유아 멀티미디어 교육 활성화를 위한 방향

서현아,고은미 경성대학교 2000 論文集 Vol.21 No.2

The Education of Multimedia in the kindergarten become more and more important to young children these days. However most educators didn't integrate multimedia activities to their other parts of the classroom. The purpose of this study is to recognize the importance of multimedia education for young children and promote teacher's positive role. Accordingly, this study examined computer activity, software, use of internet and the factors the influence teacher to integrate computer technology into the teaching and learning process. The results of this study are as follows, 1) Multimedia should be understood by the concept of multimedia computer and interacting environment. 2) The important thing in computer activity is not controversy about computer effects but how we connect computer activity into children's life well. 3) Developmentally appropriate software is essential part of successful computer activities. 4) Developmentally appropriate internet program may serve as an interesting, creative platform in the acquisition of general skills. 5) External factors and intern릴 factors should be considered together for early multimedia education and integrate multimedia activity to children's environment.

Effect of High Fructose Corn Syrup (HFCS) Intake on the Female Reproductive Organs and Lipid Accumulation in Adult Rats

Eun-Ah Ko,Hye-Ri Kim,Yong-Bin Kim,Hee-Su Kim,Sung-Ho Lee 한국발생생물학회 2017 발생과 생식 Vol.21 No.2

High-fructose corn syrup (HFCS) is widely used as sweetener, and its overconsumption is become a major health problem. In the present study, we used adult female rats and applied a 28 days HFCS feeding model to monitor the estrous cycle and changes in tissue weights and histology. Adult female rats were divided into three groups. Animals were fed with ad libitum normal chow and (1) 24 hours tap water (Control group), (2) 12 hours HFCS access during dark period and 12 hours tap water (12H group), and (3) 24 hours HFCS only access (24H group). Total exposure period was 28 days. There is no significant change in body weight between control and HFCS-fed animals. Both absolute and relative weights of ovary in 24H animals were significantly heavier than those in control or 12H animals. The absolute and relative weights of the kidney and liver in 24H groups were significantly heavier than those in control or 12H animals. The estrous cycles of the 24H animals were significantly longer. Histological analyses revealed that 24H ovaries were relatively bigger and possessed more corpus lutea than control ovaries. Uterine sections of 12H and 24H animals showed a well-developed stratum vasculare between inner and outer myometrial layers. The number of endometrial glands were decreased in 12H uteri, and recovered in 24H uteri compared to control. Numbers of convoluted tubule in distal region increased in 12H and 24H kidney samples. Liver specimens of 12H and 24H showed the increased number of fat containing vacuoles. In conclusion, our study demonstrated that HFCS treatment for 28 days could induce (1) changes in length of estrous cycle with extended estrous and diestrous stages, (2) altered ovarian and uterine histology, and (3) liver and renal lipid accumulation. These findings reveal the adverse effects of HFCS drinking on the reproductive function and lipid metabolism of female rats.

Construction and Expression of a Bifunctional Enzyme Dextranase and Maltohexaose-producing Amylase activities in Escherichia coli

Eun Ah Ko,Jin Kang,Yun Lee,Doman Kim 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.10

Effect of High Fructose Corn Syrup (HFCS) Intake on the Female Reproductive Organs and Lipid Accumulation in Adult Rats

Ko, Eun-Ah,Kim, Hye-Ri,Kim, Yong-Bin,Kim, Hee-Su,Lee, Sung-Ho The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.2

High-fructose corn syrup (HFCS) is widely used as sweetener, and its overconsumption is become a major health problem. In the present study, we used adult female rats and applied a 28 days HFCS feeding model to monitor the estrous cycle and changes in tissue weights and histology. Adult female rats were divided into three groups. Animals were fed with ad libitum normal chow and (1) 24 hours tap water (Control group), (2) 12 hours HFCS access during dark period and 12 hours tap water (12H group), and (3) 24 hours HFCS only access (24H group). Total exposure period was 28 days. There is no significant change in body weight between control and HFCS-fed animals. Both absolute and relative weights of ovary in 24H animals were significantly heavier than those in control or 12H animals. The absolute and relative weights of the kidney and liver in 24H groups were significantly heavier than those in control or 12H animals. The estrous cycles of the 24H animals were significantly longer. Histological analyses revealed that 24H ovaries were relatively bigger and possessed more corpus lutea than control ovaries. Uterine sections of 12H and 24H animals showed a well-developed stratum vasculare between inner and outer myometrial layers. The number of endometrial glands were decreased in 12H uteri, and recovered in 24H uteri compared to control. Numbers of convoluted tubule in distal region increased in 12H and 24H kidney samples. Liver specimens of 12H and 24H showed the increased number of fat containing vacuoles. In conclusion, our study demonstrated that HFCS treatment for 28 days could induce (1) changes in length of estrous cycle with extended estrous and diestrous stages, (2) altered ovarian and uterine histology, and (3) liver and renal lipid accumulation. These findings reveal the adverse effects of HFCS drinking on the reproductive function and lipid metabolism of female rats.

A Study on the Movement Creation Process of the Work 〈Invisible〉 that is based on the Visualization of Music

Ko Eun-jeong,Hwang Eun-ah,Kim Hye-jin 한국콘텐츠학회 2017 한국콘텐츠학회 ICCC 논문집 Vol.2017 No.12

실리카-커플링제-고무의 화학 결합 형성과 특성 분석

고은아 ( Eun Ah Ko ),최성신 ( Sung Seen Choi ) 한국고무학회 2014 엘라스토머 및 콤포지트 Vol.49 No.3

용매를 사용하지 않고 실리카와 실란커플링제의 반응을 투과 방식 휴리에 변환 적외선 분광법(FTIR)과 열중량 분석법(TGA)을 이용하여 조사하였다. 실란커플링제로 bis[3-(triethoxysilylpropyl) tetrasulfide] (TESPT)를 사용하였다. 미반응 TESPT를 제거한 후, FTIR로 화학 결합 형성을 분석하였고 TGA로 반응한 TESPT 함량을 결정하였다. 커플링제의 함량이 증가할수록 실리카에 결합한 커플링제의 양이 증가하였으나, 커플링제를 과량으로 첨가하면 커플링제 간의 축합반응에 의해 올리고머를 형성하였다. 실리카와 커플링제 그리고 고무의 결합을 확인하기 위하여 개질 실리카와 저분자량 액상 BR을 반응시켜 실리카-커플링제-BR 모델 복합체를 제조하여 화학 결합 형성을 조사하였다. 미반응 고무는 용매를 사용하여 제거하였고 FTIR과 TGA로 분석하였다. BR은 개질 실리카의 커플링제와 반응하여 화학 결합을 형성하였다. 실리카-커플링제-BR의 화학 결합 형성으로 인해 실리카 표면의 극성은 크게 낮아졌고 실리카 입자 크기는 커지는 효과를 보였다. Reaction between silica and silane coupling agent without solvent was investigated using transmission mode Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). Bis[3-(triethoxysilylpropyl) tetrasulfide] (TESPT) was used as a silane coupling agent. After removing the unreacted TESPT, formation of chemical bonds was analyzed using FTIR and content of reacted TESPT was determined using TGA. Content of the coupling agent bonded to silica increased with increase in the coupling agent content, but the oligomers were formed by condensation reaction between coupling agents when the coupling agent was used to excess. In order to identify bonds formed among silica, coupling agent, and rubber, a silica-coupling agent-BR model composite was prepared by reaction of the modified silica with liquid BR of low molecular weight and chemical bond formation of silica-coupling agent-BR was investigated. Unreacted rubber was removed with solvent and analysis was performed using FTIR and TGA. BR was reacted with the coupling agent of the modified silica to form chemical bonds. Polarity of silica surface was strikingly reduced and particle size of silica was increased by chemical bond formation of silica-coupling agent-BR.

유자씨 오일의 항산화, 항염, 항알러지 효과 및 인체 피부보호 효과에 대한 연구

고은아 ( Eun Ah Ko ),남승희 ( Seung-hee Nam ),정하나 ( Hana Jeong ),김보윤 ( Bo Yun Kim ),곽상화 ( Sang Hwa Kwak ),김선영 ( Sunyoung Kim ),홍인기 ( In Ki Hong ),강학희 ( Hakhee Kang ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.3

본 연구에서는 유자 완숙과의 중량비 13%를 차지하지만 대부분 폐기 처분되어 활용이 되지 않는 유자씨앗의 활용도를 높이기 위하여, 냉압착 방법으로 추출한 유자씨 오일의 피부미용 효능을 연구하였다. 유자씨오일은 주로 oleic acid와 linoleic acid로 이루어진 불포화 지방산을 약 74% 함유 하는 것으로 확인되었으며, 주로 유자 과피에 포함되는 limonene은 약 0.0187%로 매우 낮은 함량을 포함하였다. 유자씨 오일의 DPPH radical 소거 활성능력을 평가한 결과, 5% 농도에서 26%의 DPPH 라디칼 소거능력을 확인하였다. 항염 효능을 확인하기 위해 유자씨오일에 대한 RAW 264.7 세포 독성 시험 및 NO 생성을 시험한 결과, 세포 독성을 나타내지 않는 0.05% 농도에서 NO 생성을 53% 억제하였다. 그리고 항알러지 효능확인을 위한 RBL-2H3 세포 독성 및 β-hexosaminidase 방출 억제 효능 시험에서도 세포 독성을 나타내지 않는 0.05% 농도에서 β-hexosaminidas 방출을 26% 억제함을 확인하였다. 마지막으로 유자씨 오일을 5% 함유한 O/W 에멀젼의 인체적용 시험 결과에서는 caprylic/capric triglyceride를 동량 함유하는 대조 에멀젼에 비하여 높은 피부 보습효능을 나타내었다. 따라서 유자씨 오일은 우수한 스킨케어 소재로 활용 될 수 있을 것으로 판단된다. In this study, in order to increase the utilization of Citrus junos seeds, which account for 13% of the weight ratio of Citrus junos ripened fruit, but are mostly discarded and not utilized, the efficacy of skin beauty of Citrus junos seed oil extracted by cold pressing was studied. Citrus junos seed oil was found to contain approximately 74% of unsaturated fatty acids consisting mainly of oleic acid and linoleic acid, and limonene, which is mainly contained in Citrus junos peel, contained a very low content of about 0.0187%. As a result of evaluating the DPPH radical scavenging activity of Citrus junos seed oil, 26% of DPPH radical scavenging ability was confirmed at 5% concentration of Citrus junos seed oil. To confirm the anti-inflammatory effect, as a result of testing RAW 264.7 cytotoxicity test and NO production for Citrus junos seed oil, NO production was suppressed by 53% at a concentration of 0.05% that does not show cytotoxicity. In addition, in the RBL-2H3 cytotoxicity and β-hexosaminidase release inhibitory efficacy test for anti-allergic efficacy confirmation, it was confirmed that β-hexosaminidas release was suppressed by 26% at a concentration of 0.05% that did not show cytotoxicity. Lastly, in the human skin application test result of O/W emulsion containing 5% of Citrus junos seed oil, it showed higher skin moisturizing effect than the control emulsion containing the same amount of caprylic/capric triglyceride. Therefore, it is thought that Citrus junos seed oil might be used as a excellent skin care material.

노인 안심주택에 관한연구* - 주거안전사고 예방을 중심으로 -

고영아(KO, Young Ah)홍은표(HONG, Eun-Pyo) 대한부동산학회 2019 大韓不動産學會誌 Vol.37 No.2

한국소비자원 보고서(2017)에 따르면 우리나라 내 노인안전사고 발생지역의 67.2%는 가정이고, 가정 내에서 안전사고가 발생하는 장소는 욕실, 주방, 거실, 침실, 계단 등의 순인 것으로 나타났다. 따라서 노령화가 심화되어 가는 우리나라의 현실에서 노인을 위한 안심주택의 주거모델에 관한 포괄적 연구가 필요하다. 연구목적은 독거노인들의 주거 및 주거안전사고실태를 분석하고, 주거안전사고 예방을 위한 노인 안심주택 에 대한 주거모델의 기초자료를 제시하는 것이다. 연구를 위해 국토교통부의 ‘주거실태조사(2017)’, 보건복지부의 ‘노인실태조사(2017)’ 및 한국보건사회연구원의 ‘노 인의 지역사회 계속 거주를 위한 장기요양제도 개편 방안(이윤경, 2017)’을 활용한다. 또한 한국소비자원의 ‘고령자 안전사고 심층 분석(2017)’ 및 통계청의 ‘가계 동향조사(2016)’도 분석에 활용한다. 연구결과는 크게 5가지로 요약할 수 있다. 첫째, 주거 공간 내 구조 및 공적 공간 배치의 개선. 둘째, ICT를 활용한 노인주거시스템 구축. 셋째, 건강 수준과 가족구조에 따른 주거의 다양성 확보. 넷째, 세대 공존형 코하우징 모델 개발. 다섯째, 노인층에 대한 주택개조 비용 지원확대가 필요한 것으로 나타났다. According to the Korea Consumer Reports (2017), 67.2 percent of the elderly safety accidents in Korea are at home, while the number of safety accidents in the home is in the order of bathrooms, kitchens, living rooms, bedrooms and staircases. Therefore, a comprehensive study on the residential model of safe house for the elderly is needed in the face of the deepening aging population in Korea. The purpose of the research is to analyze the actual conditions of residential and residential safety accidents of the elderly living alone and present basic data on the old safe house for prevention of housing safety accidents. For the research, the ministry will utilize the Land, Infrastructure and Transport Ministry s Residence Survey (2017), the Ministry of Health and Welfare s A Survey on the Actual Conditions of the Elderly (2017) and the Korea Institute for Health and Social Affairs Plans to Reform the Long-Term Care System for Continuing Elderly Community (Lee Yoon-kyung, 2017). Further, it will use the Korea Consumer Agency s In-depth analysis of safety accidents (2017) and the National Statistical Office s Household Trend Survey (2016) in its analysis. The findings can be summarized into five main categories. First, improvement in structure and public space layout in residential space. Second, the establishment of a housing system for the elderly using ICT. Third, securing diversity of residence according to health level and family structure. Fourth, development of generation-friendly co-housing model. Fifth, it was found that more support for housing refurbishment was needed for the elderly.

LC-MS/MS를 이용한 축산물 중 Spinetoram 공정시험법 개발 및 검증

고아영(Ah-Young Ko),김희정(Heejung Kim),도정아(Jung Ah Do),장진(Jin Jang),이은향(Eun Hyang Lee),주윤지(Yun Ji Ju),김지영(Ji Young Kim),장문익(Moon-Ik Chang),이규식(Gyu-Seek Rhee) 한국분석과학회 2016 분석과학 Vol.29 No.2

LC-MS/MS를 이용한 농산물 중 Isoxaflutole과 대사산물(Diketonitrile)의 동시시험법 개발

고아영(Ah-Young Ko),김희정(Heejung Kim),도정아(Jung Ah Do),장진(Jin Jang),이은향(Eun-Hyang Lee),주윤지(Yunji Ju),김지영(Ji Young Kim),장문익(Moon-Ik Chang),이규식(Gyu-Seek Rhee) 한국농약과학회 2016 농약과학회지 Vol.20 No.2

A simultaneous analytical method was developed for the determination of isoxaflutole and metabolite (diketonitrile) in agricultural commodities. Samples were extracted with 0.1% acetic acid in water/acetonitrile (2/8, v/v) and partitioned with dichloromethane to remove the interference obtained from sample extracts, adjusting pH to 2 by 1 N hydrochloric acid. The analytes were quantified and confirmed via liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive-ion mode using multiple reaction monitoring (MRM). Matrix matched calibration curves were linear over the calibration ranges (0.02-2.0 ㎍/mL) for all the analytes into blank extract with r2 > 0.997. For validation purposes, recovery studies were carried out at three different concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates at each level. The recoveries were ranged between 72.9 to 107.3%, with relative standard deviations (RSDs) less than 10% for all analytes. All values were consistent with the criteria ranges requested in the Codex guideline (CAC/GL40, 2003). Furthermore, inter-laboratory study was conducted to validate the method. The proposed analytical method was accurate, effective, and sensitive for isoxaflutole and diketonitrile determination in agricultural commodities.