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Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells
Gui-Hua Zhao,Lixin Zhang,Lisha Dai,Haozhi Xu,Chao Xu,Ting Xiao,Jin Li,Hui Sun,Beibei Zhou,Kun Yin 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.4
Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.
Five new species of Dicerapanorpa Zhong & Hua (Mecoptera, Panorpidae) from Yunnan, China
Gui-Lin Hu,Ji-Shen Wang,Bao-zhen Hua 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1
Five new scorpionfly species of the genus Dicerapanorpa Zhong and Hua, 2013 are described and illustrated from Yunnan Province, China. Dicerapanorpa macula sp. n. can be readily recognized by all branches of paramere parallel, basal and mesal branches extending well beyond basal process of gonostylus in male, and posterior arm of medigynium short and stout in female. Dicerapanorpa deqenensis sp. n. is characterized by basal branch of paramere as long as lateral branch and ventral valve of aedeagus nearly reaching apex of gonocoxite in male, and medigynium with paired auricular lateral processes in female. Dicerapanorpa tanae sp. n. is unique by genital bulb broad and spherical, basal branch of paramere short, and lateral branches curved convergently and parallel apically in male, and main plate of medigynium rounded in female. Dicerapanorpa tenuis sp. n. is distinguishable by hypovalve with largely expanded and inward curved apex and all branches of paramere reaching or exceeding apex of gonocoxite in male, and medigynium slender in female. Dicerapanorpa zhongdianensis sp. n. is characterized by basal branches of paramere parallel and mesal branches curved convergently in male, and main plate of medigynium subtriangular with a pair of auricular lateral processes in female. The species number of Dicerapanorpa is increased to thirteen.
( Gui Hua Lim ),( Young Jung Lee ),( Dong Young Choi ),( Sang Bae Han ),( Jae Kyung Jung ),( Bang Yeon Hwang ),( Dong Chul Moon ),( Young Soo Kim ),( Myung Koo Lee ),( Ki Wanoh ),( Heon Sang Jeong ),( 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
Neuroinflammation is implicated for amyloidogenesis. Sulfur compounds extracted from garlic have been shown to have anti-inflammatory properties. Previously, we have investigated that thiacremonone, a sulfur compound isolated from garlic has anti-inflammatory effects. To investigate thiacremonone`s potential effect on anti-neuroinflammation and anti-amyloidogenesis, 4 week old ICR mice were given different doses of thiacremonone (1, 3, and 10 mg/kg) in drinking water for 1 month and received intraperitoneal injection of lipopolysaccharide (LPS) (250 μg/kg/day) at last 7 days of treatment. Our data show thiacremonone decreased LPS-induced memory impairment, glial activation, pro-inflammatory mediators` expression, and amyloidogenesis. In an in vitro study, we obtained similar results, with thiacremonone (1, 2, and 5 μg/ml) effectively decreased LPS (1 μg/ml)-induced glial activation and inflammatory mediators generation which are implicated in amyloidogenesis. Our data also demonstrated that thiacremonone inhibited LPS-induced amyloidogenesis in cultured astrocytes and microglial BV-2 cells. NF-κB, a critical transcriptional factor regulating not only inflammation but also amyloid-β generation, was inhibited by thiacremonone via blocking of phosphorylation of IκBα in mice brain as well as cultured astrocytes and microglial BV-2 cells. These results indicated that the anti-inflammatory compound, thiacremonone, inhibited neuroinflammation and amyloidogenesis through inhibition of NF-κB activity, and thus could be applied for intervention of inflammation-related neurodegenerative disease including Alzheimer`s disease.
Gui-Hua Zhang,Jafar Ali,Sungho Lee,Dae-Wan Kim 한국디지털융합학회 2023 IJICTDC Vol.8 No.2
In recent years, the adoption of mobile CRM has become an efficient alternative in maintaining and improving performance at the firm level. This study focuses on system, service, and organizational characteristics and develops a research model based on an existing model of successful CRM development. The study hypothesizes about relationships between a number of m-CRM characteristic factors and m-CRM performance. These factors include the infrastructure readiness, system integration, location awareness, service accessibility, sector knowledge, and organizational learning. The results have important theoretical and practical implications, and the limitations of the study provide interesting avenues for future research.
Gui-Hua Zhao,Kun Yin,Wei-Xia Zhong,Ting Xiao,Qing-Kuan Wei,Yong Cui,Gong-Zhen Liu,Chao Xu,Hong-Fa Wang 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.6
Heishui county, located in northwest Sichuan province, southwestern China, is an endemic area of zoonotic visceral leishmaniasis (VL) and is the most intractable area. VL is never destroyed in it. Asymptomatic dogs (Leishmania parasites have been diagnosed but clinically healthy) are considered to be a potential reservoir host in zoonotic VL area, and most can lead to infection of individuals, that is a new challenge for controlling VL in humans. The present study aimed to assess the Leishmania infection rate of asymptomatic dogs in Heishui county. Total 105 asymptomatic domestic dogs were gathered from 4 districts in Heishui county to investigate the infection rate with serological and molecular methods based on ELISA and kinetoplast minicircle DNA(kDNA) PCR, respectively. Out of 105 dogs, 44 (41.9%) were positive by more than 1 method; 21 (20.0%) were positive by ELISA, and 30 (28.6%) were positive by kDNA-PCR. Our study showed that Leishmania infection of domestic dogs which is clinically healthy is prevalent in the studied district, and the asymptomatic dogs infected by Leishmania may be the primary reason for the prevalence of visceral leishmaniasis in the area.
Gui-Hua Quan,오세량,Hyuk-Hwan Song,Hyeong-Kyu Lee,Young-Won Chin 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.
Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq
( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.
Gui Hua Quan,Young Won Chin,Hyeong Kyu Lee,Sei Ryang Oh 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1
A simple and rapid purification method of deoxypodophyllotoxin from the crude methanol extract of rhizomes of Anthriscus sylvestris was established using high-speed counter-current chromatography. From the crude extract (288.9 mg), deoxypodophyllotoxin (8.