Genetic Variability and Bottleneck Analysis of Jakhrana Goats by Microsatellite DNA Fingerprinting

Dinesh Kumar,A. K. Pandey,S. P. S. Ahlawat,Geetu Malik,Rekha Sharma,Digpal Singh Gour,S. P. Dixit,N. K. Verma,R. A. K. Aggarwal,Neelam Gupta,B. N. Shahi,S. C. Gupta 한국유전학회 2005 Genes & Genomics Vol.27 No.3

Tribochemistry of contact interfaces of nanocrystalline molybdenum carbide films

Kumar, D. Dinesh,Kumar, N.,Panda, Kalpataru,Kamalan Kirubaharan, A.M.,Kuppusami, P. Elsevier 2018 APPLIED SURFACE SCIENCE - Vol.447 No.-

<P><B>Abstract</B></P> <P>Transition metal carbides (TMC) are known for their improved tribological properties and are sensitive to the tribo-atmospheric environment. Nanocrystalline molybdenum carbide (MoC) thin films were deposited by DC magnetron sputtering technique using reactive CH<SUB>4</SUB> gas. The friction and wear resistance properties of MoC thin films were significantly improved in humid-atmospheric condition as compared to high-vacuum tribo-condition. A comprehensive chemical analysis of deformed contact interfaces was carried out by X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDX) and Raman spectroscopy. XPS and Raman spectroscopy showed the formation of stable molybdenum-oxide (MoO), molybdenum carbide (MoC) and amorphous carbon (a-C) tribo-phases. Moreover, during the sliding in humid-atmospheric condition, these phases were extensively deposited on the sliding steel ball counter body which significantly protected against undesirable friction and wear.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Reactive DC magnetron sputtered cubic phase of nanocrystalline MoC thin films. </LI> <LI> Chemical bonding analysis of MoC films using X-ray photoelectron spectroscopy. </LI> <LI> Tribological properties of MoC films in ambient and high-vacuum tribo-atmospheres. </LI> <LI> Comprehensive tribochemical analysis of contact interfaces. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Friction and wear behavior of nanocrystalline MoC films under atmospheric and high-vacuum conditions.</P> <P>[DISPLAY OMISSION]</P>

Poverty Does Not Limit Tobacco Consumption among Tribal Populations: Evidence from Central India

Kumar, Surendra,Kumar, Dinesh,Muniyandi, Malaisamy,Soan, Visheshwar,Pandey, Maya,Mishra, Dinesh Kumar Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Hepatoprotective Activity of Rutin Against Liver Damage Through Interaction with Cytochromes P450 and Antioxidant Enzymes: In-vivo and In-vitro Experimental Data Analysis and Molecular Modelling

( Dinesh Kumar Patel ),( Kanika Patel ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Plant based drugs have numerous phytochemicals mainly belongs to the phenolic flavonoid and carotenoids class chemicals. These phytochemicals play important role in the plant’s defence mechanism and used for the treatment of numerous human disorders in the modern system of medicine. Plants and their derived products contain important class of phytochemicals which are still considered as one of the important sources of materials for the development of noble molecule with better therapeutic goal. Cytochromes P450 enzymes are capable of catalyzing the oxidative biotransformation of numerous drugs in the human body. Rutin is a low molecular weight polyphenolic compound mainly found to be present in various citrus class drugs. Rutin have important physiological functions both in the human body and plants. Methods: To know the beneficial effect of rutin in liver system, various scientific databases have been searched and analyzed in the present investigation to know the therapeutic potential of Rutin in the biological system. Pharmacological importance of rutin in Hepatic system have been searched and analyzed. Molecular simulation database of rutin against cytochrome P-450, superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) have been searched to know the importance of rutin in liver antioxidant systems. Binding energy and type of molecular interactions have been also analyzed in the present investigation through databases analysis to uncoil the molecular mechanism of rutin against hepatic disorders. Results: Scientific research databases analysis revealed the importance of rutin in the hepatic system as higher intake of rutin for 20 days in rat tissues showed improve liver antioxidant level and reduced systemic inflammation and oxidative stress. However, rutin significantly reduced transaminases and phosphatases enzymes in liver and restores oxidative damage. Molecular simulation study database analysis signified the importance of cytochrome P-450 in liver disorders. Further investigation also revealed the importance of the SOD, CAT and MDA in the treatment of hepatic disorders. Conclusions: Present investigation revealed the importance of rutin as hepatoprotective agent and signified their role in the oxidative stress and could be used for the development of newer medicine to treat liver disorders.

Combinatorial Effect of Ligand Aromaticity, Torsion Number and Hydrophobicity on the Predictability of AutoDock for Protein-Ligand Binding Conformation

Dinesh Kumar Sriramulu,이선구 한국생물공학회 2023 KSBB Journal Vol.38 No.1

Molecular docking method helps predict the protein- ligand binding conformation, but its prediction accuracy is still limited and varies depending on the target ligand's structure and physicochemical properties. Therefore, understanding the ligand-dependent prediction accuracy is crucial in efficiently using the docking tool. In this study, we investigated how the prediction accuracy of AutoDock, a popular molecular docking tool, is affected by the aromaticity of the target ligand structure, ligand torsion number, and ligand hydrophobicity. The ligands with an aromatic ring showed substantially lower prediction accuracy than those without an aromatic ring. The ligands with lower torsion number showed higher prediction accuracy in the ligands with and without an aromatic ring. The hydrophobicity of ligands did not significantly influence the prediction accuracy in the case of ligands with an aromatic ring structure. However, the ligands without ring structure showed a clear difference in prediction accuracy depending on their hydrophobicity. These results are expected to be employed as a reference in molecular docking studies using AutoDock.

Modulating the Biomechanical Culture Environment for Improved Differentiation of Human Mesenchymal Stem Cells

Dinesh Kumar Patel,Yu-Ri Seo,Ki-Taek Lim 강원대학교 산림과학연구소 2018 강원대학교 산림과학연구소 학술대회 Vol.2018 No.09

Human mesenchymal stem cells (hMSCs) are critical for numerous groundbreaking therapies in the field of regenerative medicine. Nanoscale topography of artificial substrates can greatly influence the fate of stem cells including adhesion, proliferation, and differentiation. Thus the design and manipulation of reduced graphene oxide (rGO)-based nanosheets and its electrical properties are of great importance to realize graphene-based electronics as a strategy in stem cells and tissue engineering applications. In this report, we propose that electro-conductive graphene oxide nanosheets with pulsed electromagnetic fields (PEMFs) are an efficient platform for modulating and enhancing structure and function of hMSCs. Using a self-assembly method, we successfully coated graphene oxide (GO) on glass for fabricating GO nanocomposite and had tunable electrical conductivity of graphene oxide sheets. The hMSCs grown on the rGO films under PEMFs showed increased adhesion, indicated by a large number of focal adhesions, and higher mineralization markers of the extracellular matrix and their osteogenic differentiation in a controlled manner through vinculin, vimentin, osteopontin (OPN), fibronectin, calmodulin (caM) expressions, and differential hierarchical clustering by microarray in hMSCs. Here we show that rGO with PEMFs as a graphene-based cell stimulator provides a promising biocompatible nanocomposite that does not hamper the proliferation of hMSCs and accelerates their specific differentiation into bone cells. Our bottom-up biomechatronic approach of tuning the rGO-sheet properties provides a path to a broad new class of graphene-based materials and their use in a variety of applications.

Anti allergic and anti-inflammatory effects of the fruits of Randia dumetorum Lamk

Dinesh kumar,Satish C. Mudgade,Zulfiqar Ali Bhat,Santosh S. Bhujbal,R. Rub,이철훈,손은화 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.3

Randia dumetorum Lamk. commonly known as Madana in Sanskrit and widely distributed throughout India. In Ayurveda, Randia dumetorum Lamk. is used in treatment asthma, rhinitis, bronchatitis, cold, cough, pain,inflammation etc. The aim of this study: was to assess the anti allergic effects of the fruit of Randia dumetorum Lamk. Allergy and anaphylaxis are the most responsible factor for diseases like asthma, rhinitis, bronchatitis, cold,cough, pain, inflammation etc. Asthma is an inflammatory disease of lungs and the hallmarks of the disease are the increased inflammatory cell infiltration into the airways and poor respiratory function although there is a possibility that Randia dumetorum Lamk. may have an inhibitory effect on lung inflammation and allergic mediators. The effects of Randia dumetorum Lamk. on allergy and asthma have not been fully investigated. In the present study, we investigated the antiallergic and antiinflammatory activity of Randia dumetorum Lamk. extract and its fraction on milk induced leucocytosis and eosinophilia in mice, passive paw anaphylaxis and mast cell degranulation in rat models. Randia dumetorum Lamk. extract was obtained from dried and powdered fruits of Randia dumetorum Lamk. using 95% methanol and its fractions were obtained by using increasing polarity of solvents like Petroleum ether, chloroform,ethylacetate and methanol. Milk was used to induce leucocytosis and eosinophilia in mice model, eggalbumin was used as sensitizer in passive paw anaphylaxis in rat model and clonidine was used to degranulate the mast cells in rat model. We examined the effects of Randia dumetorum Lamk. extract and its fraction on milk induced leucocytosis and eosinophilia in mice, passive paw anaphylaxis and mast cell degranulation in rats. The extract and its fractions significantly inhibited leukocytosis and eosinophilia in blood of mice. The extract and its fractions also significantly inhibited the passive paw oedema and mast cell degranulation in rats. Methanol extract showed dose dependent significant activity (**p<0.01). Amongst the fractions, chloroform fraction showed the most significant activity followed by ethyl acetate and residual methanol fraction with intermediate activity and finally the petroleum ether fraction with the least or negligible activity (*p<0.05, **p<0.01). These results suggest the possibility of extract and its fraction can exert suppressive effects on allergy, inflammation and asthma and may provide evidence that Randia dumetorum Lamk. is a useful agent for the treatment of allergic airway disease. Hence it would be a good path for the isolation of most bioactive principle for the management of allergic airway disease.

Plasmon-assisted and visible-light induced graphene oxide reduction and efficient fluorescence quenching

Kumar, Dinesh,Kaur, Sandeep,Lim, Dong-Kwon The Royal Society of Chemistry 2014 Chemical communications Vol.50 No.88

<P>Here we report a new and efficient synthetic method for reduced graphene oxide using visible-light and plasmonic nanoparticles at room temperature. The r-GO prepared using visible light showed excellent fluorescence quenching properties and target detection capabilities.</P> <P>Graphic Abstract</P><P>The use of plasmonic nanoparticles accelerated visible-light induced reduction of graphene oxide at room temperature. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc05136j'> </P>

Health beneficial properties and protective effect of linarin against various form of hepatic failure: An phytotherapeutic approach

Dinesh Kumar PATEL,Kanika PATEL 한국간담췌외과학회 2022 Annals of hepato-biliary-pancreatic surgery Vol.26 No.-

Reduced graphene oxide modified smart conducting paper for cancer biosensor

Kumar, Saurabh,Kumar, Suveen,Srivastava, Saurabh,Yadav, Birendra K.,Lee, Seung H.,Sharma, Jai G.,Doval, Dinesh C.,Malhotra, Bansi D. Elsevier 2015 Biosensors & bioelectronics Vol.73 No.-

<P><B>Abstract</B></P> <P>We report results of the studies relating to the fabrication of a paper based sensor comprising of poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and reduced graphene oxide (RGO) composite. The effect of various solvents like methanol, ethylene glycol and H<SUB>2</SUB>SO<SUB>4</SUB> on the electrical conductivity of PEDOT:PSS coated Whatman paper has been investigated. The conductivity of this solution processed conducting paper significantly increases from ~1.16×10<SUP>−4</SUP> Scm<SUP>−1</SUP> up to ~3.57×10<SUP>−2</SUP> Scm<SUP>−1</SUP> (~300 times) on treatment with ethylene glycol. The observed significant increase in electrical conductivity is due to conformational rearrangement in the polymer and is due to strong non-covalent cooperative interaction between PEDOT and the cellulose molecules. Further, incorporation of RGO into the conducting paper results in improved electrochemical performance and signal stability. This paper electrode is a promising alternative over the expensive conventional electrodes (ITO, gold and glassy carbon), that are known to have limited application in smart point-of-care (POC) devices. This low cost, flexible and environment friendly conducting paper based biosensor utilized for cancer biomarker (carcinoembryonic antigen, CEA) detection reveals high sensitivity of 25.8µAng<SUP>−1</SUP> mLcm<SUP>−2</SUP> in the physiological range, 1–10ngmL<SUP>−1</SUP>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Solution processed reduced graphene oxide modified conducting paper sensor is reported for cancer detection. </LI> <LI> This paper sensor is exceptionally low cost, flexible and disposable. </LI> <LI> It exhibits high sensitivity of 25.8µAng<SUP>−1</SUP> mLcm<SUP>−2</SUP> in the detection range of 1–10ngmL<SUP>−1</SUP> for cancer biomarker (carcinoembryonic antigen, CEA) detection. </LI> <LI> This conducting paper electrode is a promising alternative over the expensive conventional electrodes (ITO, gold and glassy carbon) for application to POC devices. </LI> </UL> </P>