Positive Association of TEAD1 With Schizophrenia in a Northeast Chinese Han Population

Yang Sun,Lin Wen,Yi-Yang Luo,Wen-Juan Hu,Hui-Wen Ren,Ye Lv,Cong Zhang,Ping Gao,Li-Na Xuan,Guan-Yu Wang,Cheng-Jie Li,Zhi-Xin Xiang,Zhi-Lin Luan 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.12

Objective Schizophrenia is a complex and devastating psychiatric disorder with a strong genetic background. However, much uncertainty still exists about the role of genetic susceptibility in the pathophysiology of schizophrenia. TEA domain transcription factor 1 (TEAD1) is a transcription factor associated with neurodevelopment and has modulating effects on various nervous system diseases. In the current study, we performed a case–control association study in a Northeast Chinese Han population to explore the characteristics of pathogenic <i>TEAD1</i> polymorphisms and potential association with schizophrenia.Methods We recruited a total of 721 schizophrenia patients and 1,195 healthy controls in this study. The 9 single nucleotide polymorphisms (SNPs) in the gene region of <i>TEAD1</i> were selected and genotyped.Results The genetic association analyses showed that five SNPs (rs12289262, rs6485989, rs4415740, rs7113256, and rs1866709) were significantly different between schizophrenia patients and healthy controls in allele or/and genotype frequencies. After Bonferroni correction, the association of three SNPs (rs4415740, rs7113256, and rs1866709) with schizophrenia were still evident. Haplotype analysis revealed that two strong linkage disequilibrium blocks (rs6485989-rs4415740-rs7113256 and rs16911710-rs12364619-rs1866709) were globally associated with schizophrenia. Four haplotypes (C-C-C and T-T-T, rs6485989-rs4415740-rs7113256; G-T-A and G-T-G, rs16911710-rs12364619-rs1866709) were significantly different between schizophrenia patients and healthy controls.Conclusion The current findings indicated that the human <i>TEAD1</i> gene has a genetic association with schizophrenia in the Chinese Han population and may act as a susceptibility gene for schizophrenia.

Banana Resistant Starch and Its Effects on Constipation Model Mice

Juan Wang,Ji Hong Huang,Yan Feng Cheng,Gong Ming Yang 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.8

Banana resistant starch (BRS) was extracted to investigate the structural properties of BRS, its effects on the gastrointestinal transit, and dejecta of normal and experimentally constipated mice. The mouse constipation model was induced by diphenoxylate administration. The BRS administered mice were divided into three groups and gavaged with 1.0, 2.0, or 4.0 g/kg body weightBRS per day. The small intestinal movement, time of the first black dejecta, dejecta granules,weight and theirmoisture content, body weight, and food intake of mice were studied. Results showed that the BRS particles were oval and spindly and some light cracks and pitswere in the surface. The degree of crystallinity of BRSwas 23.13%; the main diffraction peaks were at 2h 15.14, 17.38, 20.08, and 22.51. The degree of polymerization of BRS was 81.16 and the number-average molecular weight was 13147.92Da, as determined by the reducing terminal method. In animal experiments, BRS at the dose of 4.0 g/kg body weight per daywas able to increase the gastrointestinal propulsive rate, and BRS at the doses of 2.0 and 4.0 g/kg bodyweight per daywas found to shorten the start time of defecation by observing the first black dejecta exhaust. However, there were no influences of BRS on the dejecta moisture content, the dejecta granules and their weight, body weight, or daily food intake in mice. BRS was effective in accelerating the movement of the small intestine and in shortening the start time of defecation, but did not impact body weight and food intake. Therefore, BRS had the potential to be useful for improving intestinal motility during constipation.

Structure Analysis and Laxative Effects of Oligosaccharides Isolated from Bananas

Juan Wang,Hui Hua Huang,Yan Feng Cheng,Gong Ming Yang 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.10

Banana oligosaccharides (BOS) were extracted with water, and then separated and purified using column chromatography. Gel penetration chromatography was used to determine the molecular weights. Thin layer chromatogram and capillary electrophoresis were employed to analyze the monosaccharide composition. The indican bond and structure of the BOS molecule were determined using Fourier transform infrared spectroscopy and nuclear magnetic resonance. Results showed that BOS were probably composed of eight β-D-pyran glucose units linked with 1→6 indican bonds. The laxative effects of BOS were investigated in mice using the method described in “Handbook of Technical Standards for Testing and Assessment of Health Food in China.” The length of the small intestine over which a carbon suspension solution advanced in mice treated with low-, middle-, and high-dose BOS was significantly greater than that in the model group, suggesting that BOS are effective in accelerating the movement of the small intestine.

Two New Species of Placolecis (Lichenized Ascomycota) from China

( An Cheng Yin ),( Xin Yu Wang ),( Dong Liu ),( Yan Yun Zhang ),( Mei Xia Yang ),( Li Juan Li ),( Li Song Wang ) 한국균학회 2019 Mycobiology Vol.47 No.4

Two new species of the lichen genus Placolecis are discovered in China, namely P. kunmingensis An. C. Yin & Li S. Wang and P. sublaevis An. C. Yin & Li S. Wang. The new combination P. loekoesiana (S.Y. Kondr., Farkas, J.J. Woo & Hur) An. C. Yin is proposed. Placolecis kunmingensis is characterized by having simple, spherical or ellipsoid, hyaline spores, and pear-shaped pycnidia; while P. sublaevis can be distinguished by its thallus forming larger aggregations with slightly flattened lobes at the thallus margin, and urn-shaped pycnidia. Descriptions, a phylogenetic tree and a key are provided for all the known Placolecis species in China.

Synthesis, Characterization and pH-Responsive Self-Assembly Behavior of Amphiphilic Multiarm Star Triblock Copolymers Based on PCL, PDEAEMA, and PEG

You Qiang Yang,Wen Jing Lin,Li Juan Zhang,Cheng Zhi Cai,Wei Jiang,Xin Dong Guo,Yu Qian 한국고분자학회 2013 Macromolecular Research Vol.21 No.9

A series of amphiphilic 4- and 6-armed star triblock copolymers based on poly(ε-caprolactone) (PCL),poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA), and poly(poly(ethylene glycol) methyl ether methacrylate)(PPEGMA) were designed and synthesized by a combination of ring opening polymerization (ROP) and continuous activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP). The continuous ARGET ATRP of DEAEMA and PEGMA was in situ monitored by react infrared spectroscopy (ReactIR) and showed good first-order kinetic characteristics. The molecular weights and chemical structures of the copolymers and their precursors were confirmed by gel permeation chromatography (GPC) and 1H NMR. The critical micelle concentration (CMC) values of the star copolymers in aqueous solution were extremely low (2.2-4.0 mg/L), depending on the architecture of the copolymers. The pH-responsive self-assembly behavior of the star copolymers in aqueous solution was investigated by a combination of dynamic light scattering (DLS), UV-vis spectrometry and scanning electron microscopy (SEM). When the pH values decreased from 10 to 3, no obvious fluctuation of the visible light transmittance of the micelle solutions was observed for lower polymer concentrations of 0.1 and 1 mg/mL, while sharp increase occurred at higher concentration of 10 mg/mL. The hydrodynamic diameters (Dh) of the micelle solutions appeared slight increase with the increase of concentration, and increased rapidly as the pH decreased from 10 to 4 followed by a slight decrease at pH 3. The effects of pH value on the zeta potentials exhibited almost the same tendency with the Dh. This may due to the fact that the protonation of tertiary amine groups in DEAEMA can induce the swelling of micelles. The PCL and PDEAEMA contents and the topological structures (4-or 6-arm) showed significant influences on the pH-sensitivity of the micelles. Overall, the results demonstrated that the structures and pH-sensitivity of these amphiphilic copolymers could be well-controlled and their self-assembled micelles are promising carriers for delivery of anticancer hydrophobic drugs.

Study on Interaction of the Diversity of Paddy field Spider Community and Its Objective Pests Occurring Degree

Wnag Zhi,Cheng Juan-yang,Yan Heng-mei,Joo-Pil Kim 한국거미연구소 2002 한국거미 Vol.18 No.1

Detection of rare point mutation via allele-specific amplification in emulsion PCR

Chang Ming Cheng,Yin Zhou,Chao Yang,Juan Chen,Jie Wang,Jie Zhang,Guo Ping Zhao 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.5

Potassium ions as a kinetic controller in ionic double layers for hysteresis-free perovskite solar cells

Kim, Seul-Gi,Li, Cheng,Guerrero, Antonio,Yang, June-Mo,Zhong, Yu,Bisquert, Juan,Huettner, Sven,Park, Nam-Gyu The Royal Society of Chemistry 2019 Journal of materials chemistry. A, Materials for e Vol.7 No.32

<P>Since ion migration and interaction with external contacts has been regarded as one of the origins for photocurrent density (<I>J</I>)-voltage (<I>V</I>) hysteresis and phase segregation in perovskite solar cells (PSCs) under operational condition, control of ionic movement in organic-inorganic halide perovskites presents a big challenge for achieving hysteresis-free and stable PSCs. As a universal method, potassium doping into bulk perovskite films to minimize or eliminate the hysteresis was proposed. Here, we report direct observation of moderately retarded ion migration in K<SUP>+</SUP>-doped (FAPbI3)0.875(CsPbBr3)0.125 perovskite by <I>in situ</I> photoluminescence (PL) imaging. However, more impressive is the effect on the kinetics for generation of the ionic double layer in the vicinity of the contacts as it is reduced by two orders of magnitude on the time scale when devices are doped with K<SUP>+</SUP> as detected by impedance spectroscopy. A significantly reduced hysteresis in the K<SUP>+</SUP>-doped perovskite is responsible for more prolonged stability exhibiting ∼96% of initial power conversion efficiency (PCE) after 22 days than relatively short-lived perovskites undoped with K<SUP>+</SUP> ions. This work highlights the clear correlation of ion migration and a fast generation of the double layer close to the contacts with severe hysteresis and long-term instability in PSCs and the importance of K<SUP>+</SUP> ions in reducing the kinetics affecting the ionic attachment to the contact surface.</P>

Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity

Zhang, Bing-Huo,Cheng, Juan,Chen, Wei,Li, Han-Quan,Yang, Jian-Yuan,Park, Dong-Jin,Kim, Chang-Jin,Shen, Rui,Duan, Yan-Qin,Li, Wen-Jun Nature Publishing Group 2015 The Journal of Antibiotics Vol. No.

<P>Strain JXJ 0135(T), an anti-cyanobacterial actinomycete, was isolated from a soil sample collected from Lushan Mountain, south China, and identified by using polyphasic approach. Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that strain JXJ 0135(T) belongs to the genus Streptomyces and exhibited distinct subclade and also highest similarity (98.6%) to Streptomyces scopuliridis RB72(T). The strain developed well-branched substrate and aerial mycelia, and produced spiral spore chains. Spores were elliptical and the spore surface was smooth. The strain contained LL-diaminopimelic acid with whole-cell sugars of mannose, rhamnose, glucose and galactose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphotidylinositol dimannoside, an unidentified aminophospholipid and an unknown phospholipid. The menaquinones were MK-9(H6) and MK-9(H8). The major components of the fatty acids were anteiso-C15:0, iso-C16:0, anteiso-C17:0, iso-C15:0, C16:0, iso-C17:0 and iso-C14:0. The G+C content was 69.3?mol%. The DNA-DNA hybridization value between JXJ 0135(T) and S. scopuliridis RB72(T) was 41.21.4%. On the basis of the polyphasic data, strain JXJ 0135(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lushanensis sp. nov. is proposed. The type strain is JXJ 0135(T) (=DSM 42121(T)=JCM 19628(T)=KCTC 29261(T)=KACC 17834(T)=NRRL B-24994(T)).</P>

Gestational Diabetes Mellitus and Subsequent Cancer Risk: Shared Risk Factors, Causality or Confounding?

Xie, Shao-Hua,Yang, Shu-Juan,Chen, Juan,Cheng, Jin-Quan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24