The Design and Realization of English Aid Input Method for Chinese

BaoSheng Yin,DongFeng Cai,GuiPing Zhang 한국어정보학회 2002 한국어정보학 Vol.7ㆍ8 No.-

Fixed switching frequency strategy for finite-control-set model predictive control based on cost function reconstruction

Lei, Yanxiong,Du, Guiping,Zhang, Yuhan,Li, Tuhuan The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.6

Considering the spread spectrum problem of finite-control-set model predictive control (FCS-MPC), a fixed switching frequency strategy based on cost function reconstruction is proposed in this paper. It achieves a fixed switching frequency by selecting the optimal voltage vector through the redefined cost function. A frequency coefficient is added to this to change the selecting area of the optimal voltage vector. The proposed strategy reshapes the spectrum of the inductor current and focuses the harmonics near the switching frequency so that the design of the output LC filter is easier. Without adding any modulators or greatly increasing the number of computations, the proposed strategy reserves the excellent dynamic performance of the FCS-MPC and is convenient in terms of implementation. Finally, a 1 kW single-phase inverter is built and experiments are conducted. Experimental results demonstrate that the proposed control strategy realizes a fixed switching frequency. In addition, it has a comparative dynamic response speed and number of calculations when compared to dead-beat-based FCS-MPC. These features are beneficial to its application.

pH-sensitive drug controlled release core/shell fibers fabricated by combination of electrospinning and photopolymerization

Xiao-lei Zhu,Hongwei Zhang,Jun Nie,Guiping Ma 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

pH-sensitive drug loaded core shellfibers were fabricated by a combination of electrospinning and UVphoto-polymerization. Combretastatin A4 (CA4) was selected as the model drug loaded in PLA to test thepH-sensitivity property of the core shellfibers. The morphology of thefibers was studied by scanningelectron microscopy (SEM), and the core shell structure of thefibers was confirmed by transmissionelectron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The drug release assay wastested via the UV–vis spectrophotometer, and the pH-sensitivity of core shellfibers was tested by drugrelease assay under pH 5.0 and 7.4.

Three-dimensional Printed Mg-Doped β-TCP Bone Tissue Engineering Scaffolds: Effects of Magnesium Ion Concentration on Osteogenesis and Angiogenesis In Vitro

Yifan Gu,Jing Zhang,Xinzhi Zhang,Guiping Liang,Tao Xu,Wei Niu 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.4

BACKGROUND: Three-dimensional (3D) printed bone tissue engineering scaffolds have been widely used in research and clinical applications. b-TCP is a biomaterial commonly used in bone tissue engineering to treat bone defects, and its multifunctionality can be achieved by co-doping different metal ions. Magnesium doping in biomaterials has been shown to alter physicochemical properties of cells and enhance osteogenesis. METHODS: A series of Mg-doped TCP scaffolds were manufactured by using cryogenic 3D printing technology and sintering. The characteristics of the porous scaffolds, such as microstructure, chemical composition, mechanical properties, apparent porosity, etc., were examined. To further study the role of magnesium ions in simultaneously inducing osteogenesis and angiogenesis, human bone marrow mesenchymal stem cells (hBMSCs) and human umblical vein endothelial cells (HUVECs) were cultured in scaffold extracts to investigate cell proliferation, viability, and expression of osteogenic and angiogenic genes. RESULTS: The results showed that Mg-doped TCP scaffolds have the advantages of precise design, interconnected porous structure, and similar compressive strength to natural cancellous bone. hBMSCs and HUVECs exhibit high proliferation rate, cell morphology and viability in a certain amount of Mg2?. In addition, this concentration of magnesium can also increase the expression levels of osteogenic and angiogenic biomarkers. CONCLUSION: A certain concentration of magnesium ions plays an important role in new bone regeneration and reconstruction. It can be used as a simple and effective method to enhance the osteogenesis and angiogenesis of bioceramic scaffolds, and support the development of biomaterials and bone tissue engineering scaffolds.

Functional Analysis of a Subtilisin-like Serine Protease Gene from Biocontrol Fungus Trichoderma harzianum

Haijuan Fan,Zhihua Liu,Rongshu Zhang,Na Wang,Kai Dou,Gulijimila Mijiti,Guiping Diao,Zhiying Wang 한국미생물학회 2014 The journal of microbiology Vol.52 No.2

The subtilisin-like serine protease gene ThSS45 has beencloned from Trichoderma harzianum ACCC30371. Its codingregion is 1302 bp in length, encoding 433 amino acids,with a predicted protein molecular weight of 44.9 kDa andpI of 5.91. ThSS45 was shown by RT-qPCR analysis to bedifferentially transcribed in response to eight different treatments. The transcription of ThSS45 was up-regulated whengrown in mineral medium, under carbon starvation, andnitrogen starvation, and in the presence of 1% root powder,1% stem powder, and 1% leaf powder derived from Populusdavidiana × P. bolleana (Shanxin poplar) aseptic seedlings. The highest increase in transcription approached 3.5-foldthat of the control at 6 h under induction with 1% poplarroot powder. The transcription of ThSS45 was also slightlyup-regulated by 1% Alternaria alternata cell wall and 5% A. alternata fermentation liquid. Moreover, the analyses of codingand promoter regions of ThSS45 homologs indicatedthat serine protease may be involved in both mycoparasitismand antibiotic secretion. ThSS45 was cloned into the pGEX-4T-2 vector and then expressed in Escherichia coli BL21. Therecombinant protein, with an expected molecular weight ofapproximately 69 kDa, was then purified. When transformantBL21-ss was induced with 1 mM IPTG for 6 h, thepurified protease activity reached a peak of 18.25 U/ml atpH 7.0 and 40°C. In antifungal assays the purified proteaseobviously inhibited the growth of A. alternata mycelia.

Identification of Histone Deacetylase 2 as a Functional Gene for Skeletal Muscle Development in Chickens

Shahjahan, Md.,Liu, Ranran,Zhao, Guiping,Wang, Fangjie,Zheng, Maiqing,Zhang, Jingjing,Song, Jiao,Wen, Jie Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.4

A previous genome-wide association study (GWAS) exposed histone deacetylase 2 (HDAC2) as a possible candidate gene for breast muscle weight in chickens. The present research has examined the possible role of HDAC2 in skeletal muscle development in chickens. Gene expression was measured by quantitative polymerase chain reaction in breast and thigh muscles during both embryonic (four ages) and post-hatch (five ages) development and in cultures of primary myoblasts during both proliferation and differentiation. The expression of HDAC2 increased significantly across embryonic days (ED) in breast (ED 14, 16, 18, and 21) and thigh (ED 14 and 18, and ED 14 and 21) muscles suggesting that it possibly plays a role in myoblast hyperplasia in both breast and thigh muscles. Transcript abundance of HDAC2 identified significantly higher in fast growing muscle than slow growing in chickens at d 90 of age. Expression of HDAC2 during myoblast proliferation in vitro declined between 24 h and 48 h when expression of the marker gene paired box 7 (PAX7) increased and cell numbers increased throughout 72 h of culture. During induced differentiation of myoblasts to myotubes, the abundance of HDAC2 and the marker gene myogenic differentiation 1 (MYOD1), both increased significantly. Taken together, it is suggested that HDAC2 is most likely involved in a suppressive fashion in myoblast proliferation and may play a positive role in myoblast differentiation. The present results confirm the suggestion that HDAC2 is a functional gene for pre-hatch and post-hatch (fast growing muscle) development of chicken skeletal muscle.

Tumor Promoting Function of DUSP10 in Non-Small Cell Lung Cancer Is Associated With Tumor-Promoting Cytokines

Xing Wei,Chin Wen Png,Madhushanee Weerasooriya,Heng Li,Chenchen Zhu,Guiping Chen,Chuan Xu,Yongliang Zhang,Xiaohong Xu 대한면역학회 2023 Immune Network Vol.23 No.4

Lung cancer, particularly non-small cell lung cancer (NSCLC) which contributes more than 80% to totally lung cancer cases, remains the leading cause of cancer death and the 5-year survival is less than 20%. Continuous understanding on the mechanisms underlying the pathogenesis of this disease and identification of biomarkers for therapeutic application and response to treatment will help to improve patient survival. Here we found that a molecule known as DUSP10 (also known as MAPK phosphatase 5) is oncogenic in NSCLC. Overexpression of DUSP10 in NSCLC cells resulted in reduced activation of ERK and JNK, but increased activation of p38, which was associated with increased cellular growth and migration. When inoculated in immunodeficient mice, the DUSP10-overexpression NSCLC cells formed larger tumors compared to control cells. The increased growth of DUSP10- overexpression NSCLC cells was associated with increased expression of tumor-promoting cytokines including IL-6 and TGFβ. Importantly, higher DUSP10 expression was associated with poorer prognosis of NSCLC patients. Therefore, DUSP10 could severe as a biomarker for NSCLC prognosis and could be a target for development of therapeutic method for lung cancer treatment.