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Ba, Nguyen Xuan,Van Huu, Nguyen,Ngoan, Le Duc,Leddin, Clare M.,Doyle, Peter T. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.12
Two experiments were conducted in central Vietnam to test the hypothesis that supplementation with a concentrate, comprising rice bran (45% fresh basis), maize (49%), fish meal (3%), urea (2%) and salt (1%), up to 2% of live weight (LW)/d (dry matter (DM) basis) would linearly increase digestible organic matter intake and LW gain of yellow cattle. In both experiments, there were five treatments, namely a basal diet of fresh grass fed at 1.25% of LW (experiment 1, elephant grass, Pennisetum purpureum; experiment 2, native grass) and rice straw (Oryza sativa) fed ad libitum or this diet supplemented with concentrate at about 0.3, 0.7, 1.3 or 2.0% LW. There were 4 male growing cattle per treatment in experiment 1 and 3 in experiment 2. Diets were fed for 44 (experiment 1) or 49 (experiment 2) days, with feed intake recorded daily, LW measured about weekly and digestibility measurements made over 7 days commencing on day 24 (experiment 1) or day 10 (experiment 2). The elephant grass and native grass had neutral detergent fibre (NDF) concentrations of 82 and 73% DM, and nitrogen concentrations of 1.3 and 1.8% DM, respectively. The rice straw used had a NDF concentration of 79-84% DM and nitrogen concentration of 0.8% DM. The concentrate had NDF and nitrogen concentrations of 33 and 2.8% DM. In both experiments, DM intake increased (p<0.001) linearly as the amount of concentrate consumed increased. Rice straw intake declined (p<0.001) (experiment 1: 1.24 to 0.48 kg DM/d; experiment 2: 0.95 to 0.50 kg DM/d) as concentrate intake increased. Grass intake was not significantly affected by concentrate intake in either experiment. The lowest amount of concentrate supplement increased forage intake, after which substitution rate increased as the amount of concentrate consumed increased. However, substitution rates at the highest amount of concentrate consumed were modest at 0.3 to 0.5 kg DM reduction in forage intake/kg DM supplement consumed. In both experiments, digestible organic matter intake increased linearly (p<0.001) (experiment 1: 1.16 to 2.38 kg/d; experiment 2: 1.30 to 2.49 kg/d) as the amount of supplement consumed increased, as did LW gain (experiment 1: 0.15 to 0.81 kg/d; experiment 2: 0.15 to 0.77 kg/d). This was associated with significant (p<0.01) linear increases in organic matter intake and apparent organic matter digestibility. Neutral detergent fibre digestibility declined as concentrate intake increased, but the effect was not significant (p = 0.051) in experiment 2. These results are discussed in relation to existing literature and potential to improve the profitability of cattle fattening in central Vietnam.
L. Nguyen-Ngoc,H. Tran-Ngoc,T. Bui-Tien,A. Mai-Duc,M. Abdel Wahab,Huan X. Nguyen,G. De Roeck 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.1
In this paper, a novel approach to damage identification in structures using Particle Swarm Optimization (PSO) combined with Artificial neural network (ANN) is proposed. With recent substantial advances, ANN has been extensively utilized in a wide variety of fields. However, because of the application of backpropagation algorithms based on gradient descent techniques, ANN may be trapped in local minima when seeking the best solution. This may reduce the accuracy of ANN. Therefore, we propose employing an evolutionary algorithm, namely PSO to deal with the local minimum problems of ANN. PSO is employed to improve the training parameters of ANN consisting of weight and bias ratios by reducing the deviation between calculated and desired results. These training parameters are then used to train the network. Since PSO applies global search techniques to look for the best solution, it can assist the network in avoiding local minima by looking for a beneficial starting point. In order to assess the effectiveness of the proposed approach, both numerical and experimental models with different damage scenarios are employed. The results show that ANN -PSO not only significantly reduces computational time compared to PSO but also possibly identifies damages in the considered structures more accurately than ANN and PSO separately.
Huyen T. M. Pham,Duc Long Nguyen,Hyo‑Sop Kim,Yang Eun Kyeong,김재호,윤현철,박현지 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) gain attention as a potent cell source in regenerative medicine and drug discovery. With the necessity of the demands for experimental models to create a more physiologically relevant model of the heart in vitro we herein investigate a 3D culturing platform and a method for assessing rhythm in hiPSC-CMs. Methods The 3D cell culture PAMCELL™ plate is designed to enable cells to attach exclusively to adhesive patterned areas. These cell adhesive zones, named as micro-patterned pads, feature micron silica beads that are surfacemodified with the well-known arginyl-glycyl-aspartic acid (RGD) peptide. RGD binding to the surface of hiPSC-CMs facilitates cell–cell attachment and the formation of uniform-size spheroids, which is controlled by the diameter of the micro-patterned pads. The assessment and evaluation of 3D hiPSC-CMs beating pattern are carried out using reflective properties of retroreflective Janus micro-particle (RJP). These RJPs are modified with an antibody targeting the gap junction protein found on the surface of hiPSC-CM spheroids. The signal assessment system comprises a camera attached to an optical microscope and a white light source. Results The 3D PAMCELL™ R100 culture plate efficiently generate approximately 350 uniform-sized hiPSC-CM spheroids in each well of a 96-well plate and supported a 20-day culture. Analysis of genes and protein expression levels reveal that iPSC-CM spheroids grown on PAMCELL™ R100 retain cardiac stem cell characteristics and functions, outperforming traditional 2D culture platform. Additionally, the RJPs enable monitoring and evaluation of in vitro beating properties of cardiomyocytes without using complex monitoring setup. The system demonstrates its capability to identify alteration in the rhythmic activity of cardiac cells when exposed to ion channel blockers, nifedipine and E4031.
Ba, Nguyen Xuan,Van, Nguyen Huu,Ngoan, Le Duc,Leddin, Clare M.,Doyle, Peter T. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.8
An experiment was conducted in Vietnam to test the hypothesis that supplementation with cassava powder up to 2% of live weight (LW)/d (DM basis) would linearly increase digestible organic matter intake and LW gain of Laisind cattle. There were five treatments: a basal diet of elephant grass fed at 1.25% of LW and rice straw fed ad libitum or this diet supplemented with cassava powder, containing 2% urea, at about 0.3, 0.7, 1.3 or 2.0% LW. The cattle fed cassava powder at about 2.0% LW did not consume all of the supplement, with actual intake similar to the 1.3% LW treatment. Organic matter, digestible organic matter and digestible energy intakes increased (p<0.001) curvilinearly with increased consumption of cassava powder. Rice straw intake declined curvilinearly with increasing intake of cassava powder (p<0.001), and there was a small linear decline (p = 0.01) in grass intake. The substitution rate of cassava powder for forage was between 0.5 and 0.7 kg DM reduction in forage intake per kg DM supplement consumed, with no difference between treatments. Apparent digestibility of organic matter increased (p<0.001) in a curvilinear manner, while digestibility of neutral detergent fibre declined (p<0.001) in a curvilinear manner with increased consumption of cassava powder. Live weight gain increased (p<0.01) linearly with increased consumption of supplement. It was concluded that the amount of cassava powder fed should be limited to between 0.7 and 1.0% LW.
호앙구엔 ( Dung H. Nguyen ),민은엔 ( Duc T. M. Nguyen ),라련화 ( Lyun Hwa La ),이향복 ( Hyang Bok Lee ),신정현 ( Jung Hyun Shin ),김은기 ( Eun Ki Kim ) 대한화장품학회 2008 대한화장품학회지 Vol.34 No.3
미백제를 선발하기 위해 주로 사용하는 현재의 방법은 in vitro 타이로시네이즈 활성 및 항산화능을 측정하는 것이다. 이 결과에 기초하여 다음 단계인 멜라노사이트에서의 멜라닌 생성량을 측정한다. 세포 내의 멜라닌 생성량 측정법은 시간, 인력 및 숙련도가 요구된다. 따라서 초기 선발 방법의 신뢰성이 중요하다. 200개 중국시료 중 측정범위 내에서 세포독성이 없는 34개를 대상으로 세포 내 멜라닌량, 타이로시네이즈 활성, 항산화능의 상관관계를 조사하였다. 조사결과 직선의 상관관계를 확인할 수 없었다. 이 결과는 현재 선발방법의 한계 및 새로운 방법이 필요함을 보여주었다. Initial screening assay for depigmenting agents includes in vitro mushroom tyrosinase assay and antioxidant assay. Based on this screening result, melanin synthesis in melanocyte, in screened samples, is further measured. Measuring cellular melanin needs time, human resource, and skills. Therefore initial screening method should be reliable. We examined 34 Chinese herbs, correlated the screening assay methods with cellular melanin. No reliable relationship was observed between factors, indicating the limitation in the use of these assays, probably due to the complexicity of melanogenesis.