The Discusion and Optimization Research on HSD17B13 Protein Separation and Purification Technology

Yan Cheng,Zhen Wan,Xiaohong Sun ASCONS 2022 IJASC Vol.4 No.3

Background/Objectives: The Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease that affects nearly a quarter of the world population. Recent studies have shown that there is a close relationship between liver lipid-titrated 17β Hydroxysteroid Dehydrogenase Type 13(HSD17B13)protein and NAFLD. At the same time, the purification of HSD has been trying to find better conditions and methods, at present, the HSD protein separation and purification technology is incomplete, the cost is high, the purity is low, the purification quantity is small and so on. This paper first reviewed the research progress of Steatosis and some basic properties about HSD, such as HSD expression site, Lipid titration function, in vitro catalytic substrates, and the relationship between HSD and liver diseases.. Methods/Statistical analysis: The optimization of HSD purification method is considered be of great help to the further study of its structure and function, so this research put forward two hypotheses to explore its effect on the purification of HSD. The first is to change the kind of tag when expressing plasmid in E. coli, so as to change the adsorption degree of protein with corresponding group and beads, and the second is to change the concentration of different components in Wash Buffer, the aim is to find the best concentration for removing foreign protein and maintaining the adsorption capacity of HSD. Findings: The purity of HSD is closely related to tag species and Wash Buffer concentration in different experimental groups, his-Binding Buffer with 0.5% sodium deoxycholate powder added and Wash Buffer with Tris-HCl 50 mM, NACL 300 mM, Imidazole 40 mM for each component are used. Improvements/Applications: After the better purification effect, the higher purity HSD can pave the way for the future study of its potential substrate and mechanism of action.

Automatic Watering Using Soil Moisture Sensor

Bernardo A. Pangilinan,Allen N. Maroma ASCONS 2020 IJASC Vol.2 No.1

Background/Objectives: In recent years studies were done to determine the relationship of water content of the soil and its electrical resistivity, devices of different application can be construct using these relationship. The following applications benefitted from these findings like automatic water irrigation, soil moisture test for construction and watering system using soil moisture sensor. Methods/Statistical analysis: This study took advantage of the feature of microcontroller to sense varying resistance and controlling the solenoid water valve. The device is equipped with soil moisture sensor that varies its resistance depending on the conductivity of the soil. When the weather is to hot and the soil is dry, the resistance of the soil increases, if the soil is moist resistance of the soil decreases. Findings: The specific value of resistance was set in the microcontroller that signifies the need to water the plant using solenoid water valve. The controller will automatically open the gate valve of the water source, if the set resistance for watering was reached by the sensor. Improvements/Applications: Since water continuously enters the soil, it changes the resistivity of the soil. When the sensor reaches a resistance set in the microcontroller this will trigger the gate valve to close and signifies wet condition of the soil.

Students’ Motivational Beliefs, Values and Goals as Related to Academic Hardiness : Implications to Mathematics Teaching and Learning

Edgardo M. Santos ASCONS 2018 INTERNATIONAL JOURNAL OF EMERGING MULTIDISCIPLINAR Vol.2 No.1

The study examined the relationship between the Academic Hardiness and Motivational Beliefs, Goals and Values of tertiary students towards mathematics learning. The respondents of the study consist of about 167 BS Mathematics third year students of the Bulacan State University during Academic Year 2016-2017. The descriptive – correlational method of research was employed in the study. To gather the necessary data, the researcher adopted two instruments - the Academic Hardiness Scale developed by Benishek and Lopez (2001) and the Learning Motivation Questionnaire, a section from the Motivated Strategies for Learning Questionnaire (MSLQ) developed by Duncan and McKeachie (2005). Pearson correlation analysis and Test of significance were used to interpret the results. The study showed that among the three related attitudes of Academic Hardiness (Commitment, Control and Challenge), the students posted the highest mean in the Commitment domain. Seemingly, the difficulty posed by mathematics has not negated students’ interest and involvement in doing their mathematical tasks. Regarding the status of the students’ Motivational Beliefs, Goals and Values toward mathematics learning, results yielded a high overall mean rating reflective of the high ratings received in almost all the motivational constructs: Intrinsic Goal Orientation, Extrinsic Goal Orientation, Task Value, Controls of Learning Beliefs, Self-Efficacy for Learning and Performance except Test anxiety. The highest perceived mean is on Task Value which demonstrates the respondents expressed interest in mathematics and their acknowledge importance of the subject in their preparation to life-long learning. For Test Anxiety, perceived mean rating is average. This result simply reiterates the psychological distress experienced by students when taking examinations in mathematics. Lastly, analysis also showed that students’ Academic Hardiness and the identified motivational constructs has significant positive correlation with each other.

Case Study for Hybrid Physical Education Class in Korea

Do-Jin Kim,Kyung-Tae-Eo ASCONS 2022 IJBSA Vol.4 No.1

Background/Objectives: The purpose of this study was as a case study for the application of hybrid classes in physical education classes, centered on the physical education teachers. Methods/Statistical analysis An unstructured questionnaire was prepared so that the research subjects could understand and interview the research topic naturally. The interview contents of the case study subjects were described and analyzed centered on the researcher. Findings: A model of hybrid physical education classes is needed. Smart devices are needed for each student, and teachers need to be empowered. It is necessary to share content related to physical education and solve the copyright problem. Improvements/Applications: It is judged that research on content creation and copyright of hybrid classes is necessary. If the advantages of hybrid classes are utilized in physical education classes, it is thought that it will have a positive effect not only when face-to-face classes are difficult, but also in various situations.

Research on the construction and practice of blended teaching model based on flipped classroom

Xin QI,Shi-mei JIN ASCONS 2022 IJASC Vol.4 No.2

Background/Objectives: In the background of the information age, the blended teaching mode is more suitable for students at this stage, but for college students, the simple blended teaching can not guarantee the students' learning enthusiasm, and there are still cases of mixed credits and improper learning attitude. Methods/Statistical analysis: This study introduces the concept of flipped classroom, combines it with blended teaching, and builds a blended teaching mode based on flipped classroom, and takes the elective course of "Modern Education Technology" as an example to practice teaching. Findings: The teaching mode can improve the teaching effect and The teaching mode can improve teaching effectiveness and enhance students' interest in learning. Improvements/Applications: In implementing the flipped classroom-based blended teaching model, universities should pay attention to the combination of students' main role and teachers' guidance, ensure the communication channels between teachers and students, and pay attention to the diversity of evaluation methods.

Comparative Analysis of Consumer, Producer, and Expert Perceptions of GM Agricultural Products

Ji-hyun, Lee,Yu-seon, Jang,Hye-won, Jeong,Seon-min, Jin,Sung-Won, Lee ASCONS 2021 INTERNATIONAL JOURNAL OF EMERGING MULTIDISCIPLINAR Vol.5 No.3

Background/Objectives: The purpose of this study is to compare and analyze the differences between producers and experts based on consumers' perceptions of GM agricultural products. Methods/Statistical analysis: To this end, we analyzed how consumers, producers, and experts' perceptions differ with group-specific comparative analysis data on genetically modified agricultural products previously investigated. Findings: As a result of the analysis, it was found that consumers thought genetically modified agricultural products and foods were generally harmful to the human body compared to producers and experts, and fear was also great. When analyzing consumers' perception of whether a genetically modified food labeling system is needed through additional analysis, consumers think it should be marked on the product through a genetically modified food labeling system, but they usually purchase it without checking whether it is marked. Improvements/Applications: Through comparative analysis of consumer perception, a strategic plan for changing consumer perception was proposed along with institutional supplementation of the genetic modification labeling system in the future.

Effect of Fasting Weight Training on Obesity Indicators and Blood Lipids on Korean

Do-Jin Kim ASCONS 2021 IJBSA Vol.3 No.4

Background/Objectives: The purpose of this study was to examine changes in obesity indicator and blood lipids by participating in weight training after maintaining a fasting for 12 hours in obese middle-aged women for 8 weeks. Methods/Statistical analysis: 8-week exercise program was conducted by dividing into 10 people in the fasting weight training group(WG) and 10 people in the control group(CG). PASW 18.0 Statistical programs were used to present the descriptive statistics of the measurement variables. Comparisons of the two groups were analyzed by 2-way ANOVA. Findings: There was a significant difference between the WG and the CG in TG, TC, HDL and leptin(p<05), but there was no significant difference in irisin(p>.05). Improvements/Applications: The purpose of this study was to examine the effect of fasting exercise on obese middle-aged women. Through this exercise, significant results were obtained in obesity indicator and blood lipid. If future research is conducted through changes in exercise duration and fasting time, meaningful results will be provided.

The Effects of Life-sustaining Treatment Preferences on Geriatric Depression : Centered on the Mediating Effects of Ego Integrity and Social Support

S. H. Kim,O. S. Cho ASCONS 2021 IJBSA Vol.3 No.1

Automated Driver’s Assessment and Driving Simulator System

Rionel Belen Caldo,Joemar Palomaria ASCONS 2019 IJASC Vol.1 No.1

Background/Objectives: The present invention generally relates to a driver’s assessment and training simulator system designed for the improvement of the driver’s screening and qualification procedures of TESDA prior to the license issuance and release in LTO. Methods/Statistical analysis: Driving Assessment and Simulation Hub (HUB) would like to propose a solution to preparing problems by designing, developing and implementing an Automated Driver's Assessment and Driving Simulator System. Findings: The present invention discloses an electronic system comprising three (3) main software components specifically, an electronic driving knowledge examination system with biometrics registration validation feature and touchscreen terminals, a driving simulation testing system with automatic scoring and up to thirty (30) heads per hour testing capacity, and a decision support system for the evaluation of the two exam results. Improvements/Applications: Driving Assessment and Simulation Hub (DASH) project team is making the Philippines a happy and safe place to drive in.

Interactive 3D Directory: University of La Salette

Dreivan B. Orprecio,Harvie A. Bañares,Lary Jay C. Guzman,Jielie C. Conjusta,Genesis S. Jose ASCONS 2020 IJASC Vol.2 No.2

Background/Objectives: There is an impact of multimedia on engineering students. They use interactive multimedia to simulate scenarios in computer applications, electronics, and later implement and calibrate their designs in an actual environment. Architecture students use interactive multimedia for design presentations. Methods/Statistical analysis: This study focuses on the development of the Interactive 3D Directory for the University of La Salette. As Computer Engineers, we want to showcase our skills and knowledge to the locality and to help the end-users, i.e., visitors, new students, and transferees, to locate buildings and departments in this University. We used Unity3d, c# programming, and SketchUp to develop this project. This study used the survey method to acquire related data. Questionnaires were floated before and after the implementation of the system. Findings: The results found out that most of the time, new students, transferees, visitors ask directions and feel the need for a system that gives accurate directions. The results also found out that most of the respondents agree that the developed system was effective and easy to use. Improvements/Applications: Based on the study findings, the researchers suggest that the administration introduce the established program on all of the region's high school campuses to help visitors, transferees, and new students identify offices, facilities, and departments.