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Removing Lipemia in Serum/Plasma Samples: A Multicenter Study
María-José Castro-Castro,Beatriz Candás-Estébanez,Margarita Esteban-Salán,Pilar Calmarza,Teresa Arrobas-Velilla,Carlos Romero-Román,Miguel Pocoví-Mieras,José-Ángel Aguilar-Doreste,Sociedad Española de 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.6
Background: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Methods: Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods—LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). Results: When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
THE EVOLUTION OF ASTRONOMICAL OBSERVATORY DESIGN
Miguel Ángel Castro Tirado,Alberto J. Castro-Tirado 한국천문학회 2019 Journal of The Korean Astronomical Society Vol.52 No.4
This work addresses the development of the astronomical observatory all through history, from an architectural point of view, as a building in relation to the observing instruments and their functioning as a heterogeneous work center. We focused on 32 observatories (in the period 1259–2007) and carefully analyzed the architectures. Considering the impact of the construction itself or its facilities on the results of the research (thermal or structural stability, poor weather protection, turbulence, etc.), there is little attention paid to theories or studies of the architectural or construction aspects of the observatories. Therefore, this work aims to present a theoretical-critical contribution that, at least, invites the reflection of those involved in the development of astronomical observatories in the future.
Gustavo Batista de Castro Souza,Flávio Henrique Teles Vieira,Cláudio Ribeiro Lima,Getúlio Antero de Deus Júnior,Marcelo Stehling de Castro,Sérgio Granato de Araujo,Thiago Lara Vasques 한국전자통신연구원 2016 ETRI Journal Vol.38 No.1
Smart metering systems have become widespread around the world. RF mesh communication systems have contributed to the creation of smarter and more reliable power systems. This paper presents an algorithm for positioning GPRS concentrators to attain delay constraints for a ZigBee-based mesh network. The proposed algorithm determines the number and placement of concentrators using integer linear programming and a queueing model for the given mesh network. The solutions given by the proposed algorithm are validated by verifying the communication network performance through simulations.
Castro, Ruth M.,Moreira, Lisela,Rojas, Maria R.,Gilbertson, Robert L.,Hernandez, Eduardo,Mora, Floribeth,Ramirez, Pilar The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.3
Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ~1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ~580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.
SOME ASPECTS OF ZARISKI TOPOLOGY FOR MULTIPLICATION MODULES AND THEIR ATTACHED FRAMES AND QUANTALES
Castro, Jaime,Rios, Jose,Tapia, Gustavo Korean Mathematical Society 2019 대한수학회지 Vol.56 No.5
For a multiplication R-module M we consider the Zariski topology in the set Spec (M) of prime submodules of M. We investigate the relationship between the algebraic properties of the submodules of M and the topological properties of some subspaces of Spec (M). We also consider some topological aspects of certain frames. We prove that if R is a commutative ring and M is a multiplication R-module, then the lattice Semp (M/N) of semiprime submodules of M/N is a spatial frame for every submodule N of M. When M is a quasi projective module, we obtain that the interval ${\uparrow}(N)^{Semp}(M)=\{P{\in}Semp(M){\mid}N{\subseteq}P\}$ and the lattice Semp (M/N) are isomorphic as frames. Finally, we obtain results about quantales and the classical Krull dimension of M.
Castro-Perez, B.I.,Garzon-Proano, J.S.,Lopez-Soto, M.A.,Barreras, A.,Gonzalez, V.M.,Plascencia, Alejandro,Estrada-Angulo, A.,Davila-Ramos, H.,Rios-Rincon, F.G.,Zinn, R.A. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.8
Four male lambs (Katahdin; average live weight $25.9{\pm}2.9$ kg) with "T" type cannulas in the rumen and proximal duodenum were used in a $4{\times}4$ Latin square experiment to evaluate the influence of supplemental dry distillers grain with solubles (DDGS) levels (0, 10, 20 and 30%, dry matter basis) in substitution for dry-rolled (DR) corn on characteristics of digestive function and digestible energy (DE) of diet. Treatments did not influence ruminal pH. Substitution of DR corn with DDGS increased ruminal neutral detergent fiber (NDF) digestion (quadratic effect, p<0.01), but decreased ruminal organic matter (OM) digestion (linear effect, p<0.01). Replacing corn with DDGS increased (linear, $p{\leq}0.02$) duodenal flow of lipids, NDF and feed N. But there were no treatment effects on flow to the small intestine of microbial nitrogen (MN) or microbial N efficiency. The estimated UIP value of DDGS was 44%. Postruminal digestion of OM, starch, lipids and nitrogen (N) were not affected by treatments. Total tract digestion of N increased (linear, p = 0.04) as the DDGS level increased, but DDGS substitution tended to decrease total tract digestion of OM (p = 0.06) and digestion of gross energy (p = 0.08). However, it did not affect the dietary digestible energy (DE, MJ/kg), reflecting the greater gross energy content of DDGS versus DR corn in the replacements. The comparative DE value of DDGS may be considered similar to the DE value of the DR corn it replaced up to 30% in the finishing diets fed to lambs.
Lipoinjection with Adipose Stem Cells for Nasal Modeling: Rhino Cell, a Highly Versatile Alternative
Castro-Govea Yanko,Garcia Garza Jorge Alberto,Vazquez Lara Sergio Eduardo,Gonzalez Cantu Cynthia Minerva,Chacon Moreno Hernan Jesus,Cervantes Kardash Victor Hugo 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.4
It is undeniable that a significant number of patients who want to improve their facial appearance is increasingly interested in nonsurgical procedures. Without a doubt, the use of autologous fat could not be left out as a magnificent alternative for nasal modeling simply because of four influential factors: ease of collection, compatibility, the temporality of the results, and safety. This work describes an innovative alternative technique for nasal modeling using micrografts enriched with adipose-derived mesenchymal stem cells (ASCs). With this technique, fat was collected and divided into two samples, nanofat and microfat. Nanofat was used to isolate the ASCs; microfat was enriched with ASCs and used for nasal modeling. Lipoinjection was performed in a supraperiosteal plane on the nasal dorsum. Through a retrolabial access, the nasal tip and base of the columella were lipoinjected. We consider that nonsurgical nasal modeling using micrografts enriched with ASCs can be an attractive and innovative alternative. This technique will never be a substitute for surgical rhinoplasty. It can be performed in a minor procedure area with rapid recovery and return to the patient’s daily activities the next day. If necessary, the procedure can be repeated.
First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon
Castro, Marcela dos Santos,David, Maridelzira Betania Moraes,Goncalves, Evonnildo Costa,Siqueira, Andrei Santos,Virgulino, Rodrigo Rodrigues,Aguiar, Delia Cristina Figueira The Korean Society of Veterinary Science 2022 JOURNAL OF VETERINARY SCIENCE Vol.23 No.1
Background: Canine herpesvirus type 1 (CaHV-1) infects dogs and is associated with neonatal deaths and reproductive, ocular, neurological, and respiratory problems. In Brazil, reports of CaHV-1 have been restricted to the southeast and south regions, particularly in municipalities in the state of Rio Grande do Sul. Objectives: To assess the presence and variability of CaHV-1 in canine populations in the state of Pará, North Brazil. Methods: Biological samples from 159 dogs from 4 municipalities in the State of Pará were evaluated using polymerase chain reaction and phylogenetic analyses, with the target being the viral enzyme, thymidine kinase. Results: CaHV-1 was detected in 13 dogs (8.2%), with 2 animals being from the municipality of Santa Bárbara do Pará, 8 from Algodoal Island, 2 from Salinópolis, and one from Capanema. The study sequences revealed 100% identity among themselves and 64% to 100% identity with the other nucleotide sequences from Australia, Brazil, United Kingdom, and United States, including 100% identity with the 2002 isolate from Australia. The 1996 isolate from France was grouped in a branch that was different from the sequence of this study. Conclusions: This study presents the first molecular detection of CaHV-1 in dogs from the Amazon region in northern Brazil. The nucleotide identity between the strains and cytosine insertion in the sequences isolated in this study suggests at least 2 strains of CaHV-1 circulating in Brazil (Pará and BTU-1).
Cell-Assisted Lipotransfer for the Treatment of Parry-Romberg Syndrome
Castro-Govea, Yanko,De La Garza-Pineda, Oscar,Lara-Arias, Jorge,Chacon-Martinez, Hernan,Mecott-Rivera, Gabriel,Salazar-Lozano, Abel,Valdes-Flores, Everardo Korean Society of Plastic and Reconstructive Surge 2012 Archives of Plastic Surgery Vol.39 No.6
Progressive facial hemiatrophy, also known as Parry-Romberg syndrome, is a progressive and self-limited deformation of the subcutaneous tissue volume on one side of the face that creates craniofacial asymmetry. We present the case of a patient with a five-year history of progressive right facial hemiatrophy, who underwent facial volumetric restoration using cell-assisted lipotransfer (CAL), which consists of an autologous fat graft enriched with adipose-derived stem cells (ASCs) extracted from the same patient. ASCs have the capacity to differentiate into adipocytes. They also promote angiogenesis, release angiogenic growth factors, and some can survive as stem cells. The use of autologous fat as a filler in soft tissue atrophy has been satisfactory in patients with mild and moderate Parry-Romberg syndrome. Currently, CAL has showed promising results in the long term by decreasing the rate of fat reabsorption. The permanence and stability of the graft in all the injected areas has showed that autologous fat grafts enriched with stem cells could be a promising technique for the correction of defects caused by this syndrome.