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Flood Disaster Risk Simulation in Urban Areas in Chaclacayo district, Peru
Castillo Osorio, Ever Enrique,Yoo, Hwan Hee 대한공간정보학회 2018 대한공간정보학회지 Vol.26 No.2
Many urban areas are vulnerable to hazards produced by natural disasters specially floods that are currently increasing. For this reason, prevention efforts must focus on reducing disaster risks when they happen. These actions are applied to land areas; therefore the geographic data are valuable to improve them, because they give the spatial location of the elements in the terrain. Likewise, the hydrological simulation models provide a perspective of the possible vulnerable areas impacted by floods. In order to achieve the improvement of flood disaster risk prevention activities, we propose a methodology for the hydrological simulation of flood scenarios using HecRAS tool. In this methodology we define the geometrical conditions of the cartographic data, use Gumbel`s distribution method for the processing of hydrological data, and develop the hydrological simulation process applying geomorphological and fluid mechanics parameters. The results of the hydrological simulation and the cartographic data are stored in a flexible geodatabase designed by us, using standards and protocols based on open source, which allows their collection, processing and dissemination. We apply the proposed methodology to a case study and develop the hydrological modeling for three simulation scenarios, to calculate the flooding urban areas in Chaclacayo district located in Peru.
Castillo, Rochelle,Sheth, Khushboo,Babigian, Alan,Scola, Christopher Korean Society of Plastic and Reconstructive Surge 2018 Archives of Plastic Surgery Vol.45 No.5
While the success or failure of carpal tunnel release ultimately depends on the interplay of a wide array of factors, a broad understanding of the normal anatomy of the carpal tunnel accompanied by awareness of the possible variations of the individual structures that make up its contents is crucial to optimizing surgical outcomes. While anatomic variants such as extension of the flexor digitorum muscle bellies have been described as a cause of primary carpal tunnel syndrome (CTS), there have been no reports depicting its association with recurrent CTS following initially successful carpal tunnel release, a finding with potentially significant prognostic implications that can aid in operative planning. In such cases where muscle extension is identified preoperatively, careful debulking of the muscle belly may be beneficial in improving long-term surgical outcomes.
Characterization and functional analysis of two PKR genes in fugu (<i>Takifugu rubripes</i>)
del Castillo, Carmelo S.,Hikima, Jun-ichi,Ohtani, Maki,Jung, Tae-Sung,Aoki, Takashi Elsevier 2012 FISH AND SHELLFISH IMMUNOLOGY Vol.32 No.1
<P><B>Abstract</B></P><P>PKR (protein kinase R) is a serine–threonine kinase that inhibits protein synthesis by the phosphorylation of the eukaryotic translation initiation factor 2-alpha (eIF2α), and activates NFκB by inducing NFκB-inducing kinase and IκB (inhibitor of NFκB) kinase. This can lead to antiviral and anti-proliferative effects. In this study, the complete sequence and organization of two fugu PKR genes (fPKRs) were determined by <I>in silico</I> analysis and conventional PCR. The full-length <I>fPKR1</I> and <I>fPKR2</I> genes were 3832 bp and 4325 bp, which encoded 523 and 492 amino acids, respectively. Both encoded two dsRNA binding domains and a Serine/Threonine protein kinase domain, and showed very high similarity to green spotted puffer PKRs. Gene expression of the two fPKRs was measured by quantitative real-time PCR on tissue samples from healthy fish and peripheral blood leukocytes stimulated with polyinosinic:polycytidylic acid (PolyI:C) or lipopolysaccharides (LPS). The fPKRs were highly expressed in the skin and fPKR2 was significantly induced in PBLs by PolyI:C but not by LPS. The fPKRs inhibited translation of a luciferase reporter gene in a dose-dependent manner and induced transcriptional activity of a mammalian NFκB luciferase reporter. These results demonstrate that two PKRs in a single species can both be independently, but not equally, functional and support the hypothesis that fish PKRs have roles in the innate immune response similar to those of mammalian PKRs.</P> <P><B>Highlights</B></P><P>► The complete sequence and structure of two PKR genes in fugu were determined. ► The fugu PKRs were highly expressed in the skin of healthy fish. ► The fugu PKRs were demonstrated to inhibit luciferase activity and induce NFκB.</P>
Angel Castillo-Corujo,Yuko Uchida,Mirva J. Saaranen,Lloyd W. Ruddock The Korean Society for Microbiology and Biotechnol 2024 Journal of microbiology and biotechnology Vol.34 No.5
The production of disulfide bond-containing recombinant proteins in Escherichia coli has traditionally been done by either refolding from inclusion bodies or by targeting the protein to the periplasm. However, both approaches have limitations. Two broad strategies were developed to allow the production of proteins with disulfide bonds in the cytoplasm of E. coli: i) engineered strains with deletions in the disulfide reduction pathways, e.g. SHuffle, and ii) the co-expression of oxidative folding catalysts, e.g. CyDisCo. However, to our knowledge, the relative effectiveness of these strategies has not been properly evaluated. Here, we systematically compare the purified yields of 14 different proteins of interest (POI) that contain disulfide bonds in their native state when expressed in both systems. We also compared the effects of different background strains, commonly used promoters, and two media types: defined and rich autoinduction. In rich autoinduction media, POI which can be produced in a soluble (non-native) state without a system for disulfide bond formation were produced in higher purified yields from SHuffle, whereas all other proteins were produced in higher purified yields using CyDisCo. In chemically defined media, purified yields were at least 10x higher in all cases using CyDisCo. In addition, the quality of the three POI tested was superior when produced using CyDisCo.