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- 저자 : Lisha Dai (검색결과 2 건)
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Jing, Xiaoli,Peng, Lisha,Dai, Kun 서울대학교 교육연구소 2021 Asia Pacific Education Review Vol.22 No.4
With the growing trend of globalization, an increasing number of Chinese students choose to study overseas. While researchers have conducted many studies to explore factors motivating these students to undertake higher education overseas, few studies have investigated Chinese students’ motivations to pursue secondary education abroad. To fll this research gap, we interviewed 20 Chinese secondary students and four Chinese parents to explore Chinese students’ motivations to pursue secondary education abroad by taking Canada as a study destination example. By adopting the push–pull model as the theoretical framework, we identify two macro-level push factors and three micro-level push factors that propel Chinese secondary students to study outside China. It also reveals fve pull factors at the macro-level and two pull factors at the micro-level that attract these students to select Canada as a study abroad destination. On the basis of identifying these push–pull factors, we further discuss the similarities and diferences of Chinese students’ motivations to pursue secondary education abroad and to undertake higher education overseas.
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Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells
Gui-Hua Zhao,Lixin Zhang,Lisha Dai,Haozhi Xu,Chao Xu,Ting Xiao,Jin Li,Hui Sun,Beibei Zhou,Kun Yin 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.4
Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.
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