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Lim, Hee-Joung,Seok, Ae Eun,Han, Jiyou,Lee, Jiyeong,Lee, Sungeun,Kang, Hee-Gyoo,Cha, Byung Heun,Yang, Yunseok The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.2
Objective: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. Methods: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. Results: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. Conclusion: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.
Comparison of Soyasapogenol A, B Concentrations in Soybean Seeds and Sprouts
Eun-Young Kang,Seung-Hyun Kim,Sun-Lim Kim,Su-Hyun Seo,Eun-Hye Kim,Hong-Keun Song,Joung-Kuk Ahn,Ill-Min Chung 韓國作物學會 2010 Korean journal of crop science Vol.55 No.2
Soybean seeds contain many biologically active secondary metabolites, such as proteins, saponins, isoflavones, phytic acids, trypsin inhibitors and phytosterols. Among them, saponins in soybeans have attracted considerable interest because of their health benefits. Soyasaponin A and B are the most abundant types of saponins found in soybeans along with soyasapogenol (aglycone), which is a precursor of soyasaponin. The main purpose of this experiment was to determine the concentration of soyasapogenol in soybean seeds and sprouts as a function of seed size, usage, seed coat color and seed cotyledon color. The 79 Korean soybean varieties were cultivated at Yesan of Chungnam in 2006 for the analysis of soyasapogenol using HPLC with Evaporative Light Scattering Detection (ELSD). The total average concentration of soyasapogenol was 1313.52~mug~;g-1 in soybean seeds and 1377.22~mug~;g-1 in soybean sprouts. Soybean sprouts were about 5% higher than soybean seeds in average total soyasapogenol concentration. In the process of sprouting, the average soyasapogenol A content decreased by approximately 1.6%, but soyasapogenol B and total soyasapogenol increased by 8.31% and 4.88%, based on the content of soybean seeds. When classified according to the size of seeds, the total soyasapogenol concentration of soybean seeds were not significantly different (p<0.05) On average, small soybean seeds were increased by as much as 103.14~mug~;g-1 in sprouting process. As a function of the use of the seeds, The total soyasapogenol in soybean seeds were significantly different (p<0.05). While, the soybean sprouts were not significant different (p<0.05). Altogether, sprout soybean seeds show the greatest change in content during the germination process. When seeds with different coat colors were compared, the total soyasapogenol concentration of soybean with yellow seed coats (1357.30~mu g~;g1 ) was slightly higher than that of soybean with black (1260.30~mug~;g-1 ) or brown (1263.62~mug~;g-1 ) seed coats. For the color of the cotyledon, the total soyasapogenol concentration was significantly increased in green cotyledon during the germination and seedling process. The results of this study suggest the functional characteristics of soybeans through quantitative analysis of soyasapogenol. In addition, the concentration of soyasapogenol exhibited a change during the germination process, which was evaluated by the nutritional value of the soybean sprouts.
( Joung Ah Park ),( Gi Dae Kim ),( Jung Hoon Cha ),( Hye Lim Kim ),( Eun Suk Choi ),( Eun Sun Jung ),( Seung Kew Yoon ),( Seh Hoon Oh ),( Si Hyun Bae ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.4
Mesenchymal stem cells (MSCs) derived from various human tissues, including amnion (AM) from the placenta and bone marrow (BM), have been known to differentiate into multi-lineages and mesodermal cell lines. Few comparative studies have reported their potential for hepatogenic differentiation using an in vitro culture system and it remains unclear if their therapeutic potential in an animal model also depends on this differentiation. The pur-pose of this study was to compare the hepatogenic differentiation capacity of AM- and BM-derived MSCs, and toevaluate their antifibrotic efficacy after direct transplantation into a bile duct-ligated rat model of liver fibrosis. The proliferation of AM-MSCs derived from placenta was higher than that of BM-MSCs. Differentiated hepatocyte-like cells changed in morphology, expressed hepatocyte-specific genes, and demonstrated functional activities. Differ-entiated AM- and BM-derived hepatocyte-like cells were directly transplanted into rat livers suffering from hepaticfibrosis due to bile duct ligation. Liver tissues were analyzed at one and two weeks post-transplantation. Albumin expression was increased in rats one week after transplanting AM- and BM-derived hepatocyte-like cells. And, col-lagen deposition decreased compared with the control group. These results contribute to our understanding of the antifibrotic effects of AM- and BM-derived hepatocyte-like cells on liver disease and their ability to undergo hepato-genic differentiation. AM- and BM-derived MSCs may be a source of cells for liver regeneration and may providea foundation for the development of novel cell therapies.
Fabrication and Characterization of High-activity Pt/C Electrocatalysts for Oxygen Reduction
Lim, Bo-Rami,Kim, Joung-Woon,Hwang, Seung-Jun,Yoo, Sung-Jong,Cho, Eun-Ae,Lim, Tae-Hoon,Kim, Soo-Kil Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.6
A 20 wt % Pt/C is fabricated and characterized for use as the cathode catalyst in a polymer electrolyte membrane fuel cell (PEMFC). By using the polyol method, the fabrication process is optimized by modifying the carbon addition sequence and precursor mixing conditions. The crystallographic structure, particle size, dispersion, and activity toward oxygen reduction of the as-prepared catalysts are compared with those of commercial Pt/C catalysts. The most effective catalyst is obtained by ultrasonic treatment of ethylene glycol-carbon mixture and immediate mixing of this mixture with a Pt precursor at the beginning of the synthesis. The catalyst exhibits very uniform particle size distribution without agglomeration. The mass activities of the as-prepared catalyst are 13.4 mA/$mg_{Pt}$ and 51.0 mA/$mg_{Pt}$ at 0.9 V and 0.85 V, respectively, which are about 1.7 times higher than those of commercial catalysts.
Awareness Survey on Community Water Fluoridation by Region
Joung Ye-Eun,Kim Min-Hee,Kim Hyo-Lim,Baek Ji-Ye,Jang Yun-Jeong,Choi Jae-Yi,오상환 한국치위생과학회 2022 치위생과학회지 Vol.22 No.4
Background: The purpose of this study is to investigate the pro-con of re-implementation by administrative areas and the difference in perception of community water fluoridation in implemented and non-implemented areas after the community water fluoridation in Korea was suspended. Through this, we intend to provide basic data that can help find ways to increase the support and interest of local residents.Methods: The 601 questionnaires collected through the survey and statistical analysis was conducted using SPSS Statistics 28.0. Results: As a result of analyzing the perception of the community water fluoridation according to the understanding of fluorine, the proportion of people who were not recognized by both fluorine and community water fluoridation was the highest (p<0.05). As a result of the analysis of the pro-con of re-implementation of community water fluoridation, the approval was high. Among those who responded in favor, the place of re-implementation showed that ‘implementation nationwide’ was high. As for the reason for favor, it was found that it was possible to prevent dental caries disease. The reason for the objection was the lack of knowledge about fluoride. Conclusion: The results of the survey for the pro-con of the re-implementation of community water fluoridation showed a higher degree of ‘agree’ and showed that people in the area where community water fluoridation was not implemented showed higher interest in oral health prevention and management. Through this, not only oral education, but also correct information on the implementation method of community water fluoridation, the benefits of community water fluoridation, and the facts that were misunderstood in the past, as well as oral education, can be provided to raise interest in community water fluoridation. It is thought that the expected effect of the re-implementation of community water fluoridation can be obtained if such activities are carried out.