여대생의 성격 5요인과 이상섭식행동, 우울 및 삶의 만족도의 상관관계

강혜빈,고현진,김성연,박서인,박수연,박은별,유미연,이세인,조영은,Deng Wei,김석선,길민지 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this study is to examine the correlations big five personality traits, abnormal eating behavior, depression and, life satisfaction among female university students. Methods: The subjects for this study were female university students in Seoul, Korea. The data were collected from 151 female university students from August 13, 2019 to August 22, 2019. They were asked to fill out Big Five Inventory- Korean version(BFI-K), The Korean Center for Epidemiologic Studies Depression Scale(KCES-D), The Korean Version of Eating Attitudes Test-26(KEAT-26), and, Satisfaction With Life Scale(SWLS). The collected data were analyzed using descriptive statistics, T-test, ANOVA, and Pearson’s correlation coefficient. Result: First, College students’ nervousness among the five personality traits was significantly associated with depression and life satisfaction. Second, College students’ agreeableness among the five personality traits was significantly associated with abnormal eating behavior and life satisfaction. Third, College students’ abnormal eating behaviors were significantly associated with depression. Conclusion: These results suggest that female university students with higher level of nervousness are vulnerable to depression. And, it suggests that female university students with higher level of agreeableness are vulnerable to abnormal eating behavior. The intervention to consider five personality traits may improve mental health among female university students.

Growth hormone and receptor gene mutations in Chinese Banna miniature pig

Deng, J.Z.,Hao, L.L.,Li, M.T.,Lang, S.,Zeng, Y.Z.,Liu, S.C.,Zhang, Y.L. The Korean Society for Integrative Biology 2011 Animal cells and systems Vol.15 No.4

The Banna miniature pig (BNMP) is a representative miniature pig breed in China. Even though BNMP dwarfism is obvious, its underlying causative mutations remain unknown. In this study, the BNMP and Large White pig (LWP) serum growth hormone (GH) and insulin-like growth factor (IGF-1) levels were detected by ELISA and compared. BNMP serum IGF-1 levels were significantly lower than LWP levels (P<0.05). The miniature condition may arise from mutations in the GH and GH receptor (GHR) genes. Therefore, GH and GHR cDNA from the BNMP were cloned into a pMD18-T vector by RT-PCR using the total RNA obtained from the BNMP's pituitary and liver tissues. Sequencing results indicated that the open reading frame of the BNMP GH gene is composed of a 26-residue signal peptide and a 191-residue mature peptide. The coding sequence of the BNMP GHR gene contained 639 amino acids, including a signal peptide that is 18 amino acids long. Two amino acid substitutions, A09V and R22Q, were found in the signal peptide of the GH gene. Additionally, the S104P mutation was found in the BNMP's mature GH protein. Four mutations in the cytoplasmic domain of GHR may influence the downstream signal transduction of GHR, which needs further experimental evidence.

Growth hormone and receptor gene mutations in Chinese Banna miniature pig

J.Z. Deng,L.L. Hao,M.T. Li,S. Lang,Y.Z. Zeng,S.C. Liu,Y.L. Zhang 한국통합생물학회 2011 Animal cells and systems Vol.15 No.4

The Banna miniature pig (BNMP) is a representative miniature pig breed in China. Even though BNMP dwarfism is obvious, its underlying causative mutations remain unknown. In this study, the BNMP and Large White pig (LWP)serum growth hormone (GH) and insulin-like growth factor (IGF-1) levels were detected by ELISA and compared. BNMP serum IGF-1 levels were significantly lower than LWP levels (P<0.05). The miniature condition may arise from mutations in the GH and GH receptor (GHR) genes. Therefore, GH and GHR cDNA from the BNMP were cloned into a pMD18-T vector by RT-PCR using the total RNA obtained from the BNMP’s pituitary and liver tissues. Sequencing results indicated that the open reading frame of the BNMP GH gene is composed of a 26-residue signal peptide and a 191-residue mature peptide. The coding sequence of the BNMP GHR gene contained 639 amino acids, including a signal peptide that is 18 amino acids long. Two amino acid substitutions, A09V and R22Q, were found in the signal peptide of the GH gene. Additionally, the S104P mutation was found in the BNMP’s mature GH protein. Four mutations in the cytoplasmic domain of GHR may influence the downstream signal transduction of GHR, which needs further experimental evidence.

α-Cyperone Alleviates Lung Cell Injury Caused by Staphylococcus aureus via Attenuation of α-Hemolysin Expression

( Luo M ),( J Qiu ),( Y Zhang ),( J Dong ),( H Li ),( B Leng ),( Q Zhang ),( X Dai ),( X Niu ),( S Zhao ),( X Deng ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.8

In this study, we aimed to evaluate the effect of a-cyperone on S. aureus. We used a hemolysin test to examine the hemolytic activity in supernatants of S. aureus cultured with increasing concentrations of a-cyperone. In addition, we evaluated the production of a-hemolysin (Hla) by Western blotting. Real-time RT-PCR was performed to test the expression of hla (the gene encoding Hla) and agr (accessory gene regulator). Furthermore, we investigated the protective effect of a-cyperone on Hla-induced injury of A549 lung cells by live/dead and cytotoxicity assays. We showed that in the presence of subinhibitory concentrations of a-cyperone, Hla production was markedly inhibited. Moreover, a-cyperone protected lung cells from Hla-induced injury. These findings indicate that a-cyperone is a promising inhibitor of Hla production by S. aureus and protects lung cells from this bacterium. Thus, a-cyperone may provide the basis for a new strategy to combat S. aureus pneumonia.

Graphitization of graphene oxide films under pressure

Chen, Xianjue,Deng, Xiaomei,Kim, Na Yeon,Wang, Yu,Huang, Yuan,Peng, Li,Huang, Ming,Zhang, Xu,Chen, Xiong,Luo, Da,Wang, Bin,Wu, Xiaozhong,Ma, Yufei,Lee, Zonghoon,Ruoff, Rodney S. Elsevier 2018 Carbon Vol.132 No.-

<P><B>Abstract</B></P> <P>Lightweight, flexible graphite foils that are chemically inert, high-temperature resistant, and highly electrically and thermally conductive can be used as component materials in numerous applications. “Graphenic” foils can be prepared by thermally transforming graphene oxide films. For this transformation, it is desirable to maintain a densely packed film structure at high heating rates as well as to lower the graphitizing temperatures. In this work, we discuss the pressure-assisted thermal decomposition of graphene oxide films by hot pressing at different temperatures (<I>i.e.</I>, 300 °C, 1000 °C, or 2000 °C). The films pressed at 1000 °C or 2000 °C were subsequently heated at 2750 °C to achieve a higher degree of graphitization. The combination of heating and pressing promotes the simultaneous thermal decomposition and graphitic transformation of G-O films. Films pressed at 2000 °C as well as films further graphitized at 2750 °C show high chemical purity, uniformity, and retain their flexibility. For films pressed at 2000 °C and then further heated at 2750 °C, the mechanical performances outperform the reported values of the “graphite” foils prepared by calendering exfoliated graphite flakes; the electrical conductivity is ∼3.1 × 10<SUP>5</SUP> S/m and the in-plane thermal conductivity is ∼1.2 × 10<SUP>3</SUP> W/(m·K).</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants

Wang, X.-T.,Zhang, H.,Zhao, C.-J.,Li, J.-Y.,Xu, G.-Y.,Lian, L.-S.,Wu, C.-X.,Deng, Xuemei Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.2

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.

New Evidence of Alleles (V199I and G52S) at the PRKAG3 (RN) Locus Affecting Pork Meat Quality

Chen, J.F.,Dai, L.H.,Peng, J.,Li, J.L.,Zheng, R.,Zuo, B.,Li, F.E.,Liu, M.,Yue, K.,Lei, M.G.,Xiong, Y.Z.,Deng, C.Y.,Jiang, S.W. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4

The porcine PRKAG3 (RN) gene encodes the regulatory gamma subunit of adenosine monophosphate-activated protein kinase (AMPK), which is a good candidate gene affecting meat quality. In this study, the effects of two missense mutations A595G (Ile199Val) and G154A (Gly52Ser) in porcine PRKAG3 gene on meat quality traits were studied in M. Longissimus dorsi (LD), M. Semispinalis capitis (SC) and M. Biceps femoris (BF) from different populations of 326 pigs. The PRKAG3 alleles 199I, 199IV, 52S and 52G were identified with PCR-RFLPs and all genotypes - 199I/199I, 199I/199V, 199V/199V, 52S/52S, 52S/52G and 52G/52G - were found. The frequency of V allele was larger than that of I allele in all populations. I allele frequency was zero in Chinese Meishan pigs (population D) especially. G allele frequency was larger than that of S allele in all populations except Large White (population A). Both variations at the PRKAG3 locus significantly affected these meat quality traits. The pork meat quality has not previously been established in Meishan or crosses thereof. The results suggested that generally pH of LD, SC and BF was higher in Meishan pigs than that in other populations. Moreover, Meishan pigs showed higher water-holding capacity and intramuscular fat (IMF), lower water content and water loss percentage compared to other populations in terms of the two variations. The results present here supply new evidence that alleles V199I and G52S at the PRKAG3 locus affect pork meat quality and provide useful information on pork production.

Membrane for the Removal of Volatile Organic Compounds from Air

Deng, S.,Lang, K.,Wang, J.,Tremblay, A.,Matsuura, T. The Membrane Society of Korea 1997 멤브레인 Vol.7 No.1

Porous asymmetric membranes were prepared from polyetherimide polymer by the phase-inversion technique under different conditions. The performance of the membranes was tested for the removal of acetone vapour from nitrogen. A membrane which showed a high acetone permeability and a high selectivity was chosen and tested further for the separation of different organic vapours from nitrogen. The molecular structure of organic vapours and the selectivity were correlated. A strong correlation was also found between the chromatographic retention time of the organic vapour and the selectivity. These experimental results led to the conclusion that the sorption is the factor governing the separation of volatile organic compounds from nitrogen. A membrane was also prepared by coating the surface of a porous polyetherimide membrane with silicone rubber. The performance of membranes with and without silicone rubber coating was compared.

Large-Pore Apertures in a Series of Metal-Organic Frameworks

Deng, H.,Grunder, S.,Cordova, K. E.,Valente, C.,Furukawa, H.,Hmadeh, M.,Gandara, F.,Whalley, A. C.,Liu, Z.,Asahina, S.,Kazumori, H.,O'Keeffe, M.,Terasaki, O.,Stoddart, J. F.,Yaghi, O. M. American Association for the Advancement of Scienc 2012 Science Vol.336 No.6084

<P>We report a strategy to expand the pore aperture of metal-organic frameworks (MOFs) into a previously unattained size regime (>32 angstroms). Specifically, the systematic expansion of a well-known MOF structure, MOF-74, from its original link of one phenylene ring (I) to two, three, four, five, six, seven, nine, and eleven (II to XI, respectively), afforded an isoreticular series of MOF-74 structures (termed IRMOF-74-I to XI) with pore apertures ranging from 14 to 98 angstroms. All members of this series have noninterpenetrating structures and exhibit robust architectures, as evidenced by their permanent porosity and high thermal stability (up to 300C). The pore apertures of an oligoethylene glycol-functionalized IRMOF-74-VII and IRMOF-74-IX are large enough for natural proteins to enter the pores.</P>

대기중의 휘발성 유기물 제거용 분리막

Deng S.,Lang K.,Wang J.,Tremblay A.,Matsuura T. 한국막학회 1997 멤브레인 Vol.7 No.1

Porous asymmetric membranes were prepared from polyetherimide polymer by the phase-inversion technique under different conditions. The performance of the membranes was tested for the removal of acetone vapour from nitrogen. A membrane which showed a high acetone permeability and a high selectivity was chosen and tested further for the separation of different organic vapours from nitrogen. The molecular structure of organic vapours and the selectivity were correlated. A strong correlation was also found between the chromatographic retention time of the organic vapour and the selectivity. These experimental results led to the conclusion that the sorption is the factor governing the separation of volatile organic compounds from nitrogen. A membrane was also prepared by coating the surface of a porous polyetherimide membrane with silicone rubber. The performance of membranes with and without silicone rubber coating was compared.