Transcriptome analysis of flower colour reveals the correlation between SNP and differential expression genes in Phalaenopsis

Ding Yu,Wang Ma-Yin,Yang Ding-Hai,Hao Dai-Cheng,Li Wei-Shi,Ling Peng,Xie Shang-Qian 한국유전학회 2023 Genes & Genomics Vol.45 No.12

Background Phalaenopsis is an important ornamental plant that has great economic value in the world flower market as one of the most popular flower resources. Objective To investigate the flower colour formation of Phalaenopsis at the transcription level, the genes involved in flower color formation were identified from RNA-seq in this study. Methods In this study, white and purple petals of Phalaenopsis were collected and analyzed to obtained (1) differential expression genes (DEGs) between white and purple flower color and (2) the association between single nucleotide polymorphisms (SNP) mutations and DEGs at the transcriptome level. Results The results indicated that a total of 1,175 DEGs were identified, and 718 and 457 of them were up- and down-regulated genes, respectively. Gene Ontology and pathway enrichment showed that the biosynthesis of the secondary metabolites pathway was key to color formation, and the expression of 12 crucial genes (C4H, CCoAOMT, F3’H, UA3’5’GT, PAL, 4CL, CCR, CAD, CALDH, bglx, SGTase, and E1.11.17) that are involved in the regulation of flower color in Phalaenopsis. Conclusion This study reported the association between the SNP mutations and DEGs for color formation at RNA level, and provides a new insight to further investigate the gene expression and its relationship with genetic variants from RNA-seq data in other species.

RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer

Ding Shi,Jinjin Liu 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.10

Purpose: Information on the possible role of the ribosomal protein S15a (RPS15a) in gastric cancer is scarce. The aim of this studywas to evaluate the impact of RPS15a gene expression on the growth and cell cycle of gastric cancer cells in vitro and in vivo. Materials and Methods: RPS15a mRNA expression was examined in cancer tissues and their corresponding adjacent normal tissuesof 40 gastric adenocarcinoma patients. Next, RPS15a was knocked down using a lentivirus-mediated RNA interference (shorthairpin RNA) system in the gastric cancer cell line BGC823. The effect of RPS15a knockdown was examined using CCK-8 assay,cell scratch test, colony formation assay, and flow cytometry. Finally, in nude mice, a tumorigenicity test was performed, and thetumor volume and weight were measured. Results: RPS15a expression in tumor tissue was significantly greater than that in the adjacent normal tissue of gastric cancer patients. After RPS15a silencing, the BGC823 cell proliferation rate decreased significantly; most cells were arrested in the G0/G1phase, cell growth was inhibited, and the migration rate was decreased. Colony formation assay showed that the number and sizeof clones in the RPS15a-silenced cells were fewer and smaller, compared to control cells. The nude mouse tumorigenicity testshowed that RPS15a silencing had an inhibitory effect on tumor volume and mice weight. Conclusion: The present study found RPS15a expression to be higher in gastric tumors and its silencing in gastric cancer cells toinhibit the proliferation, growth, and migration thereof. Accordingly, RPS15a may be considered as a potential therapeutic targetin gastric cancer.

Regulation of MUC6 Methylation Correlates with Progression of Gastric Cancer

Ding Shi,Xiao-xia Xi 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.11

Purpose: This study aimed to investigate the mechanistic downregulation of mucin 6 (MUC6) and its influence on the progression of gastric cancer (GC). Materials and Methods: The expression of MUC6 was examined in 40 GC patients. The methylation status of the MUC6 promoter region was investigated using GC cell lines and GC tissue specimens by immunohistochemistry and/or quantitative polymerase chain reaction (qPCR). MUC6 was knocked down in the gastric epithelial cells (GES-1) cell and overexpressed in the SGC7901 cell. The effects of MUC6 knockdown and overexpression on cell migration and invasion were examined using Transwell assays. The effects of demethylation and methylation on MUC6 expression were examined by western blot, qPCR, or double luciferase reporter assays. Results: The expression of MUC6 in GC with lymph node metastasis and poor pathological stage was significantly lower than that in GC without lymph node metastasis and good pathological stage, respectively. While cell migration and invasion were significantly decreased after overexpression of MUC6, these abilities significantly increased after the knockdown of MUC6. The methylation levels of MUC6 in GC tissues and GC cell lines were significantly higher than those in para-cancerous tissues and normal GES. Promoter methylation could significantly reduce the binding of related transcription factors to the MUC6 promoter. The expression of MUC6 increased with the concentration and time of action of demethylation drugs. Conclusion: Expression of MUC6 was regulated by promotor methylation. This methylation of the MUC6 promoter may lead to significant downregulation of MUC6 in GC and promote the progression of GC.

Research on training Strategy of information Technology Application Ability of Normal university students

Shi-mei JIN,Liu-wen DING,Ding-rui LIU,Hui-yan LI ASCONS 2020 IJASC Vol.2 No.4

Background/Objectives: Under the background of educational informatization, the realization of education modernization and information education is the inevitable trend of future education development. As the backbone of the future teacher team, the level of information technology application ability of normal university students is the key to the modernization of future education. Therefore, the ministry of education proposed to strengthen the cultivation of information literacy of normal university students. Methods/Statistical analysis: As the cradle of basic education teachers in yanbian prefecture, yanbian university should strengthen the cultivation of information technology application ability of normal university students and study the current situation of the cultivation. In this paper, two questionnaires were conducted before and after the training of normal university students in yanbian university. Findings: Through the comparison and analysis of the two data by SPSS software, it is found that there is no specific training for normal university students in the training of information technology application ability of normal university students in yanbian university. Teaching less theoretical knowledge; Students have fewer opportunities to practice the problem. Improvements/Applications: Finally, the author puts forward some strategies for the future cultivation of information technology application ability of normal university students in yanbian university. For the training of excellent information teachers to contribute.

Logical Connectivity Prediction Models for VANET based on Nonlinear Regression and ELM : An Example of the AODV Protocol

Haiqing Liu,Licai Yang,Sijing Ding,Yunfeng Shi 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.6

Differing from the physical connectivity of the topology structure, the logical connectivity of VANET considers both the interior network configuration and the external communication environment. Hence, the traditional mathematical analysis and modeling methods which are usually used in physical connectivity research are no longer suitable for the logical connectivity prediction. Taking the AODV protocol as an example, this paper simulates the effects of different road traffic parameters on logical connectivity probability and selects three main effect factors, roadway length, vehicle number and vehicle speed. Furthermore, the inner relation between the logical connectivity and the three road traffic parameters is studied based on data mining technique and then two logical connectivity prediction models are presented, the nonlinear regression-based model and the extreme learning machine-based model. Simulation results show that the two models are both with high accuracy in predicting the network logical connectivity under different road traffic environments.

Measure Correlation Analysis of Network Flow Based On Symmetric Uncertainty

( Shi Dong ),( Wei Ding ),( Liang Chen ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.6

In order to improve the accuracy and universality of the flow metric correlation analysis, this paper firstly analyzes the characteristics of Internet flow metrics as random variables, points out the disadvantages of Pearson Correlation Coefficient which is used to measure the correlation between two flow metrics by current researches. Then a method based on Symmetrical Uncertainty is proposed to measure the correlation between two flow metrics, and is extended to measure the correlation among multi-variables. Meanwhile, the simulation and polynomial fitting method are used to reveal the threshold value between different correlation degrees for SU method. The statistical analysis results on the common flow metrics using several traces show that Symmetrical Uncertainty can not only represent the correct aspects of Pearson Correlation Coefficient, but also make up for its shortcomings, thus achieve the purpose of measuring flow metric correlation quantitatively and accurately. On the other hand, reveal the actual relationship among fourteen common flow metrics.

Influence of LPPS Spraying Parameters on Deposition Efficiency of Zirconia Powder

Shi, Jian-Min,Hu, Zhong-Yin,Huang, Jing-Qi,Ding, Chuan-Xian The Korean Vacuum Society 1997 Applied Science and Convergence Technology Vol.6 No.1

Yttria stabilized zirconia coating is an attractive material for several engineering applications. In order to produce coatings with consistent and reliable performance it is important to understand the influence of spraying parameters on the coating properties and optimize the spraying parameters. In this paper the low pressure plasma spray(LPPS) deposition of as-received zirconia powder has been investigated using simple one-factor-at-a-time approach. The deposition efficiency was chosen to evaluate the melting characteristics of the as-received zirconia powder. The results obtained indicated that the deposition efficiency of zirconia powder is very sensitive to the spraying parameters such as plasma gas flow rate and ranges from 24% to 57% The microstructure and the phase composition of zirconia coating deposited with the different plasma spraying parameters were also examined by SEM and XRD respectively. The relationship between deposition efficiency and the microstructure of zirconia coating was discussed.

Dynamics of waste-to-energy incineration R&D collaboration networks: a social network analysis based on patent data

Ding Ma,Yongsheng Yu,Si-shi Liu,Ya-rui Zhang 한국자원공학회 2017 Geosystem engineering Vol.20 No.2

The municipal solid waste in China is increasing at an unprecedented pace due to the upgrading urbanization and improving living standard. The waste-to-energy (WTE) incineration technology offers a viable and potentially profitable technical solution to this booming phenomenon. The highly complex and systematic nature of WTE technologies have led to the emergence of WTE innovation network in the past decade. This study applies complex network theory as well as social network analysis to investigate the social network structure evolution of WTE technologies in terms of global network properties, major actors’ centrality and collaboration relationships. Based on the data-set covering joint filing patents pertaining to waste heat over the period 2006–2015, this study finds that the overall WTE innovation network exhibits the properties of scale-free network with minority organizations linking to abundant organizations. A large fraction of major actors represent the first-movers in the WTE research and still control the technological trajectories. The collaboration relationships have moved toward higher level of multidisciplinary and larger technological distance. Overall, the evolution of WTE innovation network features a young state of industry with high regime dependence. Finally, some suggestions are proposed to shed light on benign development of WTE innovation network.

A New Modulation to Eliminate the Impact of Overlap Time for Three-Phase Current Source Converters

Ding Hao,Guo Xiaoqiang,He Meiling,Shi Changli,Jia Dongqiang,Chen Chao,Guerrero Josep M. 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

Three-phase current source converter tends to be attractive in future industrial applications. In order to ensure the continuous current for the dc-link inductor of the current source converter (CSC), an overlap time is mandatory for each switch, so as to avoid the over-voltage spike caused by the dc-link inductor current interruption. However, the overlap time results in the undesirable harmonics and current distortion, which fails to comply with the relevant IEEE Standard in terms of power quality. Especially, the negative infl uence caused by overlap time eff ect is much more serious with the increasing of the switching frequency applied to CSC. In order to solve the problem, the impact of the overlap time on the CSC is investigated, and the error resulted from the overlap time is clearly demonstrated. Based on the pulse equivalent principle and the Fourier analysis, the quantitative relationship between the current harmonic and the overlap time is obtained. And then, a new approach to eliminate the impact of overlap time on the CSC is proposed. Finally, the simulation and experimental results are carried out to verify the eff ectiveness of the proposed solution.

N-doped porous carbons with increased yield and hierarchical pore structures for supercapacitors derived from an N-containing phenyl-riched copolymer

Ding-Ming Xue,Shi-Chao Qi,Xin Liu,Yu-Xia Li,Xiao-Qin Liu,Lin-Bing Sun 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

N-doped porous carbon-based materials (NPCMs) with hierarchical pore structures have beenconsidered to be a suitable alternative to meet the ever-increasing demands for supercapacitors;however, the universally low yield of the NPCMs has restricted their practical applications. Herein, aseries of NPCMs with hierarchical pore structures are synthesized with significantly increased yieldsthrough the carbonization of the copolymer made from 2,4,6-tris(chloromethyl)mesitylene and pphenylenediamine. The development of the hierarchical pore structures and the N content of the NPCMsshow opposite dependences on the increasing carbonization temperature. The NPCM exhibits the bestcapacitive ability only if the sufficiently developed hierarchical structures and moderate N content areachieved simultaneously. Therefore, NPCM-600 that is carbonized at 600 C with an excellent yield of53.6% (wt.), large specific surface area of 1778 m2 g 1, and N content of 4.13% (wt.) yields an ideal specificcapacitance of 298 F g 1 at the current density of 1 A g 1 and a perfect cycling stability of the capacitanceafter 10,000 cycles at 10 A g 1. The yield of the NPCM-600 is considerably higher than those for manyother recently reported NPCMs. NPCM-600 also shows better capacitance than those of the otherreported NPCMs, such as NOPC-bis-CN-3 (167 F g 1) and CHCPB-K-600 (260 F g 1).