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Chaojie Chong,Yaqi Wu,Zhewen Dong,Zixian Wang,Shumei Zhong,Haokun Chen,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was to investigate the ameliorative effect of GFPS on clinical symptoms in letrozole(1 mg/kg)-induced PCOS rats. The female SD rats were classified into 4 groups as follows: the normal group (gavaged with saline), the PCOS group(ingested letrozole daily for 21 days). Normal group and metformin group gavaged with letrozole followed by metformin (265 mg/kg) and GFPS(600 mg/kg), respectively. All rats were weighed every 3 days until the end of the experiment. The ovaries, perirenal, abdomen and periuterine fat were removed and the relative weights (g/bw×100) were calculated. Serum levels of FSH, LH, E2, T, TG, TC, LDL-C were measured by ELISA and calculated the LH/FSH ratio. GFPS reduced follicle number and increases granulosa cell layer thickness and luteal number. GFPS also reduced the increase of body weight, ovarian and visceral fat weight in PCOS rats (P<0.01). In addition, GFPS reduced the serum levels of T, LH/FSH, TC, TG, LDL-C and LH, and elevated the serum levels of E2 and FSH in PCOS rats. In conclusion, GFPS can improve follicular development and restore body weight, ovarian weight, serum hormone levels and lipid profile in PCOS rats.
Huiyan Zhou,Penghang Zhang,Jiang Luo,Xueying Liu,Shuangxi Fan,Chaojie Liu,Yingyan Han 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1
Head lettuce originating near the Mediterranean coast was introduced to China in the fi fth century. It is diffi cult to identifydiff erent varieties of head lettuce due to their close genetic relationships. Simple sequence repeats (SSR) technology is apractical method that has been widely used in variety identifi cation, fi ngerprint construction and genetic diversity analysis. In this paper, 23 pairs of SSR primers were selected to identify and analyse 73 varieties of head lettuce. The results identified a total of 117 mutated alleles detected in 23 loci, with the number of each loci ranging from 2 to 11, with an averageof 5.1 mutated alleles per locus. Additionally, 152 genotypes were detected in 23 loci, with an average of 6.6 genotypes perlocus derived from a range of 2–13. The polymorphism information content ranged from 0.071 to 0.702, with an average of0.499. The selected primer pairs could clearly refl ect the genotypic diversity level of the 73 head lettuce varieties tested, andcan be used in the detection and analysis of the head lettuce varieties. The results of our sequence analysis also showed thatthese 73 varieties of head lettuce could be eff ectively identifi ed by a combination of 19 primer pairs even though the geneticsimilarity among the 73 varieties ranged from 0.647 to 0.991, with an average of 0.798. Based on a capillary electrophoresisplatform, a DNA fi ngerprinting database of 73 head lettuce varieties was established.
Yaqi Wu,Chaojie Chong,Zhewen Dong,Zixian Wang,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To explore the ameliorative effect of Phellinus linteus polysaccharide(PLP) on letrozole-induced PCOS rats. A total of 32 female SD rats were randomly divided into normal group, PCOS group, metformin group and PLP group, and weighed every three days. The estrous cycle of rats was observed by vaginal smear ten days before sacrifice. The estrous cycle of PCOS rats was disordered, the ovaries showed polycystic changes and the body weight and the relative weight of the ovaries increased (P<0.05). The estrous cycle, polycystic ovary, body weight and relative weight of the ovaries in PLP group and Metformin group recovered significantly (P<0.05). The serum levels of TC, TG, LDL-C, HDL-C, FINS, T, LH, E2 and FSH were detected by ELISA kits. After treatment, the serum levels of TC, TG, LDL-C, FINS, T, LH and LH/FSH in the PLP group and Metformin group were lower than PCOS group (P<0.05). In addition, the serum levels of E2, FSH and HDL-C were increased (P<0.05) in the PLP group and Metformin group. The results showed that Phellinus linteus polysaccharides can ameliorate glucose and lipid metabolism, polycystic ovary and regulate the levels of sex hormones in PCOS rats.
Zhiqiang Liu,Chaojie Chong,Zhewen Dong,Yaqi Wu,Wanying Li,Haokun Chen,Shumei Zhong,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effect of ACP on letrozole-induced PCOS rats. All rats were divided into normal groups, PCOS group, metformin group and ACP group. The body weight (BW) was recorded every three days, and the final body weight as well as ovarian weight were recorded. Serum levels of TC, TG, HDL-C, LDL-C and E2, T, FSH and LH were measured by ELISA assay, and the LH/FSH ratio was calculated. The results showed that ACP decreased the BW and the ratio of ovarian to BW. Compared with the normal group, the serum levels of T, LH, LH/FSH, TG, TC, LDL-C increased, and the levels of FSH, E2, HDL-C decreased in PCOS group. ACP effectively improves the serum levels of FSH, LH/FSH, TG, TC, LDL-C and other related factors. The results of H&E staining of ovarian tissues showed that the ACP group showed different degrees of improvement in the reduced number of corpus luteum, thinning of granulosa cell layer, follicular capsular dilatation and follicular atresia. ACP not only reduces BW and the ratio of ovarian to BW, but also it can elevate the level of FSH and reduce LH/FSH, TG, TC and HDL-C levels. Furthermore, ACP can protect ovarian tissue to some extent.
Vibration control performance of particle tuned mass inerter system
Zheng Lu,Deyu Yan,Chaojie Zhou,Ruifu Zhang 국제구조공학회 2024 Structural Engineering and Mechanics, An Int'l Jou Vol.89 No.4
To improve the vibration control performance and applicability of traditional particle tuned mass damper (PTMD) and realize the significant characteristic of lightweight design, this study proposes a novel particle tuned mass inerter system (PTMIS) by introducing inerter system (IS) to the PTMD. In the study, the motion equation of single degree of freedom (SDOF) structure attached with PTMIS is established first, then the variation law of the system’s vibration reduction performance (VRP) is discussed through parameter analysis, and it is compared with the PTMD to analyze its VRP advantages. Finally, its vibration reduction (VR) mechanism from the perspective of core control force and energy analysis is explored, and its cavity relative displacement from the application perspective is analyzed. The results show that the PTMIS can remarkably improve the vibration control effectiveness of the PTMD. The reason is that the inerter can store energy and transfer the energy to the cavity and particles, which further stimulates the interaction between the two parts, thereby improving the nonlinear energy consumption effectiveness. Also, the IS can amplify the damping element’s energy dissipation efficiency. In addition, the PTMIS can effectively reduce the working stroke of the PTMD, and through the analysis of the lightweight characteristics of the PTMIS, it is found that its lightweight advantage can reach nearly 100%.
Huan Lan,Zhewen Dong,Yaqi Wu,Chaojie Chong,Zhiqiang Liu,Wanying Li,Zixian Wang,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study investigated the antifibrotic effects of SA on ovarian fibrosis in letrozole-induced PCOS in rats. SA treatment restored letrozole-induced alterations in the body weight (BW), relative weights of the ovaries, and visceral adipose tissues. Histological observation showed that SA reduced the number of atretic follicles, cystic follicles, and fibrosis of the ovaries in PCOS rats. SA treatment also modulated the serum levels of sex hormones, which decreased the luteinizing hormone (LH), and testosterone (T) and increased the follicle-stimulating hormone (FSH) in PCOS rats. Administration of SA reduced letrozole-induced metabolic dysfunction by decreasing the serum levels of triglyceride (TG) and total cholesterol (TC) and increasing the levels of high-density lipoprotein cholesterol (HDL-C) in PCOS rats. Finally, SA treatment appeared to increase the activity of PPAR-γ, reduce the activation of the TGF-β1/Smad pathway, and reduce the collagen I, α-SMA and CTGF levels in the ovaries of the PCOS rats. Our results demonstrated that SA significantly ameliorated ovarian fibrosis and abolic disturbances in the letrozole-induced PCOS rats.
Huan Lan,Zhewen Dong,Yaqi Wu,Chaojie Chong,Zhiqiang Liu,Wanying Li,Zixian Wang,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In this study, we investigated the antifibrotic effects of Rha on letrozole-induced PCOS in rats. Rha treatment restored letrozole-induced alterations in the body weight (BW), relative weights of the ovaries, and relative weights of uterine and visceral adipose tissues. Histological observation showed that Rha reduced the number of atretic follicles, cystic follicles, and fibrosis of the ovaries in the PCOS rats. Administration of Rha reduced letrozole-induced metabolic dysfunction by decreasing the serum levels of TG and TC and increasing the levels of HDL-C in the PCOS rats. Rha treatment also modulated the serum levels of sex hormones, which decreased the LH, E2 and T and increased the FSH in PCOS rats. Finally, Rha treatment appeared to increase the activity of PPAR-γ, reduce the activation of the TGF-β1/Smad pathway, and reduce the collagen I, α-SMA and CTGF levels in the ovaries of the PCOS rats. Our results demonstrated that Rha significantly ameliorated metabolic disturbances and ovarian fibrosis in the letrozole-induced PCOS rats. Rha may be an effective compound in preventing ovarian fibrosis in the future.