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- 검색키워드
- 저자 : Boopathi, Thangavelu (검색결과 27 건)
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Boopathi, T.,Faria, D. G.,Lee, M. D.,Lee, J.,Chang, M.,Ki, J. S. Springer Science + Business Media 2015 Polar biology Vol.38 No.2
<P>The molecular diversity of freshwater microeukaryotes, particularly phytoplankton, in the Arctic Svalbard, has been relatively unexplored. Freshwater algae are considered biological indicators of environmental change and can be useful in assessing the impact of global climate change and increased environmental pollution. In this study, freshwater microeukaryotes in an Arctic reservoir at Ny-C(0)lesund (Svalbard, Norway) were studied using the hypervariable V1-V3 small subunit rRNA and 454 pyrosequencing. On the basis of 8,956 reads, we revealed high genetic diversity in eukaryotes, representing all known eukaryotic supergroups, except Excavata. 'Chromalveolata' (previously supergroup Chromalveolata) and Archaeplastida were the most and least abundant supergroups, respectively. After data mining, 57 phylotypes were detected from 7,398 pyrosequences. They were dominated by stramenopiles (84 %) and Dinoflagellata (13 %), with minor contributions from Cryptophyta, Chlorophyta, and Telonemida. The detection of algae belonging to the orders Mamiellales and Monomastigales provides a window into a fraction of the 'rare biosphere' that had previously been undetected in such environments. Interestingly, no haptophytes were recorded. Stramenopiles and Dinoflagellata mainly comprised taxa belonging to the families Chrysophyceae, Synurophyceae, and Dinophyceae. On the basis of the proportion of operational taxonomic units, the dominant phylotypes were found to include Ochromonas spp., Mallomonas spp., and Uroglena americana. These results demonstrate the significance of a chrysophyte-dominated microeukaryotic community, which is of great potential for future studies in terms of reconstruction of past climate trends and monitoring of environmental change in the Arctic.</P>
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Boopathi, T.,Lee, J.B.,Youn, S.H.,Ki, J.S. Pergamon Press ; Elsevier Science Ltd 2015 Biochemical systematics and ecology Vol.63 No.-
The East China Sea (ECS) has long been considered an important monitoring site for oceanic ecosystem changes because many water currents and river discharges constantly influence this area. In this study, the community structure and diversity of phytoplankton in the northern part of the ECS adjacent to Jeju Island were explored using small subunit ribosomal RNA (SSU) pyrosequencing. We analysed samples collected from four stations from the surface and at 30-m and 50-m depths during April and September 2011. We observed spatial and temporal variations in the phytoplankton community. Among phytoplankton, diatoms and dinoflagellates constituted a major portion at all stations (60-90%). However, comparison of the April and September samples showed seasonal variation and shifts in the dispersion of diatom and dinoflagellates among stations. Among stations, diatoms dominated St. 1 and others were dominated by dinoflagellates. Furthermore, phylotypes of potentially toxin-producing genera such as Karlodinium, Heterocapsa, Gymnodinium, Gyrodinium, and Pseudo-nitzschia were dominant in this area.
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Till 2018: a survey of biomolecular sequences in genus Panax
Boopathi, Vinothini,Subramaniyam, Sathiyamoorthy,Mathiyalagan, Ramya,Yang, Deok-Chun The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Ginseng is popularly known to be the king of ancient medicines and is used widely in most of the traditional medicinal compositions due to its various pharmaceutical properties. Numerous studies are being focused on this plant's curative effects to discover their potential health benefits in most human diseases, including cancer- the most life-threatening disease worldwide. Modern pharmacological research has focused mainly on ginsenosides, the major bioactive compounds of ginseng, because of their multiple therapeutic applications. Various issues on ginseng plant development, physiological processes, and agricultural issues have also been studied widely through state-of-the-art, high-throughput sequencing technologies. Since the beginning of the 21st century, the number of publications on ginseng has rapidly increased, with a recent count of more than 6,000 articles and reviews focusing notably on ginseng. Owing to the implementation of various technologies and continuous efforts, the ginseng plant genomes have been decoded effectively in recent years. Therefore, this review focuses mainly on the cellular biomolecular sequences in ginseng plants from the perspective of the central molecular dogma, with an emphasis on genomes, transcriptomes, and proteomes, together with a few other related studies.
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Boopathi, Thangavelu,Wang, Hui,Lee, Man-Duck,Ki, Jang-Seu Korean Society of Environmental Biology 2018 환경생물 : 환경생물학회지 Vol.36 No.3
The incidence of freshwater algal bloom has been increasing globally in recent years and poses a major threat to environmental health. Cyanobacteria are the major component of the bloom forming community that must be monitored frequently. Their morphological identities, however, have remained elusive, due to their small size in cells and morphological resemblances among species. We have analyzed molecular diversity and seasonal changes of cyanobacteria in Paldang Reservoir, Korea, using morphological and 16S rRNA pyrosequencing methods. Samples were collected at monthly intervals from the reservoir March-December 2012. In total, 40 phylotypes of cyanobacteria were identified after comparing 49,131 pyrosequence reads. Cyanobacterial genera such as Anabaena, Aphanizomenon, Microcystis and Synechocystis were predominantly present in samples. However, the majority of cyanobacterial sequences (65.9%) identified in this study were of uncultured origins, not detected morphologically. Relative abundance of cyanobacterial sequences was observed as high in August, with no occurrence in March and December. These results suggested that pyrosequencing approach may reveal cyanobacterial diversity undetected morphologically, and may be used as reference for studying and monitoring cyanobacterial communities in aquatic environments.
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Boopathi, Thangavelu,Ki, Jang-Seu CSIRO Publishing 2016 Marine & freshwater research Vol.67 No.11
<P>Environmental monitoring of the succession of phytoplankton communities in freshwater ecosystems is critical in efficient water quality management. In the present study we analysed the monthly dynamics of eukaryotic phytoplankton diversity and community structure in a freshwater reservoir (Paldang Reservoir, South Korea) using small subunit (SSU) rRNA pyrosequencing. We analysed physicochemical and biological parameters of water samples collected at monthly intervals from March 2012 to February 2013. The occurrence of phytoplankton exhibited a monthly variation: low in September (13.2%) and February (9.7%) and high in May (75.5%), July (76.5%), August (86.3%) and November (70.6%). We observed greater phytoplankton diversity predominantly represented by diatoms (37.4%), dinoflagellates (29%), cryptophytes (16%) and chlorophytes (10.4%). In early and mid-spring, diatoms, particularly Stephanodiscus spp., were dominant; however, in late spring and early summer the cryptophyte Cryptomonas spp. was dominant; the dinoflagellate Peridionopsis sp. was dominant in late summer and late autumn. Overall, the molecular results of the present study represent a typical pattern of seasonal phytoplankton succession in temperate regions. Pyrosequencing detected more phytoplankton taxa that were unresolved under microscopy, suggesting the usefulness of this method in continuous monitoring of phytoplankton communities in freshwater ecosystems.</P>
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Thangavelu Boopathi,Hui Wang,Man-Duck Lee,Jang-Seu Ki 한국환경생물학회 2018 환경생물 : 환경생물학회지 Vol.36 No.3
The incidence of freshwater algal bloom has been increasing globally in recent years and poses a major threat to environmental health. Cyanobacteria are the major component of the bloom forming community that must be monitored frequently. Their morphological identities, however, have remained elusive, due to their small size in cells and morphological resemblances among species. We have analyzed molecular diversity and seasonal changes of cyanobacteria in Paldang Reservoir, Korea, using morphological and 16S rRNA pyrosequencing methods. Samples were collected at monthly intervals from the reservoir March-December 2012. In total, 40 phylotypes of cyanobacteria were identified after comparing 49,131 pyrosequence reads. Cyanobacterial genera such as Anabaena, Aphanizomenon, Microcystis and Synechocystis were predominantly present in samples. However, the majority of cyanobacterial sequences (65.9%) identified in this study were of uncultured origins, not detected morphologically. Relative abundance of cyanobacterial sequences was observed as high in August, with no occurrence in March and December. These results suggested that pyrosequencing approach may reveal cyanobacterial diversity undetected morphologically, and may be used as reference for studying and monitoring cyanobacterial communities in aquatic environments.
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Till 2018: a survey of biomolecular sequences in genus Panax
Vinothini Boopathi,Sathiyamoorthy Subramaniyam,MATHIYALAGANRAMYA,Deok-Chun Yang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1
Ginseng is popularly known to be the king of ancient medicines and is used widely in most of thetraditional medicinal compositions due to its various pharmaceutical properties. Numerous studies arebeing focused on this plant’s curative effects to discover their potential health benefits in most humandiseases, including cancerd the most life-threatening disease worldwide. Modern pharmacologicalresearch has focused mainly on ginsenosides, the major bioactive compounds of ginseng, because of theirmultiple therapeutic applications. Various issues on ginseng plant development, physiological processes,and agricultural issues have also been studied widely through state-of-the-art, high-throughputsequencing technologies. Since the beginning of the 21st century, the number of publications on ginsenghas rapidly increased, with a recent count of more than 6,000 articles and reviews focusing notably onginseng. Owing to the implementation of various technologies and continuous efforts, the ginseng plantgenomes have been decoded effectively in recent years. Therefore, this review focuses mainly on thecellular biomolecular sequences in ginseng plants from the perspective of the central molecular dogma,with an emphasis on genomes, transcriptomes, and proteomes, together with a few other related studies.
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Sinduja Boopathy,Merline Sheela Appavoo,Ilamathi Radhakrishnan 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5
To remediate crude oil-contaminated soil, poultry droppings and sunflower seed husk were incorporated at various concentrations. Initially, the pH, moisture content, total organic carbon (TOC), total nitrogen content, and bacterial population of soil, poultry droppings, and sunflower seed husk were determined. The initial bacterial population was 35 × 108 colony forming units/g soil (dry weight basis). The total petroleum hydrocarbon (TPH) content in crude oil-contaminated soil was 7,048.4 ㎎/㎏, in which 857.9 ㎎/㎏ accounted for eicosane. Naphthalene and acenaphthalene were the two polyhydroxy aromatic hydrocarbons present in soil at low concentrations. After the amendment, the pH, moisture content, microbial population, and TPH content of soil were determined on days 20, 40, and 60. The TPH concentration was considerably decreased in the treatment T4 wherein 250 g of soil was mixed with 125 g of poultry droppings and 125 g of sunflower seed husk. The indigenous bacterial population was also increased tremendously. The dehydrogenase enzyme activity was increased in the amended soil (T4: 0.74 ± 0.06 ㎍ TPF/g/h). After the treatment of soil, the germination percentage and vigour index of maize, lady’s finger and tomato seeds were enhanced.
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Ocular promoting activity of grape polyphenols—A review
Natarajan, Sithranga Boopathy,Hwang, Jin-Woo,Kim, Yon-Suk,Kim, Eun-Kyung,Park, Pyo-Jam Elsevier 2017 Environmental toxicology and pharmacology Vol.50 No.-
<P><B>Abstract</B></P> <P>The eye is a sensitive organ with complex optical system involves in the perception of light. Although it has several protective mechanisms by itself, various physiological and metabolic disorders are detrimental to the proper functioning of the visual system. Grape juice has long been used worldwide for its potent medicinal values including ocular promotion. Bioactivities of grape products are highly attributed to the presence of health promoting phytochemicals in them. Some phytochemicals present in the grape juice have been involved in the maintenance of intra-ocular pressure, regulation of glucose metabolisms and suppression of pro-inflammatory cytokines in the system. Particularly, the grape derived phytochemicals involve in minimizing various eye defects such as macular degradation, uvea, cataract formation, red eye, diabetic retinopathy and so on. However, only limited number of studies has been conducted so far focusing the ocular promoting activity of grape polyphenols. In this review, we discuss the role of grape polyphenols in ocular promotion relating their anti-oxidant, anti-microbial, anti-aging, anti-hypertensive and anti-inflammatory properties.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Design of Grey-Verhulst Sliding Mode Controller for Antilock Braking System
A. Manivanna Boopathi,A. Abudhahir 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.3
In this paper, an adaptive controller called Grey-Verhulst Sliding Mode Controller (GVSMC) is proposedfor the laboratory Antilock Braking System (ABS). The developed Grey-Verhulst Model (GVM) does a betterprediction of wheel slip than a simple Grey Model. The first order Sliding Mode Controller (SMC) maintains thewheel slip at the desired value. By combining the GVM and SMC, the resulting GVSMC controls the wheel slipat the desired optimum value at which the vehicle control, non-skidding and steerability are ensured during suddenbraking. The proposed controller also reduces the stopping distance considerably. Simulation results show that theperformance of the proposed GVSMC is better than the simple SMC and Grey SMC reported in literature earlier. Change in road conditions has also been considered.
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