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Boopathi, Thangavelu,Ki, Jang-Seu CSIRO Publishing 2016 Marine & freshwater research Vol.67 No.11
<P>Environmental monitoring of the succession of phytoplankton communities in freshwater ecosystems is critical in efficient water quality management. In the present study we analysed the monthly dynamics of eukaryotic phytoplankton diversity and community structure in a freshwater reservoir (Paldang Reservoir, South Korea) using small subunit (SSU) rRNA pyrosequencing. We analysed physicochemical and biological parameters of water samples collected at monthly intervals from March 2012 to February 2013. The occurrence of phytoplankton exhibited a monthly variation: low in September (13.2%) and February (9.7%) and high in May (75.5%), July (76.5%), August (86.3%) and November (70.6%). We observed greater phytoplankton diversity predominantly represented by diatoms (37.4%), dinoflagellates (29%), cryptophytes (16%) and chlorophytes (10.4%). In early and mid-spring, diatoms, particularly Stephanodiscus spp., were dominant; however, in late spring and early summer the cryptophyte Cryptomonas spp. was dominant; the dinoflagellate Peridionopsis sp. was dominant in late summer and late autumn. Overall, the molecular results of the present study represent a typical pattern of seasonal phytoplankton succession in temperate regions. Pyrosequencing detected more phytoplankton taxa that were unresolved under microscopy, suggesting the usefulness of this method in continuous monitoring of phytoplankton communities in freshwater ecosystems.</P>
Thangavelu Boopathi,Hui Wang,Man-Duck Lee,Jang-Seu Ki 한국환경생물학회 2018 환경생물 : 환경생물학회지 Vol.36 No.3
The incidence of freshwater algal bloom has been increasing globally in recent years and poses a major threat to environmental health. Cyanobacteria are the major component of the bloom forming community that must be monitored frequently. Their morphological identities, however, have remained elusive, due to their small size in cells and morphological resemblances among species. We have analyzed molecular diversity and seasonal changes of cyanobacteria in Paldang Reservoir, Korea, using morphological and 16S rRNA pyrosequencing methods. Samples were collected at monthly intervals from the reservoir March-December 2012. In total, 40 phylotypes of cyanobacteria were identified after comparing 49,131 pyrosequence reads. Cyanobacterial genera such as Anabaena, Aphanizomenon, Microcystis and Synechocystis were predominantly present in samples. However, the majority of cyanobacterial sequences (65.9%) identified in this study were of uncultured origins, not detected morphologically. Relative abundance of cyanobacterial sequences was observed as high in August, with no occurrence in March and December. These results suggested that pyrosequencing approach may reveal cyanobacterial diversity undetected morphologically, and may be used as reference for studying and monitoring cyanobacterial communities in aquatic environments.
Boopathi, Thangavelu,Wang, Hui,Lee, Man-Duck,Ki, Jang-Seu Korean Society of Environmental Biology 2018 환경생물 : 환경생물학회지 Vol.36 No.3
The incidence of freshwater algal bloom has been increasing globally in recent years and poses a major threat to environmental health. Cyanobacteria are the major component of the bloom forming community that must be monitored frequently. Their morphological identities, however, have remained elusive, due to their small size in cells and morphological resemblances among species. We have analyzed molecular diversity and seasonal changes of cyanobacteria in Paldang Reservoir, Korea, using morphological and 16S rRNA pyrosequencing methods. Samples were collected at monthly intervals from the reservoir March-December 2012. In total, 40 phylotypes of cyanobacteria were identified after comparing 49,131 pyrosequence reads. Cyanobacterial genera such as Anabaena, Aphanizomenon, Microcystis and Synechocystis were predominantly present in samples. However, the majority of cyanobacterial sequences (65.9%) identified in this study were of uncultured origins, not detected morphologically. Relative abundance of cyanobacterial sequences was observed as high in August, with no occurrence in March and December. These results suggested that pyrosequencing approach may reveal cyanobacterial diversity undetected morphologically, and may be used as reference for studying and monitoring cyanobacterial communities in aquatic environments.