부모의 코칭리더십이 고등학생 자녀의 그릿에 미치는 영향: 심리적 임파워먼트의 매개효과

조은별 ( Eun-byul Cho ),탁진국 ( Jin-kook Tak ) 한국청소년학회 2022 청소년학연구 Vol.29 No.11

The purpose of this study is to elucidate the causal mechanism that parental coaching leadership leads to children's grit through psychological empowerment for high school students, and the mediating effect of psychological empowerment in the relationship between parental coaching leadership and grit was investigated. For this purpose, parental coaching leadership, psychological empowerment, and grit were measured for 313 high school students across the country. For data analysis, mediating effects were verified using SPSS 25.0 SPSS Macro Process 4.1. As a result of the study, it was found that psychological empowerment had a mediating effect on the relationship between parental coaching leadership and grit. This study is the first study on parental coaching leadership, and it is meaningful in that it is the first study to reveal that coaching leadership mediates psychological empowerment and affects grit even in the relationship between parents and children, not at work. The results of this study suggest that education and experience should be provided to improve the psychological empowerment of high school children along with the development of programs to improve parents' coaching leadership and the spread of coaching culture in the home.

Anti-Apoptotic Pathway by Specific Wavelength of Irradiation in SNP-Induced SH-SY5Y Neuroblastoma Cell

Won Bong Lim,Sung Ga Lee,Eun Byul Kook,ln Ae Kim,Min A Jung,Ok Joon Kim,Hong Ran Choi 대한구강악안면병리학회 2007 대한구강악안면병리학회지 Vol.31 No.2

The nitric oxide(NO) is a major factor contri buting to t he loss of neurons in ischemic st roke. demyelina t ing diseases, and other neurodegenerative di sorders . But it is known that NO is not function ing as a direct neurotoxin. NO combined with superoxide(02-) by the diffusion-contl'O ll ed reaction, formed a peroxin it ri te anion (ONOO-)‘ which this s pecies has been shown to contribute to oxidative s igna ling and damage. ONOO stimuJates apoptosis in many cell types. whether ONOO acts direct ly as an ox idant 0 1' the induction of apoptosis is because of the radicals derived from ONOO- decompositi on . But. the mecha ni sm by which ONOO- induces apoptosis is un clear although subsequent forrnation 0 1' reactive oxygen s pecies(ROS) has been suggested in a few reports The aim of this study is to investigate the a nti -apoptotic pathway by inhibi tion 0 1' ONOO synthesis t hrough scavenging of ROS us ing s pecific wavelength 0 1' light irradiation . The present study investi gated the a nti -apop totic effect of the specific wavelength 0 1' irradi ation in Sodium Nitroprusside(SNP) t reated SJ-I-SY5Y ceJls, by MTT, DNA fragmentation, and flow cytometric assay and th rough western blot and caspase-3, -9 activity assay for confirmation of caspase pathway. Also. NO reJease and ROS leveJ was measured in order to observe the changes of NO involved in radical by Griess reaction analysis and DCF'-DH. Results showed that the cell viability were r educed by about 50% of control group by SNP treatment, but re covered to about 80% by 590nm irradiation . The apoptotic cells were observed by flow cytomet ry and DNA fra g mentation assay in SNP-treated group‘ but 590nm irradiation led apoptotic feature to be reduced . Released NO a nd ROS level were increased after SNP treatment but ROS level was dec reased in 590nm irradi at ion - treated group, in spite of high NO concentration fo llowing SNP treatment Also. SNP t reatment led cytochrom C release but 590nm irraidiation inhibit it, hence the expression 0 1' caspase-3 and -9 was dec reased sign ificantly‘ These results showed that 590nm irradiation protect neuronal death thl'Ough bl ocking of NO-induced mi tochondri al apoptotic pathway. Also, it suggests that specific wavelength of irradi ation was used for prevent ion from neurodegenerative disordcr progression

Photodynamic Therapy of Oral Squamous Cell Carcinoma Using Reactive Oxygen Species-responsive Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-conjugated Chitosan Conjugates

Chang-Young KIM,Chang-Min LEE,Eun-Byul KOOK,Byung-Hoon KIM,Young-Il JEONG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

Photosensitizers for photodynamic therapy (PDT) of cancer generate reactive oxygen species (ROS) under light irradiation and, therefore, they have minimal side-effects in the normal cells or tissues [1]. However, photosensitizers are dispersed freely in the whole body by systemic administration and then causes phototoxicity problem in the skin. This issue requires that patients protect from sun-light for a long time. For this study, chitosan was conjugated with phenyl boronic acid pinacol ester (PBAP) and chlorin e6 (Ce6)-incorporated nanophotosensitizers were fabricated for ROS-sensitive delivery of photosensitizers against cancer. Ce6-incorporated chitosan-PBAP nanophotosensitizers (Ce6-ChitoPBAP) were evaluated with SCC-15 oral squamous carcinoma cells (OSCC). Ce6-incorporated nanophotosensitizers showed spherical shapes in TEM observation with less than 300 nm diameter. Ce6 was released from nanophotosensitizers with ROS-sensitive behavior, i.e. Ce6 release rate was faster in the presence of hydrogen peroxide. Ce6-incorporated nanophotosensitizers showed higher phototoxicity against SCC-15 OSCC compared to Ce6 itself while they have negligible toxicity against cancer cells and normal cells in the absence of light. In conclusion, Ce6-incorporated nanophotosensitizers can be considered as a suitable option for PDT of OSCC.

사람 치은모세포의 세포주기에 미치는 light-emitting diode 조사 효과

Jung Hye Kim,Youn Young Jang,Kwon Jung,Mi Kyeong Ko,Mi Sun Jang,Eun Byul Kook,Min A Chong,Won Bong Lim,Chae Kwang Lim,Ok Joon Kim,Hong Ran Choi 대한구강악안면병리학회 2006 대한구강악안면병리학회지 Vol.30 No.2

It has been reported that light-emitting diodes(LED) can be used in the treatment of oral diseases. Although bio-stimulatory effects of LED irradiation such as promotes stimulation of wound healing have been well known, there are few reports about molecular mechanism associated with cell cycle by LED irradiation. The purpose of present study was to examine the molecular event in cell cycle of LED irradiation on primary human gingival fibroblast(hGF) in vitro. The source of light for irradiation was a continuous-wave LED emitting at a wavelength of 635nm, and manufactured that energy density was 5mW/cm2 on sample surface. The hGF were irradiated for 1 hour at 37℃ in 5% CO2 humidified chamber. Experimental samples were acquired at 0 (right after irradiation), 8 and 24 hour after irradiation. To investigate the molecular mechanisms associated with cell cycle, growth phase was determined by flow cytometry and mRNA expression of cyclin A, cyclin B, cyclin D1, cyclin E, cdc2, PCNA, p18, p27, p21, and p53 were determined by real time RT-PCR. Flow cytometric analysis demonstrated the percentage of cells in the G1 and S phase were decreased, but the G2 phase increased, which showed cells irradiated by LED were transitioned from S to G2 phase. For mRNA expression, cyclin B, cdc2, PCNA and p53 were increased at 0 hour after irradiation, and most of cell cycle molecules were increased at 8 hour after irradiation. At 24 hour after irradiation, cyclin A, cyclin E, PCNA and p18 were increased. Taken together, LED irradiation induced proliferation of hGF cells through transition from S to G2 phase.

LED Irradiation Decreases CoCb-induced Apoptosis In Human SH-SY5Y Neuroblastoma Cells : Signaling Mechanisms and Therapeutic Opportu

Jae Hyuck Yang,Youn Young Jang,Kwon Jung,Mi Kyeong Ko,Mi Sun Jang,Eun Byul Kook,Chae Kwang Lim,Won Bong Lim,Jin Soo Park,Ok Joon Kim,Hong Ran Choi 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5

The purpose 01' pl'esent study was to examine the molecular events in apoptosis by CoCl2, mimicking hypoxic cond ition and recovering effects by LED ir l'adiation on Human SH-SY5Y neuroblastoma cells The SOUl'ce 0 1' light for ir l'adiation was a continuous-wave LED emitting at a wavelenl양h of 590 nm, and manufactured that ene rgy density was 5 mW!cm2 on sample surface, After ir l'adiation, cell viabi lity was measured with BrdU , cell morphol ogy was examined with Diff- Quik staining, cell signaling was monitored with various apoptosis-related molecules using RNase Pl'otection Assay(RPA) , W11en treated with CoC12, apoptotic induction was found in the SH-SY5Y cells in a concentration-dependent and time-dependent manner , Diff-Quik s taining was revealed that DNA fragmentation re presented apoptosis was examined in CoC12-tl'eated group, Moreover, RPA assay of SH-SY5Y cclls lIs ing val'iolls apoptosis-related molecllles showed that the apoptotic cell population was mcreased J-loweve. there was sorne signifïcant change in LED irradiatied cells aftel' treatement of CoC12 The main mechanism for Lhese a poptosis appearecl to be mito c hondriεt - m ecliated pathway, such as cytochrome- c‘ caspase-9, caspase-3, pro-apototic protein ßax, anti-apototic protein Bcl-2, and death receptor• mediated pathway, such as Fas, cas pase- 8, a ncl TNFRl These results demonstrate that CoCI2 induce apoptosis in SH-SY5Y via different dual apop tosis pathway through death receptor pathway as well as mitochondria- dependent pathway and LED irradiation can recl llces the CoCl2-induced apoptosis by blocking their internal signaling pathway

Expression of Bcl-2 Family in 4-Nitroquinoline 1-Oxide-Induced Tongue Carcinogenesis of the Rat

최재욱,정성수,이금숙,김병국,김재형,국은별,장미선,고미경,정권,최홍란,김옥준,Choi, Jae-Wook,Chung, Sung-Su,Lee, Geum-Sug,Kim, Byung-Gook,Kim, Jae-Hyeong,Kook, Eun-Byul,Jang, Mi-Sun,Ko, Mi-Kyeong,Jung, Kwon,Choi, Hong-Ran,Kim, Ok-Joon Korean Academy of Orofacial Pain and Oral Medicine 2005 Journal of Oral Medicine and Pain Vol.30 No.3

The number of patients with tongue carcinoma is increasing rapidly among young individuals in many parts of the world. Oral carcinoma progresses from hyperplastic lesion through dysplasia to invasive carcinoma and the concept of "field cancerization" with molecular alteration has been suggested for oral cavity carcinogenesis. Significant improvement in treatment and prognosis will depend on more detailed understanding of the multi-step process leading to cancer development. To induce tongue carcinoma in rat by 4-NQO, each drinking water was made to 10 ppm, 25 ppm, 50 ppm and control (only D.W. without 4-NQO). Specimens were classified into 4 groups such as control, I (mild & moderate dysplasia), II (severe dysplasia and carcinoma in situ), III (carcinoma). The mRNA expressions of Bcl-2 family were evaluated by RT-PCR technique. For anti-apoptotic Bcl-2 family, mRNA expression of Bcl-w was down-regulated in all stages of tongue carcinogenesis model. However, mRNA expression of Bcl-2 was up-regulated. For pro-apoptotic Bcl-2 family, all members were down-regulated in all stages of tongue carcinogenesis model except for Bad mRNA in group III. In terms of BH3 only protein, mRNA expressions of Bok and Mcl-1 were down regulated in all stages of specimen, but Bmf in group II and BBC3 in group III were up-regulated. Our current findings demonstrated the involvements of mRNA expression of Bcl-2 family in multi-step tongue carcinogensis. This highlights the necessity for continued efforts to discover suitable biomakers (Bcl-2 family) for early diagnosis of the disease, and to understand its pathogenesis as a first step in improving methods of treatment. The discovery of these potential biomarkers and molecular targets for cancer diagnostics and therapeutics has the potential to significantly change the clinical approach and outcome of the disease.