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Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Zhao, Yongju,Wei, Hong,Wang, Yong,Wang, Lingbin,Yu, Mingju,Fan, Jingsheng,Zheng, Shuangyan,Zhao, Cong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (SMGT) in the goat were optimized. The standard sperm parameters and the ability to bind foreign genes were assessed to select suitable sperm donor bucks. A total of 134 oestrous does were divided into 4 groups and inseminated using different methods and sperm numbers. The does of Groups I to III were inseminated with fresh semen ($1-2\times10^{7}$ and $10^{6}$ sperm) or frozen-thawed semen ($10^{6}$ sperm), respectively, through conventional intra-cervical AI, and the does of Group IV with frozen-thawed semen ($10^{6}$ sperm) through intrauterine AI. Total genomic DNAs were extracted from ear biopsies of the offspring. The presence of $pEGFP-N_{1}$ DNA was screened by PCR and then by Southern blotting analysis. A total of 76 live kids were produced and 8 kids were tested transgene positive on the basis of agarose gel electrophoresis of the PCR-amplified fragment. Southern blotting analysis of the samples showed 5 positive kids. A transgenic ratio of 10.53% was detected using PCR and 6.58% using Southern blotting. The positive kid rate assayed by PCR and Southern blotting of frozen-thawed goat semen was 3.61% and 9.27% higher than that of untreated semen. The results show that transgenic goats can be produced efficiently by the method of artificial insemination using sperm cells to integrate the exogenous DNA and intrauterine insemination allowed low numbers of DNA-transfected spermatozoa to be used, with satisfactory fertility.
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Fan Yang,Xin Fan,Man Zhang,Chen Wang,Wei-Feng Zhao,Chang-Sheng Zhao 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
Polyacrylic acid/polyethersulfone (PAA/PES) capsules were adopted as templates to synthesize PAA/PES core–shell spheres, and the PES shell of spheres could be removed automatically by the swelling nature of the cross-linked PAA hydrogel core. By using the method, PAA hydrogel spheres were prepared with an ultrahigh ion exchange capacity of 12 meq g−1, and the spheres showed a high adsorption capacity for methylene blue. The PAA-polyacrylamide hydrogel spheres with dual adsorption ability and good mechanical property were also prepared. It was revealed that these two hydrogel spheres can be effectively utilized in the removal of environmental toxins.
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An efficient Video Dehazing Algorithm Based on Spectral Clustering
( Fan Zhao ),( Zao Yao ),( Xiaofang Song ),( Yi Yao ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.7
Image and video dehazing is a popular topic in the field of computer vision and digital image processing. A fast, optimized dehazing algorithm was recently proposed that enhances contrast and reduces flickering artifacts in a dehazed video sequence by minimizing a cost function that makes transmission values spatially and temporally coherent. However, its fixed-size block partitioning leads to block effects. The temporal cost function also suffers from the temporal non-coherence of newly appearing objects in a scene. Further, the weak edges in a hazy image are not addressed. Hence, a video dehazing algorithm based on well designed spectral clustering is proposed. To avoid block artifacts, the spectral clustering is customized to segment static scenes to ensure the same target has the same transmission value. Assuming that edge images dehazed with optimized transmission values have richer detail than before restoration, an edge intensity function is added to the spatial consistency cost model. Atmospheric light is estimated using a modified quadtree search. Different temporal transmission models are established for newly appearing objects, static backgrounds, and moving objects. The experimental results demonstrate that the new method provides higher dehazing quality and lower time complexity than the previous technique.
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Fast Encoder Design for Multi-view Video
( Fan Zhao ),( Kaiyang Liao ),( Erhu Zhang ),( Fangying Qu ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.7
Multi-view video coding is an international encoding standard that attains good performance by fully utilizing temporal and inter-view correlations. However, it suffers from high computational complexity. This paper presents a fast encoder design to reduce the level of complexity. First, when the temporal correlation of a group of pictures is sufficiently strong, macroblock-based inter-view prediction is not employed for the non-anchor pictures of B-views. Second, when the disparity between two adjacent views is above some threshold, frame-based inter-view prediction is disabled. Third, inter-view prediction is not performed on boundary macroblocks in the auxiliary views, because the references for these blocks may not exist in neighboring views. Fourth, finer partitions of inter-view prediction are cancelled for macroblocks in static image areas. Finally, when estimating the disparity of a macroblock, the search range is adjusted according to the mode size distribution of the neighboring view. Compared with reference software, these techniques produce an average time reduction of 83.65%, while the bit-rate increase and peak signal-to-noise ratio loss are less than 0.54% and 0.05dB, respectively.
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An efficient Video Dehazing Algorithm Based on Spectral Clustering
Zhao, Fan,Yao, Zao,Song, Xiaofang,Yao, Yi Korean Society for Internet Information 2018 KSII Transactions on Internet and Information Syst Vol.12 No.7
Image and video dehazing is a popular topic in the field of computer vision and digital image processing. A fast, optimized dehazing algorithm was recently proposed that enhances contrast and reduces flickering artifacts in a dehazed video sequence by minimizing a cost function that makes transmission values spatially and temporally coherent. However, its fixed-size block partitioning leads to block effects. The temporal cost function also suffers from the temporal non-coherence of newly appearing objects in a scene. Further, the weak edges in a hazy image are not addressed. Hence, a video dehazing algorithm based on well designed spectral clustering is proposed. To avoid block artifacts, the spectral clustering is customized to segment static scenes to ensure the same target has the same transmission value. Assuming that edge images dehazed with optimized transmission values have richer detail than before restoration, an edge intensity function is added to the spatial consistency cost model. Atmospheric light is estimated using a modified quadtree search. Different temporal transmission models are established for newly appearing objects, static backgrounds, and moving objects. The experimental results demonstrate that the new method provides higher dehazing quality and lower time complexity than the previous technique.
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Fan, Yong,Zhao, Ming-yuan,Ma, Lin,Zhao, Lan-yong Institute of Forest Science 2016 Journal of Forest Science Vol.32 No.1
Accessibility of urban green space, as an important index of evaluating the service level of urban green space, not only shows the resident's access to green space, but also reflects the environment quality of urban living. Since the quantity, area and space distribution of the patches in urban green space directly affect the function of urban green space and the quality of urban environment, the research on the accessibility of urban green space holds great significance to improve the service of urban green space and the living environment quality. Based on GIS software, this research uses the road network and the population distribution data of Nanjing's city proper to conduct quantitative analysis of the accessibility of park green space and the convenience for residents to travel under different forms of transportation. After the case analysis, the author tests the application of road network in studying the accessibility of urban park green space and proposes a GIS-based method to study the accessibility and the rational layout of urban green space.
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Zhao, Cheng-Xiao,Liu, Ming,Xu, Yong,Yang, Kuo,Wei, Dong,Shi, Xiao-Hong,Yang, Fan,Zhang, Yao-Guang,Wang, Xin,Liang, Si-Ying,Zhao, Fan,Zhang, Yu-Rong,Wang, Na-Na,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
Background: Evidence supporting an association between the 8q24 rs4242382-A polymorphism and prostate cancer (PCa) risk has been reported in North American and Europe populations, though data from Asian populations remain limited. We therefore investigated this association by clinical detection in China, and meta-analysis in Asian, Caucasian and African-American populations. Materials and Methods: Blood samples and clinical information were collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=335) and from age-matched normal controls (n=347). The 8q24 (rs4242382) gene polymorphism was genotyped by polymerase chain reaction-high-resolution melting analysis. We initially analyzed the associations between the risk allele and PCa and clinical covariates. A meta-analysis was then performed using genotyping data from a total of 1,793 PCa cases and 1,864 controls from our study and previously published studies in American and European populations, to determine the association between PCa and risk genotype. Results: The incidence of the risk allele was higher in PCa cases than controls (0.222 vs 0.140, $P=7.3{\times}10^{-5}$), suggesting that the 8q24 rs4242382-A polymorphism was associated with PCa risk in Chinese men. The genotypes in subjects were in accordance with a dominant genetic model (ORadj=2.03, 95%CI: 1.42-2.91, $Padj=1.1{\times}10^{-4}$). Presence of the risk allele rs4242382-A at 8q24 was also associated with clinical covariates including age at diagnosis ${\geq}65$ years, prostate specific antigen >10 ng/ml, Gleason score <8, tumor stage and aggressive PCa, compared with the non-risk genotype ($P=4.6{\times}10^{-5}-3.0{\times}10^{-2}$). Meta-analysis confirmed the association between 8q24 rs4242382-A polymorphism and PCa risk (OR=1.62, 95%CI: 1.39-1.88, $P=1.0{\times}10^{-5}$) across Asian, Caucasian and African American populations. Conclusions: The replicated data suggest that the 8q24 rs4242382-A variation might be associated with increased PCa susceptibility in Asian, Caucasian and African American populations. These results imply that this polymorphism may be a useful risk biomarker for PCa in multi-ethnic populations.
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Does H3K27M Mutation Impact Survival Outcome of High-Grade Spinal Cord Astrocytoma?
Fan Zhang,Lei Cheng,Ze Ding,Shengxi Wang,Xingang Zhao,Zijun Zhao,Cong Liang,Kun Wu,Dongao Zhang,Yinqian Wang,Tao Fan 대한척추신경외과학회 2023 Neurospine Vol.20 No.4
Objective: To evaluate the impact of H3K27M mutation in the prognosis of histological high-grade intramedullary astrocytoma. Methods: A total of 78 patients who were diagnosed with high-grade spinal cord astrocytoma were included. Clinical data consisting demographic, radiological, molecular features and treatment data were recorded. Univariate and multivariate Cox analysis were performed to investigate variables associated with survival outcome of histological high-grade spinal cord astrocytoma. Results: Median survival time was 21 months. Overall survival (OS) at 1 and 3 years was 65.7% and 40.7%, respectively. Sex, location, and tumor span did not present significant association with OS. Patients with H3K27M mutation showed significant shorter duration of symptom than patients with H3K27 wild-type. As respect to adjuvant treatment, adjuvant radiotherapy and chemotherapy were associated with favorable OS (both p = 0.01). Younger patients (age ≤ 18 years) had shorter OS (p = 0.008) than adult patients (age > 18 years). Of note, H3K27M mutation did not show significant impact on the survival outcome, regardless of histology grade 3 or grade 4 (p = 0.3). Conclusion: Histological high-grade spinal cord astrocytoma has dismal prognosis. Our study demonstrated that H3K27M mutation did not show significant impact on survival outcome of histological high-grade spinal cord astrocytoma.
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