regarded as criteria for further choices, including spectral bandwidth, angle bandwidth, dispersion, and intensity distribution. For TE- and TM-irradiations, similar spectral bandwidth and angle bandwidth can be achieved by different grating parameters. However, the computer simulation result on the intensity distributions of the two polarized waves shows that such design should be used under the illumination of TE-polarized wave for lower intensity enhancement ratio,which is an important factor related to the gratings’ damage threshold.
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Yannan Bi,Zhipeng Shen,Qun Wang,Haomiao Yu,Chen Guo 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12
This article investigates the adaptive tracking control problem for the marine surface vessels (MSVs) with unknown uncertainties and multiple constraints. Firstly, a novel prescribed performance-based time-varying asystematic barrier Lyapunov function (PP-TABLF) is proposed to control the MSVs to navigate in a variable narrow waterway and to improve the transient performance of MSVs. Secondly, the predictor-based radial basis function neural networks (P-RBFNNs) are developed to approximate the system uncertainties and external disturbances. Specifically, the tracking errors of the RBFNNs are estimated in advance, and the prediction errors are utilized to update the RBFNNs and improve the estimation precision. The command filter and the idea of the recursive sliding mode are integrated with the control law to limit the amplitude of the virtual control signals and to reduce the computational burden. With the proposed control scheme, tracking errors will not override the prescribed performance ranges, and the control force will not be violated in the presence of total unknown uncertainties. Finally, the semi-global uniformly ultimate boundedness of the system is guaranteed by the proposed control scheme, and the simulation results are given to further demonstrate the effectiveness of the proposed approach.
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LDMOS with Variable-k Dielectric for Improved Breakdown Voltage and Specific On-resistance
Songnan Guo,Junji Cheng,Xing Bi Chen 대한전자공학회 2019 Journal of semiconductor technology and science Vol.19 No.5
A silicon-on-insulator (SOI) lateral double-diffused MOSFET (LDMOS) with variable-k dielectric is proposed, which features three-section high-k dielectrics placed over the drift region. Beneficial from the modulation, by high-k dielectrics, on electric fluxes in the drift region, the electric field strength is locally enhanced, and has a more uniform distribution along the surface of the drift region, which an increment of the breakdown voltage (BV) ascribes to. Moreover, due to the electric fluxes prefer to flow towards and through a high-k dielectric, an increase in the dose of the drift region is thus possible to reduce the specific on-resistance (Ron,sp), since the extra electric fluxes caused by those increased impurities would flow, upwards, into the high-k dielectric, and barely make contributions to the x-component of the surface electric field, which indicates that the BV would not be affected. Based on a comparison with the conventional LDMOS, the BV of the proposed LDMOS increased by 31% with a decrease of 13% in the Ron,sp. The figure of merit (FOM) increased approximately 2 times.
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Quanxin Bi,Bin Guo,Dongxu Zhang,Wenbin Guan 한국유전학회 2015 Genes & Genomics Vol.37 No.3
Xanthoceras sorbifolium is an economicallyimportant woody plant in China. Plant microRNAs (miRNAs)play a vital role in plant development. We usedSolexa technology to sequence a small RNA library fromthe buds, young and mature leaves, flowers, and developingseeds (10, 30, and 60 days after pollination) of X. sorbifoliumto identify conserved and novel miRNAs. Sequenceanalysis identified 49 conserved miRNAs belonging to 19miRNA families, as well as four novel miRNAs. An analysisof 10 miRNA sequences by qRT-PCR showed that allsequences were expressed and that they were dynamicallyregulated in different tissues. Finally, seven targets of thenovel miRNAs were predicted. This study opens up a newavenue for understanding the roles of miRNAs in X. sorbifolium.
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Wang, Bi,Yu, Lei,Yang, Guo-Zhen,Luo, Xin,Huang, Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Objective: To explore the application of multiplex nested methylated specific polymerase chain reaction (PCR) in the early diagnosis of epithelial ovarian carcinoma (EOC). Materials and Methods: Serum and fresh tissue samples were collected from 114 EOC patients. RUNX3, TFPI2 and OPCML served as target genes. Methylation levels of tissues were assessed by multiplex nested methylated specific PCR, the results being compared with those for carcinoma antigen 125 (CA125). Results: The serum free deoxyribose nucleic acid (DNA) methylation spectrum of EOC patients was completely contained in the DNA spectrum of cancer tissues, providing an accurate reflection of tumor DNA methylation conditions. Serum levels of CA125 and free DNA methylation in the EOC group were evidently higher than those in benign lesion and control groups (p<0.05). Patients with early EOC had markedly lower serum CA125 than those with advanced EOC (p<0.05), but there was no significant difference in free DNA methylation (p>0.05). The sensitivity, specificity and positive predicative value (PPV) of multiplex nested methylated specific PCR were significantly higher for detection of all patients and those with early EOC than those for CA125 (p<0.05). In the detection of patients with advanced EOC, the PPV of CA125 detection was obviously lower than that of multiplex nested methylated specific PCR (p>0.05), but there was no significant difference in sensitivity (p>0.05). Conclusions: Serum free DNA methylation can be used as a biological marker for EOC and multiplex nested methylated specific PCR should be considered for early diagnosis since it can accurately determine tumor methylation conditions.
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