하와이의 밤바다 외 3편

정지현(Christine Chung) 문예운동사 2014 문예운동 Vol.- No.122

한국판 부모보고형 유아 정서·행동 평가시스템 타당화 연구

정유선(Chung, You Sun),안명희(Ahn, Christine Myung Hee) 한국영유아교원교육학회 2021 유아교육학논집 Vol.25 No.2

본 연구에서는 한국판 부모보고형 유아 정서·행동 평가시스템(The Korean Behavior Assessment System for Children-2, Parent Rating Scales-Preschool Form: K·BASC-2, PRS-P)의 신뢰도와 타당도를 검증하여 영유아의 정신건강 선별검사로써의 유용성을 확인하고자 하였다. 이를 위해 어린이집 혹은 유치원에 재원 중인 영유아의 부모(N=479)를 대상으로 총 134문항으로 구성된 KㆍBASC-2, PRS-P를 실시하였다. 내적 합치도와 검사-재검사 신뢰도 분석을 통해 본 검사의 신뢰도를 확인하였으며, 요인분석과 문항반응이론 분석을 통해 내적 타당도를 검증하였다. 연구결과 본 검사의 종합척도와 소척도가 높은 내적 일치도를 보였으며 검사-재검사 신뢰도 또한 높게 나왔다. 요인분석 결과 K·BASC-2, PRS-P는 미국판 BASC-2, PRS-P의 3요인 구조가 적절히 지지되었다. 문항 수준에서의 타당도를 확인한 결과 대부분의 문항들의 변별도 및 범주 난이도가 적절한 것으로 확인되었으며, DSM 진단기준에 근거한 임상 증상 및 보호요인이 반영된 본 검사의 목적에 부합하는 문항 변별도 및 범주 난이도 간격을 보였다. 국내 영유아를 위한 다차원적 정신건강 선별검사로써의 K·BASC-2, PRS-P의 유용성, 본 연구의 장,단점 및 후속 연구를 위한 제언을 논의에 포함하였다. This study was to evaluate the reliability and validity of the Korean Version of the Behavior Assessment System for Child-2, Parent Rating Scales-Preschool Form(KㆍBASC-2, PRS-P) to verify its usefulness as a preschool mental health screening instrument. The 134-item K·BASC-2, PRS-P was administered to 479 Korean parents or caregivers with young children in preschool or kindergarten. Data analyses were conducted to obtain Cronbach"s α internal consistencies and test-retest reliabilities. A confirmatory factor analysis and the item response theory analysis were conducted to test internal validity. The results indicated that both internal consistencies on the subscale and composite scale level and test-retest reliabilities were high. The confirmatory factor analysis provided evidence for the three-factor structure in the Korean version as in the U.S. version. The item-level validity was high as indicated by the appropriate discrimination and the difficulty level for most items. The discrimination and skewness, as evidenced in the difficulty level, were consistent with the clinical symptoms and protective factors based on the DSM diagnostic criteria as expected. Discussion included relevant findings and practical implications, regarding the use of K·BASC-2, PRS-P as a multi-dimensional mental health screening instrument for Korean preschoolers. The strengths and limitations of the study as well as suggestions for future research are discussed.

Parental Roles in Children’s Sport Participation: Effects of Ethnicity and Immigration

Kyu-soo Chung,B. Christine Green 글로벌지식마케팅경영학회 2023 Journal of Global Sport Management Vol.8 No.4

Parents take on varied roles in their children’s sport, such as interpreters, provider, and model. These roles are enacted across settings, and impact the relationship families have with sport, and the value placed on sport vis-a-vis other contexts. This study looks at how Korean, Korean immigrant and Anglo-American parents carry out these roles, as the value parents place on their children’s education is controlled. One hundred forty-seven Korean parents in South Korea and 126 Korean immigrant and 112 Anglo-American parents in the U.S. provided answers to self-administered questionnaires regarding their supportive behavior children’s sport. The findings reveal the three differences in their encouragement, labor, and transportation provision; however, the findings reveal no difference in their modeling role. This study provides a theoretical understanding of how Korean immigrant parents affect their children’s sport participation, pointing to how social and cultural construction is reflected in their parenting and youth sport purchase decisions.

A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System

최상돈,장미숙,Tara Stuecker,Christine Chung,David A. Newcombe,Kasthuri Venkateswaran 한국유전체학회 2012 Genomics & informatics Vol.10 No.4

In this study, fosmid cloning strategies were used to assess the microbial populations in water from the International Space Station (ISS) drinking water system (henceforth referred to as Prebiocide and Tank A water samples). The goals of this study were: to compare the sensitivity of the fosmid cloning strategy with that of traditional culture-based and 16S rRNA-based approaches and to detect the widest possible spectrum of microbial populations during the water purification process. Initially, microbes could not be cultivated, and conventional PCR failed to amplify 16S rDNA fragments from these low biomass samples. Therefore, randomly primed rolling-circle amplification was used to amplify any DNA that might be present in the samples, followed by size selection by using pulsed-field gel electrophoresis. The amplified high-molecular- weight DNA from both samples was cloned into fosmid vectors. Several hundred clones were randomly selected for sequencing,followed by Blastn/Blastx searches. Sequences encoding specific genes from Burkholderia, a species abundant in the soil and groundwater, were found in both samples. Bradyrhizobium and Mesorhizobium, which belong to rhizobia, a large community of nitrogen fixers often found in association with plant roots, were present in the Prebiocide samples. Ralstonia,which is prevalent in soils with a high heavy metal content, was detected in the Tank A samples. The detection of many unidentified sequences suggests the presence of potentially novel microbial fingerprints. The bacterial diversity detected in this pilot study using a fosmid vector approach was higher than that detected by conventional 16S rRNA gene sequencing.

A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System

Choi, Sangdun,Chang, Mi Sook,Stuecker, Tara,Chung, Christine,Newcombe, David A.,Venkateswaran, Kasthuri Korea Genome Organization 2012 Genomics & informatics Vol.10 No.4

In this study, fosmid cloning strategies were used to assess the microbial populations in water from the International Space Station (ISS) drinking water system (henceforth referred to as Prebiocide and Tank A water samples). The goals of this study were: to compare the sensitivity of the fosmid cloning strategy with that of traditional culture-based and 16S rRNA-based approaches and to detect the widest possible spectrum of microbial populations during the water purification process. Initially, microbes could not be cultivated, and conventional PCR failed to amplify 16S rDNA fragments from these low biomass samples. Therefore, randomly primed rolling-circle amplification was used to amplify any DNA that might be present in the samples, followed by size selection by using pulsed-field gel electrophoresis. The amplified high-molecular- weight DNA from both samples was cloned into fosmid vectors. Several hundred clones were randomly selected for sequencing, followed by Blastn/Blastx searches. Sequences encoding specific genes from Burkholderia, a species abundant in the soil and groundwater, were found in both samples. Bradyrhizobium and Mesorhizobium, which belong to rhizobia, a large community of nitrogen fixers often found in association with plant roots, were present in the Prebiocide samples. Ralstonia, which is prevalent in soils with a high heavy metal content, was detected in the Tank A samples. The detection of many unidentified sequences suggests the presence of potentially novel microbial fingerprints. The bacterial diversity detected in this pilot study using a fosmid vector approach was higher than that detected by conventional 16S rRNA gene sequencing.

Retraction: A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System

Choi, Sangdun,Chang, Mi Sook,Stuecker, Tara,Chung, Christine,Newcombe, David A.,Venkateswaran, Kasthuri Korea Genome Organization 2013 Genomics & informatics Vol.11 No.2

Phosphorylation and Inactivation of Glycogen Synthase Kinase 3β (GSK3β) by Dual-specificity Tyrosine Phosphorylation-regulated Kinase 1A (Dyrk1A)

Song, Woo-Joo,Song, Eun-Ah Christine,Jung, Min-Su,Choi, Sun-Hee,Baik, Hyung-Hwan,Jin, Byung Kwan,Kim, Jeong Hee,Chung, Sul-Hee American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.4

<P>Glycogen synthase kinase 3 beta (GSK3 beta) participates in many cellular processes, and its dysregulation has been implicated in a wide range of diseases such as obesity, type 2 diabetes, cancer, and Alzheimer disease. Inactivation of GSK3 beta by phosphorylation at specific residues is a primary mechanism by which this constitutively active kinase is controlled. However, the regulatory mechanism of GSK3 beta is not fully understood. Dual-specificity tyrosine phosphorylation-regulated kinase 1A (Dyrk1A) has multiple biological functions that occur as the result of phosphorylation of diverse proteins that are involved in metabolism, synaptic function, and neurodegeneration. Here we show that GSK3 beta directly interacts with and is phosphorylated by Dyrk1A. Dyrk1A-mediated phosphorylation at the Thr(356) residue inhibits GSK3 beta activity. Dyrk1A transgenic (TG) mice are lean and resistant to diet-induced obesity because of reduced fat mass, which shows an inverse correlation with the effect of GSK3 beta on obesity. This result suggests a potential in vivo association between GSK3 beta and Dyrk1A regarding the mechanism underlying obesity. The level of Thr(P)(356)-GSK3 beta was higher in the white adipose tissue of Dyrk1A TG mice compared with control mice. GSK3 beta activity was differentially regulated by phosphorylation at different sites in adipose tissue depending on the type of diet the mice were fed. Furthermore, overexpression of Dyrk1A suppressed the expression of adipogenic proteins, including peroxisome proliferator-activated receptor gamma, in 3T3-L1 cells and in young Dyrk1A TG mice fed a chow diet. Taken together, these results reveal a novel regulatory mechanism for GSK3 beta activity and indicate that overexpression of Dyrk1A may contribute to the obesity-resistant phenotype through phosphorylation and inactivation of GSK3 beta.</P>

Single-cell RNA sequencing identifies distinct transcriptomic signatures between PMA/ionomycin- and αCD3/αCD28-activated primary human T cells

Jung Ho Lee,Brian H Lee,Soyoung Jeong,Christine Suh-Yun Joh,Hyo Jeong Nam,Hyun Seung Choi,Henry Sserwadda,Ji Won Oh,Chung-Gyu Park,Seon-Pil Jin,Hyun Je Kim Korea Genome Organization 2023 Genomics & informatics Vol.21 No.2

Immunologists have activated T cells in vitro using various stimulation methods, including phorbol myristate acetate (PMA)/ionomycin and αCD3/αCD28 agonistic antibodies. PMA stimulates protein kinase C, activating nuclear factor-κB, and ionomycin increases intracellular calcium levels, resulting in activation of nuclear factor of activated T cell. In contrast, αCD3/αCD28 agonistic antibodies activate T cells through ZAP-70, which phosphorylates linker for activation of T cell and SH2-domain-containing leukocyte protein of 76 kD. However, despite the use of these two different in vitro T cell activation methods for decades, the differential effects of chemical-based and antibody-based activation of primary human T cells have not yet been comprehensively described. Using single-cell RNA sequencing (scRNA-seq) technologies to analyze gene expression unbiasedly at the single-cell level, we compared the transcriptomic profiles of the non-physiological and physiological activation methods on human peripheral blood mononuclear cell-derived T cells from four independent donors. Remarkable transcriptomic differences in the expression of cytokines and their respective receptors were identified. We also identified activated CD4 T cell subsets (CD55⁺) enriched specifically by PMA/ionomycin activation. We believe this activated human T cell transcriptome atlas derived from two different activation methods will enhance our understanding, highlight the optimal use of these two in vitro T cell activation assays, and be applied as a reference standard when analyzing activated specific disease-originated T cells through scRNA-seq.

Differential diagnosis of breast masses in South Korean premenopausal women using diffuse optical spectroscopic imaging

Leproux, Anaï,s,Kim, You Me,Min, Jun Won,McLaren, Christine E.,Chen, Wen-Pin,O’Sullivan, Thomas D.,Lee, Seung-ha,Chung, Phil-Sang,Tromberg, Bruce J. SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS 2016 JOURNAL OF BIOMEDICAL OPTICS Vol.21 No.7

The MALATANG Survey: The <i>L</i>_GAS-<i>L</i>_IR Correlation on Sub-kiloparsec Scale in Six Nearby Star-forming Galaxies as Traced by HCN <i>J</i> = 4 → 3 and HCO⁺<i>J</i> = 4 → 3

Tan, Qing-Hua,Gao, Yu,Zhang, Zhi-Yu,Greve, Thomas R.,Jiang, Xue-Jian,Wilson, Christine D.,Yang, Chen-Tao,Bemis, Ashley,Chung, Aeree,Matsushita, Satoki,Shi, Yong,Ao, Yi-Ping,Brinks, Elias,Currie, Malco American Astronomical Society 2018 The Astrophysical journal Vol.860 No.2

<P>We present HCN J = 4 -> 3 and HCO+ J = 4 -> 3 maps of six nearby star-forming galaxies, NGC 253, NGC 1068, IC 342, M82, M83, and NGC 6946, obtained with the James Clerk Maxwell Telescope as part of the MALATANG survey. All galaxies were mapped in the central 2' x 2' region at 14 '' (FWHM) resolution (corresponding to linear scales of similar to 0.2-1.0 kpc). The L-IR-L'(dense) relation, where the dense gas is traced by the HCN J = 4 -> 3 and the HCO+ J = 4 -> 3 emission, measured in our sample of spatially resolved galaxies is found to follow the linear correlation established globally in galaxies within the scatter. We find that the luminosity ratio, L-IR/L'(dense), shows systematic variations with L-IR within individual spatially resolved galaxies, whereas the galaxy-integrated ratios vary little. A rising trend is also found between L-IR/L'(dense) ratio and the warm-dust temperature gauged by the 70 mu m/100 mu m flux ratio. We find that the luminosity ratios of IR/HCN (4-3) and IR/HCO+ (4-3), which can be taken as a proxy for the star formation efficiency (SFE) in the dense molecular gas (SFEdense), appear to be nearly independent of the dense gas fraction (f(dense)) for our sample of galaxies. The SFE of the total molecular gas (SFEmol) is found to increase substantially with f(dense) when combining our data with those on local (ultra) luminous infrared galaxies and high-z quasars. The mean L'(HCN(4-3))/L'(HCO+(4-3)) line ratio measured for the six targeted galaxies is 0.9 +/- 0.6. No significant correlation is found for the L'(HCN(4-3)) L'(HCO+(4-3)) ratio with the star formation rate as traced by L-IR, nor with the warm-dust temperature, for the different populations of galaxies.</P>