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표고버섯 균사체 배양기간에 따른 PDB 배지 내 유리 아미노산 변화
오태석 ( Tae-seok Oh ),박윤진 ( Youn-jin Park ),김태권 ( Tae-kwon Kim ),안승원 ( Seoung-won Ann ),김창호 ( Chang-ho Kim ),조용구 ( Yong-koo Cho ),장명준 ( Myoung-jun Jang ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.4
This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of 9,889 ± 3 μg/L to 12,909 ± 2 μg/L at 45 days and 29,256 ± 4 μg/L at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to 83,751 ± 2 μg/L after 45 days from its initial value of 161,787 ± 1 μg/L and to 79,055 ± 7 μg/L at 90 days.
Chloride Transport in OPC Concrete Subjected to the Freeze and Thaw Damage
Ann, Ki Yong,Kim, Min Jae,Hwang, Jun Pil,Cho, Chang-geun,Kim, Ki Hwan Hindawi Limited 2017 Advances In Materials Science And Engineering Vol.2017 No.1
<P>To predict the durability of a concrete structure under the coupling degradation consisting of the frosting and chloride attack, microstructural analysis of the concrete pore structure should be accompanied. In this study, the correlation between the pore structure and chloride migration for OPC concrete was evaluated at the different cement content in the concrete mix accounting for 300, 350, and 400 kg/m<SUP>3</SUP> at 0.45 of a free water cement ratio. The influence of frosting damage on the rate of chloride transport was assessed by testing with concrete specimens subjected to a rapid freezing and thawing cyclic environment. As a result, it was found that chloride transport was accelerated by frost damage, which was more influential at the lower cement content. The microscopic examination of the pore structure showed that the freezing environment increased the volume of the large capillary pore in the concrete matrix.</P>
장준형,안재범 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4
Sour liquid 발효가 식빵의 제조조건과 품질에 미치는 영향을 알아 보았다. Lactobacillus brevis와 Lactobacillus plamtarum을 혼합배양하면 sour dough bread 사용 유산균중 산생성력이 가장 높았고 pH도 낮았다. Sour liquid 발효 제조시 제빵의 제조조건에 가장 적합한 배양조건, 배양온도, 시간 및 배지의 조성에 대해 실험하였다. L. brevis와 L. plantarum의 배양은 30℃에서 24시간 하였다. 배지조성중 글루코오스를 2% 첨가하고 영양원으로서 yeast extract 0.4%를 첨가한 것의 결과가 가장 좋았다. Sour liquid ferments를 제빵제조시 중종 (sponge dough)에 넣었을 때 반죽의 pH를 4.64까지 낮추어 주어 발효력을 증가시켰고, total titratable acidity (TTA)도 보통 식빵의 0.46%보다 높은 0.54%를 나타내었다. 품질평가에 있어서, sour dough liquid를 이용한 식빵의 경우가 보통식빵보다 비용적(specific volume)이 좋았다. 맛과 외형 특히 관능적인 품질(향취)이 증가되었다. 이는 유산균이 생성한 초산, 젖산 및 아미노산 등이 영향을 미치기 때문으로 보인다. 제품을 25℃에서 6일동안 저장하면서 노화도를 측정한 결과 보통식빵 보다 노화지연에 훨씬 효과적으로 나타났고 Sour liquid ferments는 유산균 등이 생성한 유기산들이 곰팡이의 생육을 억제하여 제품의 저장기간이 연장되었다. The effects of sour liquid ferments with lactic acid bacteria on the baking properties and qualities of White Pan Bread were studied. The mixed culture of Lactobacillus brevis and Lactobacillus plantarum had higher acid equivalents and lower pH-values than single or mixed culture of other lactic acid bacteria which had been used for traditional sour dough bread. Optimum conditions of the incubation of lactic acid bacteria, which are incubation temperature, time and culture medium compositions for lactic fermentation, were also investigated to find out optimum activity for good bread making. The mixed culture of L. brevis and L. plantarum incubated for 24 hours at 30℃ had the most optimum activity for bread manufacturing process and the qualities of the products. The addition of sour liquid ferments to the sponge dough effected on fermentation activity of the sponge dough to lower the level of pH to 4.64 and to produce more total titratable acidity(TTA) of 0.54%, whereas conventional sponge dough bread had 0.46% of TTA. On comparison with control bread, the bread made with sour liquid ferments was found to have better specific volume, taste, symmetry, especially, organoleptic characteristics due to lactic acid, acetic acid and amino acid produced by lactic acid bacteria. Sour dough bread with liquid ferment was considered to be more effective to the inhibition of staling during storage for 6 days at 25℃ and to have longer shelf-life than control.
Performance Enhancement of TFI-OFDM with Path Selection based Channel Identification
Takeshi Yoshimura,Chang-Jun Ann,Takeshi Kamio,Hisato Fujisaka,Kazuhisa Haeiwa 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7
Recently time-frequency interferometry (TFI)-OFDM has been proposed as a channel identification scheme. TFI-OFDM system can multiplex the same impulse response in twice on the time domain without overlapping to each other. In this case, the required pilot signal is only one. Moreover, by averaging of these impulse responses, the accurated channel impulse responses are obtained. However, if the total channel paths are reduced, the performance might be degraded. This is because the channel identification of TFI-OFDM is operated with averaging the selected spectrum signals from the time windows. For the case with reduced channel paths, the selected time spectrum signals include the noise terms. By applying the FFT operation, these noise terms are spread in the frequency domain. In this case, the channel identification is poorly operated due to the noise. To reduce this problem, in the paper, we propose the channel identification method with path selection for performance enhancement of TFI-OFDM.
( Hea Won Ann ),( Jae Kyung Kim ),( Heun Choi ),( Su Jin Jeong ),( Sun Bean Kim ),( Nam Su Ku ),( Sang Hoon Han,),( Chang Oh Kim ),( Jun Yong Choi ),( Young Goo Song ),( June Myung Kim ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
Objectives: The early-goal directed therapy (EGDT) is the current standard approach in severe sepsis patients, which can ameliorate the survival rate up to 10-15 percent. Stress-induced cardiomyopathy (SCMP) is regarded as an acute reversible cardiomyopathy that mimics acute coronary syndrome and increasingly being observed during intensive care with critical ill patients. However, there is no known whether the development of SCMP can affect the clinical outcome in severe sepsis patients received EGDT. Methods: The retrospective cohort study was performed in single center, Severance Hospital. We enrolled total 144 severe sepsis patients with more than 20-years old who received EGDT and transthoracic echocardiography (TTE) within 3 days after emergency department arrival during from January 2008 to June 2012. The demographic, clinical, and laboratory data including EGDT-associated parameters was retrospectively collected from electric medical record. The primary end-point was the 28-day all-cause mortality. The diagnosis of SCMP was defined through the following modified ‘Proposed Mayo Clinic criteria’: (1) an acute cardiac event typically presenting with chest pain and/or dyspnea; (2) transient systolic dysfunction with marked LV contraction abnormality; (3) new ECG abnormalities or modest elevation in cardiac troponin level. Results: The SCMP was identified in 29 (20.1%) patient. The frequency of male and history of diabetes mellitus in patients with SCMP was higher than those in patients without SCMP (72.4% vs. 50.4%, p=0.038 and 48.3% vs. 25.7%, p=0.024, respectively). The patients with SCMP had the higher levels of delta neutrophil index and APACHE II score (27.8±24.1 vs. 14.7±15.4, p=0.006 and 23.3±6.8 vs. 19.5±7.1, p=0.016, respectively). The 7- and 28-day all-cause mortalities were higher in patients with SCMP compared with patients without SCMP (20.7% vs. 6.2%, p=0.026, 37.9% vs. 18.6%, p=0.044). In the Cox`s regression model, the presence of SCMP was the independent factor associated with 28-day all-cause mortality (OR, 2.7, 95% CI 1.8-8.6, p=0.034). Conclusions: The development of SCMP in spite of successful management by EGDT in severe sepsis patients can increase all-cause mortality.
S-253 : Experience of Elizabethkingia meningoseptica Infection in a Tertiary Hospital
( Sun Young Ann ),( Sung Hyeok Ryou ),( Seong Jun Park ),( Hyun Don Joo ),( Chang Hyun Park ),( Ji Sung Choi ),( Doh Hyung Kim ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
Background: There have been limited number of reports documenting patients with Elizabethkingia meningoseptica (Chryseobacterium meningoseptica) infection in Korea. The knowledge of clinical and antimicrobial characteristics are still scarce. Methods: Medical records of patients with Elizabethkingia meningoseptica infection in a tertiary university hospital from March 1, 2006 to February 28, 2013 were retrospectively reviewed. The clinical features and antimicrobial susceptibilities of these patients were analyzed. Results: A total of 30 patients were identified to have Elizabethkingia meningoseptica infection. Median age of patients was 68.5 years, and male gender was more frequent (17, 56.7%). The most common site of isolation was sputum (23, 76.7%) and pneumonia was the most common type of infection (21, 70%). Prolonged hospitalization ≥ 28 days (26, 86.7%), prior intensive care unit stay (23, 76.7%), mechanical ventilation (23, 76.7%), and tracheostomy state (18, 60%) were most commonly observed in these patients. In-vitro antimicrobial susceptibility tests showed minocycline (27, 90%) and fluoroquinolones including levofloxacin (20, 66.7%) and ciprofloxacin (18, 60%) were most frequently susceptible. However, appropriate antibiotics based on culture reports were only provided for 8 (26.7%) patients and the overall mortality presumptive on Elizabethkingia infection was 20% (6/30). Conclusion: Elizabethkingia meningoseptica was most frequently isolated from respiratory specimen and usually manifested as respiratory infection. Minocycline and fluoroquinolones were most susceptible antibiotics in in-vitro tests. Although the mortality of infection by this organism was quite high, the rate of appropriate antibiotic use based on susceptibility test results was notably low.