국내 다층지반의 지반물성치 및 토압에 관한 고찰

구자갑,김운학,최정호,유영화,정양규 한경대학교 2004 論文集 Vol.26 No.1

This paper study on soil properties and earth pressure at the domestic multi layer. Reliable predictions of the movement of earth retaining structures and the ground adjacent to the braced walls in urban excavation are often difficult due to many variable factors. Also, Unpredictable behavior of ground and retaining structure due to variation of the above factors may cause considerable damage to the adjacent structures, and cost many of human lives as a results of retaining wall failure. The earth pressure at the retaining walls was measured at total 13 numbers sites and Peck's empirical earth pressures adapted at the design stage. The geotechnical properties by this research was proposed as a preliminary design guide line in urban excavation where controling ground movement and safety assurance of adjacent structure. The earth pressure of rock masses were found to be 70.14% of Peck's earth pressure and the earth pressure of soil were found to be 44.4% of Peck's earth pressure. It is indicated that the design earth pressures of a retaining wall is

초등학교 수학과 학습지도에서 포트폴리오 평가의 적용과 그 효과 분석

손양자, 최창우 大邱敎育大學 科學敎育硏究所 2016 과학·수학교육연구 Vol.39 No.-

초등학교 5학년 학생을 대상으로 포트폴리오 평가를 실시하였으며, 실험반은 포트폴리오 자료에 들어갈 과제를 개발․제작하여 포트폴리오 서술식 채점기준에 의하여 평가를 실시하였다. 연구의 검사 도구로는 실험반과 비교반의 학업성취도 차이와 포트폴리오 평가 방법에 따른 효과를 분석하였고, 학업성취도는 문제해결력과 창의적 영역만을 비교하였다. 그리고 실험반의 사전․사후 수학의 정의적 영역 검사 및 포트폴리오를 마치고 설문조사한 내용과 면담을 분석하여 포트폴리오 평가가 수학의 정의적 영역에 어떤 영향을 미치는지를 검증하였다.

CD28Ig 및 CTLA4Ig 융합단백 생산 포유동물 세포주 수립에 관한 연구

유양석,정용훈,최용,임대철,조양자 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.2

CTLA-4, which is homologous to CD28, recognizes the same coutner-receptors B7.1 and B7.2. Although initial researches implied that these molecules may act to each other synergistically, recent data suggest that CTLA-4 may acts as a negative regulator of T cell activation. In this study human CD28 and CTLA-4 cDNAs were amplified from PHA-stimulated human peripheral blood lymphocytes mRNAs by using RT-PCR method. IgG1 Fc portion cDNA was amplified from human peripheral blood lymphocytes of patients with febrile illness and fused to C"-terminal parts of CD28 and CTLA-4 extracellular domains. The fusion constructs were subcloned into pCI-neo vector and then transfected into Sp-2/0murine myeloma cell. Stable cell lines were established by geneticin selection and cloning. In the sequence of CTLA-4 cDNA from 3 different healthy persons it was found that 2 sites of bases were different from that published in original paper and submitted to genbank database. The 49th and 331st bases of open reading frame were changed from adenine to guanine and from guanine to adenine respectively. It was confirmed that the 17th and 111th amino acid of human CTLA-4 were alanine and threonine respectively, 5 different human CTLA-4 clones with serial N'-terminal deletion were constructed. CTLA4/L_1 was intact CTLA-4 gene of native form without any form of mutation. CTLA4/L_2 was a mutatn with deletion in gene coding N'-termial 6 amino acids. CTLA4/L_3 was a mutant with deletion in gene coding N'-termial 11 amino acids. CTLA4/L_4 was a mutant with deletion in gene coding N'-termial 16 amino acids. CTLA4/L_5 was a mutant with deletion in gene coding N'-termial 22 amino acids. Level of surface expression of CTLA4Ig on L_1, L_2, L_3, L_4 and L_5 transfectant cell lines were 5%, 3%, -1.5%, 8%, and 6% respectively. These 2 fusion proteins, CD28Ig and CTLA4Ig, would be useful tools for further researches on role of the costimulations in T cell activation.

장애학생을 위한 특수체육학급 설치에 관한 조사연구

홍양자,최인옥 한국특수체육학회 2003 한국특수체육학회지 Vol.11 No.2

The purpose of this study was to determine the current status of the establishment adapted physical education class for students with disabilities who cannot participate normal physical class and to give the research data by surveying the attitudes of the administrators, teachers and parents about adapted physical education class. the survey was executed the by teacher, normal teacher, administrator, and parents to research their attitudes towards the establishment of adapted physical education class. The finding are as follows: First in the case of a school which has no special class, the result of the survey was affirmative about the establishment of a special class. This result means that the interest and concern for the students with disabilities is higher. Second, concerning the necessity of the adapted physical education class, the attitude of the administrator, teacher and parents were very affirmative. Third, the physical teacher thought the students with disabilities to be insufficient in activity ability but to could do better through adapted physical education. Consequently, in school and home, positive concern and consideration help students with disabilities to grow up like normal children. First of all, adapted physical class should be established to develope physical education of special class.

혈액관리학과 교육과정 개발

조갑철,최양자,신기수,손선영 적십자간호전문대학 2000 적십자간호전문대학논문집 Vol.22 No.-

마우스 Myeloma Cell P3-X63-Ag8.653 및 T Cell Hybridome DO11.10에서의 Programmed Cell Death 유발 특성 비교

임석원,정용훈,최용,한승룡,조양자 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.2

This study was aimed to prove that the death of HGPRT myeloma V653 by aminopterin treatment is due to PCD. This PCD was also compared with a relatively well-defined dexamethasone-induced PCD of T-cell hybridoma DO11.10. And these PCD systems were further characterized by inhibiting protein synthesis with cycloheximide, inhibiting mRNA synthesis with actinomycin-D, increasing intracellular calcium with calcium ionophore A23187. Induction of c-myc, an universal apoptosis gene, was also compared between these 2 systems. Viability of V653 cell was decreased by aminopterin-treatment in a dose-dependent manner. After treatment of aminopterin, this decrease of viability began in 6 hours and was accelerated in 12-18 hours. DNA fragmentation of V653 was detectable at 3 hours of incubation and peaked in 12-18 hours. Patterns of decrease in cell viability and increase in DNA fragmentation of dexamethasone-treated DO11.10 cells was similar to that of aminopterin-treated V653 cell, but the progress was much slower. The decrease in cell viability and increase in DNA fragmentation of aminopterin-treated V653 cells were inhibited by protein synthesis inhibitor cycloheximide, and RNA synthesis inhibitor actinomycin-D. In contrast the decrease in cell viability and increase in DNA fragmentation of dexamethasone-treated DO11.10 cell were accelerated by cycloheximide and actinomycin-D additions. Ca^2+ ionophore A23187 addition to aminopterin-treated V653 cells and dexamethasone-treated DO11.10 accelerated the decrease in cell viability and the increase in DNA fragmentation of both cells. In northern blot analysis, induction of c-myc gene was observed in aminopterin-treated V653 cell reached peak at 2hours and thern decreased drastically. Induction of c-myc gene in dexamethasone-treated DO11.10 cells was much slower and the fold of gene induction was much lower than that of aminopterin-treated V653 cell. Ultrastructural changes during the death process were observed by using electron microscope. Dilatation of rough endoplasmic reticulum(RER) and detachment of ribosomes were the earliest ultrastructural changes, which were observed in 30 minutes after death induction. The results of this study suggest that aminopterin-induced selective elimination of fusion partner V653 myeloma cell is due to PCD, and that this process requires de novo protein and RNA synthesis in which a Ca^2+ -mediated signal transduction pathway is involved.

T 세포활성항원 CTLA-4의 기능에 관한 연구 : T 세포에서 표적세포 살해능과 CTLA-4 발현과의 연관성 Target Cytotoxicity of T Cell Correlates with CTLA-4 Production

노만택,조양자,김용식,최용,조보현,최장원,정용훈 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

CTLA-4, a T cell activation antigen and a homologue of CD28, was originally identified as a gene by a series of reverse genetic approaches. While CD28 molecule has been characterized well as a stimulator of T-cell function via enhanced lymphokines production and stablilization of those mRNA, most of the functions of CTLA-4 remain unknown. It has been widely accepted that CTLA-4 functions as an immune suppressor which is down-regualting the function of CD28. We previously showed that 34 KD form of this antigen mainly expressed CD8+ subset, a cytotoxic or suppressor, of activated peripheral blood lymphocyte. Based on our previous finding this study was conducted to further characterize immunological function of CTLA-4 especially in terms of involvement of this molecule in T-cell effector function mediating target cell cytotoxicity. 4 human T cell clones with different target cytotoxicities were employed in this study. NBL46 (CD4+) and NBL77 (CD8+) were cytotoxic and NBL32 (CD4+) and NBL58 (CD 8+) were non-cytotoxic to target LBF cell in target cell chromium release assay. And in Western blot assay 34 kD antigen was detected in NBL46 (CD4+) and NBL77 (CD8+) clones but not in NBL32 (CD4+) and NBL58 (CD8+) clones. It was summarized that expression of the CTLA-4 antigen was associated with cytotoxicity but not with subset phenotypes of T cells. In conclusion CD8+ T subset of PHA-stimulated PBL was major and only CTLA-4 producer and this molecule was induced during mid to late stage of T cell activation. The cytotoxicity of T cell clones to its target cell was directly correlated with its CTLA-4 production and vice versa. And it was highly suggested that primary function of CTLA-4 may involved in T cell effector function which mediates target cell killing.

Benson's Group Additivity Method를 이용한 염화다환방향족 화합물의 열역학적물성치 평가와 생성반응경로에 관한 연구

임지선,박자영,원양수,최성필 嶺南大學校 環境問題硏究所 1995 環境硏究 Vol.15 No.1

도시폐기물 소각시설에서 다이옥신류는 chlorobenzene, chlorophenols, PCBs등의 염소성분이 포함된 방향족화합물과 함께 폐기물의 소각처리시 불완전 연소로 인해 주로 생성된다. 이들 다이옥신 생성에 대한 반응메카니즘의 예측에 필요한 다이옥신을 형성하는 전구물질인 분자 및 radical의 정확한 열역학적 물성치를 평가하기 위해 Benson's group additivity method에서는 고려되지 않는 Cl, F, OH, CH3등과 같은 2개 이상의 치환기가 결합된 방향족화합물에 대한 computer code인 THERM을 이용하여 엔탈피(Hf), 엔트로피(St), 열용량(Cp)을 평가하였다. 또한 이와 같은 열역학적 물성치 평가법의 신뢰성을 검토하기 위해 다양한 방향족화합물에 대한 실험적으로 측정된 문헌치와 평가치와의 절대오차는 para-cresol의 0.02kcal/mole에서 hexamethylbenzene의 3.46kcal/mole로 좁은 범위를 나타내었다. 또한 다이옥신을 생성하는 전구물질들의 열역학적 물성치를 THERM을 이용해 간접적으로 평가함으로써 다이옥신을 형성하는 주요 생성반응 경로에 대해 살펴본 결과는 다이옥신을 형성하는 기초반응(elementary reaction)중 욤소수가 많은 반응물질일수록 반응이 더 잘 일어남을 알수 있었다. Chlorinated dioxins are often observed in effluent from oxidation or incineration such as chlorobenzenes, chlorophenols and PCBs. It would be of significant value to have an accurate and fundamental understanding of the mechanism for dioxin formation, in addition to the knowledge of dioxin thermodynamic properties, in order to assist in the design of stategies to effectively control or reduce emissions of these compounds. Very little has been published on the thermodynamic properties and elementary reactions which lead to formation of dioxins. Benson group additivity method, when used for the estimation of thermodynamic properties of molecules, does not account for all interactions between functional groups or atoms on aromatics, such as chlorines, hydroxyl or ether linkages. Therefore thermodynamic properties of dioxins are estimated by THERM based on Benson's group additivity method corrected by interactions group. Absolute errors between our predictions and literature values range from a low of 0.02 kcal/mole for paracresol to a high 3.46kcal/mole for hexamethylbenzene. We use these thermodynamic calculations to show the feasibility of several elementary reaction pathways leading to dioxin formation, where the presence of chlorine acts to enhance or lower the Gibbs free energy of reactions.

A New Six-rowed and Covered Barley(Hordeum vulgare L.) Cultivar, “Hyegang” with BaYMV Resistant, High Yield and Good Malt Quality

Yang-Kil Kim,Mi-Ja Lee,Jong-Nae Hyun,Seong-Bum Baek,Jong-Chul Park,Jai-Hyun Jeung,Hong-Jib Choi,Su-Hyun Cho,Mi-Jung Kim,Hyoung-Ho Park,Jae-Seong Choi,Tae-Su Kim,Kee-Jong, kim,Ki-Hun Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Improvement of winter hardiness, diseases resistance and good quality have been recently received more attention by covered barley(Hordeum vulgare L.) breeders than ever in Korea. “Hyegang”, a new covered barley cultivar with similar maturing and high yield was developed by the National Institute of Crop Science, RDA in 2011. It was derived from the cross between “Milyang87/NWX-GB-G 2” and “Milyang87/Chalbori” with good quality. The initial cross was done in 2000 and the selected line, “Iksan445” (YB4625-B-B-6-2), showed high yield and good quality characteristics under yield trial test in 2008. It was cultivated three times for three years (2009~2011) in the four locations of regional yield trial (RYT) in korea and was investigated about agronomic and quality characteristics and released as “Hyegang”. The heading date was May 4 in upland and April 26 in paddy field which was 1 and 0 day lateness respectively than that of check cultivar, “Olbori”. The culm length was 77 cm which was 6 cm shorter than that with check cultivar. It showed the spike length of 4.7 cm, 800 spikes / m2, 64 grains / spike and 25.1g for 1,000 grains weight. It showed similar maturing and stronger resistance to barley yellow mosaic virus (BaYMV) compare to check cultivar. It showed higher diastatic power 216DP than that of check cultivar, ‘Olbori’. Average yield of “Hyegang” in the RYT was 4.61 MT/ha in upland and 4.38 MT/ha in paddy field, espectively.

Cytocompatible <i>in situ</i> cross-linking of degradable LbL films based on thiol–exchange reaction

Yang, Sung Ho,Choi, Jinsu,Palanikumar, L.,Choi, Eun Seong,Lee, Juno,Kim, Juan,Choi, Insung S.,Ryu, Ja-Hyoung Royal Society of Chemistry 2015 Chemical Science Vol.6 No.8

<▼1><P>A highly cytocompatible LbL process was developed, based on the thiol–exchange reaction, for forming <I>in situ</I> cross-linked and degradable films under physiologically mild conditions.</P></▼1><▼2><P>Formation of both mechanically durable and programmably degradable layer-by-layer (LbL) films in a biocompatible fashion has potential applications in cell therapy, tissue engineering, and drug-delivery systems, where the films are interfaced with living cells. In this work, we developed a simple but versatile method for generating <I>in situ</I> cross-linked and responsively degradable LbL films, based on the thiol–exchange reaction, under highly cytocompatible conditions (aqueous solution at pH 7.4 and room temperature). The cytocompatibility of the processes was confirmed by coating individual yeast cells with the cross-linked LbL films and breaking the films on demand, while maintaining the cell viability. In addition, the processes were applied to the controlled release of an anticancer drug in the HeLa cells.</P></▼2>