AcuD Gene Knockout Attenuates the Virulence of Talaromyces marneffei in a Zebrafish Model

( Jiao Feng ),( Zhiwen Chen ),( Liya He ),( Xing Xiao ),( Chunmei Chen ),( Jieming Chu ),( Eleftherios Mylonakis ),( Liyan Xi ) 한국균학회 2019 Mycobiology Vol.47 No.2

Talaromyces marneffei is the only dimorphic species in its genus and causes a fatal systemic mycosis named talaromycosis. Our previous study indicated that knockdown of AcuD gene (encodes isocitrate lyase of glyoxylate bypass) of T. marneffei by RNA interference approach attenuated the virulence of T. marneffei, while the virulence of the AcuD knockout strains was not studied. In this study, T. marneffei-zebrafish infection model was successfully established through hindbrain microinjection with different amounts of T. marneffei yeast cells. After co-incubated at 28_C, the increasing T. marneffei inoculum doses result in greater larval mortality; and hyphae generation might be one virulence factor involved in T. marneffeizebrafish infection. Moreover, the results demonstrated that the virulence of the DAcuD was significantly attenuated in this Zebrafish infection model.

A Study on Optimization Strategies of Enterprises Storage Management

Li Chunmei,Feng Dan,Zhou Gang 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.2

Today, when market competition becomes increasingly fierce, improving the efficiency of storage services becomes a key for chemical companies to win the trade war. Focusing on the improvement of internal storage efficiency of enterprises, aiming at the characteristics that production enterprises for primary chemicals rely on economies of scale to make profit, according to industry conditions and forecasts of demand for market of chemical products based on logistic curve, and analyzing the difference between the storage scales of different enterprises, this paper proposes that general-scale emerging chemical enterprises should make full use of outsourcing resources to achieve economies of scale of storage; while in respect of mature super large enterprises, through using AHP method, object programming with priority and other methods, this paper proposes that enterprises’ own storage systems should be constructed and improved so as to achieve significant improvement of storage efficiency.

Apoptosis and autophagy of muscle cell during pork postmortem aging

Li Chunmei,Yin Xialian,Xue Panpan,Wang Feng,Song Ruilong,Song Qi,Su Jiamin,Zhang Haifeng 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2

Objective: Pork is an important source of animal protein in many countries. Subtle physiochemical changes occur during pork postmortem aging. The changes of apoptosis and autophagy in pork at 6 h to 72 h after slaughter were studied to provide evidence for pork quality. Methods: In this article, morphological changes of postmortem pork was observed through Hematoxylin-eosin staining, apoptotic nuclei were observed by TdT-mediated dUTP nick end labeling assay, protein related to apoptosis and autophagy expressions were tested by western blot and LC3 level were expressed according to immunofluorescence assay. Results: In this study, we found the occurrence of apoptosis in postmortem pork, and the process was characterized by nucleus condensation and fragmentation, formation of apoptotic bodies, increase in apoptosis-related Bax/Bcl-2 levels, and activation of caspases. Autophagy reached its peak between 24 and 48 h after slaughter, accompanied by the formation of autophagosomes on the cell membrane and expression of autophagy-related proteins beclin-1, P62, LC3-I, LC3-II, and ATG5. Conclusion: Obvious apoptosis was observed at 12 h and autophagy reached its peak at 48 h. The present work provides the evidence for the occurrence of apoptosis and autophagy during postmortem aging of pork. In conclusion, the apoptosis and autophagy of muscle cells discovered in this study have important implications for pork in the meat industry.

Observer Design for A Class of Uncertain State-Delayed Nonlinear Systems

Junwei Lu,Chunmei Feng,Shengyuan Xu,Yuming Chu 대한전기학회 2006 International Journal of Control, Automation, and Vol.4 No.4

This paper deals with the observer design problem for a class of state-delayed nonlinear systems with or without time-varying norm-bounded parameter uncertainty. The nonlinearities under consideration are assumed to satisfy the global Lipschitz conditions and appear in both the state and measured output equations. The problem we address is the design of a nonlinear observer such that the resulting error system is globally asymptotically stable. For the case when there is no parameter uncertainty, a sufficient condition for the solvability of this problem is derived in terms of linear matrix inequalities and the explicit formula of a desired observer is given. Based on this, the robust observer design problem for the case when parameter uncertainties appear is considered and the solvability condition is also given. Both of the solvability conditions obtained in this paper are delay-dependent. A numerical example is provided to demonstrate the applicability of the proposed approach.

A Comparison of Developmental Trajectories of Prepulse Inhibition between Male and Female Rats

Yang Yang,Yun'ai Su,Chunmei Guo,Yu Feng,Jitao Li,Tianmei Si 대한정신약물학회 2010 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.8 No.3

Objective: Various studies have demonstrated that childhood and adolescence are critical periods of brain development characterized by both dramatic changes and sexual dimorphism in anatomy, physiology and cognition. Prepulse inhibition (PPI) is an operational model of sensorimotor gating process, but data concerning the interactions of sex and normal ontogeny of PPI is of a particular paucity. Methods: We led a longitudinal study to compare the PPI between male and female Sprague-Dawley rats during postnatal day 23 to 43. Results: We found that the PPI of both male and female rats increased during this period. We also found that the PPI of male and female rats had different developmental trajectories when the prepulse intensity was 20 dB [A] above background, a result similar with a previous study done in humans. Conclusion: The finding confirms the face validity of PPI as a model of information processing across species and supports its utilization for investigation of potential aetiological factors implicated in mental disorders.

An Improved and Efficient Analytical Model for Magnetic Field Calculation in Linear Permanent Magnet Machines

Baocheng Guo,Rong Guo,Hengzai Hu,Jianping Shi,Chunmei Feng 한국자기학회 2022 Journal of Magnetics Vol.27 No.1

A simple and improved analytical model (AM) for linear permanent magnet synchronous machine (LPMSM) based on the correction factor is presented in this paper. A slotless linear permanent magnet machine (PLPM) with AM developed in Cartesian coordinate is selected to investigate. Consequently, the fitting equations of curvature factors for the permanent magnet and armature are obtained. The subdomains for LPMSM are simplified from 6 down to 3 domains based on curvature equations. The magnetic flux density and force show that the proposed approach agrees with the finite element (FE) model. Moreover, the reduced calculation domains and harmonic orders make AM proposed in the paper much faster. The main contribution of the work is to present a simple strategy for coordinate transformation and calculation strategy for LPMSM, which provides guideline for designers to investigate the machines developed in Cartesian coordinate.

Tizoxanide induces autophagy by inhibiting PI3K/Akt/ mTOR pathway in RAW264.7 macrophage cells

Jiaoqin Shou,Mi Wang,Xiaolei Cheng,Xiaoyang Wang,Lifang Zhang,Yingchun Liu,Chenzhong Fei,Chunmei Wang,Feng Gu,Feiqun Xue,Juan Li,Keyu Zhang 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.2

As the main metabolite of nitazoxanide, tizoxanide(TIZ) has a broad-spectrum anti-infective effect againstparasites, bacteria, and virus. In this study, we investigatedthe effects of TIZ on autophagy by regulating the PI3K/Akt/mTOR signaling pathway. RAW264.7 macrophage cellswere treated with various TIZ concentrations. Cell viabilityassay, transmission electron microscope, and immunofluorescencestaining were used to detect the biological functionof the macrophage cells, and the expression levels of theautophagy pathway-related proteins were measured by Westernblot. Results revealed that TIZ promoted the conversionof LC3-I to LC3-II, the formation of autophagy vacuoles,and the degradation of SQSTM1/p62 in a concentration- andtime-dependent manner in RAW264.7 cells. Treatment withTIZ increased the Beclin-1 expression level and inhibitedPI3K, Akt, mTOR, and ULK1 activation. These effects wereenhanced by pretreatment with rapamycin but attenuated bypretreatment with LY294002. In addition, the conversion ofLC3-I to LC3-II was observed in Vero, 293T, and HepG2cells treated with TIZ. These data suggested that TIZ mayinduce autophagy by inhibiting the Akt/mTOR/ULK1 signalingpathway in macrophages and other cells.